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The map label for this gene is nadE2 [H]

Identifier: 221632485

GI number: 221632485

Start: 453510

End: 455270

Strand: Reverse

Name: nadE2 [H]

Synonym: trd_0459

Alternate gene names: 221632485

Gene position: 455270-453510 (Counterclockwise)

Preceding gene: 221632489

Following gene: 221632484

Centisome position: 22.73

GC content: 60.99

Gene sequence:

>1761_bases
GTGGTCGAGGAGATGACGCGCGACACGTGGCGGATCGCGCTCGCGCAGATCGATCCGACAGTCGGCGACTTCTCGGGCAA
CGTCCGCCTCATCCGCGAGCGCGCCCGGGAAGCGGCCCAACTCGGTGCTCATATCGTTGCTTTTCCCGAACTCGTCATCA
CCGGTTATCCTCCAGAAGATCTGCTCCTCAAGGTCAGCTTCATCGACGCGGCTCGCCGCGCGCTTCATCTGCTCCTCGAC
GCCATACCGGATCGCGTCCTGATCGTCGGTGTACCATGGGTCGAGAGCGGCATCCTCTATAATGGGGCCGCTATCCTCGC
CCGTGGTGAGATCGTCGCCGTTGTCCCCAAACACCATCTGCCGACCTATGGTGTCTTCGACGAGGATCGCTATTTCGCAC
GCGGAAGTCACACGTTTCGGTTTCTCTGGGGCAATCTTCGGTTCGGCGTCACCATCTGCGAAGATATCTGGTACCCGATC
GGACCAGCCAGCAGCCTGGCCACCAGAGGGATCGACTTGCTCGTCAACATCAATGGTTCGCCGTACCATCGCGGGAAGTG
GATACAGCGCCAGGTGATGCTCCAAACCCGTGCCGCCGATGCCGGCTGTTATCTGGCCTACGTCAACATGGTCGGCGGGC
AGGACGAACTGGTCTTCGACGGCAACAGCATCGTGCTCGACCCCAACGGGACGATCCTCGCCCGTGGAACATCCTTCGAA
GAGGATCTCATTGTCGTCGACCTTCCGATCACGCGCGTGCTTTCCAGCTGGCTGCACGACCCGCGCCGACGCTGGCTCGC
CCGAGCAGAGTCGCAACCTGCGCTTCCTGTGGTCGATCGCGAGATTCCCCTACCCGTCCCTGGGGAACAGCTTCCACCCT
TGCCGGAGAGCGTCCAGCGGCCACGCACGCCGCTCGAGGGAGTCGCGGAAGTCTACCAGGCTCTCGTCACCGGTGTTCGC
GACTATGTGCGCAAAACCGGATTCCGCACCGCCGTGATCGGCTTATCGGGAGGGATCGATTCGTCTCTGACAGCATGCAT
CGCCGCGGACGCCCTCGGTCCGGAGAATGTCACTGGCCTCTCGATGCCCAGCCGATACTCTTCGCGCCACAGCATCGAGG
ATGCCGAAGAGCTGGCCCGCAACCTCGGTATTCGCTTTCTGATCGTACCCATCGAACCGGTCCATCGTGTTTTCCGCGAA
GTGCTGGCACCGCTCGGTAACCAGCCAGAAGAGCCGGATGTGGCAGACGAAAATCTGCAAGCGCGTATCCGCGGCACGAT
TCTCATGACGTATTCCAATCGTTTCGGGCCGATCGTGCTCACCACGGGGAACAAGTCCGAAATGGCCTGTGGCTATGCGA
CGCTTTACGGCGATATGGCCGGCGGCTTCGCCGTCCTCAAAGATGTCCCGAAACTCCTCGTCTACCAACTCGCACGCTAT
CGCAACAGCATCTCGCCGGTCATTCCCGAGCGTGTCTTCGTCAAGCCACCGTCTGCGGAACTTCGCCCGAATCAGAAGGA
CGTCGATACGCTTCCGCCCTTCGAAATTCTCGATCCACTCCTGGAGCTCTACGTCGAGCAGGATCTCAGCCGAGAGGAAT
TGATCCGGCATGGCTTCGATCCTGAAATCGTCGACCGCGTCATCATGATGGTGGATCGCGCCGAGTACAAACGTCGACAA
GCAGCACCAGGGGTCAAGCTGACGCCGCGCGCCTTCGGACGCGATCGCCGCTTACCCATTGCCAACTGGTTCCATGGCTG
A

