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The map label for this gene is sigA [H]

Identifier: 221633686

GI number: 221633686

Start: 1716317

End: 1717846

Strand: Reverse

Name: sigA [H]

Synonym: trd_1713

Alternate gene names: 221633686

Gene position: 1717846-1716317 (Counterclockwise)

Preceding gene: 221633687

Following gene: 221633685

Centisome position: 85.76

GC content: 65.95

Gene sequence:

>1530_bases
GTGACCAAGGCGGAACGGCGCGGGAAGCCCAAGCGGTCACGTGAGGTGGTGACCAGGGCGAACGGATCGGTCGATCTCCC
GGTGCTCGCAGGGGCTGATGCGATCCTGGCGACGGCTCGCCTGCACGGCCGTCTCACGCGTGAGCAATGGTTGGCCCTGA
TCGACACGCTCGGCCCCGATGCTGCCGAGACGGCTCTCGCTGAGCTGCAAGCAGAGGGAGTCCCCGTCGCGATCGAGGAA
GCGCTCGAACCGGATGGCACTTCCCGCTCCTGGGAGCCTGAGAAGGAGGCCGATCCGGATCTGCTCGTCGAGGATCCGGT
GCGCTTGTACCTCGCGGAGATCGGGCGTGTCCCGCTCTTGACGGCCGAGCAGGAAGTGGAGCTCGGCCGAGCAGTGGAGT
TGGCGGAGTATCTCGACGAACTTCGGCGCTGCTGCGGTGACGATCCGCTCGTTTTGCTGCTGGCGGCGTGGGAGCGCTTC
GTGACGGGTTGGCCGCTCGTCGAGCGCCTGGCCCAGCGTCTTCCAGGCAAGAGGGAGACACGCACCGCGGTGCTCGCTGC
GATCTTGCCGCTGCGCTCCCTTCCGCCCGTGCTGCTGCGACAGTTCCGTGGGAAGGATGGCAGGAGCGTCGAGGAACTCG
AGGACACCTTGCGGTGCCGGCGCCTGGAGTTCGCGCTCTTTCCCGAGCCTTTGCAACGGTGGTGTGATCCGCTCGAGCGC
AGGCTTCCCCCGATTCCGCCGCTGAACGAGCTCGGACTGGATCGAACAGCGCTGATCGCGCACTGGCAGCGCCTCCGGCG
CGACGGGGAGGCGGCTCGTCGCAAGCTGACCGAGGCAAATCTCCGGCTCGTCGTCAGCATCGCCAAGCGGTATACCGGCC
GGGGATTGACGCTCCTGGATCTGATCCAGGAGGGGAATCTCGGGCTGATGCGGGCGGTCGACAAGTTCCAGACGCACAAG
GGCTTCAAGTTCTCGACCTATGCGACCTGGTGGATCCGCCAAGCGATCACGCGTGCCTTGGCTGATCATGCCCGGACGAT
CCGCTTGCCGGTCCACCTCGTCGAGACCTTGAACCGCGTCAGCCGTGTGGCACGCCAATTGACGCAGGAACTCGGGCGCG
AGCCGACGACCAGCGAACTCGCGGCCGCTGTCAACATGCCGCTGGATCGCCTACGCGAATTGCTCCAGGCGGCACAGGAT
CCGGTTTCGCTGGAAGCGCCGGTGGGCCAGGAAGAGGAGAGCACACTGGGTGACTTTCTGGAAGACGAGCGCGCGGTTGC
CCCGTTGGATGCAGCAGCGCTTTCGCTCTTGCGCGAGCAGGTGCAGTCGCTCCTGGCGACGTTGACTGAGCGTGAGCGCC
GAGTCCTCGCCATGCGCTTCGGTCTCGAAGATGGGCAGACCTACACGTTGGAAGAAGTCGGTCGAGCGTTCGGTGTCACG
CGCGAGCGAGTCCGCCAGATCGAGGCGAAGGCGCTTCGCAAACTCCGTCATCCGCAGCGGGCGCGAGCACTCCGCGATTT
CCTCGAGTAA

Upstream 100 bases:

>100_bases
AGGGGCGCGATACCGTTGAATTTTGCTCTGCTCCGTAGTATTACTGGGATGCCGCACGAGCAGGCGCTGGTTTCCTCGAA
GGCCGTGAGGAGTGCGCGCG

Downstream 100 bases:

>100_bases
GCTTGATCGTGTGTCAACGGAACGTTGCGAGCAGGTCTGCCGAGCGGCAGGCCGGAGGGGTCATGTTCTACGACGAAGCC
AAGATCTTCGTGAAGGCTGG

Product: RNA polymerase sigma 70 subunit, RpoD

Products: NA

Alternate protein names: Sigma-A [H]

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MTKAERRGKPKRSREVVTRANGSVDLPVLAGADAILATARLHGRLTREQWLALIDTLGPDAAETALAELQAEGVPVAIEE
ALEPDGTSRSWEPEKEADPDLLVEDPVRLYLAEIGRVPLLTAEQEVELGRAVELAEYLDELRRCCGDDPLVLLLAAWERF
VTGWPLVERLAQRLPGKRETRTAVLAAILPLRSLPPVLLRQFRGKDGRSVEELEDTLRCRRLEFALFPEPLQRWCDPLER
RLPPIPPLNELGLDRTALIAHWQRLRRDGEAARRKLTEANLRLVVSIAKRYTGRGLTLLDLIQEGNLGLMRAVDKFQTHK
GFKFSTYATWWIRQAITRALADHARTIRLPVHLVETLNRVSRVARQLTQELGREPTTSELAAAVNMPLDRLRELLQAAQD
PVSLEAPVGQEEESTLGDFLEDERAVAPLDAAALSLLREQVQSLLATLTERERRVLAMRFGLEDGQTYTLEEVGRAFGVT
RERVRQIEAKALRKLRHPQRARALRDFLE

Sequences:

>Translated_509_residues
MTKAERRGKPKRSREVVTRANGSVDLPVLAGADAILATARLHGRLTREQWLALIDTLGPDAAETALAELQAEGVPVAIEE
ALEPDGTSRSWEPEKEADPDLLVEDPVRLYLAEIGRVPLLTAEQEVELGRAVELAEYLDELRRCCGDDPLVLLLAAWERF
VTGWPLVERLAQRLPGKRETRTAVLAAILPLRSLPPVLLRQFRGKDGRSVEELEDTLRCRRLEFALFPEPLQRWCDPLER
RLPPIPPLNELGLDRTALIAHWQRLRRDGEAARRKLTEANLRLVVSIAKRYTGRGLTLLDLIQEGNLGLMRAVDKFQTHK
GFKFSTYATWWIRQAITRALADHARTIRLPVHLVETLNRVSRVARQLTQELGREPTTSELAAAVNMPLDRLRELLQAAQD
PVSLEAPVGQEEESTLGDFLEDERAVAPLDAAALSLLREQVQSLLATLTERERRVLAMRFGLEDGQTYTLEEVGRAFGVT
RERVRQIEAKALRKLRHPQRARALRDFLE
>Mature_508_residues
TKAERRGKPKRSREVVTRANGSVDLPVLAGADAILATARLHGRLTREQWLALIDTLGPDAAETALAELQAEGVPVAIEEA
LEPDGTSRSWEPEKEADPDLLVEDPVRLYLAEIGRVPLLTAEQEVELGRAVELAEYLDELRRCCGDDPLVLLLAAWERFV
TGWPLVERLAQRLPGKRETRTAVLAAILPLRSLPPVLLRQFRGKDGRSVEELEDTLRCRRLEFALFPEPLQRWCDPLERR
LPPIPPLNELGLDRTALIAHWQRLRRDGEAARRKLTEANLRLVVSIAKRYTGRGLTLLDLIQEGNLGLMRAVDKFQTHKG
FKFSTYATWWIRQAITRALADHARTIRLPVHLVETLNRVSRVARQLTQELGREPTTSELAAAVNMPLDRLRELLQAAQDP
VSLEAPVGQEEESTLGDFLEDERAVAPLDAAALSLLREQVQSLLATLTERERRVLAMRFGLEDGQTYTLEEVGRAFGVTR
ERVRQIEAKALRKLRHPQRARALRDFLE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=245, Percent_Identity=60.4081632653061, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1789098, Length=234, Percent_Identity=47.8632478632479, Blast_Score=222, Evalue=4e-59,
Organism=Escherichia coli, GI1789871, Length=248, Percent_Identity=31.8548387096774, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1788231, Length=202, Percent_Identity=30.6930693069307, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001909
- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 57473; Mature: 57342

Theoretical pI: Translated: 6.81; Mature: 6.81

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKAERRGKPKRSREVVTRANGSVDLPVLAGADAILATARLHGRLTREQWLALIDTLGPD
CCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AAETALAELQAEGVPVAIEEALEPDGTSRSWEPEKEADPDLLVEDPVRLYLAEIGRVPLL
HHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEE
TAEQEVELGRAVELAEYLDELRRCCGDDPLVLLLAAWERFVTGWPLVERLAQRLPGKRET
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
RTAVLAAILPLRSLPPVLLRQFRGKDGRSVEELEDTLRCRRLEFALFPEPLQRWCDPLER
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RLPPIPPLNELGLDRTALIAHWQRLRRDGEAARRKLTEANLRLVVSIAKRYTGRGLTLLD
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LIQEGNLGLMRAVDKFQTHKGFKFSTYATWWIRQAITRALADHARTIRLPVHLVETLNRV
HHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
SRVARQLTQELGREPTTSELAAAVNMPLDRLRELLQAAQDPVSLEAPVGQEEESTLGDFL
HHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHH
EDERAVAPLDAAALSLLREQVQSLLATLTERERRVLAMRFGLEDGQTYTLEEVGRAFGVT
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCHH
RERVRQIEAKALRKLRHPQRARALRDFLE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHC
>Mature Secondary Structure 
TKAERRGKPKRSREVVTRANGSVDLPVLAGADAILATARLHGRLTREQWLALIDTLGPD
CCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
AAETALAELQAEGVPVAIEEALEPDGTSRSWEPEKEADPDLLVEDPVRLYLAEIGRVPLL
HHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEE
TAEQEVELGRAVELAEYLDELRRCCGDDPLVLLLAAWERFVTGWPLVERLAQRLPGKRET
CCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHH
RTAVLAAILPLRSLPPVLLRQFRGKDGRSVEELEDTLRCRRLEFALFPEPLQRWCDPLER
HHHHHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RLPPIPPLNELGLDRTALIAHWQRLRRDGEAARRKLTEANLRLVVSIAKRYTGRGLTLLD
CCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LIQEGNLGLMRAVDKFQTHKGFKFSTYATWWIRQAITRALADHARTIRLPVHLVETLNRV
HHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHH
SRVARQLTQELGREPTTSELAAAVNMPLDRLRELLQAAQDPVSLEAPVGQEEESTLGDFL
HHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCEECCCCCCCHHHHHHHHH
EDERAVAPLDAAALSLLREQVQSLLATLTERERRVLAMRFGLEDGQTYTLEEVGRAFGVT
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHCHH
RERVRQIEAKALRKLRHPQRARALRDFLE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA