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The map label for this gene is algU [H]

Identifier: 221633725

GI number: 221633725

Start: 1758981

End: 1759604

Strand: Reverse

Name: algU [H]

Synonym: trd_1752

Alternate gene names: 221633725

Gene position: 1759604-1758981 (Counterclockwise)

Preceding gene: 221633728

Following gene: 221633724

Centisome position: 87.85

GC content: 66.51

Gene sequence:

>624_bases
ATGTCTGGCAGCGAAGACGAGGCGTTTCTGGTAGCTCGGGCACGGACCGGCGATCGCGATGCGTTCGCCGAACTCGTCCG
TCGCTACCAGCGACTCGTCTTCGCCGTCTCCTATCGCTTGCTCGGCGATCCTGACCTCGCTGAAGACGTGGCGCAGGAGA
CCTTCATTCGGGCCTTCCTCTCGCTGGAGCACTTCCGCGGCACGACGATGCGTGCCTGGCTCCTGCGGATCGCGCACAAT
GCTGCCCTCGATCAGTTGCGGGCGAGTGCGCGACGTCGCACCGTCCCACTCGAACACGCCGGCGAGCATGCTTCTTTCGA
TGTCCCCGAGCCAGGTATCGAACAGGCGGGACTCCGCGCGGCGTTGGAAGCCGCCTTGGCCGCGCTCCCCGTCGAGCAAC
GAGCGGTCGTCGTGCTCGCCGATGTCGAGGGGTTGCCCTACGAGGAGATCGCGCAGACACTCGGCCTTCCGCTCGGAACC
GTCAAGTCGCGCCTGGCGCGTGCCCGCATGCGTCTGCGCACGCTGCTGGAGTCCGATCCGCGAGCGAGGGAACTCTTGGG
GCTGAGCGGTCGTCTCCCGGATAGGGAGGGTGAGTCGCCGGAACGCCTGATCGAAGGTGAGTGA

Upstream 100 bases:

>100_bases
CCGGGCCCTTGCGCACGAGGAGCGACTGCGGTACGGGGTCGGGGTGGACGAACGACCGTCGCCTCGAGTACCGTTCCGGC
AAAGGGACCGGTGGTTCGGC

Downstream 100 bases:

>100_bases
CGGTTCTCGATGACCGATCGCAGCAGTCACTTGGACGAAGAGCGACTCGCGCTCCTGGCGAGCGAGGAGCCGGATGCGGA
GGTATCGGCGCATCTCGCCC

Product: RNA polymerase sigma-H factor

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSGSEDEAFLVARARTGDRDAFAELVRRYQRLVFAVSYRLLGDPDLAEDVAQETFIRAFLSLEHFRGTTMRAWLLRIAHN
AALDQLRASARRRTVPLEHAGEHASFDVPEPGIEQAGLRAALEAALAALPVEQRAVVVLADVEGLPYEEIAQTLGLPLGT
VKSRLARARMRLRTLLESDPRARELLGLSGRLPDREGESPERLIEGE

Sequences:

>Translated_207_residues
MSGSEDEAFLVARARTGDRDAFAELVRRYQRLVFAVSYRLLGDPDLAEDVAQETFIRAFLSLEHFRGTTMRAWLLRIAHN
AALDQLRASARRRTVPLEHAGEHASFDVPEPGIEQAGLRAALEAALAALPVEQRAVVVLADVEGLPYEEIAQTLGLPLGT
VKSRLARARMRLRTLLESDPRARELLGLSGRLPDREGESPERLIEGE
>Mature_206_residues
SGSEDEAFLVARARTGDRDAFAELVRRYQRLVFAVSYRLLGDPDLAEDVAQETFIRAFLSLEHFRGTTMRAWLLRIAHNA
ALDQLRASARRRTVPLEHAGEHASFDVPEPGIEQAGLRAALEAALAALPVEQRAVVVLADVEGLPYEEIAQTLGLPLGTV
KSRLARARMRLRTLLESDPRARELLGLSGRLPDREGESPERLIEGE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=179, Percent_Identity=36.3128491620112, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22945; Mature: 22814

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: PS00622 HTH_LUXR_1 ; PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGSEDEAFLVARARTGDRDAFAELVRRYQRLVFAVSYRLLGDPDLAEDVAQETFIRAFL
CCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SLEHFRGTTMRAWLLRIAHNAALDQLRASARRRTVPLEHAGEHASFDVPEPGIEQAGLRA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHH
ALEAALAALPVEQRAVVVLADVEGLPYEEIAQTLGLPLGTVKSRLARARMRLRTLLESDP
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
RARELLGLSGRLPDREGESPERLIEGE
HHHHHHCCCCCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
SGSEDEAFLVARARTGDRDAFAELVRRYQRLVFAVSYRLLGDPDLAEDVAQETFIRAFL
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
SLEHFRGTTMRAWLLRIAHNAALDQLRASARRRTVPLEHAGEHASFDVPEPGIEQAGLRA
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCHHHHHHHH
ALEAALAALPVEQRAVVVLADVEGLPYEEIAQTLGLPLGTVKSRLARARMRLRTLLESDP
HHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCC
RARELLGLSGRLPDREGESPERLIEGE
HHHHHHCCCCCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]