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The map label for this gene is ilvB [H]

Identifier: 221632584

GI number: 221632584

Start: 552014

End: 553639

Strand: Reverse

Name: ilvB [H]

Synonym: trd_0559

Alternate gene names: 221632584

Gene position: 553639-552014 (Counterclockwise)

Preceding gene: 221632585

Following gene: 221632579

Centisome position: 27.64

GC content: 62.67

Gene sequence:

>1626_bases
ATGCCCGTGCTGACCGGAGGTCAGGCGCTGGTCGCGCAACTACTCGAGGAAGGGATCGAGATCGTCTTCGGACTTCCCGG
TGTCCAACTCGACTGGGCCTTCGATGCCCTCTACGAGGCGCGCGACCGGATCCGCGTCATCCACACACGACACGAACAAG
CCACGAGCTACATGGCCGACGGCTACGCTCGCACCACCGGCAAGATGGGCGTCTGCTTGGTCGTTCCCGGCCCAGGCGTG
CTCAACGCCATGGCTGGGCTGGCCACGGCCTACGCCTGCAATTCGCGCGTTTTCTGCATCACCGGACAAATCCCTTCGGA
CCAGATCGAAAAGGGGCGAGGGTTTCTGCACGAGATTCCTCGTCAGCTGGAGGCGCTCCGCTCGGTCACGAAATGGGCTG
CCCGAGCGATGCATCCCAGCCAAATCCCCGCACTCGCCCATGAGGCGATCGTTCAGCTCTGGAGCGGACGCCCCCGGCCC
GTTGCGCTCGAAATACCACCCGATGTGCTGCAGGCGACTGCTGAGGTGGAAACGATTGCAGCGACACGTCCTCTCCCACC
TGACGGGGATCCCGAGCTGATCGAACGGGCCGCCCAACTCCTGGGTACGGCCCAAAAACCGCTTATCTTCGCGGGTGGCG
GTATCCTCGCCGCCGAAGCCTGGGAAGAACTGCGGGCGCTGGCCGAACTCCTCGAAGCACCGGTCGTCCTCACGATGGAC
GGGAAAGGGGCCCTCGACGACCGGCATGACCTCGCCCACAACATCATCGCTGCCCGCGAACTCGTTCCGGAGTCCGATGT
CATACTCATCGTCGGCTCCCGCTTCCTCCAGCCAGCGACCTCGGATTGGGGACCTCGTCACCAAGCGGTCATTCAGATCG
ATATCGACCCGGACGAGATCGGTCGGAACTATCCCCCGACGATCGGCATCGTCGCCGACGCCAAACGAGCGCTTTCGGCA
CTCCTGGAGCGAGTTCCGCTCTATAACCAAAAACGTGACTCGCGACGGGCGGAACTGCGCGCTCTCAAGGAACGAGTCTT
CGATCTGCTCTGGGAAGTCCAACCCCAAGCATCGTTCGCGATGGCGCTCCGCGAGGCGCTCCCCGAAGAAGCCATCGTGG
TGAGCGAGAGTACCCAGGTCGGCTACTGGACGTGGAACGCTTTCCCGGTCTATCGACCACGCACCTTCGTCACGTCGGGA
TATCAGGGGACTCTGGGCTTCGGCTTTGCGACAGCTTTGGGGGCGAAAGCGGGCAACCCTGATCGGCCGGTTCTCTCGCT
GAACGGCGATGGAGGATTTCTGTACAACCTGCCTGAACTCGCTACCATGGTGCAGCATCGCCTGGACGTGATCACGGTCG
TCTTCAACGACAACGCCTTTGGGAACGTCCGCCGGATCCAGAAGGAACGATTCGGTGGGCACTACATCGCATCCGAGCTC
TACAATCCGGATTTCGTCGCGCTCGCCCACAGCTTCGGTATCGAGGGAGTGCGCGTTTCCACTCCAGAAGCCCTTCGCGA
CATCGTCGCCGATACCGTTCGGAATCCCCGACCCATCTTGATCGAGGTTCCCGTTGGCGAGATGCCGAGCATCTGGGGCA
TCGTCATGGCCGGGCGGCGCTCCTGA

Upstream 100 bases:

>100_bases
CCACGTCATCGTTTCCCGGCACCGCTGGCCAGAATGACGCGACGCAGCGCTGCACATATCGTCGTTCGGGTCGAATGCGA
CGTGCGAGAGGAGCAACGCA

Downstream 100 bases:

>100_bases
AGATCGCGGATAACTGGTCGTTTTCACTGGTGTCCGCTCGCAGGGCAGGTGACCCGTACGCGAACACGTCTCGACTGAGG
TACCGGAAGCACGTTGTCAA

Product: hypothetical protein

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 541; Mature: 540

Protein sequence:

>541_residues
MPVLTGGQALVAQLLEEGIEIVFGLPGVQLDWAFDALYEARDRIRVIHTRHEQATSYMADGYARTTGKMGVCLVVPGPGV
LNAMAGLATAYACNSRVFCITGQIPSDQIEKGRGFLHEIPRQLEALRSVTKWAARAMHPSQIPALAHEAIVQLWSGRPRP
VALEIPPDVLQATAEVETIAATRPLPPDGDPELIERAAQLLGTAQKPLIFAGGGILAAEAWEELRALAELLEAPVVLTMD
GKGALDDRHDLAHNIIAARELVPESDVILIVGSRFLQPATSDWGPRHQAVIQIDIDPDEIGRNYPPTIGIVADAKRALSA
LLERVPLYNQKRDSRRAELRALKERVFDLLWEVQPQASFAMALREALPEEAIVVSESTQVGYWTWNAFPVYRPRTFVTSG
YQGTLGFGFATALGAKAGNPDRPVLSLNGDGGFLYNLPELATMVQHRLDVITVVFNDNAFGNVRRIQKERFGGHYIASEL
YNPDFVALAHSFGIEGVRVSTPEALRDIVADTVRNPRPILIEVPVGEMPSIWGIVMAGRRS

Sequences:

>Translated_541_residues
MPVLTGGQALVAQLLEEGIEIVFGLPGVQLDWAFDALYEARDRIRVIHTRHEQATSYMADGYARTTGKMGVCLVVPGPGV
LNAMAGLATAYACNSRVFCITGQIPSDQIEKGRGFLHEIPRQLEALRSVTKWAARAMHPSQIPALAHEAIVQLWSGRPRP
VALEIPPDVLQATAEVETIAATRPLPPDGDPELIERAAQLLGTAQKPLIFAGGGILAAEAWEELRALAELLEAPVVLTMD
GKGALDDRHDLAHNIIAARELVPESDVILIVGSRFLQPATSDWGPRHQAVIQIDIDPDEIGRNYPPTIGIVADAKRALSA
LLERVPLYNQKRDSRRAELRALKERVFDLLWEVQPQASFAMALREALPEEAIVVSESTQVGYWTWNAFPVYRPRTFVTSG
YQGTLGFGFATALGAKAGNPDRPVLSLNGDGGFLYNLPELATMVQHRLDVITVVFNDNAFGNVRRIQKERFGGHYIASEL
YNPDFVALAHSFGIEGVRVSTPEALRDIVADTVRNPRPILIEVPVGEMPSIWGIVMAGRRS
>Mature_540_residues
PVLTGGQALVAQLLEEGIEIVFGLPGVQLDWAFDALYEARDRIRVIHTRHEQATSYMADGYARTTGKMGVCLVVPGPGVL
NAMAGLATAYACNSRVFCITGQIPSDQIEKGRGFLHEIPRQLEALRSVTKWAARAMHPSQIPALAHEAIVQLWSGRPRPV
ALEIPPDVLQATAEVETIAATRPLPPDGDPELIERAAQLLGTAQKPLIFAGGGILAAEAWEELRALAELLEAPVVLTMDG
KGALDDRHDLAHNIIAARELVPESDVILIVGSRFLQPATSDWGPRHQAVIQIDIDPDEIGRNYPPTIGIVADAKRALSAL
LERVPLYNQKRDSRRAELRALKERVFDLLWEVQPQASFAMALREALPEEAIVVSESTQVGYWTWNAFPVYRPRTFVTSGY
QGTLGFGFATALGAKAGNPDRPVLSLNGDGGFLYNLPELATMVQHRLDVITVVFNDNAFGNVRRIQKERFGGHYIASELY
NPDFVALAHSFGIEGVRVSTPEALRDIVADTVRNPRPILIEVPVGEMPSIWGIVMAGRRS

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=553, Percent_Identity=27.4864376130199, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI21361361, Length=578, Percent_Identity=24.5674740484429, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI87081685, Length=553, Percent_Identity=32.368896925859, Blast_Score=244, Evalue=1e-65,
Organism=Escherichia coli, GI1790104, Length=551, Percent_Identity=31.2159709618875, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI1786717, Length=560, Percent_Identity=30, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1788716, Length=540, Percent_Identity=27.4074074074074, Blast_Score=155, Evalue=7e-39,
Organism=Escherichia coli, GI1787096, Length=540, Percent_Identity=27.7777777777778, Blast_Score=145, Evalue=6e-36,
Organism=Caenorhabditis elegans, GI17531299, Length=486, Percent_Identity=29.6296296296296, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17531301, Length=487, Percent_Identity=29.5687885010267, Blast_Score=137, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI17542570, Length=571, Percent_Identity=25.569176882662, Blast_Score=123, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6323755, Length=578, Percent_Identity=28.2006920415225, Blast_Score=216, Evalue=9e-57,
Organism=Saccharomyces cerevisiae, GI6320816, Length=475, Percent_Identity=26.3157894736842, Blast_Score=111, Evalue=3e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=568, Percent_Identity=24.2957746478873, Blast_Score=97, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321524, Length=573, Percent_Identity=22.6876090750436, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6323073, Length=572, Percent_Identity=22.3776223776224, Blast_Score=87, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6320123, Length=583, Percent_Identity=21.7838765008576, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI19922626, Length=552, Percent_Identity=29.3478260869565, Blast_Score=176, Evalue=4e-44,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 59142; Mature: 59010

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVLTGGQALVAQLLEEGIEIVFGLPGVQLDWAFDALYEARDRIRVIHTRHEQATSYMAD
CCCCCCHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHEEEEEHHHHHHHHHHHC
GYARTTGKMGVCLVVPGPGVLNAMAGLATAYACNSRVFCITGQIPSDQIEKGRGFLHEIP
CHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCHHHHHH
RQLEALRSVTKWAARAMHPSQIPALAHEAIVQLWSGRPRPVALEIPPDVLQATAEVETIA
HHHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHH
ATRPLPPDGDPELIERAAQLLGTAQKPLIFAGGGILAAEAWEELRALAELLEAPVVLTMD
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEC
GKGALDDRHDLAHNIIAARELVPESDVILIVGSRFLQPATSDWGPRHQAVIQIDIDPDEI
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCHHHH
GRNYPPTIGIVADAKRALSALLERVPLYNQKRDSRRAELRALKERVFDLLWEVQPQASFA
CCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
MALREALPEEAIVVSESTQVGYWTWNAFPVYRPRTFVTSGYQGTLGFGFATALGAKAGNP
HHHHHHCCCCEEEEECCCEEEEEEECCEECCCCCEEEECCCCCCCHHHHHHHHCCCCCCC
DRPVLSLNGDGGFLYNLPELATMVQHRLDVITVVFNDNAFGNVRRIQKERFGGHYIASEL
CCCEEEECCCCCEEECHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCEEHHHHC
YNPDFVALAHSFGIEGVRVSTPEALRDIVADTVRNPRPILIEVPVGEMPSIWGIVMAGRR
CCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHEECCCC
S
C
>Mature Secondary Structure 
PVLTGGQALVAQLLEEGIEIVFGLPGVQLDWAFDALYEARDRIRVIHTRHEQATSYMAD
CCCCCHHHHHHHHHHCCCEEEECCCCCEEHHHHHHHHHHHHHEEEEEHHHHHHHHHHHC
GYARTTGKMGVCLVVPGPGVLNAMAGLATAYACNSRVFCITGQIPSDQIEKGRGFLHEIP
CHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHCCCHHHHHH
RQLEALRSVTKWAARAMHPSQIPALAHEAIVQLWSGRPRPVALEIPPDVLQATAEVETIA
HHHHHHHHHHHHHHHCCCCHHCHHHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHH
ATRPLPPDGDPELIERAAQLLGTAQKPLIFAGGGILAAEAWEELRALAELLEAPVVLTMD
CCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCEEEEEC
GKGALDDRHDLAHNIIAARELVPESDVILIVGSRFLQPATSDWGPRHQAVIQIDIDPDEI
CCCCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEEECCHHHH
GRNYPPTIGIVADAKRALSALLERVPLYNQKRDSRRAELRALKERVFDLLWEVQPQASFA
CCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHH
MALREALPEEAIVVSESTQVGYWTWNAFPVYRPRTFVTSGYQGTLGFGFATALGAKAGNP
HHHHHHCCCCEEEEECCCEEEEEEECCEECCCCCEEEECCCCCCCHHHHHHHHCCCCCCC
DRPVLSLNGDGGFLYNLPELATMVQHRLDVITVVFNDNAFGNVRRIQKERFGGHYIASEL
CCCEEEECCCCCEEECHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHCCCEEHHHHC
YNPDFVALAHSFGIEGVRVSTPEALRDIVADTVRNPRPILIEVPVGEMPSIWGIVMAGRR
CCCCHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHEECCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]