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The map label for this gene is yvkC [H]

Identifier: 221635476

GI number: 221635476

Start: 59297

End: 60991

Strand: Reverse

Name: yvkC [H]

Synonym: trd_A0070

Alternate gene names: 221635476

Gene position: 60991-59297 (Counterclockwise)

Preceding gene: 221635477

Following gene: 221635475

Centisome position: 6.65

GC content: 64.25

Gene sequence:

>1695_bases
ATGGCGGCGCAGCGACCTGAGGTGCAGCTGCCGGTGACATTCTTCGGCGACGAGTCGTTCCCGGTCGAATGGGAGAACGA
GGAGGAAAAGCAGCTTTTCTGGTGGTGGGACGATCTTCACTGCCCGAATCCGCTCTCGCCGATGTTCTTCGAACTCGGCG
GCTGGTGGGCGACCTGTGCCTATCTCTATCGGCGCTTTGGAGCCCCCTTCGGCCGCGACTGGAAGGCCAAGCTGATCAAC
GGGTATCTGTTCACAGCTGTTGTCCCGCGTGATCCCAAGGAGGCAGCTGAACTCGCCCCGTACTATAGCATGGTCATGCC
GGTCTATGCGGAAAAAGGTCTCGAGTGGTGGCAGAAGCGGCTGCGACCGGAGATCGAGCGCAACTTCGAGTATCTCGATA
CCTACCCCTATGACACGGCGACGCTGCCCGAGCTGATGGTGCTCCTGGAAGACGCGATCGATATCCAGGAGCGCCACTGG
CGTATTCACTGGATTCTCAATCTCTCCCAGTTCCAGGCCTCGCTCGACTTCCAGGCTGCGGTGCGCGAGGTCATCGGCGA
CGTCGATCCGACGCTGCTCGAACGCATCATGGTCTCCGATAGCGACCGCAACTGGGATGCCGTGCACGGGCTGTGGGAAC
TCAAGGAGCGCGTCAAGCGCAACGTGGTGCTCCTGGAGGCCTTCAGCAAGGAAACGCCGGCCGAGATTCTCGCCGAACTG
GAGAAGACGGCGGAGGGTCGCGAGTTTCTACGCTGGCTCGACGAATACAAGCGCGAGTTCGGCCACAAGGCTCTCTACAG
CCACGAGTACGTCTATCCGACCTGGTACGAAAACCCGGCACCGATCATCGCGGCCATCCAGGGGTATCTCCAGACCGACT
ACTCCTACCCGGAGGCGATCCGACGCCTGCGCCAGGATCGCGATGCGGCCATCCAGGAACTCCTGAGCAAAATCCCACCA
GACGACGCACGGGGGCGCGAGCGTGTCGAGGCAGCGCTCCATCGCGTGCTCCAGATGATGCCGCTCACACCCGATCACCA
CTTCTATATCGACCAGGGGACGTTTGCGCGCCTGCGCTACGTGCTCCTGGCGATCGGCCGGCGCCTGGTGGAGGCCGAGC
TTCTCGATCAACCGGATGACGTGCTCTTCCTGCGCTATCACGAGCTGCGTGTCCTGGCGGCGGAGCCCTCCAACCTGCCC
AATGCCAAGGAGTTGGTCCGCGAGCGACGAGCTGCCCGCGAGCGTGCCATGGCCGTGCGTCCGCGCGATTGGGTCGGCAC
TGCCAGCGAATGGGCGCTCTTCCAGGAGCCGTACAAGACGCTCTGGGGGTACCCGCAGCGGTTCTTCGACTCGCTCCAGC
CGCGCCAGGTGAGCCAGCGGGTCGAGGGGATCCCGGCCTCGGCTGGGGTGGTGGAGGGAACCGCCTTCGTCGCCCGCACG
CTGGAGGACGTCGATGCGCTCAAAGGGGGCGAGATCCTGGTCTGCCGCATGACCAACCCGGCGTGGGTCATCGCCTTCAC
CAAGATCGCTGGCCTGGTGACCGATTCCGGTGGGCAGCTCTCGCACCCTGCCGTCGTCTCGCGGGAGTTCGGCATCCCGG
CGGTCGTCGGGACGGGGGTTGCCACGCAGGTCATCCGGACCGGGCAGCGCATCCGCGTCAACGGCTCGGCCGGTGTGGTC
GAGATTCTCGAGTAG

Upstream 100 bases:

>100_bases
CAACACCGTCGTGCCGATGCTGGAGAGTCGCGTGCCCGAGTTCTACGACCTGATCGAGCCGGAGTGATGGTGGACCGCAG
CCACAGGAAGGAGGGAAATC

Downstream 100 bases:

>100_bases
GCGCACGTCGGGCGCAGAGCGCCACCTGAAGAGCGCTGCGACGAGGAGGTGCGGCGAGGCGTCTGGAGCGTGGGTGCGCG
GGTCTCTTGCGGTGCGGATG

Product: PEP-utilising enzyme, mobile region

Products: AMP; H+; Phosphoenolpyruvate; Phosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MAAQRPEVQLPVTFFGDESFPVEWENEEEKQLFWWWDDLHCPNPLSPMFFELGGWWATCAYLYRRFGAPFGRDWKAKLIN
GYLFTAVVPRDPKEAAELAPYYSMVMPVYAEKGLEWWQKRLRPEIERNFEYLDTYPYDTATLPELMVLLEDAIDIQERHW
RIHWILNLSQFQASLDFQAAVREVIGDVDPTLLERIMVSDSDRNWDAVHGLWELKERVKRNVVLLEAFSKETPAEILAEL
EKTAEGREFLRWLDEYKREFGHKALYSHEYVYPTWYENPAPIIAAIQGYLQTDYSYPEAIRRLRQDRDAAIQELLSKIPP
DDARGRERVEAALHRVLQMMPLTPDHHFYIDQGTFARLRYVLLAIGRRLVEAELLDQPDDVLFLRYHELRVLAAEPSNLP
NAKELVRERRAARERAMAVRPRDWVGTASEWALFQEPYKTLWGYPQRFFDSLQPRQVSQRVEGIPASAGVVEGTAFVART
LEDVDALKGGEILVCRMTNPAWVIAFTKIAGLVTDSGGQLSHPAVVSREFGIPAVVGTGVATQVIRTGQRIRVNGSAGVV
EILE

Sequences:

>Translated_564_residues
MAAQRPEVQLPVTFFGDESFPVEWENEEEKQLFWWWDDLHCPNPLSPMFFELGGWWATCAYLYRRFGAPFGRDWKAKLIN
GYLFTAVVPRDPKEAAELAPYYSMVMPVYAEKGLEWWQKRLRPEIERNFEYLDTYPYDTATLPELMVLLEDAIDIQERHW
RIHWILNLSQFQASLDFQAAVREVIGDVDPTLLERIMVSDSDRNWDAVHGLWELKERVKRNVVLLEAFSKETPAEILAEL
EKTAEGREFLRWLDEYKREFGHKALYSHEYVYPTWYENPAPIIAAIQGYLQTDYSYPEAIRRLRQDRDAAIQELLSKIPP
DDARGRERVEAALHRVLQMMPLTPDHHFYIDQGTFARLRYVLLAIGRRLVEAELLDQPDDVLFLRYHELRVLAAEPSNLP
NAKELVRERRAARERAMAVRPRDWVGTASEWALFQEPYKTLWGYPQRFFDSLQPRQVSQRVEGIPASAGVVEGTAFVART
LEDVDALKGGEILVCRMTNPAWVIAFTKIAGLVTDSGGQLSHPAVVSREFGIPAVVGTGVATQVIRTGQRIRVNGSAGVV
EILE
>Mature_563_residues
AAQRPEVQLPVTFFGDESFPVEWENEEEKQLFWWWDDLHCPNPLSPMFFELGGWWATCAYLYRRFGAPFGRDWKAKLING
YLFTAVVPRDPKEAAELAPYYSMVMPVYAEKGLEWWQKRLRPEIERNFEYLDTYPYDTATLPELMVLLEDAIDIQERHWR
IHWILNLSQFQASLDFQAAVREVIGDVDPTLLERIMVSDSDRNWDAVHGLWELKERVKRNVVLLEAFSKETPAEILAELE
KTAEGREFLRWLDEYKREFGHKALYSHEYVYPTWYENPAPIIAAIQGYLQTDYSYPEAIRRLRQDRDAAIQELLSKIPPD
DARGRERVEAALHRVLQMMPLTPDHHFYIDQGTFARLRYVLLAIGRRLVEAELLDQPDDVLFLRYHELRVLAAEPSNLPN
AKELVRERRAARERAMAVRPRDWVGTASEWALFQEPYKTLWGYPQRFFDSLQPRQVSQRVEGIPASAGVVEGTAFVARTL
EDVDALKGGEILVCRMTNPAWVIAFTKIAGLVTDSGGQLSHPAVVSREFGIPAVVGTGVATQVIRTGQRIRVNGSAGVVE
ILE

Specific function: ESSENTIAL STEP IN GLUCONEOGENESIS WHEN PYRUVATE AND LACTATE ARE USED AS A CARBON SOURCE. [C]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=86, Percent_Identity=36.046511627907, Blast_Score=69, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17564524, Length=347, Percent_Identity=26.8011527377522, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR002192 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF01326 PPDK_N [H]

EC number: 2.7.9.2 [C]

Molecular weight: Translated: 65110; Mature: 64979

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAQRPEVQLPVTFFGDESFPVEWENEEEKQLFWWWDDLHCPNPLSPMFFELGGWWATCA
CCCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCHHHHHH
YLYRRFGAPFGRDWKAKLINGYLFTAVVPRDPKEAAELAPYYSMVMPVYAEKGLEWWQKR
HHHHHHCCCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRPEIERNFEYLDTYPYDTATLPELMVLLEDAIDIQERHWRIHWILNLSQFQASLDFQAA
CCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHHCCHHHH
VREVIGDVDPTLLERIMVSDSDRNWDAVHGLWELKERVKRNVVLLEAFSKETPAEILAEL
HHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEEHHCCCCHHHHHHHH
EKTAEGREFLRWLDEYKREFGHKALYSHEYVYPTWYENPAPIIAAIQGYLQTDYSYPEAI
HHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
RRLRQDRDAAIQELLSKIPPDDARGRERVEAALHRVLQMMPLTPDHHFYIDQGTFARLRY
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHH
VLLAIGRRLVEAELLDQPDDVLFLRYHELRVLAAEPSNLPNAKELVRERRAARERAMAVR
HHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
PRDWVGTASEWALFQEPYKTLWGYPQRFFDSLQPRQVSQRVEGIPASAGVVEGTAFVART
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
LEDVDALKGGEILVCRMTNPAWVIAFTKIAGLVTDSGGQLSHPAVVSREFGIPAVVGTGV
HHHHHHCCCCCEEEEEECCCEEEEEHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHCHH
ATQVIRTGQRIRVNGSAGVVEILE
HHHHHHCCCEEEECCCCCEEEECC
>Mature Secondary Structure 
AAQRPEVQLPVTFFGDESFPVEWENEEEKQLFWWWDDLHCPNPLSPMFFELGGWWATCA
CCCCCCEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHCCHHHHHH
YLYRRFGAPFGRDWKAKLINGYLFTAVVPRDPKEAAELAPYYSMVMPVYAEKGLEWWQKR
HHHHHHCCCCCCCHHHHHHCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LRPEIERNFEYLDTYPYDTATLPELMVLLEDAIDIQERHWRIHWILNLSQFQASLDFQAA
CCHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCEEEEEEEEHHHHHHHCCHHHH
VREVIGDVDPTLLERIMVSDSDRNWDAVHGLWELKERVKRNVVLLEAFSKETPAEILAEL
HHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCEEEEEHHCCCCHHHHHHHH
EKTAEGREFLRWLDEYKREFGHKALYSHEYVYPTWYENPAPIIAAIQGYLQTDYSYPEAI
HHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHH
RRLRQDRDAAIQELLSKIPPDDARGRERVEAALHRVLQMMPLTPDHHFYIDQGTFARLRY
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHHH
VLLAIGRRLVEAELLDQPDDVLFLRYHELRVLAAEPSNLPNAKELVRERRAARERAMAVR
HHHHHHHHHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCC
PRDWVGTASEWALFQEPYKTLWGYPQRFFDSLQPRQVSQRVEGIPASAGVVEGTAFVART
CCCCCCCCHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHH
LEDVDALKGGEILVCRMTNPAWVIAFTKIAGLVTDSGGQLSHPAVVSREFGIPAVVGTGV
HHHHHHCCCCCEEEEEECCCEEEEEHHHHHHHHCCCCCCCCCCCHHHHCCCCCHHHHCHH
ATQVIRTGQRIRVNGSAGVVEILE
HHHHHHCCCEEEECCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.083 {pyruvate}} 10.5 {phosphate}} 0.028 {ATP}} [C]

Substrates: ATP; H2O; Pyruvate [C]

Specific reaction: ATP + H2O + Pyruvate --> AMP + (2) H+ + Phosphoenolpyruvate + Phosphate [C]

General reaction: Phospho group transfer [C]

Inhibitor: 2-Oxoglutarate; 3-Phosphoglyceraldehyde; 5'-Adenylyl methylen ediphosphonate; ADP; ADP glucose; AMP; ATP; Ca2+; F-; Iodoacetate; Malate; Mg2+; Mn2+; Oxalacetate; PCMB; Phosphoenolpyruvate; Sulfhydryl reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]