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The map label for this gene is pyk [H]

Identifier: 221633701

GI number: 221633701

Start: 1732620

End: 1734059

Strand: Reverse

Name: pyk [H]

Synonym: trd_1728

Alternate gene names: 221633701

Gene position: 1734059-1732620 (Counterclockwise)

Preceding gene: 221633705

Following gene: 221633700

Centisome position: 86.57

GC content: 65.62

Gene sequence:

>1440_bases
GTGGCGGAAGAGCGAGCGTCGTGCTGGCGACGTCGCGCCAAGATCGTGGCAACGATCGGTCCGGCCTCCTGGAGTCCGCC
GGTTTTGGACCAGCTGATCGCCGCTGGTGTCGATGTCTTTCGGGTGAACGCTGCGCACAACTCGCCGGAGGAACGCCGGC
CTATCGTGGCTGACCTGCGAGCTGCTGCTGACCGGGCAGGGCGACCGATCGCCATCCTGCAGGATTTGGCGAGTTGGAAG
CCACGGACTGGTCCCCTGGAGGGTGATTCATTCCGCCTGATTCGCGACACGCGTGTGCGGATCGTTCCGGGCAGTGAGCC
GCTCTCGCCGGAGCGGCTCAGTATCGACGATGCAGAACTGGTCGCTCAGCTGCGGCCCGGGCATCGGATCCTGATCAGCG
ATGGGTTGATCGAGCTCGTCGTCGAGTCGCGCGCGGGAGACGAGCTGATCGCTCGCGTCCTGCGTGGTGGCCAGATCCGG
GGGCGACAGGGCGTTGCCGTACCAGGGGTCCTCGGGCGTCCTTTCCGATTGAGCGAACGCGATCGAGCCGATATCGCCTT
CGCCGCCGAGCACGAGCTGGAGTACATCGGGGTGAGCTTCGTCACGGGGCCGGATGATCTCCGGTCGGTGCGAGCGATCC
TGCGCGAGTGTGGCGGGCGCGCTCGGCTCGTCGCGAAAATCGAGCGTCCCGAGGCGCTGGCAGCGATCCGCGAGATCGCG
CGCGAGAGCGACGCTGTCATGGTCGCTCGGGGCGATCTCGGTGTTCAGCTGCCGCCGGAGGAAGTGCCCATCGCGCAAAA
GCGGATCATCCGCGTTGCCCGTGCCTTCGGTATTCCCAGCATTATCGCCACACAGATGCTGGAATCGATGACGACGCAGC
CGATCCCGACTCGCGCGGAGGTCAGCGACGTGGCGAATGCTGTTCTCGAGGGAGCTGACGCTCTGATGCTCAGTGCGGAA
ACCGCGACTGGTCAGTATCCGGTCGAGGCGGTCGAGATGATGCATCGAATCATCGTCGCCACCGAGCAGCAGCTGACCCC
TGCACCGGTTCCCGAAGAGGAAGAGCCGACGACGATCGCTGCGACGATCGCGCGCACCGCCACCGATCTCGCTCGACGTT
GGCGAGCGGTTCGCACGATCGTGGCGATCACGCGAAGCGGGTTTACCGCTCGGGAAGTGGCCCGCGAGCGACCGGTCGCA
CCGATCGTCGCGGTGACCTGTGATCCCATGGTTGCCCGGCAACTCTCCCTGGTTTGGGGGATTGTGCCGCTCGCACTTCC
TTCGTTCGCGGAAACCAGCGAGGAGTTGATCGAGCAGGCAGCAGGGGCAGCGCGTGCGAGCGGGCTCGTCAGGGGCGGTG
ATCACGCCGTCTTCATTACGAGTCTCCGCTATTTTCCCGAGCCCGGGCACGCCGATACGCTCCATCTCCGCCAACTCTGA

Upstream 100 bases:

>100_bases
GATCGAGAGCGGTGCCGGAGAGCGCGCATTGCCTGCTAGCGTCTTGCGGGCTATCCTCGTTTGCTCGTCGTGTCGGGCCG
GGGGAGCGAGGAGGTGTCTC

Downstream 100 bases:

>100_bases
ACCCAGGGGCTGCTGCCCCGCGAGCAGCTCTGATGGTCGCGCTGCTCTCGCTGTGTGCCGAGAAGGCTGGGTATCGGCCG
AGTGATCCTGCCAGAGCATG

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 479; Mature: 478

Protein sequence:

>479_residues
MAEERASCWRRRAKIVATIGPASWSPPVLDQLIAAGVDVFRVNAAHNSPEERRPIVADLRAAADRAGRPIAILQDLASWK
PRTGPLEGDSFRLIRDTRVRIVPGSEPLSPERLSIDDAELVAQLRPGHRILISDGLIELVVESRAGDELIARVLRGGQIR
GRQGVAVPGVLGRPFRLSERDRADIAFAAEHELEYIGVSFVTGPDDLRSVRAILRECGGRARLVAKIERPEALAAIREIA
RESDAVMVARGDLGVQLPPEEVPIAQKRIIRVARAFGIPSIIATQMLESMTTQPIPTRAEVSDVANAVLEGADALMLSAE
TATGQYPVEAVEMMHRIIVATEQQLTPAPVPEEEEPTTIAATIARTATDLARRWRAVRTIVAITRSGFTAREVARERPVA
PIVAVTCDPMVARQLSLVWGIVPLALPSFAETSEELIEQAAGAARASGLVRGGDHAVFITSLRYFPEPGHADTLHLRQL

Sequences:

>Translated_479_residues
MAEERASCWRRRAKIVATIGPASWSPPVLDQLIAAGVDVFRVNAAHNSPEERRPIVADLRAAADRAGRPIAILQDLASWK
PRTGPLEGDSFRLIRDTRVRIVPGSEPLSPERLSIDDAELVAQLRPGHRILISDGLIELVVESRAGDELIARVLRGGQIR
GRQGVAVPGVLGRPFRLSERDRADIAFAAEHELEYIGVSFVTGPDDLRSVRAILRECGGRARLVAKIERPEALAAIREIA
RESDAVMVARGDLGVQLPPEEVPIAQKRIIRVARAFGIPSIIATQMLESMTTQPIPTRAEVSDVANAVLEGADALMLSAE
TATGQYPVEAVEMMHRIIVATEQQLTPAPVPEEEEPTTIAATIARTATDLARRWRAVRTIVAITRSGFTAREVARERPVA
PIVAVTCDPMVARQLSLVWGIVPLALPSFAETSEELIEQAAGAARASGLVRGGDHAVFITSLRYFPEPGHADTLHLRQL
>Mature_478_residues
AEERASCWRRRAKIVATIGPASWSPPVLDQLIAAGVDVFRVNAAHNSPEERRPIVADLRAAADRAGRPIAILQDLASWKP
RTGPLEGDSFRLIRDTRVRIVPGSEPLSPERLSIDDAELVAQLRPGHRILISDGLIELVVESRAGDELIARVLRGGQIRG
RQGVAVPGVLGRPFRLSERDRADIAFAAEHELEYIGVSFVTGPDDLRSVRAILRECGGRARLVAKIERPEALAAIREIAR
ESDAVMVARGDLGVQLPPEEVPIAQKRIIRVARAFGIPSIIATQMLESMTTQPIPTRAEVSDVANAVLEGADALMLSAET
ATGQYPVEAVEMMHRIIVATEQQLTPAPVPEEEEPTTIAATIARTATDLARRWRAVRTIVAITRSGFTAREVARERPVAP
IVAVTCDPMVARQLSLVWGIVPLALPSFAETSEELIEQAAGAARASGLVRGGDHAVFITSLRYFPEPGHADTLHLRQL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=486, Percent_Identity=35.3909465020576, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI33286420, Length=486, Percent_Identity=35.3909465020576, Blast_Score=269, Evalue=5e-72,
Organism=Homo sapiens, GI33286418, Length=485, Percent_Identity=35.4639175257732, Blast_Score=268, Evalue=9e-72,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=36.2903225806452, Blast_Score=253, Evalue=2e-67,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=36.2903225806452, Blast_Score=253, Evalue=3e-67,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=32.6388888888889, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=32.6388888888889, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI310128736, Length=235, Percent_Identity=28.5106382978723, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI310128734, Length=235, Percent_Identity=28.5106382978723, Blast_Score=109, Evalue=6e-24,
Organism=Homo sapiens, GI310128738, Length=214, Percent_Identity=26.6355140186916, Blast_Score=91, Evalue=3e-18,
Organism=Escherichia coli, GI1787965, Length=462, Percent_Identity=35.0649350649351, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1788160, Length=470, Percent_Identity=36.8085106382979, Blast_Score=241, Evalue=6e-65,
Organism=Caenorhabditis elegans, GI17544584, Length=468, Percent_Identity=36.3247863247863, Blast_Score=269, Evalue=3e-72,
Organism=Caenorhabditis elegans, GI71984406, Length=471, Percent_Identity=37.5796178343949, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=471, Percent_Identity=37.5796178343949, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=471, Percent_Identity=37.5796178343949, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI71984413, Length=471, Percent_Identity=37.5796178343949, Blast_Score=259, Evalue=3e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=32.7835051546392, Blast_Score=242, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6324923, Length=484, Percent_Identity=31.6115702479339, Blast_Score=211, Evalue=3e-55,
Organism=Drosophila melanogaster, GI24648964, Length=476, Percent_Identity=35.9243697478992, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI28571814, Length=476, Percent_Identity=35.9243697478992, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=37.1007371007371, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24581235, Length=472, Percent_Identity=30.5084745762712, Blast_Score=206, Evalue=4e-53,
Organism=Drosophila melanogaster, GI24646914, Length=260, Percent_Identity=37.6923076923077, Blast_Score=164, Evalue=1e-40,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51977; Mature: 51846

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEERASCWRRRAKIVATIGPASWSPPVLDQLIAAGVDVFRVNAAHNSPEERRPIVADLR
CCCHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHCCCHHHHHH
AAADRAGRPIAILQDLASWKPRTGPLEGDSFRLIRDTRVRIVPGSEPLSPERLSIDDAEL
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCHHHH
VAQLRPGHRILISDGLIELVVESRAGDELIARVLRGGQIRGRQGVAVPGVLGRPFRLSER
HHHCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
DRADIAFAAEHELEYIGVSFVTGPDDLRSVRAILRECGGRARLVAKIERPEALAAIREIA
CCCCEEEEECCCEEEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
RESDAVMVARGDLGVQLPPEEVPIAQKRIIRVARAFGIPSIIATQMLESMTTQPIPTRAE
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHH
VSDVANAVLEGADALMLSAETATGQYPVEAVEMMHRIIVATEQQLTPAPVPEEEEPTTIA
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
ATIARTATDLARRWRAVRTIVAITRSGFTAREVARERPVAPIVAVTCDPMVARQLSLVWG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
IVPLALPSFAETSEELIEQAAGAARASGLVRGGDHAVFITSLRYFPEPGHADTLHLRQL
HHHHHCCCHHHHHHHHHHHHHCHHHHCCCEECCCCEEEEEEECCCCCCCCCCCEEECCC
>Mature Secondary Structure 
AEERASCWRRRAKIVATIGPASWSPPVLDQLIAAGVDVFRVNAAHNSPEERRPIVADLR
CCHHHHHHHHHCEEEEEECCCCCCCHHHHHHHHCCCCEEEEECCCCCCHHCCCHHHHHH
AAADRAGRPIAILQDLASWKPRTGPLEGDSFRLIRDTRVRIVPGSEPLSPERLSIDDAEL
HHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEEECCEEEEECCCCCCCCCCCCCCHHHH
VAQLRPGHRILISDGLIELVVESRAGDELIARVLRGGQIRGRQGVAVPGVLGRPFRLSER
HHHCCCCCEEEEECCHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
DRADIAFAAEHELEYIGVSFVTGPDDLRSVRAILRECGGRARLVAKIERPEALAAIREIA
CCCCEEEEECCCEEEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHH
RESDAVMVARGDLGVQLPPEEVPIAQKRIIRVARAFGIPSIIATQMLESMTTQPIPTRAE
CCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHH
VSDVANAVLEGADALMLSAETATGQYPVEAVEMMHRIIVATEQQLTPAPVPEEEEPTTIA
HHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHH
ATIARTATDLARRWRAVRTIVAITRSGFTAREVARERPVAPIVAVTCDPMVARQLSLVWG
HHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHH
IVPLALPSFAETSEELIEQAAGAARASGLVRGGDHAVFITSLRYFPEPGHADTLHLRQL
HHHHHCCCHHHHHHHHHHHHHCHHHHCCCEECCCCEEEEEEECCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]