Click here to switch to the map view.

The map label for this gene is hypF [H]

Identifier: 221633836

GI number: 221633836

Start: 1874536

End: 1876812

Strand: Reverse

Name: hypF [H]

Synonym: trd_1863

Alternate gene names: 221633836

Gene position: 1876812-1874536 (Counterclockwise)

Preceding gene: 221633837

Following gene: 221633835

Centisome position: 93.7

GC content: 64.82

Gene sequence:

>2277_bases
GTGGCTGCACGATCTGAAAGATCACGCATCCTGGCCTGGTCCATTCGTGTCACCGGTGTCGTCCAGGGAGTCGGCTTCCG
GCCCTTCGTCTATCGCCTGGCGACGAGGCTCGGGTTAGGGGGATGGGTGCTCAATACGGAACAGGGCGTCGAGATCGTAG
TCGAGGGACCCGAGCCCGCGCTCGCGACCTTCCTGTCCGAGCTGGAGTCCAACCCACCGCCAGCCGCCCGCATCGCGAGC
CTGAGTGTCCAGCAGACTCGGCCGATCGGCTTCGCACAGTTCACGATCCGCGAGAGCGCCCAAGGTGACCGACCGACGAC
TCGCGTGTCGCCTGACCTAGCGACATGCGACGCGTGCCTGCGCGAGCTGTTCGACCCAGCCGATCGGCGCTTCCACTACC
CGTATATCAATTGCACGAACTGCGGGCCCCGCTACAGTATCATCCTCTCCTTACCCTATGACCGGCCACGCACGACCATG
AAGGATTGGTCCCTCTGCCCTGATTGTGACCGGGAATACCACGATCCTGGAGATCGGCGCTTCCACGCCCAGCCGATCGC
CTGCCCGCTCTGTGGACCGCAGTACCAACTGTGCACAGCAACGGGCCAGCTCGTCGCCCGAGGAGAGGATGCCGTTCGTC
TGGCGGCTAGCTGGTTGGTTGCTGGGAAAATTCTCGCTGTGAAAGGGATCGGCGGCTACCACCTAGCGTGTGACGCACGG
AATGCCGCTGCGGTCTGGGCGCTCCGCGAGCGGAAGTTCCGCAAGGAGAAACCGTTCGCCTTGATGGTTCGAGATCTCGA
TACCGCGGCCCAGCTCGTCGAGCTGGACGACGCAGCCAGCGCCCTCCTCGGGAGTCCAGCTCGCCCCATCCTCCTTCTGC
CGGCCAGGATCGAGCTGCCAGGTGTTGCGCCCGAGAATCGCGACTTTGGAATCATGCTGCCGTACGCTCCCTTGCACTAC
TTGCTCTTTGCCTTCGGTGCTCCCGCTGTTCTCGTCATGACCAGCGCGAACCGCTCAGACGAGCCGATCGCCTTCTGCGA
CGACGACGCGTTCGCTCGTCTGAGCGGTATCGCCGACGCGTTCCTGGTTGGAGAGCGGCCGATCGCCCGAAGGATAGACG
ACTCGGTTATACGCCACGGTCCGAGCGGATCCATCCTGGTTCGCCGTGCGCGCGGCCTTGCACCAGCCGTCGTCGCCACA
CTCCCGACCACGCGACCGATCCTCGCTGTCGGTGCAGACCTCAAGAACACGGTAACGCTCGTCGTCGCTGGCCAGGCATT
CGTGAGCCAGCACATCGGTGACCTCGAGCATTGGCCGGTCGTCGAGGCATTTTCGACCACCATCCGCGACCTTTGCGCCA
TGTACGAGGTCAACCTCGATGAGGCGCTGATCGTCCACGATGCGCACCCGGAGTATGTCTCCACTCGACTGGCACAGGAG
TTGCCCGGCACGCACGTCGCGGTGCAGCACCACCGCGCCCATATCGCCAGCGTTCTGGCTGAGCATGAATGCTGGGACGA
TTCCGTAGTCGGCTTCGCCTTCGATGGTACTGGATACGGCGACGACAGCAGTATCTGGGGTGGCGAGGTCTTCGTGGGAA
GCCTGGTCGGCGGGCTGCGGCGTGTGGCATCGCTGCGGCCAGTGCTCCTGCCTGGTGGAGATGCAGCCGCACGATGGCCC
GTGCAAGCAGCGGCCGGATTCTTGGCCATGCTCCCCGAGCTTCCCGATCTGCACGCCTCCCCCTTCGCGTTCCCGAAGCG
GTACGACGAGGCCTGCACAGTACTGCGACGCCGGGTGCGCTGCCACCCCACGACGTCAATGGGGCGACTCTTCGACACGG
TTGCCGCTCTCTGTGGCTTCACGCGACCGATCACCTTCGAGGGGCAGGCAGCGATCTGGTTGGAGCATCTGGCCCGCCAG
GCGGGTGAGGTCGCCCCGTACCCCTTCCCGTTCGTCAACCACGAACTTGACTTCCGGCCGCTCCTCGCGGCAGTCATTCG
CGATCGCCAGCGCGGGCGCGACATCGCTGCCATCGCTCGCGCCTTCCACGAGGCGGTCGCCCACGCGATCTGCTCGGTGG
CTGCGTATTGCCGAGAAGCCTACGGGATACGCCACGTCGTCCTCTCAGGAGGAGTTTTCCAAAACATGCTGCTCCTGACG
CGCGTGCAGCAGCTTCTCGGCCAGACGACGGTCTGGGTGAACCGGATGGTTCCGCCCAACGACGGCGGGATCAGTCTGGG
GCAGGCAGCCCTCGCTGCTGCGCGCGACGCGTCGTAA

Upstream 100 bases:

>100_bases
GTCGATGCACGCCAGCGTCTCCTCCAACTCACGGGTGGCAGGCGAACGATCCCGGTCCTGGTCGAGGATGGCCGGGTAGT
CTTGATCGGGTGGCAGGGTC

Downstream 100 bases:

>100_bases
GGTCTCTCGCTCGCGCTGCGAGCCGGTTGCCGCAAAGCGACCCCGTGCACTGCACAGTCTCGCGCCCGCTCGATGGGCAG
GTGGACAGCGAACGAGCCTC

Product: [NiFe] hydrogenase maturation protein HypF

Products: NA

Alternate protein names: Carbamoyl phosphate-converting enzyme hypF; [NiFe]-hydrogenase maturation factor hypF; Hydrogenase maturation protein hypF [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MAARSERSRILAWSIRVTGVVQGVGFRPFVYRLATRLGLGGWVLNTEQGVEIVVEGPEPALATFLSELESNPPPAARIAS
LSVQQTRPIGFAQFTIRESAQGDRPTTRVSPDLATCDACLRELFDPADRRFHYPYINCTNCGPRYSIILSLPYDRPRTTM
KDWSLCPDCDREYHDPGDRRFHAQPIACPLCGPQYQLCTATGQLVARGEDAVRLAASWLVAGKILAVKGIGGYHLACDAR
NAAAVWALRERKFRKEKPFALMVRDLDTAAQLVELDDAASALLGSPARPILLLPARIELPGVAPENRDFGIMLPYAPLHY
LLFAFGAPAVLVMTSANRSDEPIAFCDDDAFARLSGIADAFLVGERPIARRIDDSVIRHGPSGSILVRRARGLAPAVVAT
LPTTRPILAVGADLKNTVTLVVAGQAFVSQHIGDLEHWPVVEAFSTTIRDLCAMYEVNLDEALIVHDAHPEYVSTRLAQE
LPGTHVAVQHHRAHIASVLAEHECWDDSVVGFAFDGTGYGDDSSIWGGEVFVGSLVGGLRRVASLRPVLLPGGDAAARWP
VQAAAGFLAMLPELPDLHASPFAFPKRYDEACTVLRRRVRCHPTTSMGRLFDTVAALCGFTRPITFEGQAAIWLEHLARQ
AGEVAPYPFPFVNHELDFRPLLAAVIRDRQRGRDIAAIARAFHEAVAHAICSVAAYCREAYGIRHVVLSGGVFQNMLLLT
RVQQLLGQTTVWVNRMVPPNDGGISLGQAALAAARDAS

Sequences:

>Translated_758_residues
MAARSERSRILAWSIRVTGVVQGVGFRPFVYRLATRLGLGGWVLNTEQGVEIVVEGPEPALATFLSELESNPPPAARIAS
LSVQQTRPIGFAQFTIRESAQGDRPTTRVSPDLATCDACLRELFDPADRRFHYPYINCTNCGPRYSIILSLPYDRPRTTM
KDWSLCPDCDREYHDPGDRRFHAQPIACPLCGPQYQLCTATGQLVARGEDAVRLAASWLVAGKILAVKGIGGYHLACDAR
NAAAVWALRERKFRKEKPFALMVRDLDTAAQLVELDDAASALLGSPARPILLLPARIELPGVAPENRDFGIMLPYAPLHY
LLFAFGAPAVLVMTSANRSDEPIAFCDDDAFARLSGIADAFLVGERPIARRIDDSVIRHGPSGSILVRRARGLAPAVVAT
LPTTRPILAVGADLKNTVTLVVAGQAFVSQHIGDLEHWPVVEAFSTTIRDLCAMYEVNLDEALIVHDAHPEYVSTRLAQE
LPGTHVAVQHHRAHIASVLAEHECWDDSVVGFAFDGTGYGDDSSIWGGEVFVGSLVGGLRRVASLRPVLLPGGDAAARWP
VQAAAGFLAMLPELPDLHASPFAFPKRYDEACTVLRRRVRCHPTTSMGRLFDTVAALCGFTRPITFEGQAAIWLEHLARQ
AGEVAPYPFPFVNHELDFRPLLAAVIRDRQRGRDIAAIARAFHEAVAHAICSVAAYCREAYGIRHVVLSGGVFQNMLLLT
RVQQLLGQTTVWVNRMVPPNDGGISLGQAALAAARDAS
>Mature_757_residues
AARSERSRILAWSIRVTGVVQGVGFRPFVYRLATRLGLGGWVLNTEQGVEIVVEGPEPALATFLSELESNPPPAARIASL
SVQQTRPIGFAQFTIRESAQGDRPTTRVSPDLATCDACLRELFDPADRRFHYPYINCTNCGPRYSIILSLPYDRPRTTMK
DWSLCPDCDREYHDPGDRRFHAQPIACPLCGPQYQLCTATGQLVARGEDAVRLAASWLVAGKILAVKGIGGYHLACDARN
AAAVWALRERKFRKEKPFALMVRDLDTAAQLVELDDAASALLGSPARPILLLPARIELPGVAPENRDFGIMLPYAPLHYL
LFAFGAPAVLVMTSANRSDEPIAFCDDDAFARLSGIADAFLVGERPIARRIDDSVIRHGPSGSILVRRARGLAPAVVATL
PTTRPILAVGADLKNTVTLVVAGQAFVSQHIGDLEHWPVVEAFSTTIRDLCAMYEVNLDEALIVHDAHPEYVSTRLAQEL
PGTHVAVQHHRAHIASVLAEHECWDDSVVGFAFDGTGYGDDSSIWGGEVFVGSLVGGLRRVASLRPVLLPGGDAAARWPV
QAAAGFLAMLPELPDLHASPFAFPKRYDEACTVLRRRVRCHPTTSMGRLFDTVAALCGFTRPITFEGQAAIWLEHLARQA
GEVAPYPFPFVNHELDFRPLLAAVIRDRQRGRDIAAIARAFHEAVAHAICSVAAYCREAYGIRHVVLSGGVFQNMLLLTR
VQQLLGQTTVWVNRMVPPNDGGISLGQAALAAARDAS

Specific function: Along with hypE, it catalyzes the synthesis of the CN ligands of the active site iron of [NiFe]-hydrogenases using carbamoylphosphate as a substrate. It functions as a carbamoyl transferase using carbamoylphosphate as a substrate and transferring the carb

COG id: COG0068

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 YrdC-like domain [H]

Homologues:

Organism=Escherichia coli, GI2367152, Length=768, Percent_Identity=41.1458333333333, Blast_Score=438, Evalue=1e-124,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR017968
- InterPro:   IPR004421
- InterPro:   IPR017945
- InterPro:   IPR006070
- InterPro:   IPR011125 [H]

Pfam domain/function: PF00708 Acylphosphatase; PF01300 Sua5_yciO_yrdC; PF07503 zf-HYPF [H]

EC number: NA

Molecular weight: Translated: 82541; Mature: 82409

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00150 ACYLPHOSPHATASE_1 ; PS51160 ACYLPHOSPHATASE_3 ; PS51163 YRDC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAARSERSRILAWSIRVTGVVQGVGFRPFVYRLATRLGLGGWVLNTEQGVEIVVEGPEPA
CCCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHH
LATFLSELESNPPPAARIASLSVQQTRPIGFAQFTIRESAQGDRPTTRVSPDLATCDACL
HHHHHHHHHCCCCCHHHHHHCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
RELFDPADRRFHYPYINCTNCGPRYSIILSLPYDRPRTTMKDWSLCPDCDREYHDPGDRR
HHHHCHHHCCEECCEEEECCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCE
FHAQPIACPLCGPQYQLCTATGQLVARGEDAVRLAASWLVAGKILAVKGIGGYHLACDAR
EECCCCCCCCCCCCCEEEHHCCHHEECCCHHHHHHHHHHHHCEEEEEECCCCEEEEECCC
NAAAVWALRERKFRKEKPFALMVRDLDTAAQLVELDDAASALLGSPARPILLLPARIELP
CHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEECEEECC
GVAPENRDFGIMLPYAPLHYLLFAFGAPAVLVMTSANRSDEPIAFCDDDAFARLSGIADA
CCCCCCCCCEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHH
FLVGERPIARRIDDSVIRHGPSGSILVRRARGLAPAVVATLPTTRPILAVGADLKNTVTL
HHCCCCHHHHHHHHHHHHCCCCCCEEEEHHCCCCCHHHHHCCCCCCEEEECCCCCCEEEE
VVAGQAFVSQHIGDLEHWPVVEAFSTTIRDLCAMYEVNLDEALIVHDAHPEYVSTRLAQE
EEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
LPGTHVAVQHHRAHIASVLAEHECWDDSVVGFAFDGTGYGDDSSIWGGEVFVGSLVGGLR
CCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RVASLRPVLLPGGDAAARWPVQAAAGFLAMLPELPDLHASPFAFPKRYDEACTVLRRRVR
HHHHCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
CHPTTSMGRLFDTVAALCGFTRPITFEGQAAIWLEHLARQAGEVAPYPFPFVNHELDFRP
CCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LLAAVIRDRQRGRDIAAIARAFHEAVAHAICSVAAYCREAYGIRHVVLSGGVFQNMLLLT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHH
RVQQLLGQTTVWVNRMVPPNDGGISLGQAALAAARDAS
HHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
AARSERSRILAWSIRVTGVVQGVGFRPFVYRLATRLGLGGWVLNTEQGVEIVVEGPEPA
CCCCCCCEEEEEEEEEEEEEECCCCHHHHHHHHHHHCCCCEEEECCCCCEEEEECCCHH
LATFLSELESNPPPAARIASLSVQQTRPIGFAQFTIRESAQGDRPTTRVSPDLATCDACL
HHHHHHHHHCCCCCHHHHHHCCHHHCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHHHH
RELFDPADRRFHYPYINCTNCGPRYSIILSLPYDRPRTTMKDWSLCPDCDREYHDPGDRR
HHHHCHHHCCEECCEEEECCCCCCEEEEEECCCCCCCCCHHCCCCCCCCCCCCCCCCCCE
FHAQPIACPLCGPQYQLCTATGQLVARGEDAVRLAASWLVAGKILAVKGIGGYHLACDAR
EECCCCCCCCCCCCCEEEHHCCHHEECCCHHHHHHHHHHHHCEEEEEECCCCEEEEECCC
NAAAVWALRERKFRKEKPFALMVRDLDTAAQLVELDDAASALLGSPARPILLLPARIELP
CHHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHCCCCCCEEEEECEEECC
GVAPENRDFGIMLPYAPLHYLLFAFGAPAVLVMTSANRSDEPIAFCDDDAFARLSGIADA
CCCCCCCCCEEECCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEECCHHHHHHHHHHHH
FLVGERPIARRIDDSVIRHGPSGSILVRRARGLAPAVVATLPTTRPILAVGADLKNTVTL
HHCCCCHHHHHHHHHHHHCCCCCCEEEEHHCCCCCHHHHHCCCCCCEEEECCCCCCEEEE
VVAGQAFVSQHIGDLEHWPVVEAFSTTIRDLCAMYEVNLDEALIVHDAHPEYVSTRLAQE
EEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHH
LPGTHVAVQHHRAHIASVLAEHECWDDSVVGFAFDGTGYGDDSSIWGGEVFVGSLVGGLR
CCCCEEEHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHH
RVASLRPVLLPGGDAAARWPVQAAAGFLAMLPELPDLHASPFAFPKRYDEACTVLRRRVR
HHHHCCCEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
CHPTTSMGRLFDTVAALCGFTRPITFEGQAAIWLEHLARQAGEVAPYPFPFVNHELDFRP
CCCCHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHH
LLAAVIRDRQRGRDIAAIARAFHEAVAHAICSVAAYCREAYGIRHVVLSGGVFQNMLLLT
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHH
RVQQLLGQTTVWVNRMVPPNDGGISLGQAALAAARDAS
HHHHHHCCCEEEEECCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]