Upstream 100 bases:

>100_bases
ACTCGTTCTGGATTCTCTCCGAATTGTACGGCATAGATGCATTTCCGCTCTTGTTACACTGAGCGGCAGCGAACAGTAGA
CACGAGGGGAGGTACCGCGA

Downstream 100 bases:

>100_bases
CATCACGTTGTCAGGCGACCGGCCATCCTGGTACAGTTGCGCTACGCAACGCATCGTGATCGTTCTCACGAGTTTCGAGC
GGAGTCGGAGGGCGAGAGGT

Product: glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 586; Mature: 586

Protein sequence:

>586_residues
MVEEMTRDTWRIALAQIDPTVGDFSGNVRLIRERAREAAQLGAHIVAFPELVITGYPPEDLLLKVSFIDAARRALHLLLD
AIPDRVLIVGVPWVESGILYNGAAILARGEIVAVVPKHHLPTYGVFDEDRYFARGSHTFRFLWGNLRFGVTICEDIWYPI
GPASSLATRGIDLLVNINGSPYHRGKWIQRQVMLQTRAADAGCYLAYVNMVGGQDELVFDGNSIVLDPNGTILARGTSFE
EDLIVVDLPITRVLSSWLHDPRRRWLARAESQPALPVVDREIPLPVPGEQLPPLPESVQRPRTPLEGVAEVYQALVTGVR
DYVRKTGFRTAVIGLSGGIDSSLTACIAADALGPENVTGLSMPSRYSSRHSIEDAEELARNLGIRFLIVPIEPVHRVFRE
VLAPLGNQPEEPDVADENLQARIRGTILMTYSNRFGPIVLTTGNKSEMACGYATLYGDMAGGFAVLKDVPKLLVYQLARY
RNSISPVIPERVFVKPPSAELRPNQKDVDTLPPFEILDPLLELYVEQDLSREELIRHGFDPEIVDRVIMMVDRAEYKRRQ
AAPGVKLTPRAFGRDRRLPIANWFHG

Sequences:

>Translated_586_residues
MVEEMTRDTWRIALAQIDPTVGDFSGNVRLIRERAREAAQLGAHIVAFPELVITGYPPEDLLLKVSFIDAARRALHLLLD
AIPDRVLIVGVPWVESGILYNGAAILARGEIVAVVPKHHLPTYGVFDEDRYFARGSHTFRFLWGNLRFGVTICEDIWYPI
GPASSLATRGIDLLVNINGSPYHRGKWIQRQVMLQTRAADAGCYLAYVNMVGGQDELVFDGNSIVLDPNGTILARGTSFE
EDLIVVDLPITRVLSSWLHDPRRRWLARAESQPALPVVDREIPLPVPGEQLPPLPESVQRPRTPLEGVAEVYQALVTGVR
DYVRKTGFRTAVIGLSGGIDSSLTACIAADALGPENVTGLSMPSRYSSRHSIEDAEELARNLGIRFLIVPIEPVHRVFRE
VLAPLGNQPEEPDVADENLQARIRGTILMTYSNRFGPIVLTTGNKSEMACGYATLYGDMAGGFAVLKDVPKLLVYQLARY
RNSISPVIPERVFVKPPSAELRPNQKDVDTLPPFEILDPLLELYVEQDLSREELIRHGFDPEIVDRVIMMVDRAEYKRRQ
AAPGVKLTPRAFGRDRRLPIANWFHG
>Mature_586_residues
MVEEMTRDTWRIALAQIDPTVGDFSGNVRLIRERAREAAQLGAHIVAFPELVITGYPPEDLLLKVSFIDAARRALHLLLD
AIPDRVLIVGVPWVESGILYNGAAILARGEIVAVVPKHHLPTYGVFDEDRYFARGSHTFRFLWGNLRFGVTICEDIWYPI
GPASSLATRGIDLLVNINGSPYHRGKWIQRQVMLQTRAADAGCYLAYVNMVGGQDELVFDGNSIVLDPNGTILARGTSFE
EDLIVVDLPITRVLSSWLHDPRRRWLARAESQPALPVVDREIPLPVPGEQLPPLPESVQRPRTPLEGVAEVYQALVTGVR
DYVRKTGFRTAVIGLSGGIDSSLTACIAADALGPENVTGLSMPSRYSSRHSIEDAEELARNLGIRFLIVPIEPVHRVFRE
VLAPLGNQPEEPDVADENLQARIRGTILMTYSNRFGPIVLTTGNKSEMACGYATLYGDMAGGFAVLKDVPKLLVYQLARY
RNSISPVIPERVFVKPPSAELRPNQKDVDTLPPFEILDPLLELYVEQDLSREELIRHGFDPEIVDRVIMMVDRAEYKRRQ
AAPGVKLTPRAFGRDRRLPIANWFHG

