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The map label for this gene is gyrA [H]

Identifier: 221632669

GI number: 221632669

Start: 646175

End: 648685

Strand: Reverse

Name: gyrA [H]

Synonym: trd_0652

Alternate gene names: 221632669

Gene position: 648685-646175 (Counterclockwise)

Preceding gene: 221632670

Following gene: 221632668

Centisome position: 32.39

GC content: 60.97

Gene sequence:

>2511_bases
ATGGATGGCGGAGCGATGAGCGAGCAACAAATCGGACGCGTCCGTGTCATCAGCATCGAAGAGGAGATGAAGCGCTCGTA
TCTCGATTACGCGATGAGCGTGATCGTGCAACGAGCGTTGCCAGACGTGCGCGACGGCCTCAAACCAGTCCAGCGGCGCA
TCTTGTACGGCATGTACGAAATGGGCCTGCGCCCAAATGCCAAGTACCGTAAGAGCGCCGGTATCGTCGGTGAGGTCCTG
AAGTCGTATCACCCCCACGGGGACAGTGCGGTCTACGACGCCCTGGTTCGCATGGTCCAGCCATTCACCATGCGGTACCC
CTTGATCGACGGGCAGGGTAATTTCGGCTCCGTCGACGGCGATAGTGCCGCCGCGATGCGCTACACTGAGGCGCGCCTCG
CTCCGATCGCTGAAGAACTGCTCGCGGACATCGACAAGCAAACGGTCGACTTCGTTCCCAACTATGACGATAGCACGCGC
GAACCCTCGGTCCTCCCAGCCCGTCTACCGAACCTTCTCGTCAACGGGGCAAGCGGCATCGCGGTCGGTATGGCCACCAA
CATTCCCCCACACAATCTCGGCGAGGTCGTCGATGCACTGGTCTACCTCATCGATAACCCTGAAGCGACCGTCGAGGAAC
TGGTCGAACGATTGCCCGGTCCGGACTTTCCGACTGGAGGAATCATTCTGGGGCGAGAAGGAATTCTGGCCGCCTACGCC
ACCGGTCGCGGACGCATCGTCGTCCGAGCACGCGCTCACATCGAGGAGACTGGGCGGGGTCGGACCAGCATCATCGTGAC
CGAGCTACCCTATCAGGTGAACAAGGCTGCGCTCATCGAGCGCATTGCGGAACTCGTCAAGAGCGGGCGCCTGGAGGGAA
TTCATGATCTGCGCGATGAATCCGACCGCGACGGTATGCGCATCGTCATCGAACTCAAGCGTGACGCACAACCCAAGACT
GTGCTCAACAATCTGTTCAAGCATACGCAACTGCAAACCACGTTCGGCGTCAACATGCTCGCACTGGTTGATGGAGTTTT
ACCGCGTGTCCTGACCCTCAAGCGTCTGCTTCAGCTGTACTTGGAACATCGCCAACAGGTGATCCGGCGGCGTACCGAGT
TCGACCTTCGTCAAGCCGAGCGGCGAGCACACGTCTTGGAAGGTCTCAAGATCGCACTGGATCACCTCGACGAGGTCATC
AGCACGATCCGGAACGCCCGCTCGGCTGAGGATGCCCGACAGCAGTTGATGGCACGCTTCAACTTGACCGAAATCCAGGC
GAATGCCATCCTCGATATGCAACTGCGTCGCCTGGCAGCGCTCGAGCGACAGAAGATCGAGGACGAGTATCGGGAACTTC
TCGCGCGGATCGCCGAACTGCGTGCGCTTCTCGCCGACGCGGCGAAGATCTTGGCCGTCGTGCGGAAAGAGCTCCTCGAG
CTCAAGGAACGATACGGCGATCCCCGGCGAACTCAGATTCAGGAAGCCAGTAGCGAGATCAGCGACGAAGACTTGGTTCC
GCGTGTCGATGTCATCGTGACGCTGACCTCGCGAGGTTATGTGAAGCGGAGCACGAACGGACATTTCCGTGTCCAACATC
GTGGTGGCCGGGGCGTGAATGGAATGGCCGTCCGGGACGAAGACTCGATCCAGCACATCGTGCTCGCTAGTACGCATGAT
TCGCTCCTCTTTTTCACCAACCGTGGTCGCGTCTTCCAGCTGCCAACCTACGAACTTCCCGATGTGGGGCGCAACGCGCG
GGGGCTCCCGATCGTCAACTTCCTCAATCTTCAGCCAGGTGAAGAAGTCACGACACTCCTTCCCGTACGGGACTTCTCGA
CAGCCACGTTTCTGTTCTTCTGCACGCGTCAAGGCCGGGTGAAGCGTGTGCGGCTCGAGGAGTTCTCCAACGTTCGCGCG
AGCGGCCTCATCGCCATGTCGTTGGACGCGGGAGACGAACTGGCCTGGGTACGCCCCACGAGCGGCTCGGACGAGGTCAT
TCTGGTCACCGCTCATGGCCAGGCGATCCGCTTCCCAGAGGACGACGTGCGACCGATGGGCCGCCAGGCTGGCGGAGTGA
TCGGTGTTCGTTTGGACGAGGGAGACGCCGTCATCGCGGCAGAAGTCGTTCGCCCAGAAGGCGACCTTTTGCTGGTCTCC
CAGTACGGCTACGGTAAGCGCACTTCTCTCGACGAATTCCGAACCCAAGGACGCGGTGGATCCGGTGTCATCGCCATGAA
GGTCACGCCGCGCACCGGCCACGTCGCTGCCGCGACGGTCGCGCGTGAGGAAGACACCGTTGTTCTGGTCAGTCGGCGCG
GACGCATGATCCTCGTGCCGGTTGCACAAATCCCACGCCAAGGTCGGCACACGCAAGGTGTGAGCGTTATGCGACTCCAG
GAAGGGGACGAGGTTGCTTCAGTCGCGGTCGGTCTCTGGGGCGAAGCCCTCGCCCCGTCGCGATCGACCCAGGAGGTACT
CAGCGGTGACGGTGTTGAGGGAGCGAACTAG

Upstream 100 bases:

>100_bases
ACACTCGTTTCTCACAGTCAAATGCTCAGCGCGGGCGGACGCCTGGACCTATCAGGAAGTAGGGTTACGACTTTGGTAAA
ATCCAGCGGGTGGGTCAGCG

Downstream 100 bases:

>100_bases
CCAGCGACGAACCTGCGGGAAGAAGAGCGCCACCGCGATCAGCGCCTGGCCACTCCACAAGAGGACACCGAGCGGCTGAT
TCTGCAGCAGCCGTTCGAGT

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 836; Mature: 836

Protein sequence:

>836_residues
MDGGAMSEQQIGRVRVISIEEEMKRSYLDYAMSVIVQRALPDVRDGLKPVQRRILYGMYEMGLRPNAKYRKSAGIVGEVL
KSYHPHGDSAVYDALVRMVQPFTMRYPLIDGQGNFGSVDGDSAAAMRYTEARLAPIAEELLADIDKQTVDFVPNYDDSTR
EPSVLPARLPNLLVNGASGIAVGMATNIPPHNLGEVVDALVYLIDNPEATVEELVERLPGPDFPTGGIILGREGILAAYA
TGRGRIVVRARAHIEETGRGRTSIIVTELPYQVNKAALIERIAELVKSGRLEGIHDLRDESDRDGMRIVIELKRDAQPKT
VLNNLFKHTQLQTTFGVNMLALVDGVLPRVLTLKRLLQLYLEHRQQVIRRRTEFDLRQAERRAHVLEGLKIALDHLDEVI
STIRNARSAEDARQQLMARFNLTEIQANAILDMQLRRLAALERQKIEDEYRELLARIAELRALLADAAKILAVVRKELLE
LKERYGDPRRTQIQEASSEISDEDLVPRVDVIVTLTSRGYVKRSTNGHFRVQHRGGRGVNGMAVRDEDSIQHIVLASTHD
SLLFFTNRGRVFQLPTYELPDVGRNARGLPIVNFLNLQPGEEVTTLLPVRDFSTATFLFFCTRQGRVKRVRLEEFSNVRA
SGLIAMSLDAGDELAWVRPTSGSDEVILVTAHGQAIRFPEDDVRPMGRQAGGVIGVRLDEGDAVIAAEVVRPEGDLLLVS
QYGYGKRTSLDEFRTQGRGGSGVIAMKVTPRTGHVAAATVAREEDTVVLVSRRGRMILVPVAQIPRQGRHTQGVSVMRLQ
EGDEVASVAVGLWGEALAPSRSTQEVLSGDGVEGAN

Sequences:

>Translated_836_residues
MDGGAMSEQQIGRVRVISIEEEMKRSYLDYAMSVIVQRALPDVRDGLKPVQRRILYGMYEMGLRPNAKYRKSAGIVGEVL
KSYHPHGDSAVYDALVRMVQPFTMRYPLIDGQGNFGSVDGDSAAAMRYTEARLAPIAEELLADIDKQTVDFVPNYDDSTR
EPSVLPARLPNLLVNGASGIAVGMATNIPPHNLGEVVDALVYLIDNPEATVEELVERLPGPDFPTGGIILGREGILAAYA
TGRGRIVVRARAHIEETGRGRTSIIVTELPYQVNKAALIERIAELVKSGRLEGIHDLRDESDRDGMRIVIELKRDAQPKT
VLNNLFKHTQLQTTFGVNMLALVDGVLPRVLTLKRLLQLYLEHRQQVIRRRTEFDLRQAERRAHVLEGLKIALDHLDEVI
STIRNARSAEDARQQLMARFNLTEIQANAILDMQLRRLAALERQKIEDEYRELLARIAELRALLADAAKILAVVRKELLE
LKERYGDPRRTQIQEASSEISDEDLVPRVDVIVTLTSRGYVKRSTNGHFRVQHRGGRGVNGMAVRDEDSIQHIVLASTHD
SLLFFTNRGRVFQLPTYELPDVGRNARGLPIVNFLNLQPGEEVTTLLPVRDFSTATFLFFCTRQGRVKRVRLEEFSNVRA
SGLIAMSLDAGDELAWVRPTSGSDEVILVTAHGQAIRFPEDDVRPMGRQAGGVIGVRLDEGDAVIAAEVVRPEGDLLLVS
QYGYGKRTSLDEFRTQGRGGSGVIAMKVTPRTGHVAAATVAREEDTVVLVSRRGRMILVPVAQIPRQGRHTQGVSVMRLQ
EGDEVASVAVGLWGEALAPSRSTQEVLSGDGVEGAN
>Mature_836_residues
MDGGAMSEQQIGRVRVISIEEEMKRSYLDYAMSVIVQRALPDVRDGLKPVQRRILYGMYEMGLRPNAKYRKSAGIVGEVL
KSYHPHGDSAVYDALVRMVQPFTMRYPLIDGQGNFGSVDGDSAAAMRYTEARLAPIAEELLADIDKQTVDFVPNYDDSTR
EPSVLPARLPNLLVNGASGIAVGMATNIPPHNLGEVVDALVYLIDNPEATVEELVERLPGPDFPTGGIILGREGILAAYA
TGRGRIVVRARAHIEETGRGRTSIIVTELPYQVNKAALIERIAELVKSGRLEGIHDLRDESDRDGMRIVIELKRDAQPKT
VLNNLFKHTQLQTTFGVNMLALVDGVLPRVLTLKRLLQLYLEHRQQVIRRRTEFDLRQAERRAHVLEGLKIALDHLDEVI
STIRNARSAEDARQQLMARFNLTEIQANAILDMQLRRLAALERQKIEDEYRELLARIAELRALLADAAKILAVVRKELLE
LKERYGDPRRTQIQEASSEISDEDLVPRVDVIVTLTSRGYVKRSTNGHFRVQHRGGRGVNGMAVRDEDSIQHIVLASTHD
SLLFFTNRGRVFQLPTYELPDVGRNARGLPIVNFLNLQPGEEVTTLLPVRDFSTATFLFFCTRQGRVKRVRLEEFSNVRA
SGLIAMSLDAGDELAWVRPTSGSDEVILVTAHGQAIRFPEDDVRPMGRQAGGVIGVRLDEGDAVIAAEVVRPEGDLLLVS
QYGYGKRTSLDEFRTQGRGGSGVIAMKVTPRTGHVAAATVAREEDTVVLVSRRGRMILVPVAQIPRQGRHTQGVSVMRLQ
EGDEVASVAVGLWGEALAPSRSTQEVLSGDGVEGAN

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=352, Percent_Identity=25.2840909090909, Blast_Score=100, Evalue=6e-21,
Organism=Homo sapiens, GI19913406, Length=360, Percent_Identity=26.1111111111111, Blast_Score=100, Evalue=8e-21,
Organism=Escherichia coli, GI1788562, Length=853, Percent_Identity=49.3552168815944, Blast_Score=816, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=695, Percent_Identity=39.136690647482, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI212645845, Length=498, Percent_Identity=25.1004016064257, Blast_Score=94, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17535065, Length=356, Percent_Identity=25, Blast_Score=90, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI212645657, Length=354, Percent_Identity=24.5762711864407, Blast_Score=82, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115534553, Length=169, Percent_Identity=29.585798816568, Blast_Score=75, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=382, Percent_Identity=26.4397905759162, Blast_Score=96, Evalue=2e-20,
Organism=Drosophila melanogaster, GI17136538, Length=353, Percent_Identity=25.7790368271955, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 92612; Mature: 92612

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGGAMSEQQIGRVRVISIEEEMKRSYLDYAMSVIVQRALPDVRDGLKPVQRRILYGMYE
CCCCCCCHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
MGLRPNAKYRKSAGIVGEVLKSYHPHGDSAVYDALVRMVQPFTMRYPLIDGQGNFGSVDG
HCCCCCCCHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHHEECCEECCCCCCCCCCC
DSAAAMRYTEARLAPIAEELLADIDKQTVDFVPNYDDSTREPSVLPARLPNLLVNGASGI
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCHHHCCHHHCCCCCCE
AVGMATNIPPHNLGEVVDALVYLIDNPEATVEELVERLPGPDFPTGGIILGREGILAAYA
EEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEE
TGRGRIVVRARAHIEETGRGRTSIIVTELPYQVNKAALIERIAELVKSGRLEGIHDLRDE
CCCCEEEEEEEHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCC
SDRDGMRIVIELKRDAQPKTVLNNLFKHTQLQTTFGVNMLALVDGVLPRVLTLKRLLQLY
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LEHRQQVIRRRTEFDLRQAERRAHVLEGLKIALDHLDEVISTIRNARSAEDARQQLMARF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
NLTEIQANAILDMQLRRLAALERQKIEDEYRELLARIAELRALLADAAKILAVVRKELLE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKERYGDPRRTQIQEASSEISDEDLVPRVDVIVTLTSRGYVKRSTNGHFRVQHRGGRGVN
HHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEECCCCEEEEEECCCCCCC
GMAVRDEDSIQHIVLASTHDSLLFFTNRGRVFQLPTYELPDVGRNARGLPIVNFLNLQPG
CCEECCCCCCCEEEEEECCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCC
EEVTTLLPVRDFSTATFLFFCTRQGRVKRVRLEEFSNVRASGLIAMSLDAGDELAWVRPT
CCCEEEEEECCCCCEEEEEEECCCCCEEEEEHHHHCCCCCCCEEEEEECCCCCEEEEECC
SGSDEVILVTAHGQAIRFPEDDVRPMGRQAGGVIGVRLDEGDAVIAAEVVRPEGDLLLVS
CCCCCEEEEEECCCEEECCCHHCCCCHHHCCCEEEEEECCCCEEEEEEEECCCCCEEEEE
QYGYGKRTSLDEFRTQGRGGSGVIAMKVTPRTGHVAAATVAREEDTVVLVSRRGRMILVP
ECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCEEEEE
VAQIPRQGRHTQGVSVMRLQEGDEVASVAVGLWGEALAPSRSTQEVLSGDGVEGAN
HHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCC
>Mature Secondary Structure
MDGGAMSEQQIGRVRVISIEEEMKRSYLDYAMSVIVQRALPDVRDGLKPVQRRILYGMYE
CCCCCCCHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
MGLRPNAKYRKSAGIVGEVLKSYHPHGDSAVYDALVRMVQPFTMRYPLIDGQGNFGSVDG
HCCCCCCCHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHCCHHEECCEECCCCCCCCCCC
DSAAAMRYTEARLAPIAEELLADIDKQTVDFVPNYDDSTREPSVLPARLPNLLVNGASGI
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCHHHCCHHHCCCCCCE
AVGMATNIPPHNLGEVVDALVYLIDNPEATVEELVERLPGPDFPTGGIILGREGILAAYA
EEEEECCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCEEEEEE
TGRGRIVVRARAHIEETGRGRTSIIVTELPYQVNKAALIERIAELVKSGRLEGIHDLRDE
CCCCEEEEEEEHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCHHHHCCC
SDRDGMRIVIELKRDAQPKTVLNNLFKHTQLQTTFGVNMLALVDGVLPRVLTLKRLLQLY
CCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
LEHRQQVIRRRTEFDLRQAERRAHVLEGLKIALDHLDEVISTIRNARSAEDARQQLMARF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHC
NLTEIQANAILDMQLRRLAALERQKIEDEYRELLARIAELRALLADAAKILAVVRKELLE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKERYGDPRRTQIQEASSEISDEDLVPRVDVIVTLTSRGYVKRSTNGHFRVQHRGGRGVN
HHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCEEEECCCCEEEEEECCCCCCC
GMAVRDEDSIQHIVLASTHDSLLFFTNRGRVFQLPTYELPDVGRNARGLPIVNFLNLQPG
CCEECCCCCCCEEEEEECCCCEEEEECCCCEEECCCCCCCCCCCCCCCCCEEEEECCCCC
EEVTTLLPVRDFSTATFLFFCTRQGRVKRVRLEEFSNVRASGLIAMSLDAGDELAWVRPT
CCCEEEEEECCCCCEEEEEEECCCCCEEEEEHHHHCCCCCCCEEEEEECCCCCEEEEECC
SGSDEVILVTAHGQAIRFPEDDVRPMGRQAGGVIGVRLDEGDAVIAAEVVRPEGDLLLVS
CCCCCEEEEEECCCEEECCCHHCCCCHHHCCCEEEEEECCCCEEEEEEEECCCCCEEEEE
QYGYGKRTSLDEFRTQGRGGSGVIAMKVTPRTGHVAAATVAREEDTVVLVSRRGRMILVP
ECCCCCCCCHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEECCCCEEEEE
VAQIPRQGRHTQGVSVMRLQEGDEVASVAVGLWGEALAPSRSTQEVLSGDGVEGAN
HHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2987847; 7584024; 9384377 [H]