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The map label for this gene is nrfC [H]

Identifier: 221636035

GI number: 221636035

Start: 621256

End: 622788

Strand: Reverse

Name: nrfC [H]

Synonym: trd_A0629

Alternate gene names: 221636035

Gene position: 622788-621256 (Counterclockwise)

Preceding gene: 221636036

Following gene: 221636034

Centisome position: 67.86

GC content: 63.99

Gene sequence:

>1533_bases
ATGGCGCGCTATGGGTTCGTCATCGACCAGCGCAAATGCATCGGCTGTCATGCCTGCACGGTCGCTTGTAAATCGGAGAA
CCTCGTTCCGCTCGGGGTCTACCGGACATGGGTGAAGTACGTCGAAAAGGGGACGTTCCCACATACCAGGCGATCGTTCA
CGGTACTGCGCTGCAATCACTGCGACGATGCGCCCTGCGTGACGATTTGCCCAACCAAGGCGCTCTTCCGTCGACCGGAC
GGCATCGTCGACTTCGATGCCGACCGGTGCATCGGCTGCAAGTCGTGTATGCAAGCGTGCCCCTATGATGCGCTCTACAT
CGATCCGATCACCCGGACAGCAGCCAAATGCAATTACTGCAGTCACCGGGTCGATCAAGGCCTGCTTCCAGCCTGTGTCG
TCGTCTGTCCCGAGAAAGCGATCATCGCGGGAGACCTCGACGACCCGACGAGCGAGATCCATCAGCTCGTTGCCCGCGAA
CCGGTGATGGTGCGCAAGCCGGAGCAGGGGACGCGCCCCAAGTTGTTCTACCTCGGGGCGGACGAAGCAAGTCTCACACC
CGAGGTGCAGACGCGGACGAACGGGTACCTGTGGGCTGAGGTGCGGCCGGACGATCGCCCGGCCGAGGCAGTGCGTGGCA
CCGAGTATCTCCCGGGGCCAGCCGATGCGCGGGTAGCCTATGATGTCTGGCATCCCAAACCGTGGGGATGGAAGGTCGCT
AGTTATCTCTGGACGAAGTCGATCGGAAGCGGTGCGCTGGCGTTGGGTGGGCCGTTGCTCGCCAGTGGCGTGATGAGCGA
TCGGCTGCTCGGGATCGGAGCAGCGGCGATCGGGATGGTGTTCCTGGCCATCACCGCCGTGCTTCTGATCTGGGACCTCA
AGCGGCCGGAGCGGTTCTGGTATCTATTGGTCAAACCGAATCCACGGAGTTGGCTCGTCTGGGGCGGCTACATCCTCCTG
GTGGTGGGAGGTCTCGCGTTCTTCTGGGCTGTTGCGTCGCTCGCCGGCTGGCAGGGCATCGAGCGCCTGCTCGCCTGGCT
CGCGCTGCCGGGCGGGATCGCGGCGGCGGGTTACACGGCTTTTCTCTTCGGTCAGGCGGAGGGGCGCGACTTCTGGCAGA
GCCCGCTCTTACTTCCCATCCTGCTCGTGCAAGCGTTGGTGGCCGGTACGGCTGCTCTCCTGTTGCTGTTGGTCGCGGGC
GGAGCACTCGAGGGGCGAGCGTGGCTCGGCCTGGTGTTGGGTGGTGGGTTGATCGGGCTGCTCGTGCTGGTAGCCAGTGA
ACTCTTGGTTCCGCACGCCAACCTGCATGTCGCCAAGGCTGCTCGCCTGCTGACGCATGGCCCCTATCGTGGAGAGCTGC
TCGTGGTCGGCCTTGGTGTCGGTGTGCTGGTACCCCTGATCGCGCTGGCTCTCGGATGGGCGAGTGGGAATCTCGCGCCC
TGGAGCGTTGTCGCGGCGGTCGCAGCCCTGATCGGGCTTTGGAGTTACGAGCGACTCTGGGTCGAGGCCGGGCAAGATAT
CCCGTTGAGTTGA

Upstream 100 bases:

>100_bases
TGTTGGAACGCTGCTTTCTCGACGGAACACCACCTGCGCCGCAGCAACTGGCGCGCGCTCTCAAAGCGGTGATCCGCGGT
GGACGATGAGGGGGACGCCG

Downstream 100 bases:

