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The map label for this gene is cofE [H]

Identifier: 221635954

GI number: 221635954

Start: 543092

End: 543844

Strand: Reverse

Name: cofE [H]

Synonym: trd_A0548

Alternate gene names: 221635954

Gene position: 543844-543092 (Counterclockwise)

Preceding gene: 221635955

Following gene: 221635953

Centisome position: 59.26

GC content: 65.34

Gene sequence:

>753_bases
ATGACCGACGAGGTGCGCCTCATCCCGATCCGGAGGATTCCGGAAGCCCGTCCGGGCGACTCGGTCGCTGAGCTCGTCCT
CACCGGCATGGCAGCCTGTGGCCTCACTGGCGAAGAGGGAGATGTCTTGGTGGTCACCCAGAAACTCGTGTCCAAAGCCG
AGGGGCGGGTAGTGGCACTGGCCGGGATCGAGCCCTCGCCGTTCGCGATCGCCTATGGGCAACGTTGGGAGCGCGATCCG
CGCCAGATCGAGCTGGTGCTTCGCGAAAGCGCACGGATCGTCCGGATGGATCGTGGCTTGATCATCGCCGAGACCCGCCA
CGGGTTCGTCTGCGCCAATGCTGGGGTCGATCTCTCCAATGTCCCTGCCGGCTATGCTGCACTGCTCCCGCTCGATCCGG
ATCGCTCGGCCGCTCAGATCCGAGAGGAACTCGGGGAACGAGTCGGCTGGACCCCGGCTGTGATCGTATCCGATACCTTC
GGTCGGGCCTGGCGCAACGGGATCGTGAACGTCGCGATCGGCGTGGCAGGCATGCGACCCCTACGCGACTATCGGGGACA
ACGCGACCCCTTCGGCAACGAACTCCGGGTCACGGTGATCGCGATCGCCGATGAACTGGCCGCAGCAGCTGAGCTGGTCA
TGGGAAAGACCGAAGGGTGTCCGGCAGTCATCGTGCGGGGTTTCCGGTACGAACCTGGGGAGGGAAGCGCCCGCGAGCTT
CTGATGCCAGCCGAGCGCGACCTTTTCCGCTGA

Upstream 100 bases:

>100_bases
AGCTCGTTCCTGGATCCCACAGTTCCGGGCACAACGACGGGTGACGCTCTTGTTGCGGATCGAGCGGGTGCTCGCGCATG
GCTTTGCGGGAGCCTGAACG

Downstream 100 bases:

>100_bases
GCTTGCTGCTGGGACGCTGAGGACTTAGAATCCTTTCGAGTTCGGTTATTCTGCCAAGTGAAAGGGGGACCAGGATGCCG
GTCCGGACGGCCACGGCGAC

Product: F420-0:gamma-glutamyl ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTDEVRLIPIRRIPEARPGDSVAELVLTGMAACGLTGEEGDVLVVTQKLVSKAEGRVVALAGIEPSPFAIAYGQRWERDP
RQIELVLRESARIVRMDRGLIIAETRHGFVCANAGVDLSNVPAGYAALLPLDPDRSAAQIREELGERVGWTPAVIVSDTF
GRAWRNGIVNVAIGVAGMRPLRDYRGQRDPFGNELRVTVIAIADELAAAAELVMGKTEGCPAVIVRGFRYEPGEGSAREL
LMPAERDLFR

Sequences:

>Translated_250_residues
MTDEVRLIPIRRIPEARPGDSVAELVLTGMAACGLTGEEGDVLVVTQKLVSKAEGRVVALAGIEPSPFAIAYGQRWERDP
RQIELVLRESARIVRMDRGLIIAETRHGFVCANAGVDLSNVPAGYAALLPLDPDRSAAQIREELGERVGWTPAVIVSDTF
GRAWRNGIVNVAIGVAGMRPLRDYRGQRDPFGNELRVTVIAIADELAAAAELVMGKTEGCPAVIVRGFRYEPGEGSAREL
LMPAERDLFR
>Mature_249_residues
TDEVRLIPIRRIPEARPGDSVAELVLTGMAACGLTGEEGDVLVVTQKLVSKAEGRVVALAGIEPSPFAIAYGQRWERDPR
QIELVLRESARIVRMDRGLIIAETRHGFVCANAGVDLSNVPAGYAALLPLDPDRSAAQIREELGERVGWTPAVIVSDTFG
RAWRNGIVNVAIGVAGMRPLRDYRGQRDPFGNELRVTVIAIADELAAAAELVMGKTEGCPAVIVRGFRYEPGEGSARELL
MPAERDLFR

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives [H]

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002847
- InterPro:   IPR008225 [H]

Pfam domain/function: PF01996 F420_ligase [H]

EC number: NA

Molecular weight: Translated: 27124; Mature: 26992

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDEVRLIPIRRIPEARPGDSVAELVLTGMAACGLTGEEGDVLVVTQKLVSKAEGRVVAL
CCCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCCCEEEE
AGIEPSPFAIAYGQRWERDPRQIELVLRESARIVRMDRGLIIAETRHGFVCANAGVDLSN
ECCCCCCEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCEEEEECCCCEEEECCCCCCCC
VPAGYAALLPLDPDRSAAQIREELGERVGWTPAVIVSDTFGRAWRNGIVNVAIGVAGMRP
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCEEEEEECCCCCH
LRDYRGQRDPFGNELRVTVIAIADELAAAAELVMGKTEGCPAVIVRGFRYEPGEGSAREL
HHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCHHHEEECEEECCCCCCHHHC
LMPAERDLFR
CCCCHHHHCC
>Mature Secondary Structure 
TDEVRLIPIRRIPEARPGDSVAELVLTGMAACGLTGEEGDVLVVTQKLVSKAEGRVVAL
CCCEEEEEHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEHHHHHHCCCCEEEE
AGIEPSPFAIAYGQRWERDPRQIELVLRESARIVRMDRGLIIAETRHGFVCANAGVDLSN
ECCCCCCEEEECCCCCCCCHHHHHHHHHHCCEEEEECCCEEEEECCCCEEEECCCCCCCC
VPAGYAALLPLDPDRSAAQIREELGERVGWTPAVIVSDTFGRAWRNGIVNVAIGVAGMRP
CCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECCHHHHHHCCCEEEEEECCCCCH
LRDYRGQRDPFGNELRVTVIAIADELAAAAELVMGKTEGCPAVIVRGFRYEPGEGSAREL
HHHHCCCCCCCCCCEEEEEEEEHHHHHHHHHHHHCCCCCCHHHEEECEEECCCCCCHHHC
LMPAERDLFR
CCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014 [H]