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The map label for this gene is infB [H]

Identifier: 221632780

GI number: 221632780

Start: 760615

End: 762474

Strand: Reverse

Name: infB [H]

Synonym: trd_0773

Alternate gene names: 221632780

Gene position: 762474-760615 (Counterclockwise)

Preceding gene: 221632781

Following gene: 221632779

Centisome position: 38.07

GC content: 61.77

Gene sequence:

>1860_bases
ATGAGCACCAAAGCGAAAAAGCACGCACCGGTACAAAACAATCGGTCGCCGATGACTGCACAGCGGAGCTCGCCTGCTGC
GAGTGTCGCCCGCGGCCCTGTCGAGGTCGGAAGCGTCATGACGGTAGGCGAGTTGGCCGAGGCCATCGGCGTCAGTGCCG
TCGAGCTCATCAAGGCCTTGATACGCCGGGGCGTGATGGCCAACATCAATCAGCAAATCGATTTCGAGACCGCGGCCAAA
GTCGCCGCCGACTTCGGAGTCGAACTCGTCGAGCGCACTCCTGAAGTAGTCCACCAGGAAAAGAGCCTGGCCGAGATTGT
CCGAGCCAGTGCCCAGGAGCCTGGTGCCGTCCCTCGACCACCCGTGGTGACGATCATGGGTCACGTCGATCACGGGAAGA
CGAAGCTCCTCGATGCGATCCGGCACACCAACGTGGCAGAGGGTGAGGCCGGCGGCATCACCCAGCATATCGGCGCGTAT
CAAGTGGAGGTCCAGGGCCGGAAAATCACCTTTCTCGATACACCCGGTCACGAAGCCTTCACCGCAATGCGCGCTCGCGG
GGCACAAGTCACCGACATCGCCGTCCTGGTGGTTGCCGCCGACGATGGCGTCATGCCCCAGACCCGCGAGGCCGTCGCTC
ACGCTCGTGCCGCCAACGTGCCCATCATCGTCGCCATCAACAAGATCGATTTGCCGACCGCAAATCCGGCCCGTGTCAAG
CAGCAGCTCGCTGAGATCGGTGTCATCGTGGAAGAGTACGGCGGTGACGTGCCGGTCGTCGAGGTTTCCGCGAAGCAGCG
CCTCGGGATCGAAGATCTTTTGGAAATGATCCTGTTGGTCGCCGATATGCACGAACTCAAAGCCAATCCGAATCGGCCCG
CGATCGGGGTCATCATCGAAGCGAAGGTCGATCAGAGACGGGGGCCCGTGGCGACTGTCCTCGTGCAAACCGGGACCTTG
AAACTCAACGATGTCGTTGTCGCTGGTGCCACTTGGGGACGGATCCGCGCGATGTTCGATGATCGCGGGCGCAAAGTGCG
ACGCGCGGAGCCATCCATGCCGGTCGAGATCCTCGGACTGGAGAATGTGCCAGAAGCCGGAGATTACCTGCAAGTCGTCG
AGGATGAACGGACCGCGAAGGAGATCGCCACACAGCGCGCACTCAAGCGTCGCGCTGAAGCTGCCGAGGCCCGGGTTGTC
AAGCTCGACGAGTTCCACAAGGGACTGGAAATTGGTCAGACGCGTGAACTCCGTCTCATCCTGAAGGCCGACGTGCAAGG
AAGTCTGGAGGCGATCCAAAACGCGCTGGCCAAGCTCAACGATGAACTCGAGGGAGTCGGCATCACGGTCGTTCATGCTG
CGACCGGCGCCATCTCCGAGTCGGACGTCATGCTGGCTGCCACGACGGGCGCGATCGTCCTCGGCTTCAATGTTCGACCT
GATGTCGCGGCCCGCCGCGCCGCTGAGGCCTCGGGCGTCGACATCCGGTACTACAACGTGATCTACCATCTCTTGGAAGA
GGTCCGAGCCGGAGTGACTGGACTTCTCGCACCGGAGACGCGCGAGGTCATCGACGGTGTCGCTGAGGTACGCGAGATTT
TCCGCCTGCCCAATCGAGCAGTCGCAGCCGGTCTCTACGTCTTGAACGGGAAGGCACTGCGGAACGCGCGAGTTCGCGTC
ATTCGCGACGGCAAGGTCATTCACGACGGTACCGTCGCATCCCTGCGTCGCTTCAAGGAAGATGTGCGGGAAGTCGCTGC
TGGCTACGAGTGCGGCTTGACCATCGAAAACTTCAATGATCTGCGCGTCGGGGACCAAATCGAGTTCTATCACGTCGAAC
GAGTCCCGCGCGGGCAGTGA

Upstream 100 bases:

>100_bases
CACAGGAGTTTCGTGACGAACTCCTTCGTTGGGCCGATCTGCACATCCGCCGTGACGACCCGCCCGCACCTGAGACAACA
CCCGAACAGGAGGACCAGCT

Downstream 100 bases:

>100_bases
ACGTCTGCCCGCGCGGCTCGCCGCGCGAGGGGAGCCAACGATGCCCAGCTATCGACAAGCTCGCCTGGCCGAGTTCTTGC
GAGACGAGATCGCTGCCATC

Product: translation initiation factor IF-2

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 619; Mature: 618

Protein sequence:

>619_residues
MSTKAKKHAPVQNNRSPMTAQRSSPAASVARGPVEVGSVMTVGELAEAIGVSAVELIKALIRRGVMANINQQIDFETAAK
VAADFGVELVERTPEVVHQEKSLAEIVRASAQEPGAVPRPPVVTIMGHVDHGKTKLLDAIRHTNVAEGEAGGITQHIGAY
QVEVQGRKITFLDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVMPQTREAVAHARAANVPIIVAINKIDLPTANPARVK
QQLAEIGVIVEEYGGDVPVVEVSAKQRLGIEDLLEMILLVADMHELKANPNRPAIGVIIEAKVDQRRGPVATVLVQTGTL
KLNDVVVAGATWGRIRAMFDDRGRKVRRAEPSMPVEILGLENVPEAGDYLQVVEDERTAKEIATQRALKRRAEAAEARVV
KLDEFHKGLEIGQTRELRLILKADVQGSLEAIQNALAKLNDELEGVGITVVHAATGAISESDVMLAATTGAIVLGFNVRP
DVAARRAAEASGVDIRYYNVIYHLLEEVRAGVTGLLAPETREVIDGVAEVREIFRLPNRAVAAGLYVLNGKALRNARVRV
IRDGKVIHDGTVASLRRFKEDVREVAAGYECGLTIENFNDLRVGDQIEFYHVERVPRGQ

Sequences:

>Translated_619_residues
MSTKAKKHAPVQNNRSPMTAQRSSPAASVARGPVEVGSVMTVGELAEAIGVSAVELIKALIRRGVMANINQQIDFETAAK
VAADFGVELVERTPEVVHQEKSLAEIVRASAQEPGAVPRPPVVTIMGHVDHGKTKLLDAIRHTNVAEGEAGGITQHIGAY
QVEVQGRKITFLDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVMPQTREAVAHARAANVPIIVAINKIDLPTANPARVK
QQLAEIGVIVEEYGGDVPVVEVSAKQRLGIEDLLEMILLVADMHELKANPNRPAIGVIIEAKVDQRRGPVATVLVQTGTL
KLNDVVVAGATWGRIRAMFDDRGRKVRRAEPSMPVEILGLENVPEAGDYLQVVEDERTAKEIATQRALKRRAEAAEARVV
KLDEFHKGLEIGQTRELRLILKADVQGSLEAIQNALAKLNDELEGVGITVVHAATGAISESDVMLAATTGAIVLGFNVRP
DVAARRAAEASGVDIRYYNVIYHLLEEVRAGVTGLLAPETREVIDGVAEVREIFRLPNRAVAAGLYVLNGKALRNARVRV
IRDGKVIHDGTVASLRRFKEDVREVAAGYECGLTIENFNDLRVGDQIEFYHVERVPRGQ
>Mature_618_residues
STKAKKHAPVQNNRSPMTAQRSSPAASVARGPVEVGSVMTVGELAEAIGVSAVELIKALIRRGVMANINQQIDFETAAKV
AADFGVELVERTPEVVHQEKSLAEIVRASAQEPGAVPRPPVVTIMGHVDHGKTKLLDAIRHTNVAEGEAGGITQHIGAYQ
VEVQGRKITFLDTPGHEAFTAMRARGAQVTDIAVLVVAADDGVMPQTREAVAHARAANVPIIVAINKIDLPTANPARVKQ
QLAEIGVIVEEYGGDVPVVEVSAKQRLGIEDLLEMILLVADMHELKANPNRPAIGVIIEAKVDQRRGPVATVLVQTGTLK
LNDVVVAGATWGRIRAMFDDRGRKVRRAEPSMPVEILGLENVPEAGDYLQVVEDERTAKEIATQRALKRRAEAAEARVVK
LDEFHKGLEIGQTRELRLILKADVQGSLEAIQNALAKLNDELEGVGITVVHAATGAISESDVMLAATTGAIVLGFNVRPD
VAARRAAEASGVDIRYYNVIYHLLEEVRAGVTGLLAPETREVIDGVAEVREIFRLPNRAVAAGLYVLNGKALRNARVRVI
RDGKVIHDGTVASLRRFKEDVREVAAGYECGLTIENFNDLRVGDQIEFYHVERVPRGQ

Specific function: One of the essential components for the initiation of protein synthesis. Protects formylmethionyl-tRNA from spontaneous hydrolysis and promotes its binding to the 30S ribosomal subunits. Also involved in the hydrolysis of GTP during the formation of the 7

COG id: COG0532

COG function: function code J; Translation initiation factor 2 (IF-2; GTPase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IF-2 family [H]

Homologues:

Organism=Homo sapiens, GI53729339, Length=645, Percent_Identity=37.2093023255814, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI53729337, Length=645, Percent_Identity=37.2093023255814, Blast_Score=379, Evalue=1e-105,
Organism=Homo sapiens, GI84043963, Length=130, Percent_Identity=45.3846153846154, Blast_Score=119, Evalue=9e-27,
Organism=Homo sapiens, GI157426893, Length=231, Percent_Identity=26.8398268398268, Blast_Score=79, Evalue=1e-14,
Organism=Escherichia coli, GI1789559, Length=597, Percent_Identity=51.4237855946399, Blast_Score=549, Evalue=1e-157,
Organism=Escherichia coli, GI2367247, Length=217, Percent_Identity=33.6405529953917, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1788922, Length=234, Percent_Identity=29.9145299145299, Blast_Score=80, Evalue=5e-16,
Organism=Escherichia coli, GI1790412, Length=126, Percent_Identity=38.8888888888889, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI1789737, Length=126, Percent_Identity=38.8888888888889, Blast_Score=73, Evalue=7e-14,
Organism=Escherichia coli, GI48994988, Length=161, Percent_Identity=34.1614906832298, Blast_Score=73, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994658, Length=626, Percent_Identity=35.7827476038339, Blast_Score=326, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI212656558, Length=558, Percent_Identity=26.5232974910394, Blast_Score=120, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17557151, Length=217, Percent_Identity=31.3364055299539, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=168, Percent_Identity=36.9047619047619, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI25141371, Length=169, Percent_Identity=32.5443786982249, Blast_Score=70, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324550, Length=535, Percent_Identity=39.0654205607477, Blast_Score=340, Evalue=3e-94,
Organism=Saccharomyces cerevisiae, GI6319282, Length=130, Percent_Identity=46.1538461538462, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6324761, Length=159, Percent_Identity=35.8490566037736, Blast_Score=75, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323320, Length=270, Percent_Identity=26.2962962962963, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI28572034, Length=654, Percent_Identity=36.3914373088685, Blast_Score=360, Evalue=2e-99,
Organism=Drosophila melanogaster, GI160714833, Length=71, Percent_Identity=73.2394366197183, Blast_Score=114, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24656849, Length=130, Percent_Identity=43.8461538461538, Blast_Score=112, Evalue=1e-24,
Organism=Drosophila melanogaster, GI78706572, Length=254, Percent_Identity=29.5275590551181, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281363316, Length=165, Percent_Identity=34.5454545454545, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17864358, Length=165, Percent_Identity=34.5454545454545, Blast_Score=73, Evalue=5e-13,
Organism=Drosophila melanogaster, GI19922690, Length=228, Percent_Identity=28.9473684210526, Blast_Score=69, Evalue=1e-11,
Organism=Drosophila melanogaster, GI19921738, Length=129, Percent_Identity=40.3100775193798, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1150 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006847
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000178
- InterPro:   IPR015760
- InterPro:   IPR023115
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF11987 IF-2; PF04760 IF2_N [H]

EC number: NA

Molecular weight: Translated: 66812; Mature: 66680

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS01176 IF2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKAKKHAPVQNNRSPMTAQRSSPAASVARGPVEVGSVMTVGELAEAIGVSAVELIKAL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCHHHHHHHHHHCHHHHHHHHHH
IRRGVMANINQQIDFETAAKVAADFGVELVERTPEVVHQEKSLAEIVRASAQEPGAVPRP
HHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
PVVTIMGHVDHGKTKLLDAIRHTNVAEGEAGGITQHIGAYQVEVQGRKITFLDTPGHEAF
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEEECCCEEEEEECCCHHHH
TAMRARGAQVTDIAVLVVAADDGVMPQTREAVAHARAANVPIIVAINKIDLPTANPARVK
HHHHHCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
QQLAEIGVIVEEYGGDVPVVEVSAKQRLGIEDLLEMILLVADMHELKANPNRPAIGVIIE
HHHHHHHHHHHHCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
AKVDQRRGPVATVLVQTGTLKLNDVVVAGATWGRIRAMFDDRGRKVRRAEPSMPVEILGL
ECCCCCCCCEEEEEEEECEEEECCEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEEEC
ENVPEAGDYLQVVEDERTAKEIATQRALKRRAEAAEARVVKLDEFHKGLEIGQTRELRLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCCCCCEEEEE
LKADVQGSLEAIQNALAKLNDELEGVGITVVHAATGAISESDVMLAATTGAIVLGFNVRP
EEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEECCEEEEEECCCC
DVAARRAAEASGVDIRYYNVIYHLLEEVRAGVTGLLAPETREVIDGVAEVREIFRLPNRA
CHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHH
VAAGLYVLNGKALRNARVRVIRDGKVIHDGTVASLRRFKEDVREVAAGYECGLTIENFND
HHHEEEEECCCHHCCCEEEEEECCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
LRVGDQIEFYHVERVPRGQ
CCCCCEEEEEEECCCCCCC
>Mature Secondary Structure 
STKAKKHAPVQNNRSPMTAQRSSPAASVARGPVEVGSVMTVGELAEAIGVSAVELIKAL
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCHHHCCCHHHHHHHHHHCHHHHHHHHHH
IRRGVMANINQQIDFETAAKVAADFGVELVERTPEVVHQEKSLAEIVRASAQEPGAVPRP
HHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCC
PVVTIMGHVDHGKTKLLDAIRHTNVAEGEAGGITQHIGAYQVEVQGRKITFLDTPGHEAF
CEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHCCEEEEEECCCEEEEEECCCHHHH
TAMRARGAQVTDIAVLVVAADDGVMPQTREAVAHARAANVPIIVAINKIDLPTANPARVK
HHHHHCCCEEEEEEEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHH
QQLAEIGVIVEEYGGDVPVVEVSAKQRLGIEDLLEMILLVADMHELKANPNRPAIGVIIE
HHHHHHHHHHHHCCCCCCEEEECCHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEE
AKVDQRRGPVATVLVQTGTLKLNDVVVAGATWGRIRAMFDDRGRKVRRAEPSMPVEILGL
ECCCCCCCCEEEEEEEECEEEECCEEEECCCHHHHHHHHHHCCCEECCCCCCCCEEEEEC
ENVPEAGDYLQVVEDERTAKEIATQRALKRRAEAAEARVVKLDEFHKGLEIGQTRELRLI
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEHHHHHCCCCCCCCCEEEEE
LKADVQGSLEAIQNALAKLNDELEGVGITVVHAATGAISESDVMLAATTGAIVLGFNVRP
EEECCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEECCEEEEEECCCC
DVAARRAAEASGVDIRYYNVIYHLLEEVRAGVTGLLAPETREVIDGVAEVREIFRLPNRA
CHHHHHHCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHH
VAAGLYVLNGKALRNARVRVIRDGKVIHDGTVASLRRFKEDVREVAAGYECGLTIENFND
HHHEEEEECCCHHCCCEEEEEECCEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEECCCC
LRVGDQIEFYHVERVPRGQ
CCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA