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The map label for this gene is pyrG

Identifier: 221633219

GI number: 221633219

Start: 1220511

End: 1222166

Strand: Reverse

Name: pyrG

Synonym: trd_1236

Alternate gene names: 221633219

Gene position: 1222166-1220511 (Counterclockwise)

Preceding gene: 221633220

Following gene: 221633218

Centisome position: 61.02

GC content: 62.38

Gene sequence:

>1656_bases
ATGCCGAAGTACGTCTTCGTGACGGGGGGTGTTGTCTCATCGGTCGGGAAGGGGATCACCACCGCTGCGATCGGGAGACT
GCTCAAGAGCCGCGGCTTGCGGGTGGCATTGATGAAACTGGATCCCTACTTGAACGTGGATCCGGGCACGATGTCGCCGT
ACCAGCACGGCGAAGTCTTCGTCACCGAAGATGGCGCGGAGACCGATCTCGATCTTGGCCATTATGAGCGGTTCACGGAC
GAGAATTTGAGTCGGGCATCCAACGTCACGACCGGTCAAGTCTACTCGGCCGTGATCGCGAAGGAACGCCGTGGAGATTA
TCTCGGTGGCACCGTTCAGGTGATCCCGCACATCACCAACGAGATCAAGGCACGCATCCGCCAGGTGGCGGAGCTTCACC
GACCGGATGTCATCGTGGTGGAGGTCGGTGGCACAGTTGGTGATATCGAAGGGCAGCCATTCCTGGAGGCGATTCGCCAG
ATGCGGCGCGAGGAAGGCCGCCGCAACGTGCTGTATATCCATGTCACGTTCTTGCCGTACATCGCTAGCACCGGCGAAGT
GAAGACCAAGCCGACCCAACACAGCGTCAAGGAATTGCGCGCGATCGGGATCCAACCGGACGTGATCATCTGTCGGACCG
ATCATCCGGTCGGTGATGACGTGCGGGCGAAGGTCGCGCTCTTCGGCGATGTGGACGAGGATGCCGTCATCTTGTTGCCG
ACGGCCGAGACGATTTACGAAGTTCCCTTGATGCTGGAGCGCGCGGGGCTGGGCCGATACGTCATGGAGCATCTCGGCTG
GGACGATCGCGATCCCGATTTGGCCGACTGGGAGCGGATGGTGGAACGGTTGAAGTCGCCGCGCCGGCGTCTCCGCATCG
CGCTGGTCGGGAAGTACGTCGAGTTGCACGATGCCTACTTGAGCGTGGTCGAAGCCTTGCGCCATGCAGGACTGGCGCAT
GATGTCGCCGTGGAGATCGTCTGGGTCAATTCGACGGCCGAACGAAGCGAGATCGAGGCGGCGCTGCGCCATGTGAACGG
AATCGTGGTGCCGGGAGGGTTCGGCCCGCGCGGCGTCGAAGGGAAGATGCTGGCGGCTCGCTACGCGAGGGAGCGCGGGA
TTCCTTACCTCGGTCTCTGCTACGGCCTGCACATGGCAGTGATCGAGTTCGCGCGAAACGTCCTCGGACTGTGCGGGGCG
AACAGCACGGAAATCGATCCGGAAACGCCCCACCCGGTCATCGACCTCATGCCCGACCAGCGGGGGGTCGAAATGGGGGG
TACGATGCGTTTGGGACGATATCCGTGCCAGCTCGTACCGGGGACCAAGGCAGCGCTCGCGTACGGGGAATCGCTGGTCT
ACGAGCGACACCGCCATCGCTGGGAAGTGAACAATGCCTATCGAGAGGCGTTCGAGGCTGCTGGTTTTGTCGTGAGCGGA
CAATCTCCTGACGGCCGGTACGTCGAGATCATGGAGCTGCACGATCACCCGTGGTTCGTCGGGGTGCAGTTCCATCCCGA
GTTCAAGTCGCGACCGAACCGGCCGCACCCGCTCTTCGTGGCGTTCATCGGTGTGGCCAAGCATGTGTTGCGAGAGGGCG
AGCAGCGACCGCTGCCACTGGCTGAGCCGGTCGCGATGCCTGCTGCCGATGACTGA

Upstream 100 bases:

>100_bases
AGCTTGAGCGTGCGCAAGGGCGAGTTCGGGGAGGCGGTGGAAGTGGTGGTGCTCGTCCCCGATCCCTGACGGTGGAGAGC
GCTGCGCGGGAGGTGTGCTG

Downstream 100 bases:

>100_bases
TCCGTGTTGCTGGTGCCGCTGGGTTTGTGATAGGATGCGCAAGCGTGAGCGGGCCGCAGAGTCGCCCGCCGCGAGTACGA
GATTGGCAAGCGATCGGGAC

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MPKYVFVTGGVVSSVGKGITTAAIGRLLKSRGLRVALMKLDPYLNVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFTD
ENLSRASNVTTGQVYSAVIAKERRGDYLGGTVQVIPHITNEIKARIRQVAELHRPDVIVVEVGGTVGDIEGQPFLEAIRQ
MRREEGRRNVLYIHVTFLPYIASTGEVKTKPTQHSVKELRAIGIQPDVIICRTDHPVGDDVRAKVALFGDVDEDAVILLP
TAETIYEVPLMLERAGLGRYVMEHLGWDDRDPDLADWERMVERLKSPRRRLRIALVGKYVELHDAYLSVVEALRHAGLAH
DVAVEIVWVNSTAERSEIEAALRHVNGIVVPGGFGPRGVEGKMLAARYARERGIPYLGLCYGLHMAVIEFARNVLGLCGA
NSTEIDPETPHPVIDLMPDQRGVEMGGTMRLGRYPCQLVPGTKAALAYGESLVYERHRHRWEVNNAYREAFEAAGFVVSG
QSPDGRYVEIMELHDHPWFVGVQFHPEFKSRPNRPHPLFVAFIGVAKHVLREGEQRPLPLAEPVAMPAADD

Sequences:

>Translated_551_residues
MPKYVFVTGGVVSSVGKGITTAAIGRLLKSRGLRVALMKLDPYLNVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFTD
ENLSRASNVTTGQVYSAVIAKERRGDYLGGTVQVIPHITNEIKARIRQVAELHRPDVIVVEVGGTVGDIEGQPFLEAIRQ
MRREEGRRNVLYIHVTFLPYIASTGEVKTKPTQHSVKELRAIGIQPDVIICRTDHPVGDDVRAKVALFGDVDEDAVILLP
TAETIYEVPLMLERAGLGRYVMEHLGWDDRDPDLADWERMVERLKSPRRRLRIALVGKYVELHDAYLSVVEALRHAGLAH
DVAVEIVWVNSTAERSEIEAALRHVNGIVVPGGFGPRGVEGKMLAARYARERGIPYLGLCYGLHMAVIEFARNVLGLCGA
NSTEIDPETPHPVIDLMPDQRGVEMGGTMRLGRYPCQLVPGTKAALAYGESLVYERHRHRWEVNNAYREAFEAAGFVVSG
QSPDGRYVEIMELHDHPWFVGVQFHPEFKSRPNRPHPLFVAFIGVAKHVLREGEQRPLPLAEPVAMPAADD
>Mature_550_residues
PKYVFVTGGVVSSVGKGITTAAIGRLLKSRGLRVALMKLDPYLNVDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFTDE
NLSRASNVTTGQVYSAVIAKERRGDYLGGTVQVIPHITNEIKARIRQVAELHRPDVIVVEVGGTVGDIEGQPFLEAIRQM
RREEGRRNVLYIHVTFLPYIASTGEVKTKPTQHSVKELRAIGIQPDVIICRTDHPVGDDVRAKVALFGDVDEDAVILLPT
AETIYEVPLMLERAGLGRYVMEHLGWDDRDPDLADWERMVERLKSPRRRLRIALVGKYVELHDAYLSVVEALRHAGLAHD
VAVEIVWVNSTAERSEIEAALRHVNGIVVPGGFGPRGVEGKMLAARYARERGIPYLGLCYGLHMAVIEFARNVLGLCGAN
STEIDPETPHPVIDLMPDQRGVEMGGTMRLGRYPCQLVPGTKAALAYGESLVYERHRHRWEVNNAYREAFEAAGFVVSGQ
SPDGRYVEIMELHDHPWFVGVQFHPEFKSRPNRPHPLFVAFIGVAKHVLREGEQRPLPLAEPVAMPAADD

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Homo sapiens, GI148491070, Length=559, Percent_Identity=46.3327370304114, Blast_Score=497, Evalue=1e-140,
Organism=Homo sapiens, GI28559085, Length=571, Percent_Identity=44.30823117338, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI28559083, Length=571, Percent_Identity=44.30823117338, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI221316689, Length=571, Percent_Identity=44.30823117338, Blast_Score=488, Evalue=1e-138,
Organism=Escherichia coli, GI1789142, Length=536, Percent_Identity=53.1716417910448, Blast_Score=566, Evalue=1e-162,
Organism=Caenorhabditis elegans, GI25148299, Length=608, Percent_Identity=39.6381578947368, Blast_Score=418, Evalue=1e-117,
Organism=Saccharomyces cerevisiae, GI6322563, Length=557, Percent_Identity=44.524236983842, Blast_Score=461, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6319432, Length=563, Percent_Identity=41.0301953818828, Blast_Score=440, Evalue=1e-124,
Organism=Drosophila melanogaster, GI24664469, Length=571, Percent_Identity=45.7092819614711, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI21357815, Length=517, Percent_Identity=44.294003868472, Blast_Score=416, Evalue=1e-116,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRG_THERP (B9L1H7)

Other databases:

- EMBL:   CP001275
- RefSeq:   YP_002522444.1
- ProteinModelPortal:   B9L1H7
- GeneID:   7354584
- GenomeReviews:   CP001275_GR
- KEGG:   tro:trd_1236
- OMA:   RVTMQKL
- ProtClustDB:   PRK05380
- HAMAP:   MF_01227
- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991
- TIGRFAMs:   TIGR00337

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase

EC number: =6.3.4.2

Molecular weight: Translated: 61249; Mature: 61118

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 380-380 ACT_SITE 505-505 ACT_SITE 507-507

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPKYVFVTGGVVSSVGKGITTAAIGRLLKSRGLRVALMKLDPYLNVDPGTMSPYQHGEVF
CCCEEEEECHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTDENLSRASNVTTGQVYSAVIAKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
EIKARIRQVAELHRPDVIVVEVGGTVGDIEGQPFLEAIRQMRREEGRRNVLYIHVTFLPY
HHHHHHHHHHHHCCCCEEEEEECCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEHH
IASTGEVKTKPTQHSVKELRAIGIQPDVIICRTDHPVGDDVRAKVALFGDVDEDAVILLP
HCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCEEEEE
TAETIYEVPLMLERAGLGRYVMEHLGWDDRDPDLADWERMVERLKSPRRRLRIALVGKYV
CHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHEEEHHHHHHH
ELHDAYLSVVEALRHAGLAHDVAVEIVWVNSTAERSEIEAALRHVNGIVVPGGFGPRGVE
HHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCEEECCCCCCCCCC
GKMLAARYARERGIPYLGLCYGLHMAVIEFARNVLGLCGANSTEIDPETPHPVIDLMPDQ
CHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCC
RGVEMGGTMRLGRYPCQLVPGTKAALAYGESLVYERHRHRWEVNNAYREAFEAAGFVVSG
CCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEC
QSPDGRYVEIMELHDHPWFVGVQFHPEFKSRPNRPHPLFVAFIGVAKHVLREGEQRPLPL
CCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCC
AEPVAMPAADD
CCCCCCCCCCC
>Mature Secondary Structure 
PKYVFVTGGVVSSVGKGITTAAIGRLLKSRGLRVALMKLDPYLNVDPGTMSPYQHGEVF
CCEEEEECHHHHHHCCCHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCEEE
VTEDGAETDLDLGHYERFTDENLSRASNVTTGQVYSAVIAKERRGDYLGGTVQVIPHITN
EECCCCCCCCCCHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCCEEEHHHHHH
EIKARIRQVAELHRPDVIVVEVGGTVGDIEGQPFLEAIRQMRREEGRRNVLYIHVTFLPY
HHHHHHHHHHHHCCCCEEEEEECCCEECCCCCHHHHHHHHHHHHCCCCCEEEEEEEEEHH
IASTGEVKTKPTQHSVKELRAIGIQPDVIICRTDHPVGDDVRAKVALFGDVDEDAVILLP
HCCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCCCEEEEEEEECCCCCCEEEEE
TAETIYEVPLMLERAGLGRYVMEHLGWDDRDPDLADWERMVERLKSPRRRLRIALVGKYV
CHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCHHHHEEEHHHHHHH
ELHDAYLSVVEALRHAGLAHDVAVEIVWVNSTAERSEIEAALRHVNGIVVPGGFGPRGVE
HHHHHHHHHHHHHHHCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCEEECCCCCCCCCC
GKMLAARYARERGIPYLGLCYGLHMAVIEFARNVLGLCGANSTEIDPETPHPVIDLMPDQ
CHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCC
RGVEMGGTMRLGRYPCQLVPGTKAALAYGESLVYERHRHRWEVNNAYREAFEAAGFVVSG
CCCCCCCEEEECCCCEEECCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEC
QSPDGRYVEIMELHDHPWFVGVQFHPEFKSRPNRPHPLFVAFIGVAKHVLREGEQRPLPL
CCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCCEEHHHHHHHHHHHHCCCCCCCCC
AEPVAMPAADD
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA