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The map label for this gene is potD [H]

Identifier: 221633130

GI number: 221633130

Start: 1134998

End: 1136176

Strand: Reverse

Name: potD [H]

Synonym: trd_1145

Alternate gene names: 221633130

Gene position: 1136176-1134998 (Counterclockwise)

Preceding gene: 221633135

Following gene: 221633122

Centisome position: 56.72

GC content: 60.05

Gene sequence:

>1179_bases
ATGAGCAACCGTCGAATCTACAGTCGGCGAACAGTGCTCAGGATGCTCGGTGCAGTCTCGGTGATCAGTCCGCTCGCGCT
GAGCTGCCGAAGCAGCGAGCCCGAGGGTGCGACGCCCCGCTCTTCTCCCGCTGCTCGACCGTCCCCAACGGTGGTACCAT
CCTCCGGCTTCCAGTTGCCAGAGTACGTGGACCGGACACGCTTGGAAGGTGAACTCTTCGTCTTCAATTGGGGCGACTAC
ATCGATCCGGATGTGCTGAGTGACTTCGAACGGCTGACCGGTGTCAAGGTGACCGTCGATACGTACGACACCAACGAACA
GGCGATCGCCAAGCTGCAGCAGGGCGGTATTGCCTACGATATCGTCGTGCCGACCGACTACGCGGTGCAGATTCTGATCG
GCCTCGGTCTTCTCGAGCCGCTCGACCGCTCTGTCGTGCGCGCGATCGAGCACCTCGATCCGAACAATCTCAACGGCCCC
TATGATCCGGGAAACCGATACAGCGTGCCGTACTTCTGGGGAACATCCGGTTATGCGTACGATACCGCCGTTCTCGGTCC
GGACCTGGCCAGCTGGGAAGCGCTTTTCGAGCCAGCCGAGGCGGCGCGCGGCAAGATCGTCATGCACGGTTACTACCGGG
AGACGATTGCAGCCGCGCTCTTGTGGCTCGGCTATCCACTGAACGAGACGAGCGATGAGGCTCTGCAGCGAGCCCTCGAG
GTTCTCAAGGCGCAGAAGCCGTTCGTCCTCACGTACACGAGCGAGAACAACGACGAGCTCCTGATCGCCGGCGAAGCAGT
GATCGCGCATTGCTGGACAGGGCAGGCGATCCTAGCCAAGCGAGAGCGGCCGACGATCCGGTATGTCATCCCCAAAGAGG
GGTGTGCGGTCTGGCAGGACAACCTGTGCGTCGTGAAGGGTGCTCCGCATCGCTATGCTGCCATGGTGTTCATCGATTAT
CTCTGTTGGCCGGAAATTGCCGCTCGCAATGCTAGTTATGTCGGATATGCCAGCCCGAATCGGACAGCCCGCGAACAGGG
TTTGCTCGACCCGGATCTGGTGAACGATCCTGCGATCTATCTCGACGATACGACGTGGAAGCGTTTGCAATGGTTCAAAG
ATCTCGGCCCTGATTACGTGAAGTATGACCGGATTTGGACGGAACTTCGCGCAGGCTAG

Upstream 100 bases:

>100_bases
CAACCCTTTCTCGTCCGACGATGCGTCAGTCGCATCGCATGCATAGGATACCGAACCAAGAAACGTAAACGGTCGGGAAA
ATTCCGAAGCGGAGGTGAAC

Downstream 100 bases:

>100_bases
GCGAGATCGGCGTTCGGCTGAGAAGAACCGCAGCTCTCGCGGTGACTGATCAGTTGGCTGGCGGCTGCCCGATCGAGGAT
CCAGAGCACCTGGCCATCAA

Product: putative permidine/putrescine ABC transporter periplasmic spermidine/putrescine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 392; Mature: 391

Protein sequence:

>392_residues
MSNRRIYSRRTVLRMLGAVSVISPLALSCRSSEPEGATPRSSPAARPSPTVVPSSGFQLPEYVDRTRLEGELFVFNWGDY
IDPDVLSDFERLTGVKVTVDTYDTNEQAIAKLQQGGIAYDIVVPTDYAVQILIGLGLLEPLDRSVVRAIEHLDPNNLNGP
YDPGNRYSVPYFWGTSGYAYDTAVLGPDLASWEALFEPAEAARGKIVMHGYYRETIAAALLWLGYPLNETSDEALQRALE
VLKAQKPFVLTYTSENNDELLIAGEAVIAHCWTGQAILAKRERPTIRYVIPKEGCAVWQDNLCVVKGAPHRYAAMVFIDY
LCWPEIAARNASYVGYASPNRTAREQGLLDPDLVNDPAIYLDDTTWKRLQWFKDLGPDYVKYDRIWTELRAG

Sequences:

>Translated_392_residues
MSNRRIYSRRTVLRMLGAVSVISPLALSCRSSEPEGATPRSSPAARPSPTVVPSSGFQLPEYVDRTRLEGELFVFNWGDY
IDPDVLSDFERLTGVKVTVDTYDTNEQAIAKLQQGGIAYDIVVPTDYAVQILIGLGLLEPLDRSVVRAIEHLDPNNLNGP
YDPGNRYSVPYFWGTSGYAYDTAVLGPDLASWEALFEPAEAARGKIVMHGYYRETIAAALLWLGYPLNETSDEALQRALE
VLKAQKPFVLTYTSENNDELLIAGEAVIAHCWTGQAILAKRERPTIRYVIPKEGCAVWQDNLCVVKGAPHRYAAMVFIDY
LCWPEIAARNASYVGYASPNRTAREQGLLDPDLVNDPAIYLDDTTWKRLQWFKDLGPDYVKYDRIWTELRAG
>Mature_391_residues
SNRRIYSRRTVLRMLGAVSVISPLALSCRSSEPEGATPRSSPAARPSPTVVPSSGFQLPEYVDRTRLEGELFVFNWGDYI
DPDVLSDFERLTGVKVTVDTYDTNEQAIAKLQQGGIAYDIVVPTDYAVQILIGLGLLEPLDRSVVRAIEHLDPNNLNGPY
DPGNRYSVPYFWGTSGYAYDTAVLGPDLASWEALFEPAEAARGKIVMHGYYRETIAAALLWLGYPLNETSDEALQRALEV
LKAQKPFVLTYTSENNDELLIAGEAVIAHCWTGQAILAKRERPTIRYVIPKEGCAVWQDNLCVVKGAPHRYAAMVFIDYL
CWPEIAARNASYVGYASPNRTAREQGLLDPDLVNDPAIYLDDTTWKRLQWFKDLGPDYVKYDRIWTELRAG

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=323, Percent_Identity=34.0557275541796, Blast_Score=198, Evalue=5e-52,
Organism=Escherichia coli, GI1787078, Length=346, Percent_Identity=29.4797687861272, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1787711, Length=319, Percent_Identity=26.0188087774295, Blast_Score=82, Evalue=5e-17,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 43865; Mature: 43734

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNRRIYSRRTVLRMLGAVSVISPLALSCRSSEPEGATPRSSPAARPSPTVVPSSGFQLP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCH
EYVDRTRLEGELFVFNWGDYIDPDVLSDFERLTGVKVTVDTYDTNEQAIAKLQQGGIAYD
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEE
IVVPTDYAVQILIGLGLLEPLDRSVVRAIEHLDPNNLNGPYDPGNRYSVPYFWGTSGYAY
EEECCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCEEECCCCCEE
DTAVLGPDLASWEALFEPAEAARGKIVMHGYYRETIAAALLWLGYPLNETSDEALQRALE
EEEECCCCHHHHHHHHCCHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VLKAQKPFVLTYTSENNDELLIAGEAVIAHCWTGQAILAKRERPTIRYVIPKEGCAVWQD
HHHCCCCEEEEEECCCCCEEEEECCEEEEEECCCCHHEEECCCCEEEEEECCCCCEEECC
NLCVVKGAPHRYAAMVFIDYLCWPEIAARNASYVGYASPNRTAREQGLLDPDLVNDPAIY
CEEEEECCCCHHHHHHHHHHHHCHHHHHCCCCEEEECCCCCCHHHCCCCCCCCCCCCEEE
LDDTTWKRLQWFKDLGPDYVKYDRIWTELRAG
EECCHHHHHHHHHHCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNRRIYSRRTVLRMLGAVSVISPLALSCRSSEPEGATPRSSPAARPSPTVVPSSGFQLP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEECCCCCCCH
EYVDRTRLEGELFVFNWGDYIDPDVLSDFERLTGVKVTVDTYDTNEQAIAKLQQGGIAYD
HHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCEEEE
IVVPTDYAVQILIGLGLLEPLDRSVVRAIEHLDPNNLNGPYDPGNRYSVPYFWGTSGYAY
EEECCHHHHHHHHHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCEECCEEECCCCCEE
DTAVLGPDLASWEALFEPAEAARGKIVMHGYYRETIAAALLWLGYPLNETSDEALQRALE
EEEECCCCHHHHHHHHCCHHHHCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHH
VLKAQKPFVLTYTSENNDELLIAGEAVIAHCWTGQAILAKRERPTIRYVIPKEGCAVWQD
HHHCCCCEEEEEECCCCCEEEEECCEEEEEECCCCHHEEECCCCEEEEEECCCCCEEECC
NLCVVKGAPHRYAAMVFIDYLCWPEIAARNASYVGYASPNRTAREQGLLDPDLVNDPAIY
CEEEEECCCCHHHHHHHHHHHHCHHHHHCCCCEEEECCCCCCHHHCCCCCCCCCCCCEEE
LDDTTWKRLQWFKDLGPDYVKYDRIWTELRAG
EECCHHHHHHHHHHCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]