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The map label for this gene is gutB [H]

Identifier: 221633915

GI number: 221633915

Start: 1960099

End: 1961340

Strand: Reverse

Name: gutB [H]

Synonym: trd_1942

Alternate gene names: 221633915

Gene position: 1961340-1960099 (Counterclockwise)

Preceding gene: 221633916

Following gene: 221633912

Centisome position: 97.92

GC content: 66.1

Gene sequence:

>1242_bases
ATGCTCGCGCTCCAGATCGTGGAGAGTGTACCGCGCTATCTCTTGGCCAAGGCGGTCGGTCGCTGGCAACCTGCGGCGTA
CTGGAACGGCCTCGGGCTTCTCCAGTTACGCGACGTCCCCGAGCCCCGCCTGCCGACACCAGAATGGGTCAGGATCACCA
CGCGCTACAGTGGGATCTGTGGAAGCGACGTCGGACTGATCACCCTGCACGCCAGCCCGACCACCTCGCCGCTGGTCTCT
TTCCCTTTCACGCTCGGCCATGAGGTAACCGGACAGATCAGCGAGCTCGGCTCAGCAGTGGATGGCTTTCGGGTCGGGCA
ACGCGTCACGGTCAACCCGCTGCTCGCGTGTGCCGCACGCGGTTTTCGGGAACCATGTAGCGAGTGCGCGCGTGGTCTCC
CGAACCGCTGTCTCCGCTTCCGCGAAGGCACGATCGCGCCCGGTATCATGATCGGCTTCTGCCGCGACACGGGTGGAGGC
TGGAGCGCCAGTTTCGTCGCTCACCGAGCACAGCTGATCCCGATCCCTGACCAGCTGACAGACGAGGAAGCCGTGTTGGC
CGAGCCGTTCGGTGTCGCGCTCCATGCAGTGCTCCAAAACCGACCTCCCGACACAGCGACCGTTCTCGTCCTCGGTGCTG
GCATCATCGGACTCCTGACGGTCGCCGCACTGCGGGCGATCGGATCGCAAGCCCGCATCCTGGTGACGGCAAAGTATCCG
TTCCAAGCCCGAGCTGCCGAGCGACTCGGAGCCGATCTCGTTCTCCTGGGGCGGACAGGCCCGGCGCTCTATCGCGAGAT
CGCGCGCCTCACGGGTGCGCGTGTCCTGCGTGCACGCTTCGGTCGCTGGTTGGTCCAGGGTGGGGTCGACCAGGTATTCG
ACTGTGTGGGTTCCAGTCGGTCCGTCCACGACGCGCTCACGCTCACGCGGGCTGGCGGGCGCGTGGTCCTCGTCGGTCTG
GCCAGCGACCCACGCAACGTGGACTGGACGCCGATCTGGCTGCGCGAGGTCGAGATCCGTGGGTCGATGGCCTATGCGGA
GGAAGAACTGGACGGCATCCGTGCCTCGAGCATCGCCCATGGAGTGCGGCTGATGGCCGAGCGTCGCGTCGAGTTGGGCT
GGCTCGTCACCCATCGCTTCGCACTCGCCGAGTACCAGGAAGCGCTGGCGACGGTGACTGGCAAAGGGGCGAACGGCGTC
ATCAAGGCCGTCTTCGCCTTTTCCGGCGGCAACCGAGTCTGA

Upstream 100 bases:

>100_bases
GTTTGGGTCAACGGCGTCTGCGTGCTCCGCGACGGTATCCACACCGGAGCCATGCCAGGCCGCATCGTCTCCGGCCCGGC
CCGTGCGTAAGGGAGGATCG

Downstream 100 bases:

>100_bases
GGGGATCACCGCTCGAGCGCAACCGAACCGAGACGACATCCCCAGTAGGTTACGCTCGCTGCGATCAGGCCATCGGGACT
TGCGGGATCAGCTTCCCATC

Product: putative alcohol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MLALQIVESVPRYLLAKAVGRWQPAAYWNGLGLLQLRDVPEPRLPTPEWVRITTRYSGICGSDVGLITLHASPTTSPLVS
FPFTLGHEVTGQISELGSAVDGFRVGQRVTVNPLLACAARGFREPCSECARGLPNRCLRFREGTIAPGIMIGFCRDTGGG
WSASFVAHRAQLIPIPDQLTDEEAVLAEPFGVALHAVLQNRPPDTATVLVLGAGIIGLLTVAALRAIGSQARILVTAKYP
FQARAAERLGADLVLLGRTGPALYREIARLTGARVLRARFGRWLVQGGVDQVFDCVGSSRSVHDALTLTRAGGRVVLVGL
ASDPRNVDWTPIWLREVEIRGSMAYAEEELDGIRASSIAHGVRLMAERRVELGWLVTHRFALAEYQEALATVTGKGANGV
IKAVFAFSGGNRV

Sequences:

>Translated_413_residues
MLALQIVESVPRYLLAKAVGRWQPAAYWNGLGLLQLRDVPEPRLPTPEWVRITTRYSGICGSDVGLITLHASPTTSPLVS
FPFTLGHEVTGQISELGSAVDGFRVGQRVTVNPLLACAARGFREPCSECARGLPNRCLRFREGTIAPGIMIGFCRDTGGG
WSASFVAHRAQLIPIPDQLTDEEAVLAEPFGVALHAVLQNRPPDTATVLVLGAGIIGLLTVAALRAIGSQARILVTAKYP
FQARAAERLGADLVLLGRTGPALYREIARLTGARVLRARFGRWLVQGGVDQVFDCVGSSRSVHDALTLTRAGGRVVLVGL
ASDPRNVDWTPIWLREVEIRGSMAYAEEELDGIRASSIAHGVRLMAERRVELGWLVTHRFALAEYQEALATVTGKGANGV
IKAVFAFSGGNRV
>Mature_413_residues
MLALQIVESVPRYLLAKAVGRWQPAAYWNGLGLLQLRDVPEPRLPTPEWVRITTRYSGICGSDVGLITLHASPTTSPLVS
FPFTLGHEVTGQISELGSAVDGFRVGQRVTVNPLLACAARGFREPCSECARGLPNRCLRFREGTIAPGIMIGFCRDTGGG
WSASFVAHRAQLIPIPDQLTDEEAVLAEPFGVALHAVLQNRPPDTATVLVLGAGIIGLLTVAALRAIGSQARILVTAKYP
FQARAAERLGADLVLLGRTGPALYREIARLTGARVLRARFGRWLVQGGVDQVFDCVGSSRSVHDALTLTRAGGRVVLVGL
ASDPRNVDWTPIWLREVEIRGSMAYAEEELDGIRASSIAHGVRLMAERRVELGWLVTHRFALAEYQEALATVTGKGANGV
IKAVFAFSGGNRV

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=365, Percent_Identity=30.4109589041096, Blast_Score=127, Evalue=2e-29,
Organism=Escherichia coli, GI1787863, Length=354, Percent_Identity=26.8361581920904, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI226510992, Length=352, Percent_Identity=29.8295454545455, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1788073, Length=368, Percent_Identity=27.1739130434783, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1788075, Length=389, Percent_Identity=25.706940874036, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI87082125, Length=388, Percent_Identity=29.8969072164948, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1790045, Length=359, Percent_Identity=23.9554317548747, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI1788407, Length=366, Percent_Identity=25.4098360655738, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1790718, Length=294, Percent_Identity=25.5102040816327, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17562876, Length=369, Percent_Identity=30.0813008130081, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI17562878, Length=386, Percent_Identity=30.0518134715026, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6319258, Length=376, Percent_Identity=27.1276595744681, Blast_Score=113, Evalue=6e-26,
Organism=Saccharomyces cerevisiae, GI6323099, Length=372, Percent_Identity=24.4623655913978, Blast_Score=109, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6319955, Length=388, Percent_Identity=23.9690721649485, Blast_Score=106, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6322619, Length=388, Percent_Identity=23.9690721649485, Blast_Score=106, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6319257, Length=348, Percent_Identity=25.5747126436782, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI17137530, Length=382, Percent_Identity=30.6282722513089, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17737897, Length=381, Percent_Identity=28.6089238845144, Blast_Score=108, Evalue=9e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 44453; Mature: 44453

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALQIVESVPRYLLAKAVGRWQPAAYWNGLGLLQLRDVPEPRLPTPEWVRITTRYSGIC
CHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCC
GSDVGLITLHASPTTSPLVSFPFTLGHEVTGQISELGSAVDGFRVGQRVTVNPLLACAAR
CCCCCEEEEECCCCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
GFREPCSECARGLPNRCLRFREGTIAPGIMIGFCRDTGGGWSASFVAHRAQLIPIPDQLT
HHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHCCEEECCCCCCC
DEEAVLAEPFGVALHAVLQNRPPDTATVLVLGAGIIGLLTVAALRAIGSQARILVTAKYP
CCCHHHHCHHHHHHHHHHHCCCCCHHEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
FQARAAERLGADLVLLGRTGPALYREIARLTGARVLRARFGRWLVQGGVDQVFDCVGSSR
CHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
SVHDALTLTRAGGRVVLVGLASDPRNVDWTPIWLREVEIRGSMAYAEEELDGIRASSIAH
CHHHHHEEEECCCEEEEEEECCCCCCCCCCEEEEEEEEECCCHHHHHHHHCCHHHHHHHH
GVRLMAERRVELGWLVTHRFALAEYQEALATVTGKGANGVIKAVFAFSGGNRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MLALQIVESVPRYLLAKAVGRWQPAAYWNGLGLLQLRDVPEPRLPTPEWVRITTRYSGIC
CHHHHHHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCCCCCCCCCEEEEEEEECCCC
GSDVGLITLHASPTTSPLVSFPFTLGHEVTGQISELGSAVDGFRVGQRVTVNPLLACAAR
CCCCCEEEEECCCCCCCCEECCHHCCCHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHH
GFREPCSECARGLPNRCLRFREGTIAPGIMIGFCRDTGGGWSASFVAHRAQLIPIPDQLT
HHHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEEECCCCCCHHHHHHHCCEEECCCCCCC
DEEAVLAEPFGVALHAVLQNRPPDTATVLVLGAGIIGLLTVAALRAIGSQARILVTAKYP
CCCHHHHCHHHHHHHHHHHCCCCCHHEEEEHHHHHHHHHHHHHHHHCCCCCEEEEEECCC
FQARAAERLGADLVLLGRTGPALYREIARLTGARVLRARFGRWLVQGGVDQVFDCVGSSR
CHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
SVHDALTLTRAGGRVVLVGLASDPRNVDWTPIWLREVEIRGSMAYAEEELDGIRASSIAH
CHHHHHEEEECCCEEEEEEECCCCCCCCCCEEEEEEEEECCCHHHHHHHHCCHHHHHHHH
GVRLMAERRVELGWLVTHRFALAEYQEALATVTGKGANGVIKAVFAFSGGNRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]