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The map label for this gene is kefB [C]

Identifier: 221633949

GI number: 221633949

Start: 1995654

End: 1996862

Strand: Reverse

Name: kefB [C]

Synonym: trd_1977

Alternate gene names: 221633949

Gene position: 1996862-1995654 (Counterclockwise)

Preceding gene: 221633952

Following gene: 221633948

Centisome position: 99.69

GC content: 62.12

Gene sequence:

>1209_bases
ATGGCAAACATCTGGGCCACTGCCGCACTCTGGATCGGACTCGCATTTTTGGCGAGCATCATCTCCATTCGCCTCGGACT
GTCCGTCGCACTCGTCGAGATCCTGGTCGGTATCGTCGCCGGGAATACGCTCGGCCTCCAGACCGATACCTGGATCAATG
CGCTCGCCAGTTTTGGGGCGGTCGTCCTCACCTTCCTCGCCGGGGCGGAGATCGAGCCGGAAGCCCTGCGCCGGCACCTC
AAGGCGAGTCTCGCGATCGGCTTCGCTGGATTCCTCGCGCCGTTTCTCGGTGCCACCGCCTTCACACGCTTCGTCGCGGG
TTGGAGCTGGCCACAAGCCTGGATCGGTGGCCTCGCTCTCTCCACCACTTCGGTCGCCGTCGTCTATGCGGTGATGGTGG
AAACCGGACTGAACGAGACCGACATCGGCAAGTTGATCCTGGCCGCCTGCTTCGTCAACGACTTGGGCACGGTTCTCGCA
TTGGGGCTTCTCTTTGCGAACTATGACCTGCACCTCGTGGTTTTCGTCGTCGTCACGGCAGTCACGCTCTTCCTGCTCGG
GCGAGTGACACGATTCGTGATCGCAGCGTGGGGAAACCGCGTCAGCGAGCCAGAAGTCAAATTCCTGCTCCTGGTACTCT
TCTTCCTCGGTTGGCTGGCGACGATCGCCAAGAGCGAAGCTGTTCTGCCAGCGTACTTGGTCGGCCTGGCCATCGCTGAG
ATTTTCACGCATCATCGCCCACTCATGCTGCGCCTGCGTACCCTGGCCTTCGCTCTGCTCACGCCATGGTACTTCCTCAA
GGCAGGCTTGTACGTTTCCCTGCCAGCGCTCGTCAGCAGTATCGGGTTGATCGTCGCGCTGCTCTTGGTCAAGATGGTCA
CGAAGCTTCTCGGAGTCGGACCCTTGACGCTCGTCTTCGGGCTCGAGCGGCGCACGAGCGCCTATACCACCCTGCTGATG
TCGACAGGCCTGACTTTTGGTACTATTTCCGCACTCTTCGGCCTCAACAACGGCATCATCGACCGCACCCAGTACACTGC
ACTGGTGACCGCGGTGATCGCCTCCGCGTTGGTACCCACGTGGATCGCACAGACCTGGTTCCTGCCACGCACCGAGGAGA
CGCCGCGCGCCCCGGCCGACACCGCGGCACCTCTGCCTGGAGTGGAGACGGAACTCGTCGCCGAGCGAGCGACACGAGAG
GACGGTTGA

Upstream 100 bases:

>100_bases
GAAGGCTGATGGCTCCTGCTCGCAGGACGTGCGGGCAGGAGTTTTTCTTCTGCCCCCGTCCTCGAAAACTGCGAACGGAA
CGAGCGGGAAGGGGGCATCG

Downstream 100 bases:

>100_bases
GCGATGTACCGCAAAATTCTCGTCGGATTCGATGGTTCACCAGGGGCACAGGCGGCCCTGCGCCACGCCATCGAGCTGGC
GCGCCTGTTCGGGGCTGAAT

Product: Kef-type K+ transport systems, membrane components

Products: Proton [Cytoplasm]; K (I) [Periplasm] [C]

Alternate protein names: MjNapA [H]

Number of amino acids: Translated: 402; Mature: 401

Protein sequence:

>402_residues
MANIWATAALWIGLAFLASIISIRLGLSVALVEILVGIVAGNTLGLQTDTWINALASFGAVVLTFLAGAEIEPEALRRHL
KASLAIGFAGFLAPFLGATAFTRFVAGWSWPQAWIGGLALSTTSVAVVYAVMVETGLNETDIGKLILAACFVNDLGTVLA
LGLLFANYDLHLVVFVVVTAVTLFLLGRVTRFVIAAWGNRVSEPEVKFLLLVLFFLGWLATIAKSEAVLPAYLVGLAIAE
IFTHHRPLMLRLRTLAFALLTPWYFLKAGLYVSLPALVSSIGLIVALLLVKMVTKLLGVGPLTLVFGLERRTSAYTTLLM
STGLTFGTISALFGLNNGIIDRTQYTALVTAVIASALVPTWIAQTWFLPRTEETPRAPADTAAPLPGVETELVAERATRE
DG

Sequences:

>Translated_402_residues
MANIWATAALWIGLAFLASIISIRLGLSVALVEILVGIVAGNTLGLQTDTWINALASFGAVVLTFLAGAEIEPEALRRHL
KASLAIGFAGFLAPFLGATAFTRFVAGWSWPQAWIGGLALSTTSVAVVYAVMVETGLNETDIGKLILAACFVNDLGTVLA
LGLLFANYDLHLVVFVVVTAVTLFLLGRVTRFVIAAWGNRVSEPEVKFLLLVLFFLGWLATIAKSEAVLPAYLVGLAIAE
IFTHHRPLMLRLRTLAFALLTPWYFLKAGLYVSLPALVSSIGLIVALLLVKMVTKLLGVGPLTLVFGLERRTSAYTTLLM
STGLTFGTISALFGLNNGIIDRTQYTALVTAVIASALVPTWIAQTWFLPRTEETPRAPADTAAPLPGVETELVAERATRE
DG
>Mature_401_residues
ANIWATAALWIGLAFLASIISIRLGLSVALVEILVGIVAGNTLGLQTDTWINALASFGAVVLTFLAGAEIEPEALRRHLK
ASLAIGFAGFLAPFLGATAFTRFVAGWSWPQAWIGGLALSTTSVAVVYAVMVETGLNETDIGKLILAACFVNDLGTVLAL
GLLFANYDLHLVVFVVVTAVTLFLLGRVTRFVIAAWGNRVSEPEVKFLLLVLFFLGWLATIAKSEAVLPAYLVGLAIAEI
FTHHRPLMLRLRTLAFALLTPWYFLKAGLYVSLPALVSSIGLIVALLLVKMVTKLLGVGPLTLVFGLERRTSAYTTLLMS
TGLTFGTISALFGLNNGIIDRTQYTALVTAVIASALVPTWIAQTWFLPRTEETPRAPADTAAPLPGVETELVAERATRED
G

Specific function: This is probably a Na(+)/H(+) antiporter [H]

COG id: COG0475

COG function: function code P; Kef-type K+ transport systems, membrane components

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006153 [H]

Pfam domain/function: PF00999 Na_H_Exchanger [H]

EC number: NA

Molecular weight: Translated: 42981; Mature: 42850

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANIWATAALWIGLAFLASIISIRLGLSVALVEILVGIVAGNTLGLQTDTWINALASFGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VVLTFLAGAEIEPEALRRHLKASLAIGFAGFLAPFLGATAFTRFVAGWSWPQAWIGGLAL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHH
STTSVAVVYAVMVETGLNETDIGKLILAACFVNDLGTVLALGLLFANYDLHLVVFVVVTA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VTLFLLGRVTRFVIAAWGNRVSEPEVKFLLLVLFFLGWLATIAKSEAVLPAYLVGLAIAE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IFTHHRPLMLRLRTLAFALLTPWYFLKAGLYVSLPALVSSIGLIVALLLVKMVTKLLGVG
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLTLVFGLERRTSAYTTLLMSTGLTFGTISALFGLNNGIIDRTQYTALVTAVIASALVPT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
WIAQTWFLPRTEETPRAPADTAAPLPGVETELVAERATREDG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
ANIWATAALWIGLAFLASIISIRLGLSVALVEILVGIVAGNTLGLQTDTWINALASFGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
VVLTFLAGAEIEPEALRRHLKASLAIGFAGFLAPFLGATAFTRFVAGWSWPQAWIGGLAL
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHH
STTSVAVVYAVMVETGLNETDIGKLILAACFVNDLGTVLALGLLFANYDLHLVVFVVVTA
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
VTLFLLGRVTRFVIAAWGNRVSEPEVKFLLLVLFFLGWLATIAKSEAVLPAYLVGLAIAE
HHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
IFTHHRPLMLRLRTLAFALLTPWYFLKAGLYVSLPALVSSIGLIVALLLVKMVTKLLGVG
HHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLTLVFGLERRTSAYTTLLMSTGLTFGTISALFGLNNGIIDRTQYTALVTAVIASALVPT
HHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
WIAQTWFLPRTEETPRAPADTAAPLPGVETELVAERATREDG
HHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; K (I) [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + K (I) [Cytoplasm] = Proton [Cytoplasm] + K (I) [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]