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The map label for this gene is rfaQ [H]

Identifier: 221632676

GI number: 221632676

Start: 654231

End: 655388

Strand: Reverse

Name: rfaQ [H]

Synonym: trd_0659

Alternate gene names: 221632676

Gene position: 655388-654231 (Counterclockwise)

Preceding gene: 221632677

Following gene: 221632675

Centisome position: 32.72

GC content: 63.9

Gene sequence:

>1158_bases
ATGACGCGAGACTTCGTGCGCCGGCTCGTGCGTCGGTTGGGATCCACAGTCCCTGCTCTTCCCCTCGCACCGAGCACTCG
ACGCGAAGTCATCCTGATTCGTCCTGACCACCTGGGCGATGCACTCCTGACGATTCCCGCCTTGCGTGCTCTCCGGGCCA
CCGAGCACCGGTTGTACCTGACGCTCCTCGTCGGGCCATGGACCCGAGAGGTCTTCATGCTTCGCGGGCTGGTCGACGAG
GTACTCGTTGCGTCGTTTCCAGGCTTTCGCCGAGAGCCCACTCGAAATCCGTTCGAGCCCTATAGACTGCTCTTTCGACT
CGCTGGCAGTCTCCGACGACGCGGTCCCATGGCCCTGGTCGTCCTGCGCGATGATCACTGGTGGGGAGCCTGGCTCGGCG
CACTGGCCGGTGTTCCGGTACGGGTCGGAGCGGATCATCCTGCTATCCGCCCGTTTTTGACTCACCCAATCCCTCTCCAG
AGCCAGCATGTCGCGGCGCGAAATCTCGAATTGGTGACTGCACTGTTGACAGTACTGGGATACACCGACGATTCTCGCCC
GCTGGCACCGGAGGCCTATCCATTGGTATGGCCATCCGATCCAGTCGCCGCACGAGCTGTCCGGGATGTCCTCGAACGCT
GTTGCGTTACAGCTCCCTACATCGTCGTGCATCCGGGTAGTGGAGCCGCCGCCAAGTGCTGGCCGGCGGAACGGTGGGCA
ACGCTCGTTGACACCCTCGCCGAACGGGGTTACCAGGTGCTGCTCACCGGCTCCGCCTCCGAGCATGCGCTCCTCGCCAC
GATCGTTGCCGCGACCAGCAGCGCTCCTCATAACCTCGCGGGACAGCTCTCCCTTCCAGAACTAGCCGAGTTGTTGCGGA
AAGCAGCGCTCGTCATCGGAACCGATACGGGTCCTCTCCACCTCGCCGTCGCCGTGGGTAGCCCAACCGTCCATCTCTTC
GGTCCGACCCGACCCGAGCGGTTCGGACCATGGGGTCCTAGAGCCCGACATCGGGTCGTCCAGGCGCAGTTGCGCTGCCC
GCGCTGCGGCGATATCGGACCGGATCGCGCATGCGGTACTGCCTGTATGATGGCGATCGACGTCGACGATGTGCTGGGAG
CAGCCCTGGAGCTCCTCGAGAGTCGTTCTATCCGATGA

Upstream 100 bases:

>100_bases
GCCTCTCGACGATCGCGGATCGCCTGGACATCAGTCAGGTCCACGTGGACCAAGTTGTCGAGGCTGCGTCTCGGCTTCTG
CAAGGGGAGCAGGGGACCGC

Downstream 100 bases:

>100_bases
GTACTCTGCTGATCGGTATCGACGCCAGCCGCGCTTGTCGCGAACAGATGACAGGAACCGAGCGATACGCTCGACGTGTG
ATCGACGGCTTGCTCACGTT

Product: lipopolysaccharide core biosynthesis heptosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTRDFVRRLVRRLGSTVPALPLAPSTRREVILIRPDHLGDALLTIPALRALRATEHRLYLTLLVGPWTREVFMLRGLVDE
VLVASFPGFRREPTRNPFEPYRLLFRLAGSLRRRGPMALVVLRDDHWWGAWLGALAGVPVRVGADHPAIRPFLTHPIPLQ
SQHVAARNLELVTALLTVLGYTDDSRPLAPEAYPLVWPSDPVAARAVRDVLERCCVTAPYIVVHPGSGAAAKCWPAERWA
TLVDTLAERGYQVLLTGSASEHALLATIVAATSSAPHNLAGQLSLPELAELLRKAALVIGTDTGPLHLAVAVGSPTVHLF
GPTRPERFGPWGPRARHRVVQAQLRCPRCGDIGPDRACGTACMMAIDVDDVLGAALELLESRSIR

