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The map label for this gene is sucB [H]

Identifier: 221632586

GI number: 221632586

Start: 554704

End: 556023

Strand: Reverse

Name: sucB [H]

Synonym: trd_0561

Alternate gene names: 221632586

Gene position: 556023-554704 (Counterclockwise)

Preceding gene: 221632587

Following gene: 221632585

Centisome position: 27.76

GC content: 63.03

Gene sequence:

>1320_bases
ATGGCTATCGAGGTTCGTGTGCCGCAGATGGGCGAGTCGATCGTCGAGGCAGTCATCGGCGCCTGGCGGAAACGCGAAGG
TGATCCGGTCAACCCAGGCGACGTGCTCGTCGAGTTGGAAACCGAAAAAGTCAACGTCGAAGTGACAGCGGATCGAGCTG
GTGTTCTCCAGCACATCCTCAAGCGCGAGGGAGAAACGGTGACCGTCGGCGAGGTCATCGCACTGATCGAGGAGACCGCG
CAGGTGACTGCTCCCGTTCAGGAAACCCCTGCCGAAGCGGGCAGAGCACGAGAGCCAGCTCCCTTGCCCGAGGTCACGAC
AGCCGCAGCGTCGCAACCGGCGCAACCCGCCGGCGGACCAGCGGTTAGGGCGACGCCAGCTGTCAGGCGACTGGCCGAGG
AATACGGTATCGACCTCGCTGAGGTACCTGCTTCCGGCGAAGGTGGTCGGGTCACTCGGGAGGACGTGCTCCGTTACGTT
GCTCAGCGTGGAGCAGCCGCCCGACCGGCTGACGGTGCGGCTCCCCAGCCAGTCGCAGCGGCCCCACCGCCAGCCGCACC
ACCGGCACCAGGTGTCACACCACCGGAGGCACCGGTTGTCTTGCCCTTCTCTGCTGCCGGACGCCGCGAAGAACGCATTC
GGATGAGCCAGCGACGCAAGACGATCGCCCGGCGGTTGGTCGAGGCCCAGCACACCGCAGCGATGCTGACGACCTTCAAC
GAGATCGACATGAGTGCCGTGGTCGCCCTTCGCAACCGGTGGCGCCAGCAGTTCCGGGAACGCTACGGCGTGAGCCTCGG
CTTCATGTCATTCTTCACCAAAGCTGTCGTCGGTGCGTTGAAAGCTTTCCCCTTGTTGAACGCCGAGATCCAGGGTGACG
AGATCGTCGTCAAATATTACTACGACATCGGAATCGCCGTCGCGACCGATGAAGGACTCGTCGTGCCCGTCGTCCGCGAC
GCGGACCGTAAGTCGTTCGCGGAGATCGAGCGCGAGATCGAAGAACTCGCCCGCAAGGCACGGGAACGACGACTGACGCT
CGAGGAACTCAGCGGTGGAACCTTTACGATCACCAATGGCGGCGTTTTCGGCTCTCTCCTCTCGACCCCGATTCTCAATC
CCCCTCAGGTCGGCATCCTCGGGATGCACAAGATCGAGGAACGACCGGTCGTGGTCAACGGCGAGATCGTCATTCGCCCG
ATGATGTACGTTGCCCTCACGTATGACCATCGGATCGTGGACGGTCGGGAAGCTGTGCTTTTCCTCGTCCGCGTGAAAGA
ACTCATCGAGGACCCAGAACGCCTGCTGCTCGAAGGCTGA

Upstream 100 bases:

>100_bases
CCGAAATGCACGAGCAGGAGCAGGCACGGATCATCGCCGAAGCATTCGCTGGCGTACCTGAACCTGCTGTTCCGGCATGA
ACCGAACGCGAGGGTAGACG

Downstream 100 bases:

