| Definition | Thermomicrobium roseum DSM 5159 plasmid unnamed, complete sequence. |
|---|---|
| Accession | NC_011961 |
| Length | 917,738 |
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The map label for this gene is glnA [H]
Identifier: 221635836
GI number: 221635836
Start: 416018
End: 417403
Strand: Reverse
Name: glnA [H]
Synonym: trd_A0430
Alternate gene names: 221635836
Gene position: 417403-416018 (Counterclockwise)
Preceding gene: 221635840
Following gene: 221635835
Centisome position: 45.48
GC content: 64.79
Gene sequence:
>1386_bases ATGAGCGAGCATACGACGGGTCTCTTGACCCTGGACGAACTGGAGCACCTGGTCGCCAGTGGCGAGATCGATACGGTCAT CAACGCGCTTTGCGACATGCAGGGACGCCTCATGGGGAAGCGCGTCCGCGCCGATTTCTTCCTCGAGCACGTCCGCGCCC ACGGCACCCACTTCTGCACCTACCTCCTCGGTACCGACATGGAGATGACCACACCGGGCGGTTACCGGTTGATGAATTGG GAGACCGGCTACGGTGACTGGGTCGCCCGTCCCGACTTCGCCACGCTGCGGCGTATCCCCTGGCTGGAGGGAACCGCGCT CGTCTTGGCCGACGCGGTCGACGAGGAAACCGGTGAGCTGATCCCGGTCGCCCCCCGGACGATCCTCCGCCGCCAGGTCG AGCGCGCCGCAGCCATGGGCTTCCGCATCATGATGGCGAGCGAGCTGGAGTTCTACCTCCTCAAGGACTCCTATGACGAG GCGTACGAGAAGGGGTACCGTGGTCTGCACCCCTACGGCTGGTACAACGAGGACTATCACCTCTTCCAGGGAACCAAAGC GGAACCGATCTACCGGCAGATCCGCAACCTGATGACGGCTGCTGGGATCCCGATCGAGTTCTCGAAGGGGGAGGCAGCGG CTGGCCAGCACGAGATCAACATCCACTACGCGGAGGCGCTCGAATCGGCCGATCGTGCGGTGCTCTTCAAGCACGGCGTC AAGGAGATCGCCTACCTCAATGGGGTGGCAGTCACCTTCATGGCCAAGCCCCATCACACCTGGACCGGCTCCAGTTCCCA CATCCACTTGAGCATCTGGGACAGCGCGGGCGAGCGGAACCTCTTCTACGAGGCAGACGGGCAACCCTACCACATGAGTA AGCTGATGCAGCACGCACTCGCCGGACTCATCGCCCACGCCCGGGAACTTTCGCTCTTCGTCGCCTCCAACGTCAACTCG TACAAGCGCTACGCCGTCGCTAGCTGGGCACCGGTCAACGTCGTCTGGGGACGCGACAACCGCACCTGTGGCTTTCGCAT CGTCGGCCACGGCCAGTCATTGCGCGTCGAGAGCCGCCTTCCGGGCGCCGACGCCAACCCGTACCTGGCTTATGCCGCGA TGATCGCCGCCGCGCTGGACGGGATCGAGCGAGAACTTCCCCTGCCGCCCGAGTACCGCGGCAACGCCTACGAAGCCCGC GACGTCGTCCGTGTTCCGCGCACCCTCGCCGAGGCGATGGTCGAGTTCGAGCGCAGCGAACTGGCCCGCCGCGCTTTCGG CGAGCTCGTCGTCGAGCACTACGTCAACATGGCGCGGGTCGAGCAGGAGACGTTCGACGCCGTGGTGACCGATTGGGAGC TGTACCGGTACTTCGAGCGCGGCTGA
Upstream 100 bases:
>100_bases CAGTGCTGCTGGTTAGCCGGCGGTAGCCGTCACCCCCCGCCCGCTGACTCACCTCCGGCCGATCGGTGAGGCAACGAGCG AAGGGAGTTGGAGCGCATCG
Downstream 100 bases:
>100_bases GGGAGGGGGTAACGATGGGCAACCGGCTGGCTGGCAAAGTGTGCATCATCACTGGAGCAGGATCGGGAATCGGGCGCGAG GCGGCCATCCTCTTCGCCCA
Product: glutamate--ammonia ligase
Products: NA
Alternate protein names: GS; Glutamate--ammonia ligase [H]
Number of amino acids: Translated: 461; Mature: 460
Protein sequence:
>461_residues MSEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCTYLLGTDMEMTTPGGYRLMNW ETGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGELIPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDE AYEKGYRGLHPYGWYNEDYHLFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGV KEIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHALAGLIAHARELSLFVASNVNS YKRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRLPGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEAR DVVRVPRTLAEAMVEFERSELARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG
Sequences:
>Translated_461_residues MSEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCTYLLGTDMEMTTPGGYRLMNW ETGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGELIPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDE AYEKGYRGLHPYGWYNEDYHLFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGV KEIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHALAGLIAHARELSLFVASNVNS YKRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRLPGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEAR DVVRVPRTLAEAMVEFERSELARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG >Mature_460_residues SEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCTYLLGTDMEMTTPGGYRLMNWE TGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGELIPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDEA YEKGYRGLHPYGWYNEDYHLFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGVK EIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHALAGLIAHARELSLFVASNVNSY KRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRLPGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEARD VVRVPRTLAEAMVEFERSELARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG
Specific function: Unknown
COG id: COG0174
COG function: function code E; Glutamine synthetase
Gene ontology:
Cell location: Cytoplasm (Potential) [H]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the glutamine synthetase family [H]
Homologues:
Organism=Homo sapiens, GI221307485, Length=435, Percent_Identity=27.3563218390805, Blast_Score=147, Evalue=2e-35, Organism=Escherichia coli, GI87081870, Length=439, Percent_Identity=31.4350797266515, Blast_Score=167, Evalue=1e-42, Organism=Escherichia coli, GI1790301, Length=437, Percent_Identity=26.3157894736842, Blast_Score=124, Evalue=1e-29,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR008147 - InterPro: IPR014746 - InterPro: IPR008146 - InterPro: IPR004809 [H]
Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]
EC number: =6.3.1.2 [H]
Molecular weight: Translated: 52177; Mature: 52046
Theoretical pI: Translated: 5.79; Mature: 5.79
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.7 %Cys (Translated Protein) 3.5 %Met (Translated Protein) 4.1 %Cys+Met (Translated Protein) 0.7 %Cys (Mature Protein) 3.3 %Met (Mature Protein) 3.9 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCT CCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE YLLGTDMEMTTPGGYRLMNWETGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGEL HHHCCCCEEECCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCE IPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDEAYEKGYRGLHPYGWYNEDYH EECCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEE LFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGV EECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHH KEIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHAL HHHHHHCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHH AGLIAHARELSLFVASNVNSYKRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRL HHHHHHHHHHEEEEECCCCHHHHEEEECCCCEEEEECCCCCCEEEEEEECCCEEEECCCC PGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEARDVVRVPRTLAEAMVEFERSE CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH LARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC >Mature Secondary Structure SEHTTGLLTLDELEHLVASGEIDTVINALCDMQGRLMGKRVRADFFLEHVRAHGTHFCT CCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEE YLLGTDMEMTTPGGYRLMNWETGYGDWVARPDFATLRRIPWLEGTALVLADAVDEETGEL HHHCCCCEEECCCCEEEEEEECCCCCCCCCCCHHHHHHCCCCCCCCEEEEECCCCCCCCE IPVAPRTILRRQVERAAAMGFRIMMASELEFYLLKDSYDEAYEKGYRGLHPYGWYNEDYH EECCHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCEE LFQGTKAEPIYRQIRNLMTAAGIPIEFSKGEAAAGQHEINIHYAEALESADRAVLFKHGV EECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCCEEEEEEHHHHHCCCCHHHHHHHHH KEIAYLNGVAVTFMAKPHHTWTGSSSHIHLSIWDSAGERNLFYEADGQPYHMSKLMQHAL HHHHHHCCEEEEEEECCCCCCCCCCCEEEEEEECCCCCCCEEEECCCCCHHHHHHHHHHH AGLIAHARELSLFVASNVNSYKRYAVASWAPVNVVWGRDNRTCGFRIVGHGQSLRVESRL HHHHHHHHHHEEEEECCCCHHHHEEEECCCCEEEEECCCCCCEEEEEEECCCEEEECCCC PGADANPYLAYAAMIAAALDGIERELPLPPEYRGNAYEARDVVRVPRTLAEAMVEFERSE CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH LARRAFGELVVEHYVNMARVEQETFDAVVTDWELYRYFERG HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8688087 [H]