Sequences:

>Translated_385_residues
MTRDFVRRLVRRLGSTVPALPLAPSTRREVILIRPDHLGDALLTIPALRALRATEHRLYLTLLVGPWTREVFMLRGLVDE
VLVASFPGFRREPTRNPFEPYRLLFRLAGSLRRRGPMALVVLRDDHWWGAWLGALAGVPVRVGADHPAIRPFLTHPIPLQ
SQHVAARNLELVTALLTVLGYTDDSRPLAPEAYPLVWPSDPVAARAVRDVLERCCVTAPYIVVHPGSGAAAKCWPAERWA
TLVDTLAERGYQVLLTGSASEHALLATIVAATSSAPHNLAGQLSLPELAELLRKAALVIGTDTGPLHLAVAVGSPTVHLF
GPTRPERFGPWGPRARHRVVQAQLRCPRCGDIGPDRACGTACMMAIDVDDVLGAALELLESRSIR
>Mature_384_residues
TRDFVRRLVRRLGSTVPALPLAPSTRREVILIRPDHLGDALLTIPALRALRATEHRLYLTLLVGPWTREVFMLRGLVDEV
LVASFPGFRREPTRNPFEPYRLLFRLAGSLRRRGPMALVVLRDDHWWGAWLGALAGVPVRVGADHPAIRPFLTHPIPLQS
QHVAARNLELVTALLTVLGYTDDSRPLAPEAYPLVWPSDPVAARAVRDVLERCCVTAPYIVVHPGSGAAAKCWPAERWAT
LVDTLAERGYQVLLTGSASEHALLATIVAATSSAPHNLAGQLSLPELAELLRKAALVIGTDTGPLHLAVAVGSPTVHLFG
PTRPERFGPWGPRARHRVVQAQLRCPRCGDIGPDRACGTACMMAIDVDDVLGAALELLESRSIR

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=303, Percent_Identity=27.3927392739274, Blast_Score=105, Evalue=5e-24,
Organism=Escherichia coli, GI1790050, Length=321, Percent_Identity=25.8566978193146, Blast_Score=76, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 42054; Mature: 41922

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRDFVRRLVRRLGSTVPALPLAPSTRREVILIRPDHLGDALLTIPALRALRATEHRLYL
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEE
TLLVGPWTREVFMLRGLVDEVLVASFPGFRREPTRNPFEPYRLLFRLAGSLRRRGPMALV
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEE
VLRDDHWWGAWLGALAGVPVRVGADHPAIRPFLTHPIPLQSQHVAARNLELVTALLTVLG
EEECCCCHHHHHHHHCCCCEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
YTDDSRPLAPEAYPLVWPSDPVAARAVRDVLERCCVTAPYIVVHPGSGAAAKCWPAERWA
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHH
TLVDTLAERGYQVLLTGSASEHALLATIVAATSSAPHNLAGQLSLPELAELLRKAALVIG
HHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCEEEEE
TDTGPLHLAVAVGSPTVHLFGPTRPERFGPWGPRARHRVVQAQLRCPRCGDIGPDRACGT
CCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
ACMMAIDVDDVLGAALELLESRSIR
HEEEEECHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TRDFVRRLVRRLGSTVPALPLAPSTRREVILIRPDHLGDALLTIPALRALRATEHRLYL
CHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCEEEE
TLLVGPWTREVFMLRGLVDEVLVASFPGFRREPTRNPFEPYRLLFRLAGSLRRRGPMALV
EEEECCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEE
VLRDDHWWGAWLGALAGVPVRVGADHPAIRPFLTHPIPLQSQHVAARNLELVTALLTVLG
EEECCCCHHHHHHHHCCCCEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHC
YTDDSRPLAPEAYPLVWPSDPVAARAVRDVLERCCVTAPYIVVHPGSGAAAKCWPAERWA
CCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHH
TLVDTLAERGYQVLLTGSASEHALLATIVAATSSAPHNLAGQLSLPELAELLRKAALVIG
HHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHCEEEEE
TDTGPLHLAVAVGSPTVHLFGPTRPERFGPWGPRARHRVVQAQLRCPRCGDIGPDRACGT
CCCCCEEEEEEECCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHH
ACMMAIDVDDVLGAALELLESRSIR
HEEEEECHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 1447141; 8041620; 9278503 [H]