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The map label for this gene is prpE [H]

Identifier: 221632935

GI number: 221632935

Start: 917037

End: 918347

Strand: Reverse

Name: prpE [H]

Synonym: trd_0940

Alternate gene names: 221632935

Gene position: 918347-917037 (Counterclockwise)

Preceding gene: 221632937

Following gene: 221632933

Centisome position: 45.85

GC content: 61.63

Gene sequence:

>1311_bases
ATGATCACGGACGACGAGGCAGATCAGAGTGCGACGCGTGAAGCTTTCGAGCTCTTGCATCGGGTCGCGGAAGGGCGGCT
CCGGCGTGGCCGTCTTACGGTGATCGATGCGACGAATACCCGTCGCGATGCCCGTTTGCCACTGATCGAACTGGCTCGTC
GTTACCACGTGCCCTGCGTGGCGATCATCTTCGATTACCCGCTGGAAGCGTATCTCGCAAACGATCAGCAACGGCCTGGC
CGACAGGTGGGACGAGGTGTCATCGAGCGGCAATTCGCTCAACTGGAGGAGAATCGGGCAGCGCTGGTCGAAGAGCCGTT
CCAACGCCGTTACTGGTTGCGGAGTCCGCAAGAGGCTGCCTCCGCAATCGTTGTTCGTCGACCGTTGCCGAGCGATCGAC
GATGGTGGCGTGGACCCTTCGATATCGTGGGCGATGTGCACGGCTGTTTCGACGAGTTGGTCGAGCTCCTCGAGCACCTC
GGTTACCAGATTGCGGAGCAACGGAATCGTTCTGGCGAGCGACAGTACTTGGTCGCTCATCCTGGAGGGCGACGCCTCGT
TTTCGTCGGAGACTTGGTCGACAGGGGACCGAAGATCGTCCCCGTTCTGCAGCTGGCGATGTCTGCCGTTGCGCGTGGGC
GAGCCGCTGCCGTGATGGGCAATCATGATCGAAAGTTGCTCCGTTACTTGGAGGGGCACCAAGTGCGGATCGCGCACGGT
TTCGAGGCAAGTGTGGAGCAACTTGCCGTAGAGTCGGAGGAGTTTCGGCAACGAGTGTGGCGCTTCCTGCGCAAGCTGCC
GCACCATCTCGTTTTGGACGAAGGGCGGCTCGTCGTCGCCCATGCGGGTTTGCCTGCCGAGTACCATGGTCGCGATTCAC
CGGTGGTGCGACGCATCGCCTTGTTCGGGATGACGACCGGAGAGGTGGACGAGCGCGGATTGCCGATCCGGCTCAATTGG
GCGCTCGATTATCGCGGGCGCGCGCTCGTGGTTTACGGGCACACGCCGGTAACGGAGCCGGTGTGGATCAATCGAACGAT
CAATATCGACACCGGTTGCGTATTCGGCCATCGCCTGACGGCTCTACGGTATCCGGAGTTGGAACTCGTTTCAGTTCCGG
CACGGCATGCGTATGCGGCTCCGCCCCGCCGGTTGCTCACTCCCGATGAATTGACGCAGCCCCTGCTCTTACCATCCGAG
GAGTTGGCAGAGCTGCAGGGTGAGGAACTGGTCGAGGAGACGATTGCCGCTGTTGCCGGGTCACCGGAGTTCGAGCGTGA
CCGTGCACCTGAGAGCGCAGCCCAAGAATGA

Upstream 100 bases:

>100_bases
CCTGGTTCTGCTCGTCGGGCCATCTGGTTCAGGCAAATCAACCTTCGCTCAGCGCCATTTCCGGCCGACAGAGGTGCTTT
CCTCTGATTTCTTCCGTGCT

Downstream 100 bases:

>100_bases
GGTAACAGGGGATCGGAGAACGCAAAACGCTCTCCTCTGGTCAAGGATATTTTCTATTCTTTTTGGTACCCCCTTCGGTA
ATCCATTTTATGGTATCGAG

Product: putative serine/threonine protein phosphatase

Products: NA

Alternate protein names: Ap4A hydrolase; Diadenosine 5',5'''-P1,P4-tetraphosphate asymmetrical hydrolase; Diadenosine tetraphosphatase [H]

Number of amino acids: Translated: 436; Mature: 436

Protein sequence:

>436_residues
MITDDEADQSATREAFELLHRVAEGRLRRGRLTVIDATNTRRDARLPLIELARRYHVPCVAIIFDYPLEAYLANDQQRPG
RQVGRGVIERQFAQLEENRAALVEEPFQRRYWLRSPQEAASAIVVRRPLPSDRRWWRGPFDIVGDVHGCFDELVELLEHL
GYQIAEQRNRSGERQYLVAHPGGRRLVFVGDLVDRGPKIVPVLQLAMSAVARGRAAAVMGNHDRKLLRYLEGHQVRIAHG
FEASVEQLAVESEEFRQRVWRFLRKLPHHLVLDEGRLVVAHAGLPAEYHGRDSPVVRRIALFGMTTGEVDERGLPIRLNW
ALDYRGRALVVYGHTPVTEPVWINRTINIDTGCVFGHRLTALRYPELELVSVPARHAYAAPPRRLLTPDELTQPLLLPSE
ELAELQGEELVEETIAAVAGSPEFERDRAPESAAQE

Sequences:

>Translated_436_residues
MITDDEADQSATREAFELLHRVAEGRLRRGRLTVIDATNTRRDARLPLIELARRYHVPCVAIIFDYPLEAYLANDQQRPG
RQVGRGVIERQFAQLEENRAALVEEPFQRRYWLRSPQEAASAIVVRRPLPSDRRWWRGPFDIVGDVHGCFDELVELLEHL
GYQIAEQRNRSGERQYLVAHPGGRRLVFVGDLVDRGPKIVPVLQLAMSAVARGRAAAVMGNHDRKLLRYLEGHQVRIAHG
FEASVEQLAVESEEFRQRVWRFLRKLPHHLVLDEGRLVVAHAGLPAEYHGRDSPVVRRIALFGMTTGEVDERGLPIRLNW
ALDYRGRALVVYGHTPVTEPVWINRTINIDTGCVFGHRLTALRYPELELVSVPARHAYAAPPRRLLTPDELTQPLLLPSE
ELAELQGEELVEETIAAVAGSPEFERDRAPESAAQE
>Mature_436_residues
MITDDEADQSATREAFELLHRVAEGRLRRGRLTVIDATNTRRDARLPLIELARRYHVPCVAIIFDYPLEAYLANDQQRPG
RQVGRGVIERQFAQLEENRAALVEEPFQRRYWLRSPQEAASAIVVRRPLPSDRRWWRGPFDIVGDVHGCFDELVELLEHL
GYQIAEQRNRSGERQYLVAHPGGRRLVFVGDLVDRGPKIVPVLQLAMSAVARGRAAAVMGNHDRKLLRYLEGHQVRIAHG
FEASVEQLAVESEEFRQRVWRFLRKLPHHLVLDEGRLVVAHAGLPAEYHGRDSPVVRRIALFGMTTGEVDERGLPIRLNW
ALDYRGRALVVYGHTPVTEPVWINRTINIDTGCVFGHRLTALRYPELELVSVPARHAYAAPPRRLLTPDELTQPLLLPSE
ELAELQGEELVEETIAAVAGSPEFERDRAPESAAQE

Specific function: Asymmetrically hydrolyzes Ap4p to yield AMP and ATP [H]

COG id: COG0639

COG function: function code T; Diadenosine tetraphosphatase and related serine/threonine protein phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prpE family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR006186 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: =3.6.1.17 [H]

Molecular weight: Translated: 49561; Mature: 49561

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00191 CYTOCHROME_B5_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDDEADQSATREAFELLHRVAEGRLRRGRLTVIDATNTRRDARLPLIELARRYHVPCV
CCCCCCCHHHHHHHHHHHHHHHHHCHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEE
AIIFDYPLEAYLANDQQRPGRQVGRGVIERQFAQLEENRAALVEEPFQRRYWLRSPQEAA
EEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHH
SAIVVRRPLPSDRRWWRGPFDIVGDVHGCFDELVELLEHLGYQIAEQRNRSGERQYLVAH
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEC
PGGRRLVFVGDLVDRGPKIVPVLQLAMSAVARGRAAAVMGNHDRKLLRYLEGHQVRIAHG
CCCCEEEEEECHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCEEEEECC
FEASVEQLAVESEEFRQRVWRFLRKLPHHLVLDEGRLVVAHAGLPAEYHGRDSPVVRRIA
CHHHHHHHHHCHHHHHHHHHHHHHHCCHHEEECCCCEEEEECCCCCCCCCCCCHHHHHHH
LFGMTTGEVDERGLPIRLNWALDYRGRALVVYGHTPVTEPVWINRTINIDTGCVFGHRLT
HHCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCEEEEEEEECCCCEEECCHHH
ALRYPELELVSVPARHAYAAPPRRLLTPDELTQPLLLPSEELAELQGEELVEETIAAVAG
EEECCCEEEEECCCHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHCC
SPEFERDRAPESAAQE
CCCCHHCCCCHHHCCC
>Mature Secondary Structure
MITDDEADQSATREAFELLHRVAEGRLRRGRLTVIDATNTRRDARLPLIELARRYHVPCV
CCCCCCCHHHHHHHHHHHHHHHHHCHHCCCCEEEEECCCCCCCCCCCHHHHHHHHCCCEE
AIIFDYPLEAYLANDQQRPGRQVGRGVIERQFAQLEENRAALVEEPFQRRYWLRSPQEAA
EEEECCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCHHHHH
SAIVVRRPLPSDRRWWRGPFDIVGDVHGCFDELVELLEHLGYQIAEQRNRSGERQYLVAH
CEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCEEEEEC
PGGRRLVFVGDLVDRGPKIVPVLQLAMSAVARGRAAAVMGNHDRKLLRYLEGHQVRIAHG
CCCCEEEEEECHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCHHHHHHHHCCCEEEEECC
FEASVEQLAVESEEFRQRVWRFLRKLPHHLVLDEGRLVVAHAGLPAEYHGRDSPVVRRIA
CHHHHHHHHHCHHHHHHHHHHHHHHCCHHEEECCCCEEEEECCCCCCCCCCCCHHHHHHH
LFGMTTGEVDERGLPIRLNWALDYRGRALVVYGHTPVTEPVWINRTINIDTGCVFGHRLT
HHCCCCCCCCCCCCCEEEEEEEECCCCEEEEECCCCCCCCEEEEEEEECCCCEEECCHHH
ALRYPELELVSVPARHAYAAPPRRLLTPDELTQPLLLPSEELAELQGEELVEETIAAVAG
EEECCCEEEEECCCHHCCCCCCCCCCCHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHCC
SPEFERDRAPESAAQE
CCCCHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA