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The map label for this gene is braE [H]

Identifier: 221635489

GI number: 221635489

Start: 71481

End: 72461

Strand: Reverse

Name: braE [H]

Synonym: trd_A0083

Alternate gene names: 221635489

Gene position: 72461-71481 (Counterclockwise)

Preceding gene: 221635490

Following gene: 221635488

Centisome position: 7.9

GC content: 63.1

Gene sequence:

>981_bases
ATGAGCCCGCGTGCTGTCGTTGCCGCGCTGATCGTGCTCGCACTTCTCTCCTTCCCTGCCTGGGGCAGTCCTTACTTCGT
GGGACGGATTTTCACACCGGCGTTCATCTTCGGCGTGATCGCGCTGAGCCTCTGTCTTCTGGCCGGCTACGGTGGGATGG
TCTCGCTCGCCCAGATGACGCTTGCTGGTCTGGCCGGCTATGGCTATGGGCACTTGACGGTCATGCAGGGACAGCCCTGG
TGGGTCGGGATTCTCGGCGGCATAGTCCTGGCCACGGTTGTCGCGTTCCTCTTCGGGCTCATCGCGGTGCGCTCCAGCGG
CATCTACTTCCTGATGATCACCCTGGCGCTCGGTATGGTCGTGTATGCGCTGGCCAACCAGAACCGCTCGTTCTTCGGAG
GCCATACCGGCATTCTCGGTATCCGTCCCCCGGAACTCTTCGGTCATGCGCTGACCGAGCGTGTCCCCTTCTATTACCTT
TGCCTGGTCGTCGGCCTCGCGACATACGCTCTGGTTCGCTACTTGGCGCGGACTCCCTTCGGGCTCGCACTCGTGGCGCT
CCGCGACAATCCGCGTCGCCTGGCTGCACTGGGGTTTCATGTTGCCCTCCATCGCGTCGCTGCCTTCACGCTGGCAGGCT
TCCTGGCGGGCGTGGGTGGGTTGCTCCTCGTCTGGTACAACGGTGCCATCTCGCCCACCACGATCGATCTCACGCGACTC
ATCAACATCATGGTCATCGCAGTGATCGGTGGTCTCATGTACGCCGAGGGGGCTTTCCTCGGGGCACTTGTCTTCGTGCT
CGTCACCAACTTCGCCAGTTCCTTCACACAGCGCTTCAACACGGTGATCGGACTCGTCTTCCTGGCGACCGTCCTCTTCT
CGCCCGAGGGGTTGAGTGGGCTGGGCTTGAGAGCGCTCGCGGCGCTCCAGCGCATCCTGCGGGTACGGTTCGCCTCGGCC
GAACGGGCTGAGATGCAGTGA

Upstream 100 bases:

>100_bases
ACAGCCTCGCGCTCTTCCCCACCTATGCAGCCATCGTGAGTTTCTTGCTCATGGTCCTCGTTCTCGCGGTCCGTCCGCAG
GGCCTTTTCGGGAGGCCAGC

Downstream 100 bases:

>100_bases
AGGGAGGTCAGCGCTCGCTTCGACGTCAGTCGGTTCAGTGGTCAGTGTCAGGAGAGGAGGAGCAGCGATGAACGGACAGC
CTGCACGTTTCAGTCGGCGC

Product: ABC transporter ATP-binding protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 325

Protein sequence:

>326_residues
MSPRAVVAALIVLALLSFPAWGSPYFVGRIFTPAFIFGVIALSLCLLAGYGGMVSLAQMTLAGLAGYGYGHLTVMQGQPW
WVGILGGIVLATVVAFLFGLIAVRSSGIYFLMITLALGMVVYALANQNRSFFGGHTGILGIRPPELFGHALTERVPFYYL
CLVVGLATYALVRYLARTPFGLALVALRDNPRRLAALGFHVALHRVAAFTLAGFLAGVGGLLLVWYNGAISPTTIDLTRL
INIMVIAVIGGLMYAEGAFLGALVFVLVTNFASSFTQRFNTVIGLVFLATVLFSPEGLSGLGLRALAALQRILRVRFASA
ERAEMQ

Sequences:

>Translated_326_residues
MSPRAVVAALIVLALLSFPAWGSPYFVGRIFTPAFIFGVIALSLCLLAGYGGMVSLAQMTLAGLAGYGYGHLTVMQGQPW
WVGILGGIVLATVVAFLFGLIAVRSSGIYFLMITLALGMVVYALANQNRSFFGGHTGILGIRPPELFGHALTERVPFYYL
CLVVGLATYALVRYLARTPFGLALVALRDNPRRLAALGFHVALHRVAAFTLAGFLAGVGGLLLVWYNGAISPTTIDLTRL
INIMVIAVIGGLMYAEGAFLGALVFVLVTNFASSFTQRFNTVIGLVFLATVLFSPEGLSGLGLRALAALQRILRVRFASA
ERAEMQ
>Mature_325_residues
SPRAVVAALIVLALLSFPAWGSPYFVGRIFTPAFIFGVIALSLCLLAGYGGMVSLAQMTLAGLAGYGYGHLTVMQGQPWW
VGILGGIVLATVVAFLFGLIAVRSSGIYFLMITLALGMVVYALANQNRSFFGGHTGILGIRPPELFGHALTERVPFYYLC
LVVGLATYALVRYLARTPFGLALVALRDNPRRLAALGFHVALHRVAAFTLAGFLAGVGGLLLVWYNGAISPTTIDLTRLI
NIMVIAVIGGLMYAEGAFLGALVFVLVTNFASSFTQRFNTVIGLVFLATVLFSPEGLSGLGLRALAALQRILRVRFASAE
RAEMQ

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=302, Percent_Identity=24.5033112582781, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34755; Mature: 34624

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPRAVVAALIVLALLSFPAWGSPYFVGRIFTPAFIFGVIALSLCLLAGYGGMVSLAQMT
CCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LAGLAGYGYGHLTVMQGQPWWVGILGGIVLATVVAFLFGLIAVRSSGIYFLMITLALGMV
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VYALANQNRSFFGGHTGILGIRPPELFGHALTERVPFYYLCLVVGLATYALVRYLARTPF
HHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
GLALVALRDNPRRLAALGFHVALHRVAAFTLAGFLAGVGGLLLVWYNGAISPTTIDLTRL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
INIMVIAVIGGLMYAEGAFLGALVFVLVTNFASSFTQRFNTVIGLVFLATVLFSPEGLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LGLRALAALQRILRVRFASAERAEMQ
CHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
SPRAVVAALIVLALLSFPAWGSPYFVGRIFTPAFIFGVIALSLCLLAGYGGMVSLAQMT
CHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
LAGLAGYGYGHLTVMQGQPWWVGILGGIVLATVVAFLFGLIAVRSSGIYFLMITLALGMV
HHHHHCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
VYALANQNRSFFGGHTGILGIRPPELFGHALTERVPFYYLCLVVGLATYALVRYLARTPF
HHHHHCCCCCCCCCCCCEECCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCC
GLALVALRDNPRRLAALGFHVALHRVAAFTLAGFLAGVGGLLLVWYNGAISPTTIDLTRL
CEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHH
INIMVIAVIGGLMYAEGAFLGALVFVLVTNFASSFTQRFNTVIGLVFLATVLFSPEGLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LGLRALAALQRILRVRFASAERAEMQ
CHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]