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The map label for this gene is cpg2 [H]

Identifier: 221633437

GI number: 221633437

Start: 1447073

End: 1448209

Strand: Reverse

Name: cpg2 [H]

Synonym: trd_1459

Alternate gene names: 221633437

Gene position: 1448209-1447073 (Counterclockwise)

Preceding gene: 221633438

Following gene: 221633436

Centisome position: 72.3

GC content: 66.4

Gene sequence:

>1137_bases
ATGACGATCTCGATCGCTGACGTCCTGCCCAGGCGCGAGGCGCTCCTCACCGAACTCCGCACTCTGGTCGAGCTGGAGTC
CCCCAGCACCGACAAGATAGCGGTCGATCGTCTCGTCGCGTACGTTCGCTCGCGAGCACGTCAACTCGGAGCGTCGATCG
AGGAATACCCACAGCAGGAGTACGGTGATCTCACGATCGCCAGTTGGCCAGCCCAGGGGAATGCGTCAGCTGAGCCATTG
CTCGTGCTCACCCATCTCGACACCGTCTGGCCGGTCGGGACGGTGGAGCACCGTCCCTTCCGGATCGAAGGAGACCTCGC
CTTCGGCCCGGGGATCTACGACATGAAGGCGAGCATCGCCATGTTCCTGGAGGCGATGCGCTTTCTCCAGGAGCGGCGGC
TGGCGCACCGGCCGATCCGCTGGTTGATCAATACCGAGGAGGAAGTCGGCAGTCCTGTCTCGCGACCGCTGATCGAGGAC
GAAGCCCGGCGTTGTGCGGTCGTTTTCTGCCTGGAACCGCCAGCACCGGGCGGGGCGCTCAAGACGGCGCGGAAGGGTGT
CGGGATCTTCACGGTGCGGATCACTGGGCGTGCTGCTCATGCCGGTGCTGACCCCGAGCGGGGGGTGAGCGCGATCCAGG
AGTTGGCCAACCAGATCGCCTACCTCCACAGCTTGAGCGATCCGACGCGCGGGACGACCGTCAACGTCGGAGTCGTCGGT
GGTGGAACGCGTTCCAACGTGATTGCTGCCGAGGCGTGGGCACAGGTCGACCTCCGCGTGGTGACACCCGAGGAAGCCGA
GCGGGTGACCGCGGCTATCCTGGGAACGCGCCCCTGGCTGCGGGGAGCGACTGTGCAGGTGCACGGTGGGATGAATCGCC
CGCCGATGGAGCGGACACCGGCGGTCGCTCGAGCGTTCGAGCGGGCACGCGCGATCGCACGAGAACTGGGGCTGGAGATC
GGTGAAGCAGCGACCGGTGGGGCGAGCGATGGCAACTTCACGGCTGCACTGGGTCTGCCGACGCTGGATGGGCTGGGTTG
CCCCGGTGACGGTGCCCACGCCGAGCACGAGCAGGTCAGCATTGCGGGGCTGATCGAACGGACGGCTTTCCTGACGGCGC
TCTTGAACCGCTGGTAA

Upstream 100 bases:

>100_bases
CTGCGCCATTTCAGTCAGCCGCGCCCACCGGCCGCGGGCCGCGTGCGCCCCGAACCGGAGCATGGCGAAGAAGAGGTCTG
GCAGGAGCAGGAGCTGGCTG

Downstream 100 bases:

>100_bases
GGAAGGCTGGGATGGATAGGGACGAGAAGGAAAGGGGTTGACTGATGCGACTCGAAGTCGATCCGCTGCAGATTTTGCCG
CGTGTCCTGACGCGCTACTC

Product: putative carboxypeptidase G2

Products: NA

Alternate protein names: CPDG2; Folate hydrolase G2; Glutamate carboxypeptidase; Pteroylmonoglutamic acid hydrolase G2; Glucarpidase [H]

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTISIADVLPRREALLTELRTLVELESPSTDKIAVDRLVAYVRSRARQLGASIEEYPQQEYGDLTIASWPAQGNASAEPL
LVLTHLDTVWPVGTVEHRPFRIEGDLAFGPGIYDMKASIAMFLEAMRFLQERRLAHRPIRWLINTEEEVGSPVSRPLIED
EARRCAVVFCLEPPAPGGALKTARKGVGIFTVRITGRAAHAGADPERGVSAIQELANQIAYLHSLSDPTRGTTVNVGVVG
GGTRSNVIAAEAWAQVDLRVVTPEEAERVTAAILGTRPWLRGATVQVHGGMNRPPMERTPAVARAFERARAIARELGLEI
GEAATGGASDGNFTAALGLPTLDGLGCPGDGAHAEHEQVSIAGLIERTAFLTALLNRW

Sequences:

>Translated_378_residues
MTISIADVLPRREALLTELRTLVELESPSTDKIAVDRLVAYVRSRARQLGASIEEYPQQEYGDLTIASWPAQGNASAEPL
LVLTHLDTVWPVGTVEHRPFRIEGDLAFGPGIYDMKASIAMFLEAMRFLQERRLAHRPIRWLINTEEEVGSPVSRPLIED
EARRCAVVFCLEPPAPGGALKTARKGVGIFTVRITGRAAHAGADPERGVSAIQELANQIAYLHSLSDPTRGTTVNVGVVG
GGTRSNVIAAEAWAQVDLRVVTPEEAERVTAAILGTRPWLRGATVQVHGGMNRPPMERTPAVARAFERARAIARELGLEI
GEAATGGASDGNFTAALGLPTLDGLGCPGDGAHAEHEQVSIAGLIERTAFLTALLNRW
>Mature_377_residues
TISIADVLPRREALLTELRTLVELESPSTDKIAVDRLVAYVRSRARQLGASIEEYPQQEYGDLTIASWPAQGNASAEPLL
VLTHLDTVWPVGTVEHRPFRIEGDLAFGPGIYDMKASIAMFLEAMRFLQERRLAHRPIRWLINTEEEVGSPVSRPLIEDE
ARRCAVVFCLEPPAPGGALKTARKGVGIFTVRITGRAAHAGADPERGVSAIQELANQIAYLHSLSDPTRGTTVNVGVVGG
GTRSNVIAAEAWAQVDLRVVTPEEAERVTAAILGTRPWLRGATVQVHGGMNRPPMERTPAVARAFERARAIARELGLEIG
EAATGGASDGNFTAALGLPTLDGLGCPGDGAHAEHEQVSIAGLIERTAFLTALLNRW

Specific function: Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity [H]

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family [H]

Homologues:

Organism=Escherichia coli, GI1790395, Length=264, Percent_Identity=28.4090909090909, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001261
- InterPro:   IPR017150
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: =3.4.17.11 [H]

Molecular weight: Translated: 40702; Mature: 40571

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00318 HMG_COA_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTISIADVLPRREALLTELRTLVELESPSTDKIAVDRLVAYVRSRARQLGASIEEYPQQE
CEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHH
YGDLTIASWPAQGNASAEPLLVLTHLDTVWPVGTVEHRPFRIEGDLAFGPGIYDMKASIA
HCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCEEECCCHHHHHHHHH
MFLEAMRFLQERRLAHRPIRWLINTEEEVGSPVSRPLIEDEARRCAVVFCLEPPAPGGAL
HHHHHHHHHHHHHHHHCCHHEEECCHHHHCCCHHCCHHHHCCCEEEEEEEECCCCCCCHH
KTARKGVGIFTVRITGRAAHAGADPERGVSAIQELANQIAYLHSLSDPTRGTTVNVGVVG
HHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
GGTRSNVIAAEAWAQVDLRVVTPEEAERVTAAILGTRPWLRGATVQVHGGMNRPPMERTP
CCCCCCEEEEEHHEEEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCH
AVARAFERARAIARELGLEIGEAATGGASDGNFTAALGLPTLDGLGCPGDGAHAEHEQVS
HHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHH
IAGLIERTAFLTALLNRW
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TISIADVLPRREALLTELRTLVELESPSTDKIAVDRLVAYVRSRARQLGASIEEYPQQE
EEEHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHH
YGDLTIASWPAQGNASAEPLLVLTHLDTVWPVGTVEHRPFRIEGDLAFGPGIYDMKASIA
HCCEEEEECCCCCCCCCCCEEEEEECCCCCCCCCCCCCCEEEECCEEECCCHHHHHHHHH
MFLEAMRFLQERRLAHRPIRWLINTEEEVGSPVSRPLIEDEARRCAVVFCLEPPAPGGAL
HHHHHHHHHHHHHHHHCCHHEEECCHHHHCCCHHCCHHHHCCCEEEEEEEECCCCCCCHH
KTARKGVGIFTVRITGRAAHAGADPERGVSAIQELANQIAYLHSLSDPTRGTTVNVGVVG
HHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
GGTRSNVIAAEAWAQVDLRVVTPEEAERVTAAILGTRPWLRGATVQVHGGMNRPPMERTP
CCCCCCEEEEEHHEEEEEEEECCHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCH
AVARAFERARAIARELGLEIGEAATGGASDGNFTAALGLPTLDGLGCPGDGAHAEHEQVS
HHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHH
IAGLIERTAFLTALLNRW
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6396165; 3838935; 9083113 [H]