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The map label for this gene is yfbT [H]

Identifier: 221636030

GI number: 221636030

Start: 613565

End: 614278

Strand: Reverse

Name: yfbT [H]

Synonym: trd_A0624

Alternate gene names: 221636030

Gene position: 614278-613565 (Counterclockwise)

Preceding gene: 221636031

Following gene: 221636028

Centisome position: 66.93

GC content: 65.41

Gene sequence:

>714_bases
ATGGGTGATGACCGGCGTATGGCCTTCCTGTTCGATCTGGACGGCACACTCGTCGATTCGGTCTACCAGCATGTCGTGGC
CTGGCGGGAAGCGCTGGAGGCGATCGGAATCGAGCTCTCGATCTGGCGGATCCACCGGCGCATCGGGATGAGCGGTGGGC
TGTTTCTCGCTGGTCTCTTGCGCGAGACGGGCCGAGCAGTGACGGCCGAGGAGATCCGGCGCGTGCAGCAGGTTCATGCG
GAGACGTATGGTCGCTTGCTCGGTCAGGTTCGTCCCTTGCCAGGGGCACGGGAACTGCTCGACGAACTGACGCGGCTGGG
AATTCCTTGGGCGATCGCCACGAGTGGCCGGTTGGAGAGTGCGCGCAAGACCGTCGCTGTCTTGGGGTTGGATGCAGACC
AGATCCCGCTCGTGACGCGCGATCATGTGCGGCACGCGAAGCCTGATCCCGATCTCTTCCTCGCGGCAGCCGAGCGTCTC
GGCGTGGAGATCGGGGAGTCGGTGGTGGTTGGGGACAGTGTTTGGGATATGCTGGCCGCCCGCCGCGCACGGGCTTTGAG
CGTTGGCCTGCTTTCGGGCGGCTACGGAGCGGGCGAACTCCTGGAGGCGGGAGCCTACCGGGTGTACGACGATCCGGCCG
ATCTCCTGCGACATCTCGACGAAGTGGGTGTGCGGGCGGCCGATTACGAGTGGACGCTGGCTGAGCCATCCTGA

Upstream 100 bases:

>100_bases
TACAAGGATGTCGAACATGTGGTCGAGGTAATGTGCAATGCCGGTATCGTGGCGCGTGTCGCCCGGTTGCGCCCGATCGT
CTGCCTGAAGGGGTGAGACG

Downstream 100 bases:

>100_bases
GATCCAGAGTGACTGACGGAATGACCCTGTTGCGGTGGTAGAGGAAGGTCGGACAGCCGTCTCGGCTGCGGTGGCATCGT
CAGCTGGTTCGAAGGGATGC

Product: HAD-superfamily hydrolase, subfamily IA, variant 3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MGDDRRMAFLFDLDGTLVDSVYQHVVAWREALEAIGIELSIWRIHRRIGMSGGLFLAGLLRETGRAVTAEEIRRVQQVHA
ETYGRLLGQVRPLPGARELLDELTRLGIPWAIATSGRLESARKTVAVLGLDADQIPLVTRDHVRHAKPDPDLFLAAAERL
GVEIGESVVVGDSVWDMLAARRARALSVGLLSGGYGAGELLEAGAYRVYDDPADLLRHLDEVGVRAADYEWTLAEPS

Sequences:

>Translated_237_residues
MGDDRRMAFLFDLDGTLVDSVYQHVVAWREALEAIGIELSIWRIHRRIGMSGGLFLAGLLRETGRAVTAEEIRRVQQVHA
ETYGRLLGQVRPLPGARELLDELTRLGIPWAIATSGRLESARKTVAVLGLDADQIPLVTRDHVRHAKPDPDLFLAAAERL
GVEIGESVVVGDSVWDMLAARRARALSVGLLSGGYGAGELLEAGAYRVYDDPADLLRHLDEVGVRAADYEWTLAEPS
>Mature_236_residues
GDDRRMAFLFDLDGTLVDSVYQHVVAWREALEAIGIELSIWRIHRRIGMSGGLFLAGLLRETGRAVTAEEIRRVQQVHAE
TYGRLLGQVRPLPGARELLDELTRLGIPWAIATSGRLESARKTVAVLGLDADQIPLVTRDHVRHAKPDPDLFLAAAERLG
VEIGESVVVGDSVWDMLAARRARALSVGLLSGGYGAGELLEAGAYRVYDDPADLLRHLDEVGVRAADYEWTLAEPS

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrate glucose 6-phosphate [H]

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI87082080, Length=223, Percent_Identity=36.322869955157, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1789046, Length=195, Percent_Identity=32.3076923076923, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 25998; Mature: 25867

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDDRRMAFLFDLDGTLVDSVYQHVVAWREALEAIGIELSIWRIHRRIGMSGGLFLAGLL
CCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHH
RETGRAVTAEEIRRVQQVHAETYGRLLGQVRPLPGARELLDELTRLGIPWAIATSGRLES
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCHHH
ARKTVAVLGLDADQIPLVTRDHVRHAKPDPDLFLAAAERLGVEIGESVVVGDSVWDMLAA
HHHEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCHHHCCEEEECHHHHHHHHH
RRARALSVGLLSGGYGAGELLEAGAYRVYDDPADLLRHLDEVGVRAADYEWTLAEPS
HHHHHHHEEEECCCCCHHHHHHCCCCEEECCHHHHHHHHHHHCCEECCCEEEECCCC
>Mature Secondary Structure 
GDDRRMAFLFDLDGTLVDSVYQHVVAWREALEAIGIELSIWRIHRRIGMSGGLFLAGLL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHH
RETGRAVTAEEIRRVQQVHAETYGRLLGQVRPLPGARELLDELTRLGIPWAIATSGRLES
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCEEEECCCCHHH
ARKTVAVLGLDADQIPLVTRDHVRHAKPDPDLFLAAAERLGVEIGESVVVGDSVWDMLAA
HHHEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHCHHHCCEEEECHHHHHHHHH
RRARALSVGLLSGGYGAGELLEAGAYRVYDDPADLLRHLDEVGVRAADYEWTLAEPS
HHHHHHHEEEECCCCCHHHHHHCCCCEEECCHHHHHHHHHHHCCEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503; 10493123 [H]