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The map label for this gene is lutR [H]

Identifier: 221635559

GI number: 221635559

Start: 142822

End: 143601

Strand: Reverse

Name: lutR [H]

Synonym: trd_A0153

Alternate gene names: 221635559

Gene position: 143601-142822 (Counterclockwise)

Preceding gene: 221635560

Following gene: 221635555

Centisome position: 15.65

GC content: 66.79

Gene sequence:

>780_bases
ATGACGAGAGCGATACCGGTCACTGCGAAGCCCATCACCCAGGCACGACTCCACCAGCGAGTTGTCGAGGAACTTCTGCG
GCAAATCGTCAGTGGCGCATTGCCGCCCGGCACCACGCTGCCGTCCGAGCCGGAACTGGCCCGGCAATTCGGGGTGAGTC
GCATCGTCATTCGGGAGGCGATCCGTATCCTCGTCGAGAAGGGCCTGATCACGGTCCGACATGGCAGCGGGATGTGGGTC
CAGTCGATCGAGCAGTGGAACCATCTCGACCCGATGGTCCTCCTCGAGGAAGTCCGCTCGAGCCCGGACGCGAGCCGGCT
CAGTGAACTCCTCGAGCTCTGCGAGATCCTCGACGTCGGTGCCGCCGAGCGGGCCGCTACCCGCCGCACCCCCGAGCAGC
TGTCTCGGTTGCGCGACCTGGTCGAGCGGATGCGCAAGGTGGTCGATGATCCGGCAGCCTACCTCGATCTGGATGCGCTT
TTCCATGCGACGATCGCTGAGAGTGCCGGAAACCGCCTCCTCCGCGATGCGCAGCGACCGCTCAGCGAGGTGCTGTTCGC
CAACTGGCTGTGGGCCACACGGGCACCGGAGGAGCTCACCCGCTTCCAGCAAGACCACGAGGCGATCTATGAGGCGATCG
CCGCCGGTGATCCACTCGGCGCTCGCCAAGCGATGCACCGGCACGCCCAGCATGTGGCCAGCGCTCTCCGCGCCGGAATC
GCCCCAGCGGCGGGGGAACTGGTCACGACGGGCAGCGACGGTCGCCACGCTGCTGCTTGA

Upstream 100 bases:

>100_bases
TATCTGAGTGGCGGGCATCACCGCCGCGTTCGTCGAAGTGCAACGGGTAAACTTTAGAAGCGAACATATTGCGTCGGAGA
AACACATAAAGGAGAGGATC

Downstream 100 bases:

>100_bases
GCGAGTCCACGAGAGCGGGACGGGTATTCCGTCCCGCTCTTTTTTTCAGCGCCATCACTGGACAGGAGCGAGACTGTGCC
GGACAGCGCGCCCGGTCAGA

Product: transcriptional regulator, GntR family

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 259; Mature: 258

Protein sequence:

>259_residues
MTRAIPVTAKPITQARLHQRVVEELLRQIVSGALPPGTTLPSEPELARQFGVSRIVIREAIRILVEKGLITVRHGSGMWV
QSIEQWNHLDPMVLLEEVRSSPDASRLSELLELCEILDVGAAERAATRRTPEQLSRLRDLVERMRKVVDDPAAYLDLDAL
FHATIAESAGNRLLRDAQRPLSEVLFANWLWATRAPEELTRFQQDHEAIYEAIAAGDPLGARQAMHRHAQHVASALRAGI
APAAGELVTTGSDGRHAAA

Sequences:

>Translated_259_residues
MTRAIPVTAKPITQARLHQRVVEELLRQIVSGALPPGTTLPSEPELARQFGVSRIVIREAIRILVEKGLITVRHGSGMWV
QSIEQWNHLDPMVLLEEVRSSPDASRLSELLELCEILDVGAAERAATRRTPEQLSRLRDLVERMRKVVDDPAAYLDLDAL
FHATIAESAGNRLLRDAQRPLSEVLFANWLWATRAPEELTRFQQDHEAIYEAIAAGDPLGARQAMHRHAQHVASALRAGI
APAAGELVTTGSDGRHAAA
>Mature_258_residues
TRAIPVTAKPITQARLHQRVVEELLRQIVSGALPPGTTLPSEPELARQFGVSRIVIREAIRILVEKGLITVRHGSGMWVQ
SIEQWNHLDPMVLLEEVRSSPDASRLSELLELCEILDVGAAERAATRRTPEQLSRLRDLVERMRKVVDDPAAYLDLDALF
HATIAESAGNRLLRDAQRPLSEVLFANWLWATRAPEELTRFQQDHEAIYEAIAAGDPLGARQAMHRHAQHVASALRAGIA
PAAGELVTTGSDGRHAAA

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI48994955, Length=210, Percent_Identity=30.952380952381, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786303, Length=227, Percent_Identity=35.2422907488987, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790032, Length=230, Percent_Identity=29.5652173913043, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI48994961, Length=224, Percent_Identity=29.4642857142857, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790780, Length=227, Percent_Identity=29.0748898678414, Blast_Score=71, Evalue=9e-14,
Organism=Escherichia coli, GI1789353, Length=226, Percent_Identity=25.6637168141593, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1789621, Length=238, Percent_Identity=28.1512605042017, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI87082208, Length=227, Percent_Identity=27.7533039647577, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 28573; Mature: 28442

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAIPVTAKPITQARLHQRVVEELLRQIVSGALPPGTTLPSEPELARQFGVSRIVIREA
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCHHHHHHHHH
IRILVEKGLITVRHGSGMWVQSIEQWNHLDPMVLLEEVRSSPDASRLSELLELCEILDVG
HHHHHHHCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
AAERAATRRTPEQLSRLRDLVERMRKVVDDPAAYLDLDALFHATIAESAGNRLLRDAQRP
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSEVLFANWLWATRAPEELTRFQQDHEAIYEAIAAGDPLGARQAMHRHAQHVASALRAGI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
APAAGELVTTGSDGRHAAA
CCCCCCEEECCCCCCCCCC
>Mature Secondary Structure 
TRAIPVTAKPITQARLHQRVVEELLRQIVSGALPPGTTLPSEPELARQFGVSRIVIREA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHCHHHHHHHHH
IRILVEKGLITVRHGSGMWVQSIEQWNHLDPMVLLEEVRSSPDASRLSELLELCEILDVG
HHHHHHHCCEEEECCCCHHHHHHHHHCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
AAERAATRRTPEQLSRLRDLVERMRKVVDDPAAYLDLDALFHATIAESAGNRLLRDAQRP
CHHHHHHCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSEVLFANWLWATRAPEELTRFQQDHEAIYEAIAAGDPLGARQAMHRHAQHVASALRAGI
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
APAAGELVTTGSDGRHAAA
CCCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]