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The map label for this gene is xylJ [H]

Identifier: 221633367

GI number: 221633367

Start: 1378234

End: 1379034

Strand: Reverse

Name: xylJ [H]

Synonym: trd_1387

Alternate gene names: 221633367

Gene position: 1379034-1378234 (Counterclockwise)

Preceding gene: 221633368

Following gene: 221633364

Centisome position: 68.85

GC content: 65.17

Gene sequence:

>801_bases
ATGGCGGATCAGATGGTGGTGGAGAGCGAGACGGTGGCGCGCCAGCTCGAGCGTGCCTTGACGACGAAGCAGCCGATTCC
CCCACTCACCGAGACACTCGGTCCCCTGACGCCGGAACAGGCGTATGCCATCCAGACGCGCTGGACCGAGTTGCGGATCG
CTCAGGGTGAGCGCGTCATCGGTCGCAAGATCGGGTTGACCAGCCGGGCAATGCAAGAACAGTTGGGAGTCCGCGAGCCG
GACTACGGGAGCCTCTGGGCCTCGCGCTTTTTCCCGGCTTGCGGTGGACGAGCCGAGATTCCGACCGAGATCCTCATTCA
GCCACGCCTGGAGGGGGAGATCGCCTTCCTGATCGGTCGCCGCTTGGAGGGACCCGGTGTCACGCTGCAGCAAGTGCTCG
CTGCGACCGAGGCGGTCGCGCTGTCGGTGGAAATCGTCGATAGCCGCATCGAGGCCTGGCGCATCAAACTGGCCGATACC
ATCGCTGACGATGCCTCGTTCGGTGCTTTTACCGTCGGTCCCTGGAGCCGCGCACTCGCCCGCGAGGATCTGCGGACGGT
AGGAATGATCATCCACAAGAACGGCGAGGTCGCGACCGAGGGAATCGGGGCAGCTGCGTTAGGTCATCCGGCTCGCTGCG
TGGCGTGGTTGGCCAACAAGCTCGCCGAATTCGGCATCGCGCTGGAGCCGGGGGATATCGTGCTCTCGGGCTCACTCGCC
CGAGCTCTTCCGGCGGCCCGTGGCGATCTGTTCGTCGTCGAGCTGCATGGTCAGCCACCGCTGACGGTTCGCTTCGTGTG
A

Upstream 100 bases:

>100_bases
TACCTGGGATCGCCAGCGGCGGCGGTGCCGGCGAGACCTGGGGTGCTCGAGTTGCGCGGCCCGGGTGGAGCGGTCCTGCT
GGCTCGACTGGAGGCGTGAC

Downstream 100 bases:

>100_bases
GCGAGACGCTACCAGCGACGCGGTTGGCATCCGGTCTCGTCCGGGAGAAGAGGGCGGACGAGCAGGACGAACTCCTGCTG
GCCGCTCTCCCAACGCGTCA

Product: 2-oxo-hepta-3-ene-1,7-dioic acid hydratase

Products: H2O; 2-oxopent-4-enoate [C]

Alternate protein names: HPH; 2-oxopent-4-enoate hydratase [H]

Number of amino acids: Translated: 266; Mature: 265

Protein sequence:

>266_residues
MADQMVVESETVARQLERALTTKQPIPPLTETLGPLTPEQAYAIQTRWTELRIAQGERVIGRKIGLTSRAMQEQLGVREP
DYGSLWASRFFPACGGRAEIPTEILIQPRLEGEIAFLIGRRLEGPGVTLQQVLAATEAVALSVEIVDSRIEAWRIKLADT
IADDASFGAFTVGPWSRALAREDLRTVGMIIHKNGEVATEGIGAAALGHPARCVAWLANKLAEFGIALEPGDIVLSGSLA
RALPAARGDLFVVELHGQPPLTVRFV

Sequences:

>Translated_266_residues
MADQMVVESETVARQLERALTTKQPIPPLTETLGPLTPEQAYAIQTRWTELRIAQGERVIGRKIGLTSRAMQEQLGVREP
DYGSLWASRFFPACGGRAEIPTEILIQPRLEGEIAFLIGRRLEGPGVTLQQVLAATEAVALSVEIVDSRIEAWRIKLADT
IADDASFGAFTVGPWSRALAREDLRTVGMIIHKNGEVATEGIGAAALGHPARCVAWLANKLAEFGIALEPGDIVLSGSLA
RALPAARGDLFVVELHGQPPLTVRFV
>Mature_265_residues
ADQMVVESETVARQLERALTTKQPIPPLTETLGPLTPEQAYAIQTRWTELRIAQGERVIGRKIGLTSRAMQEQLGVREPD
YGSLWASRFFPACGGRAEIPTEILIQPRLEGEIAFLIGRRLEGPGVTLQQVLAATEAVALSVEIVDSRIEAWRIKLADTI
ADDASFGAFTVGPWSRALAREDLRTVGMIIHKNGEVATEGIGAAALGHPARCVAWLANKLAEFGIALEPGDIVLSGSLAR
ALPAARGDLFVVELHGQPPLTVRFV

Specific function: Conversion of 2-hydroxypent-2,4-dienoate into 4-hydroxy- 2-oxopentanoate [H]

COG id: COG3971

COG function: function code Q; 2-keto-4-pentenoate hydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hydratase/decarboxylase family [H]

Homologues:

Organism=Escherichia coli, GI87081722, Length=248, Percent_Identity=38.7096774193548, Blast_Score=175, Evalue=2e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002529
- InterPro:   IPR011234 [H]

Pfam domain/function: PF01557 FAA_hydrolase [H]

EC number: 4.2.1.- [C]

Molecular weight: Translated: 28760; Mature: 28628

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADQMVVESETVARQLERALTTKQPIPPLTETLGPLTPEQAYAIQTRWTELRIAQGERVI
CCCCEEECHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHEEEEEHHEEEEEECCCCHH
GRKIGLTSRAMQEQLGVREPDYGSLWASRFFPACGGRAEIPTEILIQPRLEGEIAFLIGR
HHHCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCEEEEEEC
RLEGPGVTLQQVLAATEAVALSVEIVDSRIEAWRIKLADTIADDASFGAFTVGPWSRALA
CCCCCCCHHHHHHHHHHHHHEEHHHHHHHHHHHEEEHHHHHCCCCCCCEEEECHHHHHHH
REDLRTVGMIIHKNGEVATEGIGAAALGHPARCVAWLANKLAEFGIALEPGDIVLSGSLA
HHHHHHHEEEEECCCCEECCCCCHHHHCCHHHHHHHHHHHHHHCCCEECCCCEEEECCHH
RALPAARGDLFVVELHGQPPLTVRFV
HHCCCCCCCEEEEEECCCCCEEEEEC
>Mature Secondary Structure 
ADQMVVESETVARQLERALTTKQPIPPLTETLGPLTPEQAYAIQTRWTELRIAQGERVI
CCCEEECHHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCHHEEEEEHHEEEEEECCCCHH
GRKIGLTSRAMQEQLGVREPDYGSLWASRFFPACGGRAEIPTEILIQPRLEGEIAFLIGR
HHHCCCHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCEEEEEEC
RLEGPGVTLQQVLAATEAVALSVEIVDSRIEAWRIKLADTIADDASFGAFTVGPWSRALA
CCCCCCCHHHHHHHHHHHHHEEHHHHHHHHHHHEEEHHHHHCCCCCCCEEEECHHHHHHH
REDLRTVGMIIHKNGEVATEGIGAAALGHPARCVAWLANKLAEFGIALEPGDIVLSGSLA
HHHHHHHEEEEECCCCEECCCCCHHHHCCHHHHHHHHHHHHHHCCCEECCCCEEEECCHH
RALPAARGDLFVVELHGQPPLTVRFV
HHCCCCCCCEEEEEECCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 4-hydroxy-2-ketovalerate [C]

Specific reaction: 4-hydroxy-2-ketovalerate = H2O + 2-oxopent-4-enoate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1791759 [H]