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The map label for this gene is ppsA [H]

Identifier: 221635481

GI number: 221635481

Start: 63857

End: 64828

Strand: Reverse

Name: ppsA [H]

Synonym: trd_A0075

Alternate gene names: 221635481

Gene position: 64828-63857 (Counterclockwise)

Preceding gene: 221635482

Following gene: 221635480

Centisome position: 7.06

GC content: 67.18

Gene sequence:

>972_bases
ATGAGTCGTGCGGCGACCGTTGCTCTCGTCCGCTGGTTCCACGATCCGGCCTGTACCGACGTCCACCAGGCTGGTGGCAA
GGGGGCAAGCCTCGCCCGCCTGGCCGCTGCTGATCTGCCGGTCCCGCCGGGCTTCGTGGTGACCAGCGCGGCTTTCGCTG
CCTTTCTCGCCGAGAACGGGATCGACGCTCGCATTCGCGCGCTGCTCCAGCGCGTCGACCCCACCGAGCGCCGTGCGCTC
GAGGACGTGGCAGCGGACATCCAAGAGCTCATTCTCACACAGCCCCTTCCACTGGAACTCGATGCTGCCGTCGCTGGCGC
CTATGCCGAACTCGGACATGGCGAGGCGATTCCGGTCGCGGTCCGTTCCAGTGCGGTCGCGGAGGATTCTCAGCAGGCCT
CCTTCGCTGGGCAGCAAGAGACGTTCCTCGATGTCCGCGGTGTGGACGACGTGCTCGCGCGTATCCGCGCCTGCTGGGCC
TCCTTCTTCAGCCCACGAGCGCTCTTCTACCGTGCCCGCAAGGGGTCGCTCGACGATCTTGCCTTCGCGGTCGTCGTGCA
GCAGCTCGTGGTGCCCGAGAAGGCTGGAGTCCTCTTCACTGTCGATCCTGTGCAGCGCCGGCCCGATGTCCTGGTCGTCG
AGGCAGTGTGGGGCTTCGGCGAGGCGCTCGTCTCGGGAGAAGTGACGCCGGACCACTACGAGATCGAGCGTGGGAGTGGA
CGCCTCCTGCGTTGCTTCGTCCCGCCCAAAACGCAGATGCTGGGGCGGGGCGCAACCGGTGGTCTCATCGCTCTCGCGGT
GCCCGAGGAACAACAGCGGTTGCCGGTGCTCAGCGAGGCCGAGCGTGCGGCGCTCGTCGACTTGGCTGTTCGGGTCGAGG
CCTTCTTTGGAGTGCCGCAGGACGTCGAGTGGGCCATCGCTGGCGGCGAGCTTTTTGTCCTGCAGAGTCGCCCGATCACG
ACGCTCGCCTGA

Upstream 100 bases:

>100_bases
CGCGACCGTACGGTTTCAAGGTCGCCGCCTTCCCCATCAAATGGGTCGGCACCACGGCGGCACCCGTCCGCGCGGTCGCC
ATCGTCGAGGAGTAGGAGCG

Downstream 100 bases:

>100_bases
GGAGAGGAGCAGCCGATGTCTGGTGATGGAGAACTCCTCGAGCGGGCACGCAGCTGGGTCGCACCCTACTGGAACGCCCT
GCATCTCTACCGGGCACTCG

Product: phosphoenolpyruvate synthase (Pyruvate, water dikinase)(PEP synthase)

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MSRAATVALVRWFHDPACTDVHQAGGKGASLARLAAADLPVPPGFVVTSAAFAAFLAENGIDARIRALLQRVDPTERRAL
EDVAADIQELILTQPLPLELDAAVAGAYAELGHGEAIPVAVRSSAVAEDSQQASFAGQQETFLDVRGVDDVLARIRACWA
SFFSPRALFYRARKGSLDDLAFAVVVQQLVVPEKAGVLFTVDPVQRRPDVLVVEAVWGFGEALVSGEVTPDHYEIERGSG
RLLRCFVPPKTQMLGRGATGGLIALAVPEEQQRLPVLSEAERAALVDLAVRVEAFFGVPQDVEWAIAGGELFVLQSRPIT
TLA

Sequences:

>Translated_323_residues
MSRAATVALVRWFHDPACTDVHQAGGKGASLARLAAADLPVPPGFVVTSAAFAAFLAENGIDARIRALLQRVDPTERRAL
EDVAADIQELILTQPLPLELDAAVAGAYAELGHGEAIPVAVRSSAVAEDSQQASFAGQQETFLDVRGVDDVLARIRACWA
SFFSPRALFYRARKGSLDDLAFAVVVQQLVVPEKAGVLFTVDPVQRRPDVLVVEAVWGFGEALVSGEVTPDHYEIERGSG
RLLRCFVPPKTQMLGRGATGGLIALAVPEEQQRLPVLSEAERAALVDLAVRVEAFFGVPQDVEWAIAGGELFVLQSRPIT
TLA
>Mature_322_residues
SRAATVALVRWFHDPACTDVHQAGGKGASLARLAAADLPVPPGFVVTSAAFAAFLAENGIDARIRALLQRVDPTERRALE
DVAADIQELILTQPLPLELDAAVAGAYAELGHGEAIPVAVRSSAVAEDSQQASFAGQQETFLDVRGVDDVLARIRACWAS
FFSPRALFYRARKGSLDDLAFAVVVQQLVVPEKAGVLFTVDPVQRRPDVLVVEAVWGFGEALVSGEVTPDHYEIERGSGR
LLRCFVPPKTQMLGRGATGGLIALAVPEEQQRLPVLSEAERAALVDLAVRVEAFFGVPQDVEWAIAGGELFVLQSRPITT
LA

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=345, Percent_Identity=35.6521739130435, Blast_Score=192, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI17564524, Length=318, Percent_Identity=32.3899371069182, Blast_Score=134, Evalue=6e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 34539; Mature: 34408

Theoretical pI: Translated: 4.63; Mature: 4.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRAATVALVRWFHDPACTDVHQAGGKGASLARLAAADLPVPPGFVVTSAAFAAFLAENG
CCCHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
IDARIRALLQRVDPTERRALEDVAADIQELILTQPLPLELDAAVAGAYAELGHGEAIPVA
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCEEEEE
VRSSAVAEDSQQASFAGQQETFLDVRGVDDVLARIRACWASFFSPRALFYRARKGSLDDL
ECCHHHHCCCHHHHCCCCCHHEEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHH
AFAVVVQQLVVPEKAGVLFTVDPVQRRPDVLVVEAVWGFGEALVSGEVTPDHYEIERGSG
HHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEHHCCHHHHHCCCCCCCCEEEECCCC
RLLRCFVPPKTQMLGRGATGGLIALAVPEEQQRLPVLSEAERAALVDLAVRVEAFFGVPQ
CEEEEECCCCHHHHCCCCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DVEWAIAGGELFVLQSRPITTLA
CCEEEEECCEEEEEECCCCCCCC
>Mature Secondary Structure 
SRAATVALVRWFHDPACTDVHQAGGKGASLARLAAADLPVPPGFVVTSAAFAAFLAENG
CCHHHHHHHHHHCCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
IDARIRALLQRVDPTERRALEDVAADIQELILTQPLPLELDAAVAGAYAELGHGEAIPVA
CCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHCCCCCEEEEE
VRSSAVAEDSQQASFAGQQETFLDVRGVDDVLARIRACWASFFSPRALFYRARKGSLDDL
ECCHHHHCCCHHHHCCCCCHHEEEECCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCHHHH
AFAVVVQQLVVPEKAGVLFTVDPVQRRPDVLVVEAVWGFGEALVSGEVTPDHYEIERGSG
HHHHHHHHHHCCCCCCEEEEECCCCCCCCEEEEEEHHCCHHHHHCCCCCCCCEEEECCCC
RLLRCFVPPKTQMLGRGATGGLIALAVPEEQQRLPVLSEAERAALVDLAVRVEAFFGVPQ
CEEEEECCCCHHHHCCCCCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCC
DVEWAIAGGELFVLQSRPITTLA
CCEEEEECCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]