>100_bases
ACGCGATGAGCGTCACCGAACTGGCGTGGGCAACTACACCGAACGGCACCAAAATCGCGTACGAGCGGCGGGTCGGATCG
GCCGGCCCGCCGATCCTCGC

Product: 2-oxoglutarate dehydrogenase, E2 component, dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MAIEVRVPQMGESIVEAVIGAWRKREGDPVNPGDVLVELETEKVNVEVTADRAGVLQHILKREGETVTVGEVIALIEETA
QVTAPVQETPAEAGRAREPAPLPEVTTAAASQPAQPAGGPAVRATPAVRRLAEEYGIDLAEVPASGEGGRVTREDVLRYV
AQRGAAARPADGAAPQPVAAAPPPAAPPAPGVTPPEAPVVLPFSAAGRREERIRMSQRRKTIARRLVEAQHTAAMLTTFN
EIDMSAVVALRNRWRQQFRERYGVSLGFMSFFTKAVVGALKAFPLLNAEIQGDEIVVKYYYDIGIAVATDEGLVVPVVRD
ADRKSFAEIEREIEELARKARERRLTLEELSGGTFTITNGGVFGSLLSTPILNPPQVGILGMHKIEERPVVVNGEIVIRP
MMYVALTYDHRIVDGREAVLFLVRVKELIEDPERLLLEG

Sequences:

>Translated_439_residues
MAIEVRVPQMGESIVEAVIGAWRKREGDPVNPGDVLVELETEKVNVEVTADRAGVLQHILKREGETVTVGEVIALIEETA
QVTAPVQETPAEAGRAREPAPLPEVTTAAASQPAQPAGGPAVRATPAVRRLAEEYGIDLAEVPASGEGGRVTREDVLRYV
AQRGAAARPADGAAPQPVAAAPPPAAPPAPGVTPPEAPVVLPFSAAGRREERIRMSQRRKTIARRLVEAQHTAAMLTTFN
EIDMSAVVALRNRWRQQFRERYGVSLGFMSFFTKAVVGALKAFPLLNAEIQGDEIVVKYYYDIGIAVATDEGLVVPVVRD
ADRKSFAEIEREIEELARKARERRLTLEELSGGTFTITNGGVFGSLLSTPILNPPQVGILGMHKIEERPVVVNGEIVIRP
MMYVALTYDHRIVDGREAVLFLVRVKELIEDPERLLLEG
>Mature_438_residues
AIEVRVPQMGESIVEAVIGAWRKREGDPVNPGDVLVELETEKVNVEVTADRAGVLQHILKREGETVTVGEVIALIEETAQ
VTAPVQETPAEAGRAREPAPLPEVTTAAASQPAQPAGGPAVRATPAVRRLAEEYGIDLAEVPASGEGGRVTREDVLRYVA
QRGAAARPADGAAPQPVAAAPPPAAPPAPGVTPPEAPVVLPFSAAGRREERIRMSQRRKTIARRLVEAQHTAAMLTTFNE
IDMSAVVALRNRWRQQFRERYGVSLGFMSFFTKAVVGALKAFPLLNAEIQGDEIVVKYYYDIGIAVATDEGLVVPVVRDA
DRKSFAEIEREIEELARKARERRLTLEELSGGTFTITNGGVFGSLLSTPILNPPQVGILGMHKIEERPVVVNGEIVIRPM
MYVALTYDHRIVDGREAVLFLVRVKELIEDPERLLLEG

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=252, Percent_Identity=54.3650793650794, Blast_Score=270, Evalue=3e-72,
Organism=Homo sapiens, GI110671329, Length=443, Percent_Identity=29.3453724604966, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=30.58568329718, Blast_Score=176, Evalue=4e-44,
Organism=Homo sapiens, GI203098816, Length=464, Percent_Identity=30.6034482758621, Blast_Score=175, Evalue=9e-44,
Organism=Homo sapiens, GI31711992, Length=454, Percent_Identity=29.9559471365639, Blast_Score=165, Evalue=8e-41,
Organism=Homo sapiens, GI260898739, Length=143, Percent_Identity=38.4615384615385, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=440, Percent_Identity=44.3181818181818, Blast_Score=362, Evalue=1e-101,
Organism=Escherichia coli, GI1786305, Length=441, Percent_Identity=31.2925170068027, Blast_Score=182, Evalue=3e-47,
Organism=Caenorhabditis elegans, GI25146366, Length=457, Percent_Identity=37.636761487965, Blast_Score=279, Evalue=3e-75,
Organism=Caenorhabditis elegans, GI17537937, Length=441, Percent_Identity=28.3446712018141, Blast_Score=172, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=463, Percent_Identity=30.8855291576674, Blast_Score=169, Evalue=3e-42,
Organism=Caenorhabditis elegans, GI17538894, Length=339, Percent_Identity=27.7286135693215, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6320352, Length=438, Percent_Identity=41.324200913242, Blast_Score=321, Evalue=2e-88,
Organism=Saccharomyces cerevisiae, GI6324258, Length=460, Percent_Identity=28.4782608695652, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321632, Length=176, Percent_Identity=30.6818181818182, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI18859875, Length=443, Percent_Identity=28.6681715575621, Blast_Score=172, Evalue=6e-43,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=31.4655172413793, Blast_Score=116, Evalue=3e-26,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=31.4655172413793, Blast_Score=116, Evalue=3e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 47630; Mature: 47499

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIEVRVPQMGESIVEAVIGAWRKREGDPVNPGDVLVELETEKVNVEVTADRAGVLQHIL
CEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEEECCHHHHHHHHH
KREGETVTVGEVIALIEETAQVTAPVQETPAEAGRAREPAPLPEVTTAAASQPAQPAGGP
HCCCCEEEHHHHHHHHHHHHHHCCCHHHCCHHHCCCCCCCCCCCHHHHHCCCCCCCCCCC
AVRATPAVRRLAEEYGIDLAEVPASGEGGRVTREDVLRYVAQRGAAARPADGAAPQPVAA
CCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
APPPAAPPAPGVTPPEAPVVLPFSAAGRREERIRMSQRRKTIARRLVEAQHTAAMLTTFN
CCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDMSAVVALRNRWRQQFRERYGVSLGFMSFFTKAVVGALKAFPLLNAEIQGDEIVVKYY
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
YDIGIAVATDEGLVVPVVRDADRKSFAEIEREIEELARKARERRLTLEELSGGTFTITNG
EECEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECC
GVFGSLLSTPILNPPQVGILGMHKIEERPVVVNGEIVIRPMMYVALTYDHRIVDGREAVL
CHHHHHHHCCCCCCCCCCEEEHHHHCCCCEEECCCEEEEEEEEEEEECCCEEECCHHHHH
FLVRVKELIEDPERLLLEG
HHHHHHHHHCCHHHHHCCC
>Mature Secondary Structure 
AIEVRVPQMGESIVEAVIGAWRKREGDPVNPGDVLVELETEKVNVEVTADRAGVLQHIL
EEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEEEECCHHHHHHHHH
KREGETVTVGEVIALIEETAQVTAPVQETPAEAGRAREPAPLPEVTTAAASQPAQPAGGP
HCCCCEEEHHHHHHHHHHHHHHCCCHHHCCHHHCCCCCCCCCCCHHHHHCCCCCCCCCCC
AVRATPAVRRLAEEYGIDLAEVPASGEGGRVTREDVLRYVAQRGAAARPADGAAPQPVAA
CCCCCHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCC
APPPAAPPAPGVTPPEAPVVLPFSAAGRREERIRMSQRRKTIARRLVEAQHTAAMLTTFN
CCCCCCCCCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDMSAVVALRNRWRQQFRERYGVSLGFMSFFTKAVVGALKAFPLLNAEIQGDEIVVKYY
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEECCCEEEEEEE
YDIGIAVATDEGLVVPVVRDADRKSFAEIEREIEELARKARERRLTLEELSGGTFTITNG
EECEEEEECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECC
GVFGSLLSTPILNPPQVGILGMHKIEERPVVVNGEIVIRPMMYVALTYDHRIVDGREAVL
CHHHHHHHCCCCCCCCCCEEEHHHHCCCCEEECCCEEEEEEEEEEEECCCEEECCHHHHH
FLVRVKELIEDPERLLLEG
HHHHHHHHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2500417; 9384377 [H]