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The map label for this gene is dmpD [H]

Identifier: 221633370

GI number: 221633370

Start: 1380010

End: 1380855

Strand: Reverse

Name: dmpD [H]

Synonym: trd_1390

Alternate gene names: 221633370

Gene position: 1380855-1380010 (Counterclockwise)

Preceding gene: 221633371

Following gene: 221633369

Centisome position: 68.94

GC content: 65.72

Gene sequence:

>846_bases
GTGACGATCGCCAAGTCGGTCGTGCACGCGGGACGATTGCCCACCCATTTTCATCGGGCAGGAAGTGGAGACCCGGTGAT
CTTCCTCCACGGCTCTGGCCCGGGAGTGACAGCCTGGGCGAACTGGCGCTTTGCCCTTCCTGCACTGGCCAGCGACTTCG
ATTGCATCGCTCCGGATCTCTGGGGGTTTGCCCGGAGCGGGCACCCTGATCCGCCACCGATCGGCGCGCGGGCCTGGATG
GAGCTCTGGGTCGAGCAGGTCATCGCGCTCATGGAGCATCTCGGGATCGCGCGGGCGCATCTGGTCGGTAACTCGATGGG
CGGGGCGATCGCGCTGCATCTCCTCTATCGGCACCGCGACCGTTTCCAGCGTGTCGTGCTCATGGGAACGGTCGGAGCCC
CGCACCGGATGACGGAACAGCTGGACGTCGCCTGGGGTTTTTACGAGGAACCGACGGCGGAGCGCCTGGCCCAGATCATC
CGCTGGTTCGTCTACGATCCCGCGACGGTCGGTGGTGACCTGGAGGCGATCGCGCGCGACCGCCTGGCGGCGGCACTCGA
TCCAGTGGTGCGCCGGAGTTACGAGGCGATGTTCCCCGCACCGCGCCAGCAGCATCTGGATGCCCTCGTCCTTCCCGAAG
AGGTCTATCGCGAGCTGGATCGACCCATCCTGCTCGTGCACGGACGCGACGACGTGGTCGTGCCATTGGAAACGAGCCTC
TGGCTCCTCCCGCGGTCGCCGCGGGTGCAACTGCATGTCTTCGGGCAGTGCAGCCACTGGGTACAGATCGAGAAACGCGA
GAGCTTCAACGAGTTGGTCCGTGCGTTTCTGCGCGGCAAGCTCTAA

Upstream 100 bases:

>100_bases
TCGTTTTCTTCGGTGGTGACTTCCGCTGCCTGGAGGTTCAGCTGCACGATACCAGCATGTGGTGGTGGTGAACCGCGCGG
CTGTGGAAGGGAGAGGGGAC

Downstream 100 bases:

>100_bases
GAAGGAGAACACGATGCTGGTCCTGAAGGTGACGATGGTCGAAGGGCGCACGCCGGAACAGAAGGCCGAGTTGATTCGTC
GACTTGCCGAAGCAGCTGGG

Product: putative alpha/beta fold family hydrolase

Products: 2-oxopent-4-enoate; succinate [C]

Alternate protein names: HMSH [H]

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MTIAKSVVHAGRLPTHFHRAGSGDPVIFLHGSGPGVTAWANWRFALPALASDFDCIAPDLWGFARSGHPDPPPIGARAWM
ELWVEQVIALMEHLGIARAHLVGNSMGGAIALHLLYRHRDRFQRVVLMGTVGAPHRMTEQLDVAWGFYEEPTAERLAQII
RWFVYDPATVGGDLEAIARDRLAAALDPVVRRSYEAMFPAPRQQHLDALVLPEEVYRELDRPILLVHGRDDVVVPLETSL
WLLPRSPRVQLHVFGQCSHWVQIEKRESFNELVRAFLRGKL

Sequences:

>Translated_281_residues
MTIAKSVVHAGRLPTHFHRAGSGDPVIFLHGSGPGVTAWANWRFALPALASDFDCIAPDLWGFARSGHPDPPPIGARAWM
ELWVEQVIALMEHLGIARAHLVGNSMGGAIALHLLYRHRDRFQRVVLMGTVGAPHRMTEQLDVAWGFYEEPTAERLAQII
RWFVYDPATVGGDLEAIARDRLAAALDPVVRRSYEAMFPAPRQQHLDALVLPEEVYRELDRPILLVHGRDDVVVPLETSL
WLLPRSPRVQLHVFGQCSHWVQIEKRESFNELVRAFLRGKL
>Mature_280_residues
TIAKSVVHAGRLPTHFHRAGSGDPVIFLHGSGPGVTAWANWRFALPALASDFDCIAPDLWGFARSGHPDPPPIGARAWME
LWVEQVIALMEHLGIARAHLVGNSMGGAIALHLLYRHRDRFQRVVLMGTVGAPHRMTEQLDVAWGFYEEPTAERLAQIIR
WFVYDPATVGGDLEAIARDRLAAALDPVVRRSYEAMFPAPRQQHLDALVLPEEVYRELDRPILLVHGRDDVVVPLETSLW
LLPRSPRVQLHVFGQCSHWVQIEKRESFNELVRAFLRGKL

Specific function: Conversion of 2-hydroxymuconic acid semialdehyde to 2- hydroxypent-2,4-dienoate [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dmpD/todF/xylF esterase family [H]

Homologues:

Organism=Escherichia coli, GI87081721, Length=280, Percent_Identity=30.7142857142857, Blast_Score=114, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: 3.7.1.- [C]

Molecular weight: Translated: 31622; Mature: 31491

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIAKSVVHAGRLPTHFHRAGSGDPVIFLHGSGPGVTAWANWRFALPALASDFDCIAPDL
CCHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCEEEEECCEEECCHHHCCCCCCCHHH
WGFARSGHPDPPPIGARAWMELWVEQVIALMEHLGIARAHLVGNSMGGAIALHLLYRHRD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHH
RFQRVVLMGTVGAPHRMTEQLDVAWGFYEEPTAERLAQIIRWFVYDPATVGGDLEAIARD
HHHEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
RLAAALDPVVRRSYEAMFPAPRQQHLDALVLPEEVYRELDRPILLVHGRDDVVVPLETSL
HHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCHHHHHHCCCCEEEEECCCCEEEEECCCE
WLLPRSPRVQLHVFGQCSHWVQIEKRESFNELVRAFLRGKL
EEECCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TIAKSVVHAGRLPTHFHRAGSGDPVIFLHGSGPGVTAWANWRFALPALASDFDCIAPDL
CHHHHHHHCCCCCHHHHCCCCCCCEEEEECCCCCEEEEECCEEECCHHHCCCCCCCHHH
WGFARSGHPDPPPIGARAWMELWVEQVIALMEHLGIARAHLVGNSMGGAIALHLLYRHRD
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHHHHHHH
RFQRVVLMGTVGAPHRMTEQLDVAWGFYEEPTAERLAQIIRWFVYDPATVGGDLEAIARD
HHHEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
RLAAALDPVVRRSYEAMFPAPRQQHLDALVLPEEVYRELDRPILLVHGRDDVVVPLETSL
HHHHHHHHHHHHHHHHHCCCCHHHCCCEEECCHHHHHHCCCCEEEEECCCCEEEEECCCE
WLLPRSPRVQLHVFGQCSHWVQIEKRESFNELVRAFLRGKL
EEECCCCCEEEEEEECCCCCEEEHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-hydroxy-6-ketononadienedicarboxylate; H2O [C]

Specific reaction: 2-hydroxy-6-ketononadienedicarboxylate + H2O = 2-oxopent-4-enoate + succinate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2194577 [H]