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The map label for this gene is potB [H]

Identifier: 221632547

GI number: 221632547

Start: 518911

End: 519825

Strand: Reverse

Name: potB [H]

Synonym: trd_0522

Alternate gene names: 221632547

Gene position: 519825-518911 (Counterclockwise)

Preceding gene: 221632548

Following gene: 221632546

Centisome position: 25.95

GC content: 59.45

Gene sequence:

>915_bases
ATGAGCGCCATCGACCGTGTACGAGTCCAAGAGCGTTCCCTCGGCTGGCACGAGCGCGCCAGTGAGCGGATCCGGCAAAG
CGAAAGAGTGCGCGCTCTCTTCCTCGCCGGGCCAGGCGGTTTGTGGCTCCTCCTGTTCTTCTTGTTACCGACAGCCATCG
TGTTCGCCTACAGTTTCGCTCGCCGCGGCCCCTATGGAGCGGTAGCCTGGGCTTTCACGCTGGAGAACTACGCGCGCTTG
TTCGACTGGCTGTACATTCGAGTCTTTCTCATGTCGGTTTGGCTCGCCTTGCTCACCACGGTCATTTGTCTCGTCATCGG
TTATCCCTTCGCCTTCGTCATCGCCCGGAGTCCACGCCGCTGGCGCAACGCACTCCTCGTCCTCGTCCTGATCCCGTTCT
GGACGAACTTTCTGGTGCGCACCTATGCCATCATGCTCCTCTTGCGCGCTGACGGGCTCATCAACAACGCGCTGCAGTGG
CTGGGGCTGATCGATCGTCCTCTCTCTCTGCTCTTCACCCCCTTCGCCGTCGTGCTCGGTCTCGTCTACGGCTATCTTCC
CTTCATGATCCTACCGATCTATGCATCGATCGAGAAGTTCGACTTCACGCTGGTCGAGGCTGCACAGGATCTCGGTGCGA
ATGCGTGGCACGTCTTCTGGCGCGTCCTGCTTCCTCTTACGCTTCCTGGTGTTCTCGCTGGCTCGATTCTCGTCTTCATT
CCGTCAGTCGGTGCCTTCGTGACCCCTGACTTGCTCGGGGGCGGCAAGGTAGTCATGATCGCCAATCTGATCAATCAGCA
GTTCCTGACCGTGCGACACTGGCCCTTCGGTTCAGCGATTTCGTTCGTCTTGATGGCGATCGTTCTCGCAGCGACCATGC
TCTACTTCCGAATCGGGGAGACGCGGCGGATATGA

Upstream 100 bases:

>100_bases
GGACGGGGGACTGCTCCTTCCCGTCGGGACTCCCGTATCGCTCACTTGGGCGACCGATGCGGCACGGATCCTCACCGAGT
AGCGCGAGAGGAACGCCAAG

Downstream 100 bases:

>100_bases
GTTCGATGGCAACGGCACGACCAGCGACGACGATGCGGAGCAAACGCCGTTTTTCTTTCGGGTGGGCAGTGCTCAATCTC
AATGTATTACTAACTTTCAT

Product: spermidine/putrescine transport system permease potB

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MSAIDRVRVQERSLGWHERASERIRQSERVRALFLAGPGGLWLLLFFLLPTAIVFAYSFARRGPYGAVAWAFTLENYARL
FDWLYIRVFLMSVWLALLTTVICLVIGYPFAFVIARSPRRWRNALLVLVLIPFWTNFLVRTYAIMLLLRADGLINNALQW
LGLIDRPLSLLFTPFAVVLGLVYGYLPFMILPIYASIEKFDFTLVEAAQDLGANAWHVFWRVLLPLTLPGVLAGSILVFI
PSVGAFVTPDLLGGGKVVMIANLINQQFLTVRHWPFGSAISFVLMAIVLAATMLYFRIGETRRI

Sequences:

>Translated_304_residues
MSAIDRVRVQERSLGWHERASERIRQSERVRALFLAGPGGLWLLLFFLLPTAIVFAYSFARRGPYGAVAWAFTLENYARL
FDWLYIRVFLMSVWLALLTTVICLVIGYPFAFVIARSPRRWRNALLVLVLIPFWTNFLVRTYAIMLLLRADGLINNALQW
LGLIDRPLSLLFTPFAVVLGLVYGYLPFMILPIYASIEKFDFTLVEAAQDLGANAWHVFWRVLLPLTLPGVLAGSILVFI
PSVGAFVTPDLLGGGKVVMIANLINQQFLTVRHWPFGSAISFVLMAIVLAATMLYFRIGETRRI
>Mature_303_residues
SAIDRVRVQERSLGWHERASERIRQSERVRALFLAGPGGLWLLLFFLLPTAIVFAYSFARRGPYGAVAWAFTLENYARLF
DWLYIRVFLMSVWLALLTTVICLVIGYPFAFVIARSPRRWRNALLVLVLIPFWTNFLVRTYAIMLLLRADGLINNALQWL
GLIDRPLSLLFTPFAVVLGLVYGYLPFMILPIYASIEKFDFTLVEAAQDLGANAWHVFWRVLLPLTLPGVLAGSILVFIP
SVGAFVTPDLLGGGKVVMIANLINQQFLTVRHWPFGSAISFVLMAIVLAATMLYFRIGETRRI

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=256, Percent_Identity=47.265625, Blast_Score=248, Evalue=4e-67,
Organism=Escherichia coli, GI1787080, Length=240, Percent_Identity=44.5833333333333, Blast_Score=210, Evalue=1e-55,
Organism=Escherichia coli, GI1787713, Length=243, Percent_Identity=32.9218106995885, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1786980, Length=166, Percent_Identity=28.9156626506024, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 34473; Mature: 34342

Theoretical pI: Translated: 10.66; Mature: 10.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIDRVRVQERSLGWHERASERIRQSERVRALFLAGPGGLWLLLFFLLPTAIVFAYSFA
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHH
RRGPYGAVAWAFTLENYARLFDWLYIRVFLMSVWLALLTTVICLVIGYPFAFVIARSPRR
HCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHH
WRNALLVLVLIPFWTNFLVRTYAIMLLLRADGLINNALQWLGLIDRPLSLLFTPFAVVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVYGYLPFMILPIYASIEKFDFTLVEAAQDLGANAWHVFWRVLLPLTLPGVLAGSILVFI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
PSVGAFVTPDLLGGGKVVMIANLINQQFLTVRHWPFGSAISFVLMAIVLAATMLYFRIGE
CCCCHHHCHHHCCCCHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHHHCC
TRRI
CCCC
>Mature Secondary Structure 
SAIDRVRVQERSLGWHERASERIRQSERVRALFLAGPGGLWLLLFFLLPTAIVFAYSFA
CCHHHHHHHHHCCCHHHHHHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHH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CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]