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The map label for this gene is paaF [C]

Identifier: 221635739

GI number: 221635739

Start: 320883

End: 321656

Strand: Reverse

Name: paaF [C]

Synonym: trd_A0333

Alternate gene names: 221635739

Gene position: 321656-320883 (Counterclockwise)

Preceding gene: 221635740

Following gene: 221635738

Centisome position: 35.05

GC content: 57.75

Gene sequence:

>774_bases
ATGAGTGATCTGCTGGTCACGCTCCAGACTTTGGACGACACAGCGATCGTCACGCTCAATCGTCCAGAAAAGCTCAACGC
TCTCAATCTCGAACTCGTCAGTGCGCTCCGGGACACGCTGCTTGCGGTGGCCAACGATGCTCGTCTCCGTCTCGTCATCA
TCCGCGGAGCAGGACGGAGCTTCTGCTCTGGTTTGGATCTCGACATGATGGCAGCCGAAGGAATGCCGCCCGAATTTTAT
CCTGTTCAGGAAGAAGCTTTCCGACTTATCGAAACGATGAACAAACTCGTTGTCGCGGCGATCCACGGATATTGCTTGGG
AGGAGGAGTCCAACTTGCCATCGCCTGTGATTTGCGTATCGCCAGTGACGACACGGTGCTCGCACTCCCGGCGATCAACG
AAGGACTCTTCCCCGGCATGGCACCCTACCGCCTCCCGCGATTGATCGGGCTGGGGCTCGCACGTCGGTACATCTATACG
GGGGATCCTATTCCGCTCGACCTCGCGCACCAGATCGGCCTCATCGACTGGATCATCCCCGCTCGATCCTTCGAGGATGA
ACTCGAAAAGCTCCTTCAAAAGCTCCGCGCTGTACCGTTCACCGCAGCCATCCATGGGAAACAACTGATGGCGAACGCTT
TCGATCAATCGTTCAGCGCGGCGTATCACGACTCTCTTTGGCGTCTCGAACAGTGCCTCAGTTCGGACGACGTCGCCCGT
GCTCGTGCCTTGTGGATGGAACGGAAGCAAAGCCGAACCGCCCAACGCCCATAG

Upstream 100 bases:

>100_bases
CGAGTGAAAGTGCGAGCACACCCCAGGCGAACCGCCTCGCCTATGCCGCTTTGCTGGAAACCCTTGCCTCCCGAGTGTAG
TCAGCCTGAGGAGGAGACCG

Downstream 100 bases:

>100_bases
GAGGGATCGATGGTGATCGGGTCATCCACTCGCCGTCTCGTCGGGAAGCAAGCAGAGTCCAGTCTTACGGGGTTCGGCCT
CCTGCGGCGCTTTGCACCGA

Product: 3-hydroxyacyl-CoA dehydrogenase NAD-binding

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 257; Mature: 256

Protein sequence:

>257_residues
MSDLLVTLQTLDDTAIVTLNRPEKLNALNLELVSALRDTLLAVANDARLRLVIIRGAGRSFCSGLDLDMMAAEGMPPEFY
PVQEEAFRLIETMNKLVVAAIHGYCLGGGVQLAIACDLRIASDDTVLALPAINEGLFPGMAPYRLPRLIGLGLARRYIYT
GDPIPLDLAHQIGLIDWIIPARSFEDELEKLLQKLRAVPFTAAIHGKQLMANAFDQSFSAAYHDSLWRLEQCLSSDDVAR
ARALWMERKQSRTAQRP

Sequences:

>Translated_257_residues
MSDLLVTLQTLDDTAIVTLNRPEKLNALNLELVSALRDTLLAVANDARLRLVIIRGAGRSFCSGLDLDMMAAEGMPPEFY
PVQEEAFRLIETMNKLVVAAIHGYCLGGGVQLAIACDLRIASDDTVLALPAINEGLFPGMAPYRLPRLIGLGLARRYIYT
GDPIPLDLAHQIGLIDWIIPARSFEDELEKLLQKLRAVPFTAAIHGKQLMANAFDQSFSAAYHDSLWRLEQCLSSDDVAR
ARALWMERKQSRTAQRP
>Mature_256_residues
SDLLVTLQTLDDTAIVTLNRPEKLNALNLELVSALRDTLLAVANDARLRLVIIRGAGRSFCSGLDLDMMAAEGMPPEFYP
VQEEAFRLIETMNKLVVAAIHGYCLGGGVQLAIACDLRIASDDTVLALPAINEGLFPGMAPYRLPRLIGLGLARRYIYTG
DPIPLDLAHQIGLIDWIIPARSFEDELEKLLQKLRAVPFTAAIHGKQLMANAFDQSFSAAYHDSLWRLEQCLSSDDVARA
RALWMERKQSRTAQRP

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI20127408, Length=175, Percent_Identity=36.5714285714286, Blast_Score=100, Evalue=1e-21,
Organism=Homo sapiens, GI70995211, Length=181, Percent_Identity=33.1491712707182, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI194097323, Length=241, Percent_Identity=25.3112033195021, Blast_Score=95, Evalue=6e-20,
Organism=Homo sapiens, GI31542718, Length=225, Percent_Identity=27.5555555555556, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI37594471, Length=152, Percent_Identity=34.8684210526316, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI37594469, Length=152, Percent_Identity=34.8684210526316, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI68989263, Length=175, Percent_Identity=30.8571428571429, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI62530384, Length=245, Percent_Identity=27.3469387755102, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI213417737, Length=204, Percent_Identity=25.9803921568627, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI157694516, Length=204, Percent_Identity=25.9803921568627, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI295842266, Length=241, Percent_Identity=26.5560165975104, Blast_Score=69, Evalue=5e-12,
Organism=Escherichia coli, GI1787659, Length=182, Percent_Identity=34.0659340659341, Blast_Score=110, Evalue=8e-26,
Organism=Escherichia coli, GI1788682, Length=178, Percent_Identity=36.5168539325843, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI221142681, Length=183, Percent_Identity=32.2404371584699, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1790281, Length=201, Percent_Identity=27.8606965174129, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788597, Length=194, Percent_Identity=28.8659793814433, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI87082183, Length=191, Percent_Identity=24.6073298429319, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787660, Length=190, Percent_Identity=29.4736842105263, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17534483, Length=263, Percent_Identity=28.8973384030418, Blast_Score=106, Evalue=9e-24,
Organism=Caenorhabditis elegans, GI25145438, Length=220, Percent_Identity=29.0909090909091, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17554946, Length=184, Percent_Identity=30.9782608695652, Blast_Score=98, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17540714, Length=182, Percent_Identity=31.3186813186813, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=26.4150943396226, Blast_Score=94, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI25144276, Length=176, Percent_Identity=38.6363636363636, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=176, Percent_Identity=38.6363636363636, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=176, Percent_Identity=38.6363636363636, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558304, Length=175, Percent_Identity=33.1428571428571, Blast_Score=88, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI25144160, Length=165, Percent_Identity=36.969696969697, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI25144157, Length=165, Percent_Identity=36.969696969697, Blast_Score=87, Evalue=9e-18,
Organism=Caenorhabditis elegans, GI17549921, Length=220, Percent_Identity=27.2727272727273, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17560910, Length=177, Percent_Identity=29.3785310734463, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI25141363, Length=184, Percent_Identity=25.5434782608696, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=193, Percent_Identity=25.9067357512953, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=216, Percent_Identity=32.4074074074074, Blast_Score=92, Evalue=6e-20,
Organism=Drosophila melanogaster, GI20129971, Length=189, Percent_Identity=30.6878306878307, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24653477, Length=189, Percent_Identity=30.6878306878307, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI21357171, Length=233, Percent_Identity=28.3261802575107, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24653139, Length=178, Percent_Identity=30.8988764044944, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI19922422, Length=252, Percent_Identity=21.8253968253968, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571730, Length=147, Percent_Identity=37.4149659863946, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI28571729, Length=147, Percent_Identity=37.4149659863946, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24654903, Length=240, Percent_Identity=24.1666666666667, Blast_Score=79, Evalue=3e-15,
Organism=Drosophila melanogaster, GI19920382, Length=182, Percent_Identity=26.3736263736264, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24650670, Length=229, Percent_Identity=24.0174672489083, Blast_Score=71, Evalue=7e-13,
Organism=Drosophila melanogaster, GI19921000, Length=179, Percent_Identity=30.7262569832402, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24583077, Length=179, Percent_Identity=30.7262569832402, Blast_Score=68, Evalue=8e-12,
Organism=Drosophila melanogaster, GI24583079, Length=179, Percent_Identity=30.7262569832402, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 28442; Mature: 28311

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDLLVTLQTLDDTAIVTLNRPEKLNALNLELVSALRDTLLAVANDARLRLVIIRGAGRS
CCCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHH
FCSGLDLDMMAAEGMPPEFYPVQEEAFRLIETMNKLVVAAIHGYCLGGGVQLAIACDLRI
HHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEEEEEE
ASDDTVLALPAINEGLFPGMAPYRLPRLIGLGLARRYIYTGDPIPLDLAHQIGLIDWIIP
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHCCHHHHHHC
ARSFEDELEKLLQKLRAVPFTAAIHGKQLMANAFDQSFSAAYHDSLWRLEQCLSSDDVAR
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ARALWMERKQSRTAQRP
HHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDLLVTLQTLDDTAIVTLNRPEKLNALNLELVSALRDTLLAVANDARLRLVIIRGAGRS
CCCEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCHH
FCSGLDLDMMAAEGMPPEFYPVQEEAFRLIETMNKLVVAAIHGYCLGGGVQLAIACDLRI
HHCCCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCEEECCCEEEEEEEEEEE
ASDDTVLALPAINEGLFPGMAPYRLPRLIGLGLARRYIYTGDPIPLDLAHQIGLIDWIIP
CCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHEEECCCCCCHHHHHHCCHHHHHHC
ARSFEDELEKLLQKLRAVPFTAAIHGKQLMANAFDQSFSAAYHDSLWRLEQCLSSDDVAR
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ARALWMERKQSRTAQRP
HHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA