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The map label for this gene is rfaQ [H]

Identifier: 221632674

GI number: 221632674

Start: 652019

End: 653131

Strand: Reverse

Name: rfaQ [H]

Synonym: trd_0657

Alternate gene names: 221632674

Gene position: 653131-652019 (Counterclockwise)

Preceding gene: 221632675

Following gene: 221632673

Centisome position: 32.61

GC content: 64.24

Gene sequence:

>1113_bases
ATGAGAATCGGACGCTTCGAACGGATGCTCCTCATCAAGATCGCTGATCTCGGTGATGCCGCCATTACCTTGCCGGCCAT
CCACTCGCTGCGAAAGGCGTATCCGCAGGCGTCGCTCCACGTCGTCAGTTCGCCGCTCGGTACCGAACTCTACCGGCTTT
CCCCTGACATCGACCGCTGCTTCGTACTGGAGAAGTCGCGCCTCAAACGTCCCGCTGGAGCCCTCGCCCTGGCTCGTCTC
CTGTGGACGCTCCGCCGGCAGCAGTACGACGCGGTCGCACTGTTCCACCATCTCACGACCAACGCCGGACGCTCGCTCTA
TCGGCTGCTTCTGGCTGCGACCGCCAGTCCGGTCCGGGCAGGTCTCGACAACGGTTTAGGGACTTTTTTGACTCACCGGG
TTCCCGATCGGGGCTTCGGTGCCTGCCCCGAGTGGGACTACGCTCGGGCCGTCGTCGAAGCACTCGGAGCGAGCCCTCTA
CTCGAACGACCACGCCTCCGAGTCCCAGATGCCGCCTTCCGACGAGCCCAGGAGCTCGTCGGTGATGCACGAGATGGCCT
CGTTGTCATCCATCCGGGCGTCGGCCCATTCGCTCCAGCGCGACGCTGGCCGATCGAGTCGTTTACGACCGTGGCACGCG
AACTCACCCGACAGGGTTACCAGGTCGTGGTCACCGGAACAGTCAATGAGGAACCCGAAGCCAGACCATTGCTGCGGACT
CCCGGGGTGACGAGTTTGATCGGCCGAACCGACCTCGCGACTCTGGTCGCCGTTCTGCAGCAGGCCAAACTCGTGATCGG
CAACGACTCGGGAGTCACCCACCTCGCGGCCGTGCTGGGTCGTCCCACGGTGGCCATCTTCGGTCCCTCGAATCCCGATG
CCTGGCGCCCTTTCGAGGCCGAGATCGTCACCCCAGAAACACTGACGACCACGCGGGCTTCGGTGCTGGCTCTCACGGCC
GCGCTCCCCTGCGCACCCTGCTGCTATGTCGGTTTCCGCGTTGGTCGACCCCAGGGCTGTTCCACCCGGACATGCCTGAT
CGATCTTCATCCCGATGTCGTGCTGCGTGCCGCACTGCAACTTCTCGAGGGAGATCGCGACTCCTCCTGGTAA

Upstream 100 bases:

>100_bases
CGCGACTCGTCGCCGCGGGGCAGGAACATGCCCGGCGCTTTACTTGGCATCGCACAGCCAACGAAACGCTCGCAGTTCTG
CTCGAAGCGGTGGGCACTCC

Downstream 100 bases:

>100_bases
GAAACCGTCGAGCGTTGCGCTATCGAGGCCCTTTGCTCCCGTCTTGCAGGCTCGTTGGGCCAAAACAGCGTGCATGAAAC
AGGCAGTCGCAGCGAGCGCG

Product: putative heptosyltransferase III WaaQ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MRIGRFERMLLIKIADLGDAAITLPAIHSLRKAYPQASLHVVSSPLGTELYRLSPDIDRCFVLEKSRLKRPAGALALARL
LWTLRRQQYDAVALFHHLTTNAGRSLYRLLLAATASPVRAGLDNGLGTFLTHRVPDRGFGACPEWDYARAVVEALGASPL
LERPRLRVPDAAFRRAQELVGDARDGLVVIHPGVGPFAPARRWPIESFTTVARELTRQGYQVVVTGTVNEEPEARPLLRT
PGVTSLIGRTDLATLVAVLQQAKLVIGNDSGVTHLAAVLGRPTVAIFGPSNPDAWRPFEAEIVTPETLTTTRASVLALTA
ALPCAPCCYVGFRVGRPQGCSTRTCLIDLHPDVVLRAALQLLEGDRDSSW

Sequences:

>Translated_370_residues
MRIGRFERMLLIKIADLGDAAITLPAIHSLRKAYPQASLHVVSSPLGTELYRLSPDIDRCFVLEKSRLKRPAGALALARL
LWTLRRQQYDAVALFHHLTTNAGRSLYRLLLAATASPVRAGLDNGLGTFLTHRVPDRGFGACPEWDYARAVVEALGASPL
LERPRLRVPDAAFRRAQELVGDARDGLVVIHPGVGPFAPARRWPIESFTTVARELTRQGYQVVVTGTVNEEPEARPLLRT
PGVTSLIGRTDLATLVAVLQQAKLVIGNDSGVTHLAAVLGRPTVAIFGPSNPDAWRPFEAEIVTPETLTTTRASVLALTA
ALPCAPCCYVGFRVGRPQGCSTRTCLIDLHPDVVLRAALQLLEGDRDSSW
>Mature_370_residues
MRIGRFERMLLIKIADLGDAAITLPAIHSLRKAYPQASLHVVSSPLGTELYRLSPDIDRCFVLEKSRLKRPAGALALARL
LWTLRRQQYDAVALFHHLTTNAGRSLYRLLLAATASPVRAGLDNGLGTFLTHRVPDRGFGACPEWDYARAVVEALGASPL
LERPRLRVPDAAFRRAQELVGDARDGLVVIHPGVGPFAPARRWPIESFTTVARELTRQGYQVVVTGTVNEEPEARPLLRT
PGVTSLIGRTDLATLVAVLQQAKLVIGNDSGVTHLAAVLGRPTVAIFGPSNPDAWRPFEAEIVTPETLTTTRASVLALTA
ALPCAPCCYVGFRVGRPQGCSTRTCLIDLHPDVVLRAALQLLEGDRDSSW

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=103, Percent_Identity=36.8932038834951, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1790062, Length=306, Percent_Identity=24.8366013071895, Blast_Score=73, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 40244; Mature: 40244

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIGRFERMLLIKIADLGDAAITLPAIHSLRKAYPQASLHVVSSPLGTELYRLSPDIDRC
CCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHEECCCCCHHE
FVLEKSRLKRPAGALALARLLWTLRRQQYDAVALFHHLTTNAGRSLYRLLLAATASPVRA
EEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GLDNGLGTFLTHRVPDRGFGACPEWDYARAVVEALGASPLLERPRLRVPDAAFRRAQELV
HHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHH
GDARDGLVVIHPGVGPFAPARRWPIESFTTVARELTRQGYQVVVTGTVNEEPEARPLLRT
CCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
PGVTSLIGRTDLATLVAVLQQAKLVIGNDSGVTHLAAVLGRPTVAIFGPSNPDAWRPFEA
CCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCC
EIVTPETLTTTRASVLALTAALPCAPCCYVGFRVGRPQGCSTRTCLIDLHPDVVLRAALQ
EEECCCHHHHHHHHHHHHHHHCCCCHHHHHCEECCCCCCCCCCEEEEECCHHHHHHHHHH
LLEGDRDSSW
HHCCCCCCCC
>Mature Secondary Structure
MRIGRFERMLLIKIADLGDAAITLPAIHSLRKAYPQASLHVVSSPLGTELYRLSPDIDRC
CCCCHHHHEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCHHEECCCCCHHE
FVLEKSRLKRPAGALALARLLWTLRRQQYDAVALFHHLTTNAGRSLYRLLLAATASPVRA
EEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
GLDNGLGTFLTHRVPDRGFGACPEWDYARAVVEALGASPLLERPRLRVPDAAFRRAQELV
HHCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCCHHCCCCCCCCHHHHHHHHHHH
GDARDGLVVIHPGVGPFAPARRWPIESFTTVARELTRQGYQVVVTGTVNEEPEARPLLRT
CCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCC
PGVTSLIGRTDLATLVAVLQQAKLVIGNDSGVTHLAAVLGRPTVAIFGPSNPDAWRPFEA
CCHHHHHHHHHHHHHHHHHHHCCEEEECCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCC
EIVTPETLTTTRASVLALTAALPCAPCCYVGFRVGRPQGCSTRTCLIDLHPDVVLRAALQ
EEECCCHHHHHHHHHHHHHHHCCCCHHHHHCEECCCCCCCCCCEEEEECCHHHHHHHHHH
LLEGDRDSSW
HHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA