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The map label for this gene is actII-2 [H]

Identifier: 221632518

GI number: 221632518

Start: 487703

End: 489337

Strand: Reverse

Name: actII-2 [H]

Synonym: trd_0492

Alternate gene names: 221632518

Gene position: 489337-487703 (Counterclockwise)

Preceding gene: 221632519

Following gene: 221632511

Centisome position: 24.43

GC content: 64.16

Gene sequence:

>1635_bases
ATGACAGCGGCACCACGCACCCGATGGCTTGGGCTCGCGTCGCTCGCCCTCGGTCTAGCTATGATCATCGTCGATGCGAC
GATCGTCAATGTCGCGATTCCCAGTATCATCGAGGATCTCGGTCTCCAGTTAACTGATGCGGAGTGGGTGAATACGGTCT
ACTCGCTCGTCTTCGCTGCTCTACTGGTCACGGTCGGACGTCTCGGTGACTTGTACGGACGCAAGCGGATCTACCTCGGC
GGGCTAGCCCTCTTCGTCGGCGCCAGTCTCCTCTGTGCCAGTGCTCCATCCGGGACACTGCTCATCGCGGCACGTGCTCT
CCAGGGCATCGGCGCAGCCTGCATCCTGCCCTCGACCCTTTCCAGCGTCAACGCCATGTTCCGGGGACGCGAGCGAGCCC
TCGCCTTCGGCATCTGGGGATCCGTGATCGGTGGCATGGCAGCGCTCGGGCCGCTGGTCGGTGGTTGGCTGACCACCGAC
TTCTCGTGGCGCTGGGCCTTTTACATCAATTTGCCGATCGGCTTGGTCGCTGGAACCATCGCCCTCCTCACCGTCCCGGA
AACGCGTGATCCTTCAGCGGCTCCTGGCATCGATCTGATCGGCATCGTGACCAGCGCCATCGGATTCGCTGCGCTCGTCT
TCGGCTTGATCGAGGGAGAGCGCTATGGTTGGTGGCGACCGACGGACACGTTCCGCATCGGTTCGTGGGAATGGCCATTC
GCCACGCTGTCACCTGTGCCGGTGGCCCTGGCCCTCGCTGCGCTGTTCCTTCCAACGTTCGCCCTCTACGAACTCCGGCG
TGTCCGAGCCAACCGTCCGGTCCTGCTGGATCTCCGCCTCTTTCGTTACCGCACGTTTCGATGGGGCAATCTCGCGGCCC
TGATCGTGAGTCTCGGCGAGTTCGGCATCGTCTTCGTTCTCCCGCTCTATCTCCAGGCGGTTCTGGGCTATACGGCCTTT
CAAACCGGCCTGGTCCTGCTGGCTACCGCCATCGGCGCTTTCCTCGGTGGACCAGCGGCGGCTAACCTGGCTCAGCGTTT
CGGAGCGCGTCAGGTCATCCGCGCCGGTATGCTCTTCGAGGCGGTCGGCCTCCTTTTGATCGTTCCTTTCCTCGCCCCCG
ATACCACCGGTTGGCGGCTCGCTCCGGCCTTGTTTCTTTACGGCCTCGGGGTCGGGCTCGCAACGGCGCAGCTCACCTCC
GTCATCCTCGCTGAAGTGCCCACAGCTCAGTCTGGCCAAGCTTCCGGAACCCAGAGCACGACACGCCAGGTGGGTGCCGC
GCTGGGTATCGCACTGCTCGGCACCATCCTGGCTGCCGGCCTCGGCAGCGGAACGAAGTCGCGCCTGATCGATCAACTCG
GCATCCCCCCGCAGCAGGCCGCCGCACTGGCACGTGCGGCCAAGCAATCCGCCGGCCAGATTCTCGTCCAGTTACAGACC
CAACCCGGTACGCAGCCGATTGTCCAAGCCATCGAGGCTGCCTTCACCGACGCTGCCCGGCGCACCGCATTGGTCGCTGC
CGCCTTCATCCTCGTCGGCTTTATCGCCAGTTGGCTGATTCCTGATATCCGGCCAGAACGCGGGCCGACTGCCGTGAGCG
AGCCGGCCACCGCTGAATCCCGTCCAACAGGATGA

Upstream 100 bases:

>100_bases
CAGGGAGGTATTCGGGCAAGAACCCAAACGGTGTGGTACAATTCGAGCAGGTCGAACAGAGTGAGTGTTTGGTAGTTTTG
GGCAAGATTGGAGCGCTTCG

Downstream 100 bases:

>100_bases
AACTCTCAGCGAGCGAACGAAACGACGAGCAAGAGCACGAGGCCCAGTGCGATGCGGTACACGATGAAGATGCTCGTACT
GTAGCGCTGCAGGAAGCGCA

Product: putative transport protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTAAPRTRWLGLASLALGLAMIIVDATIVNVAIPSIIEDLGLQLTDAEWVNTVYSLVFAALLVTVGRLGDLYGRKRIYLG
GLALFVGASLLCASAPSGTLLIAARALQGIGAACILPSTLSSVNAMFRGRERALAFGIWGSVIGGMAALGPLVGGWLTTD
FSWRWAFYINLPIGLVAGTIALLTVPETRDPSAAPGIDLIGIVTSAIGFAALVFGLIEGERYGWWRPTDTFRIGSWEWPF
ATLSPVPVALALAALFLPTFALYELRRVRANRPVLLDLRLFRYRTFRWGNLAALIVSLGEFGIVFVLPLYLQAVLGYTAF
QTGLVLLATAIGAFLGGPAAANLAQRFGARQVIRAGMLFEAVGLLLIVPFLAPDTTGWRLAPALFLYGLGVGLATAQLTS
VILAEVPTAQSGQASGTQSTTRQVGAALGIALLGTILAAGLGSGTKSRLIDQLGIPPQQAAALARAAKQSAGQILVQLQT
QPGTQPIVQAIEAAFTDAARRTALVAAAFILVGFIASWLIPDIRPERGPTAVSEPATAESRPTG

Sequences:

>Translated_544_residues
MTAAPRTRWLGLASLALGLAMIIVDATIVNVAIPSIIEDLGLQLTDAEWVNTVYSLVFAALLVTVGRLGDLYGRKRIYLG
GLALFVGASLLCASAPSGTLLIAARALQGIGAACILPSTLSSVNAMFRGRERALAFGIWGSVIGGMAALGPLVGGWLTTD
FSWRWAFYINLPIGLVAGTIALLTVPETRDPSAAPGIDLIGIVTSAIGFAALVFGLIEGERYGWWRPTDTFRIGSWEWPF
ATLSPVPVALALAALFLPTFALYELRRVRANRPVLLDLRLFRYRTFRWGNLAALIVSLGEFGIVFVLPLYLQAVLGYTAF
QTGLVLLATAIGAFLGGPAAANLAQRFGARQVIRAGMLFEAVGLLLIVPFLAPDTTGWRLAPALFLYGLGVGLATAQLTS
VILAEVPTAQSGQASGTQSTTRQVGAALGIALLGTILAAGLGSGTKSRLIDQLGIPPQQAAALARAAKQSAGQILVQLQT
QPGTQPIVQAIEAAFTDAARRTALVAAAFILVGFIASWLIPDIRPERGPTAVSEPATAESRPTG
>Mature_543_residues
TAAPRTRWLGLASLALGLAMIIVDATIVNVAIPSIIEDLGLQLTDAEWVNTVYSLVFAALLVTVGRLGDLYGRKRIYLGG
LALFVGASLLCASAPSGTLLIAARALQGIGAACILPSTLSSVNAMFRGRERALAFGIWGSVIGGMAALGPLVGGWLTTDF
SWRWAFYINLPIGLVAGTIALLTVPETRDPSAAPGIDLIGIVTSAIGFAALVFGLIEGERYGWWRPTDTFRIGSWEWPFA
TLSPVPVALALAALFLPTFALYELRRVRANRPVLLDLRLFRYRTFRWGNLAALIVSLGEFGIVFVLPLYLQAVLGYTAFQ
TGLVLLATAIGAFLGGPAAANLAQRFGARQVIRAGMLFEAVGLLLIVPFLAPDTTGWRLAPALFLYGLGVGLATAQLTSV
ILAEVPTAQSGQASGTQSTTRQVGAALGIALLGTILAAGLGSGTKSRLIDQLGIPPQQAAALARAAKQSAGQILVQLQTQ
PGTQPIVQAIEAAFTDAARRTALVAAAFILVGFIASWLIPDIRPERGPTAVSEPATAESRPTG

Specific function: Promotes the efflux of actinorhodin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI87081983, Length=447, Percent_Identity=26.6219239373602, Blast_Score=140, Evalue=3e-34,
Organism=Escherichia coli, GI1789042, Length=481, Percent_Identity=25.987525987526, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1790195, Length=494, Percent_Identity=26.5182186234818, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI1788392, Length=442, Percent_Identity=28.5067873303167, Blast_Score=125, Evalue=9e-30,
Organism=Escherichia coli, GI1788710, Length=498, Percent_Identity=24.4979919678715, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1790146, Length=168, Percent_Identity=29.1666666666667, Blast_Score=72, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=560, Percent_Identity=23.3928571428571, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323520, Length=340, Percent_Identity=26.4705882352941, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324954, Length=400, Percent_Identity=23.5, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=145, Percent_Identity=30.3448275862069, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323735, Length=495, Percent_Identity=25.2525252525253, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=232, Percent_Identity=26.7241379310345, Blast_Score=64, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57020; Mature: 56888

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAAPRTRWLGLASLALGLAMIIVDATIVNVAIPSIIEDLGLQLTDAEWVNTVYSLVFAA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHH
LLVTVGRLGDLYGRKRIYLGGLALFVGASLLCASAPSGTLLIAARALQGIGAACILPSTL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCCCHHHHHHHH
SSVNAMFRGRERALAFGIWGSVIGGMAALGPLVGGWLTTDFSWRWAFYINLPIGLVAGTI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEEECCHHHHHHHH
ALLTVPETRDPSAAPGIDLIGIVTSAIGFAALVFGLIEGERYGWWRPTDTFRIGSWEWPF
HEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCC
ATLSPVPVALALAALFLPTFALYELRRVRANRPVLLDLRLFRYRTFRWGNLAALIVSLGE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHH
FGIVFVLPLYLQAVLGYTAFQTGLVLLATAIGAFLGGPAAANLAQRFGARQVIRAGMLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHH
AVGLLLIVPFLAPDTTGWRLAPALFLYGLGVGLATAQLTSVILAEVPTAQSGQASGTQST
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
TRQVGAALGIALLGTILAAGLGSGTKSRLIDQLGIPPQQAAALARAAKQSAGQILVQLQT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEC
QPGTQPIVQAIEAAFTDAARRTALVAAAFILVGFIASWLIPDIRPERGPTAVSEPATAES
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
RPTG
CCCC
>Mature Secondary Structure 
TAAPRTRWLGLASLALGLAMIIVDATIVNVAIPSIIEDLGLQLTDAEWVNTVYSLVFAA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEECCHHHHHHHHHHHHHH
LLVTVGRLGDLYGRKRIYLGGLALFVGASLLCASAPSGTLLIAARALQGIGAACILPSTL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHCCCCHHHHHHHH
SSVNAMFRGRERALAFGIWGSVIGGMAALGPLVGGWLTTDFSWRWAFYINLPIGLVAGTI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCEEEEEEEECCHHHHHHHH
ALLTVPETRDPSAAPGIDLIGIVTSAIGFAALVFGLIEGERYGWWRPTDTFRIGSWEWPF
HEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEECCCCCCC
ATLSPVPVALALAALFLPTFALYELRRVRANRPVLLDLRLFRYRTFRWGNLAALIVSLGE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHCCCCHHHHHHHHHHH
FGIVFVLPLYLQAVLGYTAFQTGLVLLATAIGAFLGGPAAANLAQRFGARQVIRAGMLFE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHHHHHH
AVGLLLIVPFLAPDTTGWRLAPALFLYGLGVGLATAQLTSVILAEVPTAQSGQASGTQST
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHH
TRQVGAALGIALLGTILAAGLGSGTKSRLIDQLGIPPQQAAALARAAKQSAGQILVQLQT
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEEEEEEC
QPGTQPIVQAIEAAFTDAARRTALVAAAFILVGFIASWLIPDIRPERGPTAVSEPATAES
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
RPTG
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1878971; 12000953 [H]