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The map label for this gene is gabT [C]

Identifier: 221633436

GI number: 221633436

Start: 1445751

End: 1447028

Strand: Reverse

Name: gabT [C]

Synonym: trd_1458

Alternate gene names: 221633436

Gene position: 1447028-1445751 (Counterclockwise)

Preceding gene: 221633437

Following gene: 221633435

Centisome position: 72.24

GC content: 64.79

Gene sequence:

>1278_bases
ATGCGACTCGAAGTCGATCCGCTGCAGATTTTGCCGCGTGTCCTGACGCGCTACTCATCGGTCGTCGTCGACCGGGCCGA
AGGGATCTACGTCTGGGATATCGAGGGCCAGCGTTGGATGGACTTCACCTCGGGGATCGCGGTCGTCAACACCGGGCACT
GCCACCCGCGCGTGGTGGCGGCGATCCGCGAACAGGCGGGCAAGATCATCCATGCCCAGGCCAACATCCTGATCCACGAG
CCGATGCTGCGGCTCTCCCAACTCATCACCGCGACGATGCCACCCACGCTGAACCAGGTCTTCTTCACCAACTCAGGGGC
TGAAGCGGTAGAGGGCGCGGTCAAGCTGGCCAAGGCGGCGACCGGACGCCCGGCCATCATCGCGTTCCGCGGGGCCTTCC
ACGGTCGGACGCACCTGGCGATGGCCTTGACGACCTCGCGGGTCAAGGTGCGCGGCCATTACGAGCCGCTGGTTCCCTCG
ATCTACTATGCTCCGTATCCGTACCCGTTCCGGAACCCGTACAACGTCCCAGCGGAGGATGTCGACCTCGTCTGCATCGC
GGAGCTGGAGCGCCTGTTCCAGACCATGGTGATGCCGGACGACGTCGCGGCGATCATCGTGGAGCCAATGCTCGGCGAGG
GTGGCTACGTCCTTCCTCCCAAGCGGTTCCTCCAGAATCTGCGGGCCATCTGCGACCGTTACGGCATCCTGCTCATCGTC
GACGAGATCCAGACGGGGGTCGGTCGGACGGGGAAGATGTGGGCGTTCGAGCACTTCGAGATTGTGCCGGACATCGTCAC
GGTTGCCAAGGGGATCGCTTCCGGACTCCCCTTGGCCGCGGTGGTGGCCAACAAGGAACTGATGGATCGCTGGGAGCCGG
GTGCGCATGGCGGTACCTATGGTGGCAATGCCGTCGCCTGTGCGGCCGGTGTGGCGACCTTCGAAGTGATGCGCGAGGAG
AAGCTGCCGGAAAACGCAGCACGCATCGGCGGGTATCTCATGGCGCAGCTCCGCGAGCTGCAGCAGGAGTTCCCGGTGAT
CGGTGAGGTGCGCGGGCTCGGCCTGATGATCGGCGTCGAGCTGATCAAGCCCGATCGGGCTCCCAATCCGGAGGCAGTCA
AGCGGGTGGTCAAGCGGGCACAGGAACTCCGAACGCTCCTCATCACGGCTGGCGAGCACGATCAGGTGATCCGCGTCATC
CCACCGCTCGTCATCACGCAGGAGCAGGCCGATGCATTCCTGGACGTCTTCAGCGAGGCGCTGAAGGCGGCCGAGTGA

Upstream 100 bases:

>100_bases
TTGCGGGGCTGATCGAACGGACGGCTTTCCTGACGGCGCTCTTGAACCGCTGGTAAGGAAGGCTGGGATGGATAGGGACG
AGAAGGAAAGGGGTTGACTG

Downstream 100 bases:

>100_bases
CGGGGTCGCGAATCACCGAAGTGGTGGAGCGCTCGCAGCATTGGCGGGAAGTCAGCGCTGCGAGCGCTTTTCCTGCCCGA
GCGCTGCTGGCGAGCCGGTA

Product: putative 4-aminobutyrate aminotransferase

Products: succinate semialdehyde; L-glutamate

Alternate protein names: ORF B [H]

Number of amino acids: Translated: 425; Mature: 425

Protein sequence:

>425_residues
MRLEVDPLQILPRVLTRYSSVVVDRAEGIYVWDIEGQRWMDFTSGIAVVNTGHCHPRVVAAIREQAGKIIHAQANILIHE
PMLRLSQLITATMPPTLNQVFFTNSGAEAVEGAVKLAKAATGRPAIIAFRGAFHGRTHLAMALTTSRVKVRGHYEPLVPS
IYYAPYPYPFRNPYNVPAEDVDLVCIAELERLFQTMVMPDDVAAIIVEPMLGEGGYVLPPKRFLQNLRAICDRYGILLIV
DEIQTGVGRTGKMWAFEHFEIVPDIVTVAKGIASGLPLAAVVANKELMDRWEPGAHGGTYGGNAVACAAGVATFEVMREE
KLPENAARIGGYLMAQLRELQQEFPVIGEVRGLGLMIGVELIKPDRAPNPEAVKRVVKRAQELRTLLITAGEHDQVIRVI
PPLVITQEQADAFLDVFSEALKAAE

Sequences:

>Translated_425_residues
MRLEVDPLQILPRVLTRYSSVVVDRAEGIYVWDIEGQRWMDFTSGIAVVNTGHCHPRVVAAIREQAGKIIHAQANILIHE
PMLRLSQLITATMPPTLNQVFFTNSGAEAVEGAVKLAKAATGRPAIIAFRGAFHGRTHLAMALTTSRVKVRGHYEPLVPS
IYYAPYPYPFRNPYNVPAEDVDLVCIAELERLFQTMVMPDDVAAIIVEPMLGEGGYVLPPKRFLQNLRAICDRYGILLIV
DEIQTGVGRTGKMWAFEHFEIVPDIVTVAKGIASGLPLAAVVANKELMDRWEPGAHGGTYGGNAVACAAGVATFEVMREE
KLPENAARIGGYLMAQLRELQQEFPVIGEVRGLGLMIGVELIKPDRAPNPEAVKRVVKRAQELRTLLITAGEHDQVIRVI
PPLVITQEQADAFLDVFSEALKAAE
>Mature_425_residues
MRLEVDPLQILPRVLTRYSSVVVDRAEGIYVWDIEGQRWMDFTSGIAVVNTGHCHPRVVAAIREQAGKIIHAQANILIHE
PMLRLSQLITATMPPTLNQVFFTNSGAEAVEGAVKLAKAATGRPAIIAFRGAFHGRTHLAMALTTSRVKVRGHYEPLVPS
IYYAPYPYPFRNPYNVPAEDVDLVCIAELERLFQTMVMPDDVAAIIVEPMLGEGGYVLPPKRFLQNLRAICDRYGILLIV
DEIQTGVGRTGKMWAFEHFEIVPDIVTVAKGIASGLPLAAVVANKELMDRWEPGAHGGTYGGNAVACAAGVATFEVMREE
KLPENAARIGGYLMAQLRELQQEFPVIGEVRGLGLMIGVELIKPDRAPNPEAVKRVVKRAQELRTLLITAGEHDQVIRVI
PPLVITQEQADAFLDVFSEALKAAE

Specific function: 4-aminobutyrate (GABA) degradation. [C]

COG id: COG0160

COG function: function code E; 4-aminobutyrate aminotransferase and related aminotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Homo sapiens, GI13994255, Length=442, Percent_Identity=31.9004524886878, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI37574042, Length=409, Percent_Identity=32.0293398533007, Blast_Score=228, Evalue=6e-60,
Organism=Homo sapiens, GI226442709, Length=382, Percent_Identity=32.1989528795811, Blast_Score=214, Evalue=8e-56,
Organism=Homo sapiens, GI226442705, Length=409, Percent_Identity=30.5623471882641, Blast_Score=214, Evalue=2e-55,
Organism=Homo sapiens, GI24119277, Length=427, Percent_Identity=32.7868852459016, Blast_Score=213, Evalue=2e-55,
Organism=Homo sapiens, GI4557809, Length=410, Percent_Identity=32.6829268292683, Blast_Score=209, Evalue=5e-54,
Organism=Homo sapiens, GI284507298, Length=327, Percent_Identity=33.9449541284404, Blast_Score=155, Evalue=8e-38,
Organism=Homo sapiens, GI188536080, Length=443, Percent_Identity=27.0880361173815, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI38679950, Length=443, Percent_Identity=27.0880361173815, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI38679946, Length=443, Percent_Identity=27.0880361173815, Blast_Score=133, Evalue=3e-31,
Organism=Escherichia coli, GI1789016, Length=421, Percent_Identity=40.1425178147268, Blast_Score=323, Evalue=1e-89,
Organism=Escherichia coli, GI1787560, Length=418, Percent_Identity=39.4736842105263, Blast_Score=298, Evalue=4e-82,
Organism=Escherichia coli, GI1788044, Length=405, Percent_Identity=35.0617283950617, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI145693181, Length=401, Percent_Identity=35.4114713216958, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1789759, Length=404, Percent_Identity=34.6534653465347, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI1786349, Length=345, Percent_Identity=30.4347826086957, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1786991, Length=397, Percent_Identity=30.2267002518892, Blast_Score=145, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI32564660, Length=440, Percent_Identity=35, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI25144271, Length=408, Percent_Identity=32.3529411764706, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI71992977, Length=407, Percent_Identity=30.958230958231, Blast_Score=193, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI25144274, Length=294, Percent_Identity=32.6530612244898, Blast_Score=159, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17541228, Length=447, Percent_Identity=26.8456375838926, Blast_Score=119, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI71981843, Length=157, Percent_Identity=30.5732484076433, Blast_Score=79, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323470, Length=418, Percent_Identity=35.1674641148325, Blast_Score=243, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6324432, Length=435, Percent_Identity=32.6436781609195, Blast_Score=223, Evalue=7e-59,
Organism=Saccharomyces cerevisiae, GI6321456, Length=444, Percent_Identity=26.5765765765766, Blast_Score=137, Evalue=5e-33,
Organism=Saccharomyces cerevisiae, GI6324386, Length=465, Percent_Identity=25.8064516129032, Blast_Score=126, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28574759, Length=434, Percent_Identity=36.405529953917, Blast_Score=254, Evalue=7e-68,
Organism=Drosophila melanogaster, GI161085790, Length=435, Percent_Identity=36.0919540229885, Blast_Score=253, Evalue=1e-67,
Organism=Drosophila melanogaster, GI21356575, Length=428, Percent_Identity=34.1121495327103, Blast_Score=237, Evalue=1e-62,
Organism=Drosophila melanogaster, GI21357415, Length=410, Percent_Identity=32.4390243902439, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI281366494, Length=445, Percent_Identity=28.0898876404494, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24667139, Length=445, Percent_Identity=28.0898876404494, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI24667143, Length=445, Percent_Identity=28.0898876404494, Blast_Score=145, Evalue=5e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00202 Aminotran_3 [H]

EC number: 2.6.1.19

Molecular weight: Translated: 46707; Mature: 46707

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLEVDPLQILPRVLTRYSSVVVDRAEGIYVWDIEGQRWMDFTSGIAVVNTGHCHPRVVA
CCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEECCCCEEHHHHCCEEEEECCCCCHHHHH
AIREQAGKIIHAQANILIHEPMLRLSQLITATMPPTLNQVFFTNSGAEAVEGAVKLAKAA
HHHHHCCCEEEEECCEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHC
TGRPAIIAFRGAFHGRTHLAMALTTSRVKVRGHYEPLVPSIYYAPYPYPFRNPYNVPAED
CCCCEEEEEECCCCCCCEEEEEEEECEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCC
VDLVCIAELERLFQTMVMPDDVAAIIVEPMLGEGGYVLPPKRFLQNLRAICDRYGILLIV
CCCHHHHHHHHHHHHHCCCCCHHHHEEEHHCCCCCEECCHHHHHHHHHHHHHHCCEEEEE
DEIQTGVGRTGKMWAFEHFEIVPDIVTVAKGIASGLPLAAVVANKELMDRWEPGAHGGTY
ECHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHCCCCCCCCCC
GGNAVACAAGVATFEVMREEKLPENAARIGGYLMAQLRELQQEFPVIGEVRGLGLMIGVE
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEE
LIKPDRAPNPEAVKRVVKRAQELRTLLITAGEHDQVIRVIPPLVITQEQADAFLDVFSEA
EECCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCEECCCEEEEHHHHHHHHHHHHHH
LKAAE
HHCCC
>Mature Secondary Structure
MRLEVDPLQILPRVLTRYSSVVVDRAEGIYVWDIEGQRWMDFTSGIAVVNTGHCHPRVVA
CCCCCCHHHHHHHHHHHHHHHHEECCCCEEEEECCCCEEHHHHCCEEEEECCCCCHHHHH
AIREQAGKIIHAQANILIHEPMLRLSQLITATMPPTLNQVFFTNSGAEAVEGAVKLAKAA
HHHHHCCCEEEEECCEEEEHHHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHHHHC
TGRPAIIAFRGAFHGRTHLAMALTTSRVKVRGHYEPLVPSIYYAPYPYPFRNPYNVPAED
CCCCEEEEEECCCCCCCEEEEEEEECEEEEEECCCCCCCCEEECCCCCCCCCCCCCCCCC
VDLVCIAELERLFQTMVMPDDVAAIIVEPMLGEGGYVLPPKRFLQNLRAICDRYGILLIV
CCCHHHHHHHHHHHHHCCCCCHHHHEEEHHCCCCCEECCHHHHHHHHHHHHHHCCEEEEE
DEIQTGVGRTGKMWAFEHFEIVPDIVTVAKGIASGLPLAAVVANKELMDRWEPGAHGGTY
ECHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHHHHCCCCCCCCCC
GGNAVACAAGVATFEVMREEKLPENAARIGGYLMAQLRELQQEFPVIGEVRGLGLMIGVE
CCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCEEEEEEE
LIKPDRAPNPEAVKRVVKRAQELRTLLITAGEHDQVIRVIPPLVITQEQADAFLDVFSEA
EECCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCEECCCEEEEHHHHHHHHHHHHHH
LKAAE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Pyridoxal 5'-phosphate lysine-residue [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 3.3 {4-aminobutanoate}} 1.1 {4-aminobutanoate}} 1.3 {2-oxoglutarate}} 0.7 {2-oxoglutarate}} [C]

Substrates: 4-aminobutanoate; 2-oxoglutarate

Specific reaction: 4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

General reaction: Amino group transfer [C]

Inhibitor: 2-Oxoglutarate; Lysyl reagents; SH-group reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA