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The map label for this gene is gyrB [H]

Identifier: 221632838

GI number: 221632838

Start: 812265

End: 814175

Strand: Reverse

Name: gyrB [H]

Synonym: trd_0832

Alternate gene names: 221632838

Gene position: 814175-812265 (Counterclockwise)

Preceding gene: 221632839

Following gene: 221632830

Centisome position: 40.65

GC content: 57.98

Gene sequence:

>1911_bases
ATGACGAACTCGACGACACGCTACGATGCGAGTGCCATTCAGGTTCTCGAGGGACTCGAGGCTGTCCGGCGCCGCCCCGG
GATGTACATCGGCAGTACCGATATTCGGGGATTGCACCATCTCATCTACGAGATCGTCGACAACAGCGTCGACGAGGCAC
TGGCCGGGTACTGTGATCGGATCGAGGTCACCATCCACCGGGATGGCTCTGTCCGCGTATGGGACGATGGGCGAGGCATT
CCTGTCGATATCCATCCAAAAGTCGGAAAATCGGCGCTGGAAGTCATCATGACGACACTCCATGCCGGAGGAAAATTCGG
TGGTGGTGGTTATAAGGTCTCCGGTGGTCTTCACGGTGTAGGGGCATCGGTCGTCAATGCGCTCTCCGAATGGCTCGAAG
TGACCGTGCGGCGGGACGGCAAAATATACCACCAGCGGTACGAGCGAGGAGTGCCGGTGACGCCCGTTGAGACTATCGGG
GAGACGACGCCCGACGATCACGGAACCGAGACGGTCTTCCTCCCTGATCGGCTGATCTTCCGCTCGCTCGACTACCAGAG
CGACGTCCTCTTGCAACGTTTTCGGGAAGTAGCCTATCTGACGAGCGGATTGACGATTCATTTCGTCGACGAGCGCACCG
ATCTCGATATGACGTTCTACTTCGAGGGAGGTATCGTCTCCTTCGTGCGGCATTTGAACCGGACGAAGGAAGTGCTTCAG
CCGCAACCGTTCTACGTGAAGCGAGAGCAGAACGGTTGCCTGGTAGAAGTCGCGATTCAATACACGGACGGTTATGCCGA
GTCGACACACGCGTTCGCCAATAACATCAACACCATCGATGGTGGGACGCACGTCACAGGGTTCAAGGCAGCACTGACCC
GGGCAATCAACGAATATGCGCGGCGACACGGTCTTCTCAAAGAAAACGATCCCAACCTGAGTGGTGAGGACGTCCGGGAG
GGACTGACAGCGATCATCTCGGTGATGCTTCCCGAGCCTCAATTCGAAGGGCAAACCAAGACCAAACTCGGGAATGCCGA
GGTGGCCGGTATCGTTCAATCGATCGTCTACGAATCGTTCACGAGCTATCTCGAAGAGCATCCTGCTATCGCGCGGCGTA
TCATCGAAAAGTGCTTGACTGCGGCTCGGGCGCGGGAGGCAGCCCGCAAAGCACGCGAACTGGTCCAGCGCAAGGGGGCG
CTCGATACCTTCAGCCTTCCTGGAAAGCTGGCCGACTGCACCGAGCGCGATCCGGCCAAGGCAGAGCTCTATATCGTCGA
AGGCGACTCAGCAGGTGGCTCGGCCAAGCAAGGACGCGATCGCCGCTTTCAGGCGGTGCTTCCGCTCCGGGGCAAGATTC
TCAATGTCGAGAAGGCGCGACTGGATCGGATGCTACATAACGAGGAGATTCGTGCCCTGATTACCGCGCTCGGCACGGGC
ATCGGCGACCAATACGACCGCTCGAAACTGCGTTACCATCGAGTCATTTTGATGACTGATGCAGATGTCGACGGGGCACA
CATTCGAACTTTGTTGCTCACTTTCTTTTTCCGGTACCTCGAGGGACTGATCATCGACGGTCATCTCTATATCGCCCAGC
CACCGCTGTACCGCCTGCAGAGCGGGAAAGAAGTGCACTACGTTTATTCGGACGAGGAGCGCGATGCCATACTCAACCGG
GGTGACGGTAAGACGTGGGATATACAGCGCTACAAGGGACTTGGGGAAATGAATCCTGAGCAGCTTTGGGAGACGACCAT
GGACCCAGCGCGGCGTACGCTGCTCCAGGTGACGATCGAAGATGCCGTGCGGGCGGATGAGACGTTCAACATGCTGATGG
GATCAGCTGTCCCACCGCGCAAGCGCTTCATCCAGGCACACGCCCGGGAGGTACGGAACCTGGATATCTGA

Upstream 100 bases:

>100_bases
CGACAGGGCTGGTCTTGGCCAGCTTGCTGTTCTATCGCGAACGGATCGTGCTCGTTCTCCGTCAGGGTGCGAATGCAAAG
CGAGAAGAGGAATCGCGACG

Downstream 100 bases:

>100_bases
CGAGCGGGGCTGGTGGTCGATGCGAGGCATCGCACCACCTTGCCCCGCTCCTCGCCGACGAGTTCGATCAACGCCCCGCA
ACAGCACGCTTGAGTTGCGT

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 636; Mature: 635

Protein sequence:

>636_residues
MTNSTTRYDASAIQVLEGLEAVRRRPGMYIGSTDIRGLHHLIYEIVDNSVDEALAGYCDRIEVTIHRDGSVRVWDDGRGI
PVDIHPKVGKSALEVIMTTLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVTVRRDGKIYHQRYERGVPVTPVETIG
ETTPDDHGTETVFLPDRLIFRSLDYQSDVLLQRFREVAYLTSGLTIHFVDERTDLDMTFYFEGGIVSFVRHLNRTKEVLQ
PQPFYVKREQNGCLVEVAIQYTDGYAESTHAFANNINTIDGGTHVTGFKAALTRAINEYARRHGLLKENDPNLSGEDVRE
GLTAIISVMLPEPQFEGQTKTKLGNAEVAGIVQSIVYESFTSYLEEHPAIARRIIEKCLTAARAREAARKARELVQRKGA
LDTFSLPGKLADCTERDPAKAELYIVEGDSAGGSAKQGRDRRFQAVLPLRGKILNVEKARLDRMLHNEEIRALITALGTG
IGDQYDRSKLRYHRVILMTDADVDGAHIRTLLLTFFFRYLEGLIIDGHLYIAQPPLYRLQSGKEVHYVYSDEERDAILNR
GDGKTWDIQRYKGLGEMNPEQLWETTMDPARRTLLQVTIEDAVRADETFNMLMGSAVPPRKRFIQAHAREVRNLDI

Sequences:

>Translated_636_residues
MTNSTTRYDASAIQVLEGLEAVRRRPGMYIGSTDIRGLHHLIYEIVDNSVDEALAGYCDRIEVTIHRDGSVRVWDDGRGI
PVDIHPKVGKSALEVIMTTLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVTVRRDGKIYHQRYERGVPVTPVETIG
ETTPDDHGTETVFLPDRLIFRSLDYQSDVLLQRFREVAYLTSGLTIHFVDERTDLDMTFYFEGGIVSFVRHLNRTKEVLQ
PQPFYVKREQNGCLVEVAIQYTDGYAESTHAFANNINTIDGGTHVTGFKAALTRAINEYARRHGLLKENDPNLSGEDVRE
GLTAIISVMLPEPQFEGQTKTKLGNAEVAGIVQSIVYESFTSYLEEHPAIARRIIEKCLTAARAREAARKARELVQRKGA
LDTFSLPGKLADCTERDPAKAELYIVEGDSAGGSAKQGRDRRFQAVLPLRGKILNVEKARLDRMLHNEEIRALITALGTG
IGDQYDRSKLRYHRVILMTDADVDGAHIRTLLLTFFFRYLEGLIIDGHLYIAQPPLYRLQSGKEVHYVYSDEERDAILNR
GDGKTWDIQRYKGLGEMNPEQLWETTMDPARRTLLQVTIEDAVRADETFNMLMGSAVPPRKRFIQAHAREVRNLDI
>Mature_635_residues
TNSTTRYDASAIQVLEGLEAVRRRPGMYIGSTDIRGLHHLIYEIVDNSVDEALAGYCDRIEVTIHRDGSVRVWDDGRGIP
VDIHPKVGKSALEVIMTTLHAGGKFGGGGYKVSGGLHGVGASVVNALSEWLEVTVRRDGKIYHQRYERGVPVTPVETIGE
TTPDDHGTETVFLPDRLIFRSLDYQSDVLLQRFREVAYLTSGLTIHFVDERTDLDMTFYFEGGIVSFVRHLNRTKEVLQP
QPFYVKREQNGCLVEVAIQYTDGYAESTHAFANNINTIDGGTHVTGFKAALTRAINEYARRHGLLKENDPNLSGEDVREG
LTAIISVMLPEPQFEGQTKTKLGNAEVAGIVQSIVYESFTSYLEEHPAIARRIIEKCLTAARAREAARKARELVQRKGAL
DTFSLPGKLADCTERDPAKAELYIVEGDSAGGSAKQGRDRRFQAVLPLRGKILNVEKARLDRMLHNEEIRALITALGTGI
GDQYDRSKLRYHRVILMTDADVDGAHIRTLLLTFFFRYLEGLIIDGHLYIAQPPLYRLQSGKEVHYVYSDEERDAILNRG
DGKTWDIQRYKGLGEMNPEQLWETTMDPARRTLLQVTIEDAVRADETFNMLMGSAVPPRKRFIQAHAREVRNLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=636, Percent_Identity=25.6289308176101, Blast_Score=126, Evalue=6e-29,
Organism=Homo sapiens, GI19913406, Length=607, Percent_Identity=25.2059308072488, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI48994957, Length=558, Percent_Identity=55.5555555555556, Blast_Score=604, Evalue=1e-174,
Organism=Escherichia coli, GI1789408, Length=616, Percent_Identity=42.2077922077922, Blast_Score=471, Evalue=1e-134,
Organism=Caenorhabditis elegans, GI212645845, Length=538, Percent_Identity=26.9516728624535, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI17535065, Length=621, Percent_Identity=25.1207729468599, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI212645657, Length=219, Percent_Identity=28.310502283105, Blast_Score=78, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=621, Percent_Identity=25.4428341384863, Blast_Score=130, Evalue=5e-31,
Organism=Drosophila melanogaster, GI17136538, Length=665, Percent_Identity=25.2631578947368, Blast_Score=142, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 71385; Mature: 71254

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNSTTRYDASAIQVLEGLEAVRRRPGMYIGSTDIRGLHHLIYEIVDNSVDEALAGYCDR
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCHHHHHHCCCCE
IEVTIHRDGSVRVWDDGRGIPVDIHPKVGKSALEVIMTTLHAGGKFGGGGYKVSGGLHGV
EEEEEECCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCH
GASVVNALSEWLEVTVRRDGKIYHQRYERGVPVTPVETIGETTPDDHGTETVFLPDRLIF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHCCCCCCCCCCCEEEEECHHHHH
RSLDYQSDVLLQRFREVAYLTSGLTIHFVDERTDLDMTFYFEGGIVSFVRHLNRTKEVLQ
HHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
PQPFYVKREQNGCLVEVAIQYTDGYAESTHAFANNINTIDGGTHVTGFKAALTRAINEYA
CCCCEEEECCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
RRHGLLKENDPNLSGEDVREGLTAIISVMLPEPQFEGQTKTKLGNAEVAGIVQSIVYESF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHH
TSYLEEHPAIARRIIEKCLTAARAREAARKARELVQRKGALDTFSLPGKLADCTERDPAK
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
AELYIVEGDSAGGSAKQGRDRRFQAVLPLRGKILNVEKARLDRMLHNEEIRALITALGTG
EEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCC
IGDQYDRSKLRYHRVILMTDADVDGAHIRTLLLTFFFRYLEGLIIDGHLYIAQPPLYRLQ
CCCHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCEEEEECCCHHHHC
SGKEVHYVYSDEERDAILNRGDGKTWDIQRYKGLGEMNPEQLWETTMDPARRTLLQVTIE
CCCEEEEEECCCCHHHHHCCCCCCEEEHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHH
DAVRADETFNMLMGSAVPPRKRFIQAHAREVRNLDI
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TNSTTRYDASAIQVLEGLEAVRRRPGMYIGSTDIRGLHHLIYEIVDNSVDEALAGYCDR
CCCCCCCHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHCCHHHHHHCCCCE
IEVTIHRDGSVRVWDDGRGIPVDIHPKVGKSALEVIMTTLHAGGKFGGGGYKVSGGLHGV
EEEEEECCCCEEEEECCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCH
GASVVNALSEWLEVTVRRDGKIYHQRYERGVPVTPVETIGETTPDDHGTETVFLPDRLIF
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCHHHCCCCCCCCCCCEEEEECHHHHH
RSLDYQSDVLLQRFREVAYLTSGLTIHFVDERTDLDMTFYFEGGIVSFVRHLNRTKEVLQ
HHCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEEEEECCCHHHHHHHHHHHHHHHC
PQPFYVKREQNGCLVEVAIQYTDGYAESTHAFANNINTIDGGTHVTGFKAALTRAINEYA
CCCCEEEECCCCCEEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
RRHGLLKENDPNLSGEDVREGLTAIISVMLPEPQFEGQTKTKLGNAEVAGIVQSIVYESF
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCHHHHHHHHHHHHHHH
TSYLEEHPAIARRIIEKCLTAARAREAARKARELVQRKGALDTFSLPGKLADCTERDPAK
HHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
AELYIVEGDSAGGSAKQGRDRRFQAVLPLRGKILNVEKARLDRMLHNEEIRALITALGTG
EEEEEEECCCCCCCCCCCCCCCEEEECCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCC
IGDQYDRSKLRYHRVILMTDADVDGAHIRTLLLTFFFRYLEGLIIDGHLYIAQPPLYRLQ
CCCHHHHHHHEEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCEEECCEEEEECCCHHHHC
SGKEVHYVYSDEERDAILNRGDGKTWDIQRYKGLGEMNPEQLWETTMDPARRTLLQVTIE
CCCEEEEEECCCCHHHHHCCCCCCEEEHHHHCCCCCCCHHHHHHHHHCHHHHHHHHHHHH
DAVRADETFNMLMGSAVPPRKRFIQAHAREVRNLDI
HHHCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]