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The map label for this gene is xylJ [H]

Identifier: 221633368

GI number: 221633368

Start: 1379036

End: 1379764

Strand: Reverse

Name: xylJ [H]

Synonym: trd_1388

Alternate gene names: 221633368

Gene position: 1379764-1379036 (Counterclockwise)

Preceding gene: 221633369

Following gene: 221633367

Centisome position: 68.88

GC content: 67.9

Gene sequence:

>729_bases
ATGCTGAGCGAGCCGGAGAAAAAACTCCTGGAAGCGATCCGCGAGGCACGAGAGCGACGCCAGCCTCTCTCGCCGCTCGC
GGAAAAACTCGGGGTCGACCTGGCGGGCGCGTATCGTGTGCAAGCCGCTTCCCTCGCCGACCAGCCCCTCGTGGGGTACA
AGCTGGGGTTGGTGAGCCCGGCGAAGCAGCGTCAGATGGGGCTGAGCAGCCCGATCTATGGTCGGATCAGCCGGACCATG
CTGCGTGAGCAGGTGCTCTCCCTGAGCGAGTTCGTCCAACCGCGCATCGAACCTGAGATCGCGGTCGTCTTGCGTGATCC
GGTCCATCCGGGGGCGCCGCCCGGTGCGATTGCGCTGGCCATCGGGGCGGCCTTTCTGGGCATCGATCTCCTCGACAGCG
TCTGGGAAGGATATCGGTTCAGCGCCCCGGACGTGGTGGCGGACAATGCCTCCGGGGGAGCGTTCTTTCTCGGTGAGCAG
GCGCTGCCCTGGCCGGTCGAGGGTGAACTCTCCCTTTACCTGGATGGCGAACTGGTCGCTGCCGGCTCGATCGCTGCGCT
CGGGCCGGTGACCGAGCATGTCGCGTGGCTGGCGGCGGCAGTCGGTGGTCTCGAGCCGGGTCACGTTCTCTACCTGGGAT
CGCCAGCGGCGGCGGTGCCGGCGAGACCTGGGGTGCTCGAGTTGCGCGGCCCGGGTGGAGCGGTCCTGCTGGCTCGACTG
GAGGCGTGA

Upstream 100 bases:

>100_bases
GAAGTCGGGCGCGATGAGTGGGGCATCGGGACAATGACGATGGCGGAGCGAGACCGTGCTGCCTCGTCGCAGCCAGGAGA
CTGAGGGGGAGTGTCGAGCG

Downstream 100 bases:

>100_bases
CATGGCGGATCAGATGGTGGTGGAGAGCGAGACGGTGGCGCGCCAGCTCGAGCGTGCCTTGACGACGAAGCAGCCGATTC
CCCCACTCACCGAGACACTC

Product: 4-oxalocrotonate decarboxylase

Products: H2O; 2-oxopent-4-enoate [C]

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MLSEPEKKLLEAIREARERRQPLSPLAEKLGVDLAGAYRVQAASLADQPLVGYKLGLVSPAKQRQMGLSSPIYGRISRTM
LREQVLSLSEFVQPRIEPEIAVVLRDPVHPGAPPGAIALAIGAAFLGIDLLDSVWEGYRFSAPDVVADNASGGAFFLGEQ
ALPWPVEGELSLYLDGELVAAGSIAALGPVTEHVAWLAAAVGGLEPGHVLYLGSPAAAVPARPGVLELRGPGGAVLLARL
EA

Sequences:

>Translated_242_residues
MLSEPEKKLLEAIREARERRQPLSPLAEKLGVDLAGAYRVQAASLADQPLVGYKLGLVSPAKQRQMGLSSPIYGRISRTM
LREQVLSLSEFVQPRIEPEIAVVLRDPVHPGAPPGAIALAIGAAFLGIDLLDSVWEGYRFSAPDVVADNASGGAFFLGEQ
ALPWPVEGELSLYLDGELVAAGSIAALGPVTEHVAWLAAAVGGLEPGHVLYLGSPAAAVPARPGVLELRGPGGAVLLARL
EA
>Mature_242_residues
MLSEPEKKLLEAIREARERRQPLSPLAEKLGVDLAGAYRVQAASLADQPLVGYKLGLVSPAKQRQMGLSSPIYGRISRTM
LREQVLSLSEFVQPRIEPEIAVVLRDPVHPGAPPGAIALAIGAAFLGIDLLDSVWEGYRFSAPDVVADNASGGAFFLGEQ
ALPWPVEGELSLYLDGELVAAGSIAALGPVTEHVAWLAAAVGGLEPGHVLYLGSPAAAVPARPGVLELRGPGGAVLLARL
EA

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=167, Percent_Identity=29.940119760479, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 25358; Mature: 25358

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSEPEKKLLEAIREARERRQPLSPLAEKLGVDLAGAYRVQAASLADQPLVGYKLGLVSP
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEECCCCC
AKQRQMGLSSPIYGRISRTMLREQVLSLSEFVQPRIEPEIAVVLRDPVHPGAPPGAIALA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHH
IGAAFLGIDLLDSVWEGYRFSAPDVVADNASGGAFFLGEQALPWPVEGELSLYLDGELVA
HHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCEEE
AGSIAALGPVTEHVAWLAAAVGGLEPGHVLYLGSPAAAVPARPGVLELRGPGGAVLLARL
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEE
EA
CC
>Mature Secondary Structure
MLSEPEKKLLEAIREARERRQPLSPLAEKLGVDLAGAYRVQAASLADQPLVGYKLGLVSP
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEECCCCC
AKQRQMGLSSPIYGRISRTMLREQVLSLSEFVQPRIEPEIAVVLRDPVHPGAPPGAIALA
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHH
IGAAFLGIDLLDSVWEGYRFSAPDVVADNASGGAFFLGEQALPWPVEGELSLYLDGELVA
HHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCEEEECCCCCCCCCCCCEEEEECCCEEE
AGSIAALGPVTEHVAWLAAAVGGLEPGHVLYLGSPAAAVPARPGVLELRGPGGAVLLARL
CCCCHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEEEEEEE
EA
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-ketovalerate [C]

Specific reaction: 4-hydroxy-2-ketovalerate = H2O + 2-oxopent-4-enoate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]