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The map label for this gene is alaXM [H]

Identifier: 221633766

GI number: 221633766

Start: 1797942

End: 1798697

Strand: Reverse

Name: alaXM [H]

Synonym: trd_1793

Alternate gene names: 221633766

Gene position: 1798697-1797942 (Counterclockwise)

Preceding gene: 221633767

Following gene: 221633765

Centisome position: 89.8

GC content: 62.96

Gene sequence:

>756_bases
ATGCCGACGGAACTTTTGTACCTGAACGACAGCTACGTCCGCGAGTTCACGGCGACCGTGCTCGCGGTGACGCCGGATCA
GGGTGTCATCCTGGATCGCACCGCGTTCTATCCCGGTGGTGGTGGACAGCCACACGACACTGGTGAGCTGGTGAGCACCG
ATGGTCGATCGTGGCGAGTGGTCTCGCTCCGACGCGGTACGACTGCGGATGAGATCGTGCATCTCCTCGATCGCGAACCA
CCTCCGGTGGGTACGGCCGTGCGGGGTGTGCTGGATTGGGACCGACGCTACCGGCTGATGCGCTTCCACACCGCCTTGCA
CGTTCTTTCAGCAGTGGTCTTCCGCGAGTTCGGCGCTCTGGTCACCGGTGCGAACCTGGATGTGGACAAAGCGCGGATGG
ACTTCGAACTCGAGGATCTGGCCCCGGAACGAGTCGCACGCATCGAACAGCTGGCCAACGAGGCGATTGCCCGTGGACTC
CCTGTCCGCTGGTGGACGATTCCGCGGGCTGAGGCAGAGCGGATACCGGACTTGATCCGAACCAAGGTCAACTTACTCCC
GCCGAGCATCCAGTCGATCCGCGTCGTCGAGATCAGCGGGCTCGATCTCCAGGCAGATGGCGGAACGCACGTCCGCAATA
CGCGGGAAATCGGCGGTATCCGCGTCGTCGGTACCCGCTCGAAAGGACGCAACAACAAGCGGATCGAGATCACCCTCGTC
GATCAGGCCGAGAACGAGCGGACGGAGGGATCGTGA

Upstream 100 bases:

>100_bases
CCGTCGCCGGGTCGACGGTCGTACACCATGCGGGTGCAGCGCCGGCCGTTTGACGAGGCCGCTCGTGGACCCTATCATCG
CGGCAGGGAGGCGAGTCGGG

Downstream 100 bases:

>100_bases
GGATCGATTGGTCGACCATTTTGGCCGGGCGGGCCAGAGCGTTCCGTCCCTGCATCCGGACGGACTTTTCCTCGCTCCCC
ACACCACCCGATGTCATCCA

Product: alanyl-tRNA synthetase

Products: AMP; diphosphate; L-alanyl-tRNA(Ala)

Alternate protein names: AlaX-M; Alanyl-tRNA deacylase AlaX-M [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MPTELLYLNDSYVREFTATVLAVTPDQGVILDRTAFYPGGGGQPHDTGELVSTDGRSWRVVSLRRGTTADEIVHLLDREP
PPVGTAVRGVLDWDRRYRLMRFHTALHVLSAVVFREFGALVTGANLDVDKARMDFELEDLAPERVARIEQLANEAIARGL
PVRWWTIPRAEAERIPDLIRTKVNLLPPSIQSIRVVEISGLDLQADGGTHVRNTREIGGIRVVGTRSKGRNNKRIEITLV
DQAENERTEGS

Sequences:

>Translated_251_residues
MPTELLYLNDSYVREFTATVLAVTPDQGVILDRTAFYPGGGGQPHDTGELVSTDGRSWRVVSLRRGTTADEIVHLLDREP
PPVGTAVRGVLDWDRRYRLMRFHTALHVLSAVVFREFGALVTGANLDVDKARMDFELEDLAPERVARIEQLANEAIARGL
PVRWWTIPRAEAERIPDLIRTKVNLLPPSIQSIRVVEISGLDLQADGGTHVRNTREIGGIRVVGTRSKGRNNKRIEITLV
DQAENERTEGS
>Mature_250_residues
PTELLYLNDSYVREFTATVLAVTPDQGVILDRTAFYPGGGGQPHDTGELVSTDGRSWRVVSLRRGTTADEIVHLLDREPP
PVGTAVRGVLDWDRRYRLMRFHTALHVLSAVVFREFGALVTGANLDVDKARMDFELEDLAPERVARIEQLANEAIARGLP
VRWWTIPRAEAERIPDLIRTKVNLLPPSIQSIRVVEISGLDLQADGGTHVRNTREIGGIRVVGTRSKGRNNKRIEITLVD
QAENERTEGS

Specific function: Functions in trans to edit the amino acid moiety from incorrectly charged Ser-tRNA(Ala) or Gly-tRNA(Ala). Has no activity on incorrectly charged Ser-tRNA(Thr), nor on correctly charged Ala-tRNA(Ala) or Ser-tRNA(Ser) [H]

COG id: COG2872

COG function: function code R; Predicted metal-dependent hydrolases related to alanyl-tRNA synthetase HxxxH domain

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Editing domain AlaX-M subfamily [H]

Homologues:

Organism=Homo sapiens, GI13376886, Length=237, Percent_Identity=30.8016877637131, Blast_Score=89, Evalue=4e-18,
Organism=Homo sapiens, GI217416402, Length=237, Percent_Identity=30.8016877637131, Blast_Score=89, Evalue=5e-18,
Organism=Escherichia coli, GI1789048, Length=213, Percent_Identity=29.1079812206573, Blast_Score=71, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6324911, Length=244, Percent_Identity=27.0491803278689, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: 6.1.1.7

Molecular weight: Translated: 28006; Mature: 27875

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTELLYLNDSYVREFTATVLAVTPDQGVILDRTAFYPGGGGQPHDTGELVSTDGRSWRV
CCCEEEEECHHHHHHHEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCEEE
VSLRRGTTADEIVHLLDREPPPVGTAVRGVLDWDRRYRLMRFHTALHVLSAVVFREFGAL
EEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VTGANLDVDKARMDFELEDLAPERVARIEQLANEAIARGLPVRWWTIPRAEAERIPDLIR
EECCCCCHHHHHCCEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCHHHHH
TKVNLLPPSIQSIRVVEISGLDLQADGGTHVRNTREIGGIRVVGTRSKGRNNKRIEITLV
HHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
DQAENERTEGS
ECCCCCCCCCC
>Mature Secondary Structure 
PTELLYLNDSYVREFTATVLAVTPDQGVILDRTAFYPGGGGQPHDTGELVSTDGRSWRV
CCEEEEECHHHHHHHEEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCEEECCCCCEEE
VSLRRGTTADEIVHLLDREPPPVGTAVRGVLDWDRRYRLMRFHTALHVLSAVVFREFGAL
EEEECCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE
VTGANLDVDKARMDFELEDLAPERVARIEQLANEAIARGLPVRWWTIPRAEAERIPDLIR
EECCCCCHHHHHCCEEHHHCCHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHCHHHHH
TKVNLLPPSIQSIRVVEISGLDLQADGGTHVRNTREIGGIRVVGTRSKGRNNKRIEITLV
HHHHCCCCCCCCEEEEEEECCEEECCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEEEEE
DQAENERTEGS
ECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-alanine; tRNA(Ala)

Specific reaction: ATP + L-alanine + tRNA(Ala) = AMP + diphosphate + L-alanyl-tRNA(Ala)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA