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The map label for this gene is polX [H]

Identifier: 221632593

GI number: 221632593

Start: 564693

End: 566438

Strand: Reverse

Name: polX [H]

Synonym: trd_0567

Alternate gene names: 221632593

Gene position: 566438-564693 (Counterclockwise)

Preceding gene: 221632594

Following gene: 221632588

Centisome position: 28.28

GC content: 61.45

Gene sequence:

>1746_bases
ATGCGACGGAACGAGGAGGTTGCTCTCCTGCTCGACAACATCGCTGAACTTCTGATGCTCAAGAACGAGAACCCCTATCG
GATCCGCGCCTACACGACGGCCGCACAGAACATCCGCGCGCTCGATGAGGACATCGAGGAACTCGCCCGGCAAGGAAAAC
TGGACGAAATCCCTGGCGTCGGCAAGGCGATTGCCGCCAAGATCGAAGAGTACCTGCGAACTGGTCGACTGGAATACTAT
GAACAGCTCAAGCGCGAGGTACCTTTGCAGGCAGTTGATCTGCTCGAAGTACCAGGGATCGGCCCCGCTCGAGCACACCT
TCTCTACGAACAACTCGGAATCACGACCATCCAGGACCTGCTCCAGGCAGCACAGGAGCACAAGCTGCGCAAGCTCCCCG
GCTTCGGAGAGAAACTCGAGGAGCGGATCGCCCGCGAAGCTGCACGCCTCATGCAACGGAGCAAGCGTCTCCTCCTCGGC
ATCGCGCTGCCGGTAGCCGAGGAGGTGGTGGCAGCCCTGCGACCGCATGCAGCGATCCGGGCGATCGATCCGGCCGGGAG
TCTCCGCCGCATGAAAGAGACGATCGGAGACATCGACATCCTCGTCGCCAGCGACGATCCTCCCGCAGTCGTCGATGCCT
TCGTTCATCTGCCATTCGTCAAAGAAGTCCTCGCTGTTGGACCGACGCGCCCCTCCATTCTCACCCGCGAAGACCTCCAA
GTCGATCTGCGCGTCGTGCGACCGCACGAGTACGGCTCGGCGCTTCTGTATTTCACCGGATCGAAGGAACACAACATCGC
CTTACGCTCCTTCGTCCAAGAGCGCGGCTGGAAACTCTCCGAGTACGGCCTCTTTGACGAAACCGGCAAGCTTTTGGCGA
GCGAAACGGAACATGAGATCTATGCGCTTTTGGGAATGGATTGGATTCCACCCGAGCTGCGGGAGAACCGTGGTGAGATC
GAGGCGGCCCGCGAGCATCGCTTGCCCATCCTCGTCGAGCAGCGGGATATCCGCGGCGACCTCCATGTGCACACCGATTG
GAGCGACGGACACGAGCCACTCGAGCGGATGTGCGAAGCTGCTCGCGCGCGCGGCTACGAGTACCTGGTCATTTCGGATC
ATTCCCCTTCCCTCACCGTGGCCCGCGGCTTGTCCATCGAGCGTCTGCGCGAACAACGCGAGCGGATCCGCGAACTCAAC
GAACGACTGGCACCATTTCGTATCCTGCAGGGCGCCGAAGTTAACATTTTGTCGGACGGGACACTCGACTATCCGGACGA
GATTCTCGCCGAACTCGATCTCGTGATCGTGTCCGTGCACAGCGCCTTCGATATGCCGCAGGAACGCATGACGGAACGCG
TCATCCGTGCGCTCGCCAACCCCCATGTCGACATCCTCGGTCATCCCACCGGACGCCTGCTTACCTCTCGACTCCCGTAC
GAGATCGATCTCGACGCCGTGCTGGATGCCGCTGCTCACTATGGGGTCGCGATCGAGGTCAACGGACAGCCGGATCGGCT
CGACCTCCCGGACATCTGGGTCCAGCGGGCGATCAACCGGGGAATCTTGATCGCTTGCACCAGTGATGCCCACTCGACAC
GCCAGTTGGACAATATGCGCTGGTCGGTCGCGACCGCGCGTCGCGGTTGGGCGGAGCCCAGACATGTTCTCAATACTCGG
TCGCTCGAGGAACTCCGCACCTGGCTCGCTACTCACCACCGAGGAGCTCCCGCGCACCGGACATGA

Upstream 100 bases:

>100_bases
CGGACGAAGTCCGTGCTCTCGTTCATGCGACGGTTCGACTTCTGCGCGCGTGGCTGGAGGAATCGACTGTCCAACCGACT
CGGCCGAAGGAGTGAGCGGC

Downstream 100 bases:

>100_bases
GCGGACATGCCGACTGCCAACCGAGTGCTTCCGGCGACCGACCGACCGCGCGACCGAACTCGGCGCTGCTCGGTCTGTGC
CGAGGCATACCTGCGTCCAT

Product: PHP domain-containing protein

Products: NA

Alternate protein names: DNA polymerase type-X; 3'-5' exodeoxyribonuclease; 3'-5' exonuclease [H]

Number of amino acids: Translated: 581; Mature: 581

Protein sequence:

>581_residues
MRRNEEVALLLDNIAELLMLKNENPYRIRAYTTAAQNIRALDEDIEELARQGKLDEIPGVGKAIAAKIEEYLRTGRLEYY
EQLKREVPLQAVDLLEVPGIGPARAHLLYEQLGITTIQDLLQAAQEHKLRKLPGFGEKLEERIAREAARLMQRSKRLLLG
IALPVAEEVVAALRPHAAIRAIDPAGSLRRMKETIGDIDILVASDDPPAVVDAFVHLPFVKEVLAVGPTRPSILTREDLQ
VDLRVVRPHEYGSALLYFTGSKEHNIALRSFVQERGWKLSEYGLFDETGKLLASETEHEIYALLGMDWIPPELRENRGEI
EAAREHRLPILVEQRDIRGDLHVHTDWSDGHEPLERMCEAARARGYEYLVISDHSPSLTVARGLSIERLREQRERIRELN
ERLAPFRILQGAEVNILSDGTLDYPDEILAELDLVIVSVHSAFDMPQERMTERVIRALANPHVDILGHPTGRLLTSRLPY
EIDLDAVLDAAAHYGVAIEVNGQPDRLDLPDIWVQRAINRGILIACTSDAHSTRQLDNMRWSVATARRGWAEPRHVLNTR
SLEELRTWLATHHRGAPAHRT

Sequences:

>Translated_581_residues
MRRNEEVALLLDNIAELLMLKNENPYRIRAYTTAAQNIRALDEDIEELARQGKLDEIPGVGKAIAAKIEEYLRTGRLEYY
EQLKREVPLQAVDLLEVPGIGPARAHLLYEQLGITTIQDLLQAAQEHKLRKLPGFGEKLEERIAREAARLMQRSKRLLLG
IALPVAEEVVAALRPHAAIRAIDPAGSLRRMKETIGDIDILVASDDPPAVVDAFVHLPFVKEVLAVGPTRPSILTREDLQ
VDLRVVRPHEYGSALLYFTGSKEHNIALRSFVQERGWKLSEYGLFDETGKLLASETEHEIYALLGMDWIPPELRENRGEI
EAAREHRLPILVEQRDIRGDLHVHTDWSDGHEPLERMCEAARARGYEYLVISDHSPSLTVARGLSIERLREQRERIRELN
ERLAPFRILQGAEVNILSDGTLDYPDEILAELDLVIVSVHSAFDMPQERMTERVIRALANPHVDILGHPTGRLLTSRLPY
EIDLDAVLDAAAHYGVAIEVNGQPDRLDLPDIWVQRAINRGILIACTSDAHSTRQLDNMRWSVATARRGWAEPRHVLNTR
SLEELRTWLATHHRGAPAHRT
>Mature_581_residues
MRRNEEVALLLDNIAELLMLKNENPYRIRAYTTAAQNIRALDEDIEELARQGKLDEIPGVGKAIAAKIEEYLRTGRLEYY
EQLKREVPLQAVDLLEVPGIGPARAHLLYEQLGITTIQDLLQAAQEHKLRKLPGFGEKLEERIAREAARLMQRSKRLLLG
IALPVAEEVVAALRPHAAIRAIDPAGSLRRMKETIGDIDILVASDDPPAVVDAFVHLPFVKEVLAVGPTRPSILTREDLQ
VDLRVVRPHEYGSALLYFTGSKEHNIALRSFVQERGWKLSEYGLFDETGKLLASETEHEIYALLGMDWIPPELRENRGEI
EAAREHRLPILVEQRDIRGDLHVHTDWSDGHEPLERMCEAARARGYEYLVISDHSPSLTVARGLSIERLREQRERIRELN
ERLAPFRILQGAEVNILSDGTLDYPDEILAELDLVIVSVHSAFDMPQERMTERVIRALANPHVDILGHPTGRLLTSRLPY
EIDLDAVLDAAAHYGVAIEVNGQPDRLDLPDIWVQRAINRGILIACTSDAHSTRQLDNMRWSVATARRGWAEPRHVLNTR
SLEELRTWLATHHRGAPAHRT

