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The map label for this gene is rfbD [H]

Identifier: 221633639

GI number: 221633639

Start: 1662814

End: 1663659

Strand: Reverse

Name: rfbD [H]

Synonym: trd_1664

Alternate gene names: 221633639

Gene position: 1663659-1662814 (Counterclockwise)

Preceding gene: 221633643

Following gene: 221633637

Centisome position: 83.06

GC content: 68.2

Gene sequence:

>846_bases
GTGGTGCGCGTGCTCATCACCGGTGGACACGGCCAACTGGGACGTGCGCTGGTGCGCACCGCTCCAGCGCACACGGAGGT
GATCGCGCTGGGAGCGGCCGAGTGCGACGTGACCGATCCCTTCACCGTGCGTGCGGTGCTCGCTCGGGTCGAACCGACGC
TCGTCATCCACTGTGCAGCCTGGACCGATGTCGACGGCTGCGAGCGGCATCCGGAACGGGCCTGGCGCGTCAATGCGCTG
GGCACGCAGCACATCGCGGCTGCCTGCGCGGCCGCCGAGGCAGCGCTGGTCGCGCTTTCCACCAATTATGTCTTCGATGG
AGAGCAGGACGAGCCGTATCACGAGTTCGCGCGACCGCATCCGTTGAGCGTGTACGGGGCAAGCAAGCTGGCAGCCGAGG
AGGTCGTCCGCGCACTCTGCCCGCGCCATTACATCGTGCGGACGGCCATGCTTTACGACGCGCACGGGCGCAACTTCGTC
ACGACCGTTCTGCGACTCGCGCGGGAGCAACCACGCTTGCGGATGGTCGCCGATCAATACGGGAGCCCCACCTTCGTGGA
CGATCTAGCGAAAGCGATCTGGCAACTGGTCGCTCAACCGGCCTACGGGACGTACCATCTCGTCAACGCCGGCAGGGCTT
CCTGGTACGAGTGGGCGGTCGCGGTGCTGGAGACGCTCGGACTCGGCGTGCCAGTCGAGCCGATTCCGGCGAGCCAGTTC
CGGCGGGCGGCTCGCCCACCGCGCAACGGAGTCTTGACCAGCCTGGCAGCGCCGGCGCTGGGTATCGTCCTCCCCGATTG
GCGCGACGCGCTCACGCGCTGCTTGGCCCCGCTCCGCTCTGCCTGA

Upstream 100 bases:

>100_bases
GCGCTCCGAACCGCACGTATCGCTCCTTCCACTTCGTCCTCGAACGCGCTCCCAGGCCCCACGCGCACGCTATAGTAACC
GACGACGTCTGCGGAGGTCG

Downstream 100 bases:

>100_bases
GCGATGGAGCGGTCAGCGATGCAACGATGCGGTGACGAGTGCCGAGTCGGCGGAGCGTCCATGCCAGTGAGACCACGTTG
AGCGCGAGCACGATCCCGGT

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MVRVLITGGHGQLGRALVRTAPAHTEVIALGAAECDVTDPFTVRAVLARVEPTLVIHCAAWTDVDGCERHPERAWRVNAL
GTQHIAAACAAAEAALVALSTNYVFDGEQDEPYHEFARPHPLSVYGASKLAAEEVVRALCPRHYIVRTAMLYDAHGRNFV
TTVLRLAREQPRLRMVADQYGSPTFVDDLAKAIWQLVAQPAYGTYHLVNAGRASWYEWAVAVLETLGLGVPVEPIPASQF
RRAARPPRNGVLTSLAAPALGIVLPDWRDALTRCLAPLRSA

Sequences:

>Translated_281_residues
MVRVLITGGHGQLGRALVRTAPAHTEVIALGAAECDVTDPFTVRAVLARVEPTLVIHCAAWTDVDGCERHPERAWRVNAL
GTQHIAAACAAAEAALVALSTNYVFDGEQDEPYHEFARPHPLSVYGASKLAAEEVVRALCPRHYIVRTAMLYDAHGRNFV
TTVLRLAREQPRLRMVADQYGSPTFVDDLAKAIWQLVAQPAYGTYHLVNAGRASWYEWAVAVLETLGLGVPVEPIPASQF
RRAARPPRNGVLTSLAAPALGIVLPDWRDALTRCLAPLRSA
>Mature_281_residues
MVRVLITGGHGQLGRALVRTAPAHTEVIALGAAECDVTDPFTVRAVLARVEPTLVIHCAAWTDVDGCERHPERAWRVNAL
GTQHIAAACAAAEAALVALSTNYVFDGEQDEPYHEFARPHPLSVYGASKLAAEEVVRALCPRHYIVRTAMLYDAHGRNFV
TTVLRLAREQPRLRMVADQYGSPTFVDDLAKAIWQLVAQPAYGTYHLVNAGRASWYEWAVAVLETLGLGVPVEPIPASQF
RRAARPPRNGVLTSLAAPALGIVLPDWRDALTRCLAPLRSA

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=278, Percent_Identity=28.7769784172662, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI11034825, Length=278, Percent_Identity=28.7769784172662, Blast_Score=89, Evalue=3e-18,
Organism=Escherichia coli, GI1788352, Length=293, Percent_Identity=34.1296928327645, Blast_Score=155, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 30578; Mature: 30578

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRVLITGGHGQLGRALVRTAPAHTEVIALGAAECDVTDPFTVRAVLARVEPTLVIHCAA
CEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEEC
WTDVDGCERHPERAWRVNALGTQHIAAACAAAEAALVALSTNYVFDGEQDEPYHEFARPH
CCCCCHHHCCCHHHEEECCCCHHHHHHHHHHHHHHEEEEECCEEECCCCCCCHHHHCCCC
PLSVYGASKLAAEEVVRALCPRHYIVRTAMLYDAHGRNFVTTVLRLAREQPRLRMVADQY
CEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHC
GSPTFVDDLAKAIWQLVAQPAYGTYHLVNAGRASWYEWAVAVLETLGLGVPVEPIPASQF
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RRAARPPRNGVLTSLAAPALGIVLPDWRDALTRCLAPLRSA
HHHCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVRVLITGGHGQLGRALVRTAPAHTEVIALGAAECDVTDPFTVRAVLARVEPTLVIHCAA
CEEEEEECCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCEEEEEEEC
WTDVDGCERHPERAWRVNALGTQHIAAACAAAEAALVALSTNYVFDGEQDEPYHEFARPH
CCCCCHHHCCCHHHEEECCCCHHHHHHHHHHHHHHEEEEECCEEECCCCCCCHHHHCCCC
PLSVYGASKLAAEEVVRALCPRHYIVRTAMLYDAHGRNFVTTVLRLAREQPRLRMVADQY
CEEECCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHC
GSPTFVDDLAKAIWQLVAQPAYGTYHLVNAGRASWYEWAVAVLETLGLGVPVEPIPASQF
CCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHH
RRAARPPRNGVLTSLAAPALGIVLPDWRDALTRCLAPLRSA
HHHCCCCCCCHHHHHHHHHHEEECCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]