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The map label for this gene is lpdA [H]

Identifier: 221635892

GI number: 221635892

Start: 474743

End: 476152

Strand: Reverse

Name: lpdA [H]

Synonym: trd_A0486

Alternate gene names: 221635892

Gene position: 476152-474743 (Counterclockwise)

Preceding gene: 221635893

Following gene: 221635891

Centisome position: 51.88

GC content: 64.82

Gene sequence:

>1410_bases
ATGACCGTCGAACAGCGCCAGGAGTTCGACGTCGTATTCCTCGGTGGAGGGACCGGCGGCTACGTCGCAGCGATTCGCGC
TGCCCAACTCGGCCTGAAGGTCGCTGTCGTCGAGAAGGACAAGGTCGGCGGGACGTGCTTGCACCGGGGCTGCATTCCGA
GCAAGGCGCTCCTCAAGAGCGCGGAACTCCTCGAGCAAGCGCGTCGGGCCAAGGAGTTCGGCGTCATCGTCGGTGAGGTC
GCGGGCGATTATCCGACCGCCTTCCGGCGCGCCCAGCAGGTGGTCGAGCAGCTCCACAAGGGGATCCACTTCCTCTTCCG
CAAGCACGGCATCACGCTCATCCAGGGAGTCGGCCGGCTGACCCGGAATCGCACTGTCCTGGTCAACGGTGCCGAGGGCC
AGCCCCAGGAGCTGCGTGGGCGTGCGATCGTGATCGACACTGGTTCTCGGCCACGAGCGATCCCTGGCATCCCATTCGAC
GGCGTCCGCGTGCTCAACAGCGACCACACCACGGCACAGATCGACTGGTACCCCAAGCGCGTCATCATCCGCGGGGGCGG
CGCAACGGGTGTCGAGCATGCGACGGTCTGGCACGCCTTCGGGGCTGAGGTCACGCTGGTGGGACGTATCGTCCCCAACG
AGGACGAGGAAGTCCAACAACAGCTCGTCCGAGCCTTCCAGCGCAAGGGGATCCGCATCGTTCCGGACTACCGCCCGACC
GCCGACGACTTCGATATCACCGAGGGCGGTGTCCGGATGCGCGTACGCAAGAGTGGTACGCAAGAAGAGGTCATCGAGGC
CGATGCGCTCTTCGTCGCGCTCGGCCGCGAAGGGAACATCGAGGAAATCGGGCTCGAGGAGCTGGGGGTCCGCACCAGGG
ACGGCTTCATCGTCACCGACGAGTATTTCCGCACGAACGTCGAGGGTATTTACGCCATCGGCGATGTGCTCGGTATCCAG
CAACTGGCGCACACCGCGATGCACCAGGGGATCATCGCCGTCGAGCATATCGCCGGCGAGAAACCGCTCCCCCTCGATTA
TCACCGTGTCCCGATCGTGACCTACTGCCATCCGGAGATCGCGAGCCTCGGCCTGACCGAGCGGGAAGCCAAGGAGCAGG
GTCGGGCGATCAAAGTCGGCAAGTTCCCCTTCCGAGCCAACGGAAAATCGCTCATCGAGGGGGAGACCGACGGCTTCGTC
AAGATCATCGCCGATGCCGAAACGAACGATATCCTCGGCGTCCATATCATCGGCAATCACGCCACCGAGCTGATCGCCGA
GGCCGCCTTGGCCAAACTGCTCGAGGCCACGCCCTGGGAGATCGGGCTCTCCGTTCACCCGCACCCGACCGTCTCCGAGG
TGATCGGCGAGGCCGCGCTGGCGGTCGACAACCTGGCCATCCACATCTGA

Upstream 100 bases:

>100_bases
ACATCGCGGTACGCCCGGGCTTCCCTGCTATACTGCCCACAGACAGCCTCCGGTTTGCCGGAGGAGGGTCTTCATCCGTC
GCGAACCGAAGGAGGACGGG

Downstream 100 bases:

>100_bases
CGCACGCCTACTCGACTCGTGGCGAGCTGGCGATAAATGAGCGCGTGAAGGGGATTCGATCACCATGTTCGGGGAGCGAC
ATCGATGACCGCGATCGTCG

Product: dihydrolipoyl dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of branched-chain alpha-keto acid dehydrogenase complex; LPD-Val [H]

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTVEQRQEFDVVFLGGGTGGYVAAIRAAQLGLKVAVVEKDKVGGTCLHRGCIPSKALLKSAELLEQARRAKEFGVIVGEV
AGDYPTAFRRAQQVVEQLHKGIHFLFRKHGITLIQGVGRLTRNRTVLVNGAEGQPQELRGRAIVIDTGSRPRAIPGIPFD
GVRVLNSDHTTAQIDWYPKRVIIRGGGATGVEHATVWHAFGAEVTLVGRIVPNEDEEVQQQLVRAFQRKGIRIVPDYRPT
ADDFDITEGGVRMRVRKSGTQEEVIEADALFVALGREGNIEEIGLEELGVRTRDGFIVTDEYFRTNVEGIYAIGDVLGIQ
QLAHTAMHQGIIAVEHIAGEKPLPLDYHRVPIVTYCHPEIASLGLTEREAKEQGRAIKVGKFPFRANGKSLIEGETDGFV
KIIADAETNDILGVHIIGNHATELIAEAALAKLLEATPWEIGLSVHPHPTVSEVIGEAALAVDNLAIHI

Sequences:

>Translated_469_residues
MTVEQRQEFDVVFLGGGTGGYVAAIRAAQLGLKVAVVEKDKVGGTCLHRGCIPSKALLKSAELLEQARRAKEFGVIVGEV
AGDYPTAFRRAQQVVEQLHKGIHFLFRKHGITLIQGVGRLTRNRTVLVNGAEGQPQELRGRAIVIDTGSRPRAIPGIPFD
GVRVLNSDHTTAQIDWYPKRVIIRGGGATGVEHATVWHAFGAEVTLVGRIVPNEDEEVQQQLVRAFQRKGIRIVPDYRPT
ADDFDITEGGVRMRVRKSGTQEEVIEADALFVALGREGNIEEIGLEELGVRTRDGFIVTDEYFRTNVEGIYAIGDVLGIQ
QLAHTAMHQGIIAVEHIAGEKPLPLDYHRVPIVTYCHPEIASLGLTEREAKEQGRAIKVGKFPFRANGKSLIEGETDGFV
KIIADAETNDILGVHIIGNHATELIAEAALAKLLEATPWEIGLSVHPHPTVSEVIGEAALAVDNLAIHI
>Mature_468_residues
TVEQRQEFDVVFLGGGTGGYVAAIRAAQLGLKVAVVEKDKVGGTCLHRGCIPSKALLKSAELLEQARRAKEFGVIVGEVA
GDYPTAFRRAQQVVEQLHKGIHFLFRKHGITLIQGVGRLTRNRTVLVNGAEGQPQELRGRAIVIDTGSRPRAIPGIPFDG
VRVLNSDHTTAQIDWYPKRVIIRGGGATGVEHATVWHAFGAEVTLVGRIVPNEDEEVQQQLVRAFQRKGIRIVPDYRPTA
DDFDITEGGVRMRVRKSGTQEEVIEADALFVALGREGNIEEIGLEELGVRTRDGFIVTDEYFRTNVEGIYAIGDVLGIQQ
LAHTAMHQGIIAVEHIAGEKPLPLDYHRVPIVTYCHPEIASLGLTEREAKEQGRAIKVGKFPFRANGKSLIEGETDGFVK
IIADAETNDILGVHIIGNHATELIAEAALAKLLEATPWEIGLSVHPHPTVSEVIGEAALAVDNLAIHI

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=468, Percent_Identity=37.8205128205128, Blast_Score=278, Evalue=8e-75,
Organism=Homo sapiens, GI50301238, Length=460, Percent_Identity=27.3913043478261, Blast_Score=145, Evalue=8e-35,
Organism=Homo sapiens, GI33519430, Length=462, Percent_Identity=26.4069264069264, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI33519428, Length=462, Percent_Identity=26.4069264069264, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI33519426, Length=462, Percent_Identity=26.4069264069264, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI148277071, Length=462, Percent_Identity=26.4069264069264, Blast_Score=129, Evalue=8e-30,
Organism=Homo sapiens, GI148277065, Length=462, Percent_Identity=26.4069264069264, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI291045266, Length=455, Percent_Identity=25.0549450549451, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI22035672, Length=461, Percent_Identity=26.8980477223427, Blast_Score=105, Evalue=9e-23,
Organism=Homo sapiens, GI291045268, Length=452, Percent_Identity=24.3362831858407, Blast_Score=103, Evalue=4e-22,
Organism=Escherichia coli, GI1786307, Length=464, Percent_Identity=34.698275862069, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI87082354, Length=426, Percent_Identity=28.4037558685446, Blast_Score=167, Evalue=1e-42,
Organism=Escherichia coli, GI1789915, Length=438, Percent_Identity=30.365296803653, Blast_Score=167, Evalue=2e-42,
Organism=Escherichia coli, GI87081717, Length=468, Percent_Identity=26.7094017094017, Blast_Score=137, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI32565766, Length=464, Percent_Identity=37.2844827586207, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI17557007, Length=478, Percent_Identity=24.8953974895398, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=475, Percent_Identity=26.9473684210526, Blast_Score=111, Evalue=7e-25,
Organism=Caenorhabditis elegans, GI71983419, Length=475, Percent_Identity=26.7368421052632, Blast_Score=111, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI71982272, Length=480, Percent_Identity=25.2083333333333, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321091, Length=484, Percent_Identity=37.8099173553719, Blast_Score=260, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=26.6666666666667, Blast_Score=157, Evalue=2e-39,
Organism=Saccharomyces cerevisiae, GI6325240, Length=479, Percent_Identity=27.7661795407098, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI21358499, Length=466, Percent_Identity=38.412017167382, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24640549, Length=474, Percent_Identity=26.1603375527426, Blast_Score=125, Evalue=8e-29,
Organism=Drosophila melanogaster, GI24640553, Length=469, Percent_Identity=26.226012793177, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640551, Length=469, Percent_Identity=26.226012793177, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17737741, Length=471, Percent_Identity=27.1762208067941, Blast_Score=121, Evalue=9e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51075; Mature: 50944

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVEQRQEFDVVFLGGGTGGYVAAIRAAQLGLKVAVVEKDKVGGTCLHRGCIPSKALLKS
CCCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCCHHHHHHH
AELLEQARRAKEFGVIVGEVAGDYPTAFRRAQQVVEQLHKGIHFLFRKHGITLIQGVGRL
HHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TRNRTVLVNGAEGQPQELRGRAIVIDTGSRPRAIPGIPFDGVRVLNSDHTTAQIDWYPKR
HCCCEEEEECCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEEEECEE
VIIRGGGATGVEHATVWHAFGAEVTLVGRIVPNEDEEVQQQLVRAFQRKGIRIVPDYRPT
EEEECCCCCCCCHHEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCC
ADDFDITEGGVRMRVRKSGTQEEVIEADALFVALGREGNIEEIGLEELGVRTRDGFIVTD
CCCCCCCCCCEEEEEECCCCHHHHHHHCEEEEEECCCCCHHHCCHHHCCCEECCCEEEEH
EYFRTNVEGIYAIGDVLGIQQLAHTAMHQGIIAVEHIAGEKPLPLDYHRVPIVTYCHPEI
HHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCHHH
ASLGLTEREAKEQGRAIKVGKFPFRANGKSLIEGETDGFVKIIADAETNDILGVHIIGNH
HHCCCCHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCCEEEEEEECCH
ATELIAEAALAKLLEATPWEIGLSVHPHPTVSEVIGEAALAVDNLAIHI
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEC
>Mature Secondary Structure 
TVEQRQEFDVVFLGGGTGGYVAAIRAAQLGLKVAVVEKDKVGGTCLHRGCIPSKALLKS
CCCCCCCCCEEEEECCCCCHHHHHHHHHCCEEEEEEECCCCCCCEEECCCCCHHHHHHH
AELLEQARRAKEFGVIVGEVAGDYPTAFRRAQQVVEQLHKGIHFLFRKHGITLIQGVGRL
HHHHHHHHHHHHHCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TRNRTVLVNGAEGQPQELRGRAIVIDTGSRPRAIPGIPFDGVRVLNSDHTTAQIDWYPKR
HCCCEEEEECCCCCHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCCCCEEEEEEECEE
VIIRGGGATGVEHATVWHAFGAEVTLVGRIVPNEDEEVQQQLVRAFQRKGIRIVPDYRPT
EEEECCCCCCCCHHEEEEECCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCEEECCCCCC
ADDFDITEGGVRMRVRKSGTQEEVIEADALFVALGREGNIEEIGLEELGVRTRDGFIVTD
CCCCCCCCCCEEEEEECCCCHHHHHHHCEEEEEECCCCCHHHCCHHHCCCEECCCEEEEH
EYFRTNVEGIYAIGDVLGIQQLAHTAMHQGIIAVEHIAGEKPLPLDYHRVPIVTYCHPEI
HHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEECCHHH
ASLGLTEREAKEQGRAIKVGKFPFRANGKSLIEGETDGFVKIIADAETNDILGVHIIGNH
HHCCCCHHHHHHCCCEEEEECCCCCCCCCEEECCCCCCEEEEEEECCCCCEEEEEEECCH
ATELIAEAALAKLLEATPWEIGLSVHPHPTVSEVIGEAALAVDNLAIHI
HHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 8504804 [H]