Specific function: Strictly DNA-template-directed DNA polymerase, preferentially acting on DNA structures containing gaps from one to a few nucleotides and bearing a phosphate group at the 5' end of the downstream DNA. The fact that PolX is able to conduct filling of a sing

COG id: COG1796

COG function: function code L; DNA polymerase IV (family X)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PHP family [H]

Homologues:

Organism=Homo sapiens, GI4505931, Length=341, Percent_Identity=27.5659824046921, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI7019491, Length=315, Percent_Identity=25.7142857142857, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI291463287, Length=315, Percent_Identity=25.7142857142857, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI291463291, Length=293, Percent_Identity=26.2798634812287, Blast_Score=85, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002054
- InterPro:   IPR010996
- InterPro:   IPR003583
- InterPro:   IPR004013
- InterPro:   IPR003141
- InterPro:   IPR016195
- InterPro:   IPR022311 [H]

Pfam domain/function: PF02811 PHP [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 65800; Mature: 65800

Theoretical pI: Translated: 5.81; Mature: 5.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRNEEVALLLDNIAELLMLKNENPYRIRAYTTAAQNIRALDEDIEELARQGKLDEIPGV
CCCCCHHHHHHHHHHHHHEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GKAIAAKIEEYLRTGRLEYYEQLKREVPLQAVDLLEVPGIGPARAHLLYEQLGITTIQDL
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHH
LQAAQEHKLRKLPGFGEKLEERIAREAARLMQRSKRLLLGIALPVAEEVVAALRPHAAIR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEE
AIDPAGSLRRMKETIGDIDILVASDDPPAVVDAFVHLPFVKEVLAVGPTRPSILTREDLQ
EECCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCE
VDLRVVRPHEYGSALLYFTGSKEHNIALRSFVQERGWKLSEYGLFDETGKLLASETEHEI
EEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEHHCCCCCHHHHHHHCCCHHHH
YALLGMDWIPPELRENRGEIEAAREHRLPILVEQRDIRGDLHVHTDWSDGHEPLERMCEA
HHHHCCCCCCHHHHCCCCCHHHHHHCCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHH
ARARGYEYLVISDHSPSLTVARGLSIERLREQRERIRELNERLAPFRILQGAEVNILSDG
HHHCCCEEEEEECCCCCEEHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCC
TLDYPDEILAELDLVIVSVHSAFDMPQERMTERVIRALANPHVDILGHPTGRLLTSRLPY
CCCCHHHHHHHHHHHEEEHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCC
EIDLDAVLDAAAHYGVAIEVNGQPDRLDLPDIWVQRAINRGILIACTSDAHSTRQLDNMR
EECHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHCCH
WSVATARRGWAEPRHVLNTRSLEELRTWLATHHRGAPAHRT
HHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRRNEEVALLLDNIAELLMLKNENPYRIRAYTTAAQNIRALDEDIEELARQGKLDEIPGV
CCCCCHHHHHHHHHHHHHEECCCCCEEEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCC
GKAIAAKIEEYLRTGRLEYYEQLKREVPLQAVDLLEVPGIGPARAHLLYEQLGITTIQDL
HHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHH
LQAAQEHKLRKLPGFGEKLEERIAREAARLMQRSKRLLLGIALPVAEEVVAALRPHAAIR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEE
AIDPAGSLRRMKETIGDIDILVASDDPPAVVDAFVHLPFVKEVLAVGPTRPSILTREDLQ
EECCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEECCCCE
VDLRVVRPHEYGSALLYFTGSKEHNIALRSFVQERGWKLSEYGLFDETGKLLASETEHEI
EEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHCCEEHHCCCCCHHHHHHHCCCHHHH
YALLGMDWIPPELRENRGEIEAAREHRLPILVEQRDIRGDLHVHTDWSDGHEPLERMCEA
HHHHCCCCCCHHHHCCCCCHHHHHHCCCCEEEECCCCCCCEEEECCCCCCHHHHHHHHHH
ARARGYEYLVISDHSPSLTVARGLSIERLREQRERIRELNERLAPFRILQGAEVNILSDG
HHHCCCEEEEEECCCCCEEHHCCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCC
TLDYPDEILAELDLVIVSVHSAFDMPQERMTERVIRALANPHVDILGHPTGRLLTSRLPY
CCCCHHHHHHHHHHHEEEHHHHHCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCC
EIDLDAVLDAAAHYGVAIEVNGQPDRLDLPDIWVQRAINRGILIACTSDAHSTRQLDNMR
EECHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHCCCEEEEECCCCHHHHHHHCCH
WSVATARRGWAEPRHVLNTRSLEELRTWLATHHRGAPAHRT
HHHHHHHCCCCCHHHHHHHCCHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]