>100_bases
GGGGGCGACGATGCAGCGCGAGTCGCGAGCGACGACGATGCAGGCAGAAAGACGTGCTGTCCGGGAACCGTTCGTTCCCG
AGCGGACGAGTGGACTGGCC

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: NA

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MARYGFVIDQRKCIGCHACTVACKSENLVPLGVYRTWVKYVEKGTFPHTRRSFTVLRCNHCDDAPCVTICPTKALFRRPD
GIVDFDADRCIGCKSCMQACPYDALYIDPITRTAAKCNYCSHRVDQGLLPACVVVCPEKAIIAGDLDDPTSEIHQLVARE
PVMVRKPEQGTRPKLFYLGADEASLTPEVQTRTNGYLWAEVRPDDRPAEAVRGTEYLPGPADARVAYDVWHPKPWGWKVA
SYLWTKSIGSGALALGGPLLASGVMSDRLLGIGAAAIGMVFLAITAVLLIWDLKRPERFWYLLVKPNPRSWLVWGGYILL
VVGGLAFFWAVASLAGWQGIERLLAWLALPGGIAAAGYTAFLFGQAEGRDFWQSPLLLPILLVQALVAGTAALLLLLVAG
GALEGRAWLGLVLGGGLIGLLVLVASELLVPHANLHVAKAARLLTHGPYRGELLVVGLGVGVLVPLIALALGWASGNLAP
WSVVAAVAALIGLWSYERLWVEAGQDIPLS

Sequences:

>Translated_510_residues
MARYGFVIDQRKCIGCHACTVACKSENLVPLGVYRTWVKYVEKGTFPHTRRSFTVLRCNHCDDAPCVTICPTKALFRRPD
GIVDFDADRCIGCKSCMQACPYDALYIDPITRTAAKCNYCSHRVDQGLLPACVVVCPEKAIIAGDLDDPTSEIHQLVARE
PVMVRKPEQGTRPKLFYLGADEASLTPEVQTRTNGYLWAEVRPDDRPAEAVRGTEYLPGPADARVAYDVWHPKPWGWKVA
SYLWTKSIGSGALALGGPLLASGVMSDRLLGIGAAAIGMVFLAITAVLLIWDLKRPERFWYLLVKPNPRSWLVWGGYILL
VVGGLAFFWAVASLAGWQGIERLLAWLALPGGIAAAGYTAFLFGQAEGRDFWQSPLLLPILLVQALVAGTAALLLLLVAG
GALEGRAWLGLVLGGGLIGLLVLVASELLVPHANLHVAKAARLLTHGPYRGELLVVGLGVGVLVPLIALALGWASGNLAP
WSVVAAVAALIGLWSYERLWVEAGQDIPLS
>Mature_509_residues
ARYGFVIDQRKCIGCHACTVACKSENLVPLGVYRTWVKYVEKGTFPHTRRSFTVLRCNHCDDAPCVTICPTKALFRRPDG
IVDFDADRCIGCKSCMQACPYDALYIDPITRTAAKCNYCSHRVDQGLLPACVVVCPEKAIIAGDLDDPTSEIHQLVAREP
VMVRKPEQGTRPKLFYLGADEASLTPEVQTRTNGYLWAEVRPDDRPAEAVRGTEYLPGPADARVAYDVWHPKPWGWKVAS
YLWTKSIGSGALALGGPLLASGVMSDRLLGIGAAAIGMVFLAITAVLLIWDLKRPERFWYLLVKPNPRSWLVWGGYILLV
VGGLAFFWAVASLAGWQGIERLLAWLALPGGIAAAGYTAFLFGQAEGRDFWQSPLLLPILLVQALVAGTAALLLLLVAGG
ALEGRAWLGLVLGGGLIGLLVLVASELLVPHANLHVAKAARLLTHGPYRGELLVVGLGVGVLVPLIALALGWASGNLAPW
SVVAAVAALIGLWSYERLWVEAGQDIPLS

Specific function: Probably involved in the transfer of electrons from the quinone pool to the type-c cytochromes [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=177, Percent_Identity=48.5875706214689, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI226510944, Length=149, Percent_Identity=39.5973154362416, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1787122, Length=189, Percent_Identity=33.8624338624339, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI1787872, Length=189, Percent_Identity=33.8624338624339, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI2367245, Length=156, Percent_Identity=34.6153846153846, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1789370, Length=187, Percent_Identity=32.620320855615, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1790326, Length=175, Percent_Identity=34.2857142857143, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI87082180, Length=135, Percent_Identity=37.037037037037, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1787749, Length=175, Percent_Identity=32, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1788811, Length=148, Percent_Identity=33.1081081081081, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI87082179, Length=145, Percent_Identity=34.4827586206897, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789067, Length=185, Percent_Identity=35.1351351351351, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1787740, Length=94, Percent_Identity=34.0425531914894, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1789079, Length=185, Percent_Identity=31.8918918918919, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1787478, Length=100, Percent_Identity=31, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1787963, Length=149, Percent_Identity=25.503355704698, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR017567
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 55089; Mature: 54958

Theoretical pI: Translated: 8.01; Mature: 8.01

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARYGFVIDQRKCIGCHACTVACKSENLVPLGVYRTWVKYVEKGTFPHTRRSFTVLRCNH
CCCCCEEECCCHHCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
CDDAPCVTICPTKALFRRPDGIVDFDADRCIGCKSCMQACPYDALYIDPITRTAAKCNYC
CCCCCEEEECCCHHHHCCCCCCEECCCCCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHH
SHRVDQGLLPACVVVCPEKAIIAGDLDDPTSEIHQLVAREPVMVRKPEQGTRPKLFYLGA
HHHHCCCCCCHHHEECCCCEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEC
DEASLTPEVQTRTNGYLWAEVRPDDRPAEAVRGTEYLPGPADARVAYDVWHPKPWGWKVA
CCCCCCCCCEECCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHH
SYLWTKSIGSGALALGGPLLASGVMSDRLLGIGAAAIGMVFLAITAVLLIWDLKRPERFW
HHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
YLLVKPNPRSWLVWGGYILLVVGGLAFFWAVASLAGWQGIERLLAWLALPGGIAAAGYTA
EEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEE
FLFGQAEGRDFWQSPLLLPILLVQALVAGTAALLLLLVAGGALEGRAWLGLVLGGGLIGL
EEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LVLVASELLVPHANLHVAKAARLLTHGPYRGELLVVGLGVGVLVPLIALALGWASGNLAP
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCH
WSVVAAVAALIGLWSYERLWVEAGQDIPLS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
ARYGFVIDQRKCIGCHACTVACKSENLVPLGVYRTWVKYVEKGTFPHTRRSFTVLRCNH
CCCCEEECCCHHCCCEEEEEEECCCCCEEHHHHHHHHHHHHCCCCCCCCCCEEEEEECC
CDDAPCVTICPTKALFRRPDGIVDFDADRCIGCKSCMQACPYDALYIDPITRTAAKCNYC
CCCCCEEEECCCHHHHCCCCCCEECCCCCCCCHHHHHHHCCCCEEEECCHHHHHHHHHHH
SHRVDQGLLPACVVVCPEKAIIAGDLDDPTSEIHQLVAREPVMVRKPEQGTRPKLFYLGA
HHHHCCCCCCHHHEECCCCEEEECCCCCHHHHHHHHHHCCCCEEECCCCCCCCEEEEEEC
DEASLTPEVQTRTNGYLWAEVRPDDRPAEAVRGTEYLPGPADARVAYDVWHPKPWGWKVA
CCCCCCCCCEECCCCEEEEEECCCCCHHHHHCCCCCCCCCCCCEEEEEECCCCCCCHHHH
SYLWTKSIGSGALALGGPLLASGVMSDRLLGIGAAAIGMVFLAITAVLLIWDLKRPERFW
HHHHHHCCCCCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
YLLVKPNPRSWLVWGGYILLVVGGLAFFWAVASLAGWQGIERLLAWLALPGGIAAAGYTA
EEEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCEE
FLFGQAEGRDFWQSPLLLPILLVQALVAGTAALLLLLVAGGALEGRAWLGLVLGGGLIGL
EEEECCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
LVLVASELLVPHANLHVAKAARLLTHGPYRGELLVVGLGVGVLVPLIALALGWASGNLAP
HHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHHCCCCCCCH
WSVVAAVAALIGLWSYERLWVEAGQDIPLS
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]