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The map label for this gene is ilvB [H]

Identifier: 221632827

GI number: 221632827

Start: 799032

End: 800687

Strand: Reverse

Name: ilvB [H]

Synonym: trd_0821

Alternate gene names: 221632827

Gene position: 800687-799032 (Counterclockwise)

Preceding gene: 221632828

Following gene: 221632826

Centisome position: 39.97

GC content: 62.32

Gene sequence:

>1656_bases
ATGAACTCGGTGGAGATTCCGGCGATCCCCTCGGTCAAACGCGAGCGCCAGGAGACGGGTGCTGAGGCACTCGTCCGCGC
CCTCGAGGCAAATGGCGTGGACGTCGTCTTCACCATTCCGGGCGTCCACACGCTCGATCTCTACGCTGCTCTCGAACGAA
GCCAGATTCGCACAGTTCTTCCGCGACACGAGCAGGGCGCCGGCTACATGGCCGACGGGTACGCGCGGGCGAGTGGCCGC
GTCGGTGTTGCCATAACAGTCACTGGTCCAGGACTGACCAACATCGTCACGCCGATTGCCGAGGCATTCGCCGATAGCTC
GCGAGTCCTCGTAATCAGCACGTGTCTCGACCGACCCTACCTCGGCCACTTGGATGGACGGCTGCACGAGATGACGGATC
AGCTCGCCGTGGTCCGTCCAATCGTCAAGTGGGCACACCGGGTGATGAGTGCTGCAGAAATCAGGTCAGCCGTTGCCGAA
GCGTTCCGCCAACTGTATGACGGGCGGCCGCGCCCAGTCTATTTGGAAGTTCCTCTGGATGTCCTGCGTGAACGCACAGC
GATGAGCGATCCGCTCGTCATCGCCGTTCCACCTGCGAAGCCTGATGATCATCTGCTCGACCGAGCAGCGCAGCTGCTGG
CTGACGCGAATCGAGTCTTCGTTTTAGCCGGCGGTGGAGCTGTCTCCGAGCGCAGCAGCCAGCTGCTCAGCCAACTCGCC
AGTGCCATCGGCGCAGTTGTCAGTACGACCGTGACTGGGAAGGGCGCTATCCCCGATGACCACCCGTTGGCCATCGGCGC
CTTCGGCTATCGTTGGATGCCGGACAACCCGGTGAGTGAGCTCATGCGAGGGTCGGACGTAACGATTGCTATCGGAACCG
GCCTGGGCGCACGGACGACCGCGGAAGGGACGATGCCGCTTCCCTCACCGCTGATCCATATCGATATCGATCCTCTGGAG
TTCCATCGGCGTTATCCAGCAGCCGTTGCGCTGCGAGCCGATTGCGCCGAAGCGCTCGCTGGACTCCTCGAGCGAATCGA
AGCAGGTTTCCGCCCACGATCCCGCTGGGAACCGTGGGAGATCGAGAGCGTGCGGCAGCAGTTACTGAGCGTCACCGATG
ACTCGGCGCGCCGCTATCAGCCCTGGCTGATCGCTCTCCGGCGCGCACTTCCGCGCGATGCGTTTACCGCACACGACATG
ACGATGGTCTGCTACGAGGCTGTCCGCGCTTTTCCTGTCTACGTGCCGCGCACCTACGCATTTCCTCGAGGGTTCGGCAC
GCTCGGCTCCGCTCTCCCGATGGCGATCGGTGCAAAATACGCGCGTCCGGATCGTCCGGCAGTCGCGATCTGTGGCGACG
GCGGAATCCAGTTCACTGTGCAGGAACTCGGTACCCTCGCTCAGTATCGGCTACCGGTTATCGTCGTCGTTTTCGACGAC
CGGTCACATACAGCTGTCAAGCGCGCAATGCTGCAGGCTGGAGTCCGACCCCGCGATGTCGATCTCGTAAACCCGGACTT
CGTCGAACTGGCACAGTCCTACAAGCTGAACGCCATCCGCGTGTCGGACCCCGCCGAACTCGAACGAGCAGCCTCCCAAG
CTGTTGTCGATACGAGTCCTTGGCTGATTCACGTGATGTTGGGTACAGAGGCATGA

Upstream 100 bases:

>100_bases
CGTGGAACGACCGGGTGGCCAACACTCTGGCGTTGCACGGAGATCCGCCTTCTCCTACACTGCGGCGCGGGCATGGGAGC
ACTTCGGGAGGCATTTGGAG

Downstream 100 bases:

>100_bases
TCGTGCGTCCCTTCCGCTTCGCGCGCTTGTCCGCACTCGGTATTCCGCACGGTGTGACCAAGCGGGACCCCTCTCTGCCC
GCTTTTGGATCAGTGAGTGG

Product: putative acetolactate synthase large subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 551; Mature: 551

Protein sequence:

>551_residues
MNSVEIPAIPSVKRERQETGAEALVRALEANGVDVVFTIPGVHTLDLYAALERSQIRTVLPRHEQGAGYMADGYARASGR
VGVAITVTGPGLTNIVTPIAEAFADSSRVLVISTCLDRPYLGHLDGRLHEMTDQLAVVRPIVKWAHRVMSAAEIRSAVAE
AFRQLYDGRPRPVYLEVPLDVLRERTAMSDPLVIAVPPAKPDDHLLDRAAQLLADANRVFVLAGGGAVSERSSQLLSQLA
SAIGAVVSTTVTGKGAIPDDHPLAIGAFGYRWMPDNPVSELMRGSDVTIAIGTGLGARTTAEGTMPLPSPLIHIDIDPLE
FHRRYPAAVALRADCAEALAGLLERIEAGFRPRSRWEPWEIESVRQQLLSVTDDSARRYQPWLIALRRALPRDAFTAHDM
TMVCYEAVRAFPVYVPRTYAFPRGFGTLGSALPMAIGAKYARPDRPAVAICGDGGIQFTVQELGTLAQYRLPVIVVVFDD
RSHTAVKRAMLQAGVRPRDVDLVNPDFVELAQSYKLNAIRVSDPAELERAASQAVVDTSPWLIHVMLGTEA

Sequences:

>Translated_551_residues
MNSVEIPAIPSVKRERQETGAEALVRALEANGVDVVFTIPGVHTLDLYAALERSQIRTVLPRHEQGAGYMADGYARASGR
VGVAITVTGPGLTNIVTPIAEAFADSSRVLVISTCLDRPYLGHLDGRLHEMTDQLAVVRPIVKWAHRVMSAAEIRSAVAE
AFRQLYDGRPRPVYLEVPLDVLRERTAMSDPLVIAVPPAKPDDHLLDRAAQLLADANRVFVLAGGGAVSERSSQLLSQLA
SAIGAVVSTTVTGKGAIPDDHPLAIGAFGYRWMPDNPVSELMRGSDVTIAIGTGLGARTTAEGTMPLPSPLIHIDIDPLE
FHRRYPAAVALRADCAEALAGLLERIEAGFRPRSRWEPWEIESVRQQLLSVTDDSARRYQPWLIALRRALPRDAFTAHDM
TMVCYEAVRAFPVYVPRTYAFPRGFGTLGSALPMAIGAKYARPDRPAVAICGDGGIQFTVQELGTLAQYRLPVIVVVFDD
RSHTAVKRAMLQAGVRPRDVDLVNPDFVELAQSYKLNAIRVSDPAELERAASQAVVDTSPWLIHVMLGTEA
>Mature_551_residues
MNSVEIPAIPSVKRERQETGAEALVRALEANGVDVVFTIPGVHTLDLYAALERSQIRTVLPRHEQGAGYMADGYARASGR
VGVAITVTGPGLTNIVTPIAEAFADSSRVLVISTCLDRPYLGHLDGRLHEMTDQLAVVRPIVKWAHRVMSAAEIRSAVAE
AFRQLYDGRPRPVYLEVPLDVLRERTAMSDPLVIAVPPAKPDDHLLDRAAQLLADANRVFVLAGGGAVSERSSQLLSQLA
SAIGAVVSTTVTGKGAIPDDHPLAIGAFGYRWMPDNPVSELMRGSDVTIAIGTGLGARTTAEGTMPLPSPLIHIDIDPLE
FHRRYPAAVALRADCAEALAGLLERIEAGFRPRSRWEPWEIESVRQQLLSVTDDSARRYQPWLIALRRALPRDAFTAHDM
TMVCYEAVRAFPVYVPRTYAFPRGFGTLGSALPMAIGAKYARPDRPAVAICGDGGIQFTVQELGTLAQYRLPVIVVVFDD
RSHTAVKRAMLQAGVRPRDVDLVNPDFVELAQSYKLNAIRVSDPAELERAASQAVVDTSPWLIHVMLGTEA

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=568, Percent_Identity=23.943661971831, Blast_Score=144, Evalue=1e-34,
Organism=Homo sapiens, GI21361361, Length=589, Percent_Identity=24.4482173174873, Blast_Score=127, Evalue=4e-29,
Organism=Escherichia coli, GI87081685, Length=533, Percent_Identity=33.3958724202627, Blast_Score=250, Evalue=2e-67,
Organism=Escherichia coli, GI1790104, Length=553, Percent_Identity=28.0289330922242, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1786717, Length=538, Percent_Identity=28.6245353159851, Blast_Score=184, Evalue=1e-47,
Organism=Escherichia coli, GI1788716, Length=559, Percent_Identity=26.2969588550984, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1787096, Length=545, Percent_Identity=24.0366972477064, Blast_Score=115, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17542570, Length=558, Percent_Identity=25.6272401433692, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17531299, Length=495, Percent_Identity=25.2525252525253, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=495, Percent_Identity=25.2525252525253, Blast_Score=98, Evalue=1e-20,
Organism=Saccharomyces cerevisiae, GI6323755, Length=599, Percent_Identity=26.8781302170284, Blast_Score=190, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6320816, Length=553, Percent_Identity=22.4231464737794, Blast_Score=87, Evalue=7e-18,
Organism=Drosophila melanogaster, GI19922626, Length=552, Percent_Identity=24.4565217391304, Blast_Score=133, Evalue=4e-31,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59869; Mature: 59869

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSVEIPAIPSVKRERQETGAEALVRALEANGVDVVFTIPGVHTLDLYAALERSQIRTVL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
PRHEQGAGYMADGYARASGRVGVAITVTGPGLTNIVTPIAEAFADSSRVLVISTCLDRPY
CCCCCCCCCEECCEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCC
LGHLDGRLHEMTDQLAVVRPIVKWAHRVMSAAEIRSAVAEAFRQLYDGRPRPVYLEVPLD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
VLRERTAMSDPLVIAVPPAKPDDHLLDRAAQLLADANRVFVLAGGGAVSERSSQLLSQLA
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
SAIGAVVSTTVTGKGAIPDDHPLAIGAFGYRWMPDNPVSELMRGSDVTIAIGTGLGARTT
HHHHHHHHHEECCCCCCCCCCCEEEECCCEECCCCCHHHHHHCCCCEEEEEECCCCCCCC
AEGTMPLPSPLIHIDIDPLEFHRRYPAAVALRADCAEALAGLLERIEAGFRPRSRWEPWE
CCCCCCCCCCEEEEECCHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IESVRQQLLSVTDDSARRYQPWLIALRRALPRDAFTAHDMTMVCYEAVRAFPVYVPRTYA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEECCCCCC
FPRGFGTLGSALPMAIGAKYARPDRPAVAICGDGGIQFTVQELGTLAQYRLPVIVVVFDD
CCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECC
RSHTAVKRAMLQAGVRPRDVDLVNPDFVELAQSYKLNAIRVSDPAELERAASQAVVDTSP
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHEECCCC
WLIHVMLGTEA
EEEEEEECCCC
>Mature Secondary Structure
MNSVEIPAIPSVKRERQETGAEALVRALEANGVDVVFTIPGVHTLDLYAALERSQIRTVL
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHC
PRHEQGAGYMADGYARASGRVGVAITVTGPGLTNIVTPIAEAFADSSRVLVISTCLDRPY
CCCCCCCCCEECCEEECCCCEEEEEEEECCCHHHHHHHHHHHHCCCCCEEEEEECCCCCC
LGHLDGRLHEMTDQLAVVRPIVKWAHRVMSAAEIRSAVAEAFRQLYDGRPRPVYLEVPLD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHH
VLRERTAMSDPLVIAVPPAKPDDHLLDRAAQLLADANRVFVLAGGGAVSERSSQLLSQLA
HHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHH
SAIGAVVSTTVTGKGAIPDDHPLAIGAFGYRWMPDNPVSELMRGSDVTIAIGTGLGARTT
HHHHHHHHHEECCCCCCCCCCCEEEECCCEECCCCCHHHHHHCCCCEEEEEECCCCCCCC
AEGTMPLPSPLIHIDIDPLEFHRRYPAAVALRADCAEALAGLLERIEAGFRPRSRWEPWE
CCCCCCCCCCEEEEECCHHHHHCCCCHHEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IESVRQQLLSVTDDSARRYQPWLIALRRALPRDAFTAHDMTMVCYEAVRAFPVYVPRTYA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCEEECCCCCC
FPRGFGTLGSALPMAIGAKYARPDRPAVAICGDGGIQFTVQELGTLAQYRLPVIVVVFDD
CCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHCCCCEEEEEECC
RSHTAVKRAMLQAGVRPRDVDLVNPDFVELAQSYKLNAIRVSDPAELERAASQAVVDTSP
CCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCEEEECCHHHHHHHHHHHEECCCC
WLIHVMLGTEA
EEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]