BacMap

Definition	Thermomicrobium roseum DSM 5159 plasmid unnamed, complete sequence.
Accession	NC_011961
Length	917,738

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The map label for this gene is braG [H]

Identifier: 221635486

GI number: 221635486

Start: 68476

End: 69210

Strand: Reverse

Name: braG [H]

Synonym: trd_A0080

Alternate gene names: 221635486

Gene position: 69210-68476 (Counterclockwise)

Preceding gene: 221635487

Following gene: 221635485

Centisome position: 7.54

GC content: 66.39

Gene sequence:

>735_bases
ATGCCTGACGAGCCGCTTCTCTCGATCCGCGATCTTCATGCGTACTATGGTCAGAGCCATGTCCTGCAGGGTGTTGAACT
CACGGTCGGGCAGGAGCCGCTCGCGCTCCTCGGGCGCAACGGGATGGGGAAGACGACGCTGTGTCTGGCCGTGACAGGGC
TGTTGCCGACAGTCCGCGGCTCGATCCGCTTCGCTGGCCAGGAGCTCGTCGGGAAACCGCCTCATCGCATTGCAGCGCTG
GGCATCGCCTATGTGCCGCAAGGACGCCGCATCTTCCCCTCGCTCACCGTCGAGGAGCATCTCCGGCTCGTACCCCGGCG
ACGACGCGCCGACGGTGGCGAGCCGTGGACGGAGGAGCGAATCTATACCCTGTTCCCTCGCCTCGCTGAACGCCGGCGTC
AGCCGGCCGGTACCCTCTCGGGTGGCGAGCAGCAGATGCTGGCGATCGCCCGTGCTCTGCTGACGAACCCGCGGCTCCTG
ATCATGGACGAACCGTCCGAGGGGCTCGCTCCAGTCATCGTCGAGCAGCTGGTCACCACGCTCCGGCACCTGGCGCAGGA
CGGTCTGGCGATTCTGCTGGTGGAACAGCGACTGGCGGTCGCGACGGCGGTCGCCTCGCGGATCGCCATCATGCTGTCTG
GTCGGATCGTCTGGGAGACGAGTGCAGCGGAACTCTTGGGTGATCCGGAGCGTCAACAGCGCTATCTCGGTGTGAGCGCC
TGGCAGGCTGCTTGA

Upstream 100 bases:

>100_bases
CGGGTGACGGTCCTGCACGAGGGCAGCGTGATCGCCGAGGGGACGCCGGAGGAGATCGCTGCCAATCCGATCGTCCAGCA
GGTCTACCTGGGGGGCGGGT

Downstream 100 bases:

>100_bases
AGGGAGGGCCCGATGCCGACCGTTGTCCTGATCGGAACGCTCGATACGAAGGGAGTGGAGTACGAGTTTCTCCGCCAGCG
CCTGCGCGAACGCGGCTGTG

Product: ABC-type branched-chain amino acid transport systems ATPase component

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MPDEPLLSIRDLHAYYGQSHVLQGVELTVGQEPLALLGRNGMGKTTLCLAVTGLLPTVRGSIRFAGQELVGKPPHRIAAL
GIAYVPQGRRIFPSLTVEEHLRLVPRRRRADGGEPWTEERIYTLFPRLAERRRQPAGTLSGGEQQMLAIARALLTNPRLL
IMDEPSEGLAPVIVEQLVTTLRHLAQDGLAILLVEQRLAVATAVASRIAIMLSGRIVWETSAAELLGDPERQQRYLGVSA
WQAA

Sequences:

>Translated_244_residues
MPDEPLLSIRDLHAYYGQSHVLQGVELTVGQEPLALLGRNGMGKTTLCLAVTGLLPTVRGSIRFAGQELVGKPPHRIAAL
GIAYVPQGRRIFPSLTVEEHLRLVPRRRRADGGEPWTEERIYTLFPRLAERRRQPAGTLSGGEQQMLAIARALLTNPRLL
IMDEPSEGLAPVIVEQLVTTLRHLAQDGLAILLVEQRLAVATAVASRIAIMLSGRIVWETSAAELLGDPERQQRYLGVSA
WQAA
>Mature_243_residues
PDEPLLSIRDLHAYYGQSHVLQGVELTVGQEPLALLGRNGMGKTTLCLAVTGLLPTVRGSIRFAGQELVGKPPHRIAALG
IAYVPQGRRIFPSLTVEEHLRLVPRRRRADGGEPWTEERIYTLFPRLAERRRQPAGTLSGGEQQMLAIARALLTNPRLLI
MDEPSEGLAPVIVEQLVTTLRHLAQDGLAILLVEQRLAVATAVASRIAIMLSGRIVWETSAAELLGDPERQQRYLGVSAW
QAA

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI150417984, Length=187, Percent_Identity=34.7593582887701, Blast_Score=92, Evalue=5e-19,
Organism=Homo sapiens, GI45446740, Length=224, Percent_Identity=29.9107142857143, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI47078218, Length=224, Percent_Identity=29.9107142857143, Blast_Score=89, Evalue=5e-18,
Organism=Homo sapiens, GI6005701, Length=190, Percent_Identity=33.6842105263158, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI31657092, Length=209, Percent_Identity=29.1866028708134, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI27477115, Length=187, Percent_Identity=31.0160427807487, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI153792144, Length=229, Percent_Identity=26.2008733624454, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI27436953, Length=189, Percent_Identity=31.7460317460317, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI105990541, Length=182, Percent_Identity=30.2197802197802, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI21536376, Length=225, Percent_Identity=28.4444444444444, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI116734710, Length=250, Percent_Identity=30, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI27881501, Length=191, Percent_Identity=29.8429319371728, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI30795238, Length=191, Percent_Identity=29.8429319371728, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI46592915, Length=219, Percent_Identity=26.9406392694064, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI46592964, Length=219, Percent_Identity=26.9406392694064, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI46592956, Length=219, Percent_Identity=26.9406392694064, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI27262624, Length=186, Percent_Identity=26.3440860215054, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI27262626, Length=186, Percent_Identity=26.3440860215054, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI46592978, Length=219, Percent_Identity=26.9406392694064, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592898, Length=219, Percent_Identity=26.9406392694064, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI46592971, Length=219, Percent_Identity=26.9406392694064, Blast_Score=65, Evalue=4e-11,
Organism=Homo sapiens, GI11967969, Length=245, Percent_Identity=28.1632653061224, Blast_Score=65, Evalue=7e-11,
Organism=Escherichia coli, GI87082268, Length=239, Percent_Identity=40.5857740585774, Blast_Score=167, Evalue=4e-43,
Organism=Escherichia coli, GI1787792, Length=227, Percent_Identity=31.7180616740088, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1789672, Length=247, Percent_Identity=31.1740890688259, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1789593, Length=234, Percent_Identity=33.7606837606838, Blast_Score=107, Evalue=5e-25,
Organism=Escherichia coli, GI1787029, Length=237, Percent_Identity=29.1139240506329, Blast_Score=103, Evalue=9e-24,
Organism=Escherichia coli, GI1786872, Length=240, Percent_Identity=29.1666666666667, Blast_Score=97, Evalue=9e-22,
Organism=Escherichia coli, GI1789864, Length=259, Percent_Identity=30.1158301158301, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790190, Length=209, Percent_Identity=32.0574162679426, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1787089, Length=246, Percent_Identity=32.1138211382114, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1788225, Length=238, Percent_Identity=32.3529411764706, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790525, Length=234, Percent_Identity=29.4871794871795, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48995001, Length=229, Percent_Identity=30.1310043668122, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87081791, Length=228, Percent_Identity=29.3859649122807, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790162, Length=244, Percent_Identity=27.4590163934426, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788761, Length=245, Percent_Identity=29.7959183673469, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788506, Length=252, Percent_Identity=31.3492063492063, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI48994943, Length=233, Percent_Identity=28.755364806867, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1788644, Length=250, Percent_Identity=28, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787370, Length=239, Percent_Identity=27.6150627615063, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI48994997, Length=252, Percent_Identity=29.7619047619048, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1786563, Length=217, Percent_Identity=30.4147465437788, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI48994883, Length=244, Percent_Identity=26.6393442622951, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1787500, Length=239, Percent_Identity=28.0334728033473, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786253, Length=243, Percent_Identity=28.8065843621399, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1786319, Length=215, Percent_Identity=30.2325581395349, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1788897, Length=200, Percent_Identity=29, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87081834, Length=216, Percent_Identity=31.9444444444444, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1788450, Length=236, Percent_Identity=27.1186440677966, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1788472, Length=219, Percent_Identity=26.4840182648402, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789991, Length=196, Percent_Identity=27.5510204081633, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081709, Length=239, Percent_Identity=27.6150627615063, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1787712, Length=209, Percent_Identity=27.7511961722488, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787759, Length=250, Percent_Identity=26.8, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI87081782, Length=190, Percent_Identity=31.5789473684211, Blast_Score=71, Evalue=8e-14,
Organism=Escherichia coli, GI1788165, Length=217, Percent_Identity=31.3364055299539, Blast_Score=70, Evalue=9e-14,
Organism=Escherichia coli, GI145693107, Length=238, Percent_Identity=29.4117647058824, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786398, Length=206, Percent_Identity=24.7572815533981, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786703, Length=233, Percent_Identity=28.755364806867, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787758, Length=243, Percent_Identity=28.8065843621399, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787105, Length=229, Percent_Identity=28.3842794759825, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1787112, Length=256, Percent_Identity=31.640625, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1788210, Length=199, Percent_Identity=27.6381909547739, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789963, Length=245, Percent_Identity=26.530612244898, Blast_Score=62, Evalue=3e-11,
Organism=Escherichia coli, GI1789032, Length=212, Percent_Identity=26.8867924528302, Blast_Score=61, Evalue=6e-11,
Organism=Escherichia coli, GI1790467, Length=227, Percent_Identity=23.3480176211454, Blast_Score=61, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI115533608, Length=223, Percent_Identity=31.390134529148, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI86563255, Length=174, Percent_Identity=31.0344827586207, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI193209708, Length=235, Percent_Identity=28.0851063829787, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17510237, Length=204, Percent_Identity=29.4117647058824, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17552010, Length=200, Percent_Identity=28.5, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17552232, Length=171, Percent_Identity=33.9181286549708, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17565586, Length=183, Percent_Identity=28.4153005464481, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=237, Percent_Identity=27.8481012658228, Blast_Score=68, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI71983516, Length=182, Percent_Identity=27.4725274725275, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71983510, Length=182, Percent_Identity=27.4725274725275, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71983523, Length=182, Percent_Identity=27.4725274725275, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17538654, Length=200, Percent_Identity=30, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24648314, Length=212, Percent_Identity=30.188679245283, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI24666092, Length=211, Percent_Identity=30.3317535545024, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24643674, Length=211, Percent_Identity=28.436018957346, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI221512771, Length=212, Percent_Identity=27.3584905660377, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28574150, Length=211, Percent_Identity=27.4881516587678, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI116007328, Length=211, Percent_Identity=27.4881516587678, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24650855, Length=228, Percent_Identity=27.6315789473684, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24650853, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI116007184, Length=229, Percent_Identity=27.5109170305677, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI221500365, Length=229, Percent_Identity=27.5109170305677, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI17647959, Length=206, Percent_Identity=34.9514563106796, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI281362751, Length=228, Percent_Identity=27.6315789473684, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24581191, Length=226, Percent_Identity=27.4336283185841, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24581189, Length=226, Percent_Identity=27.4336283185841, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24581193, Length=226, Percent_Identity=27.4336283185841, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24581195, Length=226, Percent_Identity=27.4336283185841, Blast_Score=70, Evalue=9e-13,
Organism=Drosophila melanogaster, GI24582014, Length=237, Percent_Identity=27.8481012658228, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24582012, Length=237, Percent_Identity=27.8481012658228, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574533, Length=202, Percent_Identity=27.2277227722772, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581387, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581383, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24581385, Length=200, Percent_Identity=30.5, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19920532, Length=236, Percent_Identity=27.9661016949153, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24580930, Length=236, Percent_Identity=27.9661016949153, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI85725262, Length=233, Percent_Identity=28.3261802575107, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI23397593, Length=209, Percent_Identity=31.1004784688995, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26764; Mature: 26632

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPDEPLLSIRDLHAYYGQSHVLQGVELTVGQEPLALLGRNGMGKTTLCLAVTGLLPTVRG
CCCCCHHHHHHHHHHHCHHHHHCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHCC
SIRFAGQELVGKPPHRIAALGIAYVPQGRRIFPSLTVEEHLRLVPRRRRADGGEPWTEER
HHEECCHHHCCCCCHHHHHHHHEECCCCCEECCCCCHHHHHHHCCHHHCCCCCCCCHHHH
IYTLFPRLAERRRQPAGTLSGGEQQMLAIARALLTNPRLLIMDEPSEGLAPVIVEQLVTT
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
LRHLAQDGLAILLVEQRLAVATAVASRIAIMLSGRIVWETSAAELLGDPERQQRYLGVSA
HHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHCCHHHHHHHCCCHH
WQAA
HCCC
>Mature Secondary Structure 
PDEPLLSIRDLHAYYGQSHVLQGVELTVGQEPLALLGRNGMGKTTLCLAVTGLLPTVRG
CCCCHHHHHHHHHHHCHHHHHCCEEEEECCCHHHHHCCCCCCHHHHHHHHHHHHHHHCC
SIRFAGQELVGKPPHRIAALGIAYVPQGRRIFPSLTVEEHLRLVPRRRRADGGEPWTEER
HHEECCHHHCCCCCHHHHHHHHEECCCCCEECCCCCHHHHHHHCCHHHCCCCCCCCHHHH
IYTLFPRLAERRRQPAGTLSGGEQQMLAIARALLTNPRLLIMDEPSEGLAPVIVEQLVTT
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
LRHLAQDGLAILLVEQRLAVATAVASRIAIMLSGRIVWETSAAELLGDPERQQRYLGVSA
HHHHHHCCEEEEEHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHCCHHHHHHHCCCHH
WQAA
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]