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI41393551, Length=585, Percent_Identity=23.0769230769231, Blast_Score=108, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17538756, Length=588, Percent_Identity=25.3401360544218, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 65273; Mature: 65273

Theoretical pI: Translated: 6.01; Mature: 6.01

Prosite motif: PS00920 NITRIL_CHT_1 ; PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEEMTRDTWRIALAQIDPTVGDFSGNVRLIRERAREAAQLGAHIVAFPELVITGYPPED
CCCHHHCHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECEEEEECCCCHH
LLLKVSFIDAARRALHLLLDAIPDRVLIVGVPWVESGILYNGAAILARGEIVAVVPKHHL
HEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCEEECCEEEEECCCEEEEECCCCC
PTYGVFDEDRYFARGSHTFRFLWGNLRFGVTICEDIWYPIGPASSLATRGIDLLVNINGS
CCCCCCCCCCEEECCCCEEEEEEECCEECEEEHHHCCCCCCCHHHHHHCCEEEEEECCCC
PYHRGKWIQRQVMLQTRAADAGCYLAYVNMVGGQDELVFDGNSIVLDPNGTILARGTSFE
CCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCEEEECCCCEEEECCCCCC
EDLIVVDLPITRVLSSWLHDPRRRWLARAESQPALPVVDREIPLPVPGEQLPPLPESVQR
CCEEEEECCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
PRTPLEGVAEVYQALVTGVRDYVRKTGFRTAVIGLSGGIDSSLTACIAADALGPENVTGL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
SMPSRYSSRHSIEDAEELARNLGIRFLIVPIEPVHRVFREVLAPLGNQPEEPDVADENLQ
CCCCHHCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
ARIRGTILMTYSNRFGPIVLTTGNKSEMACGYATLYGDMAGGFAVLKDVPKLLVYQLARY
EEEEEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RNSISPVIPERVFVKPPSAELRPNQKDVDTLPPFEILDPLLELYVEQDLSREELIRHGFD
HCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
PEIVDRVIMMVDRAEYKRRQAAPGVKLTPRAFGRDRRLPIANWFHG
HHHHHHHHHHHHHHHHHHHHCCCCCEECHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MVEEMTRDTWRIALAQIDPTVGDFSGNVRLIRERAREAAQLGAHIVAFPELVITGYPPED
CCCHHHCHHHEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHCCEEEEECEEEEECCCCHH
LLLKVSFIDAARRALHLLLDAIPDRVLIVGVPWVESGILYNGAAILARGEIVAVVPKHHL
HEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCEEECCEEEEECCCEEEEECCCCC
PTYGVFDEDRYFARGSHTFRFLWGNLRFGVTICEDIWYPIGPASSLATRGIDLLVNINGS
CCCCCCCCCCEEECCCCEEEEEEECCEECEEEHHHCCCCCCCHHHHHHCCEEEEEECCCC
PYHRGKWIQRQVMLQTRAADAGCYLAYVNMVGGQDELVFDGNSIVLDPNGTILARGTSFE
CCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEECCCEEEECCCCEEEECCCCCC
EDLIVVDLPITRVLSSWLHDPRRRWLARAESQPALPVVDREIPLPVPGEQLPPLPESVQR
CCEEEEECCHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHC
PRTPLEGVAEVYQALVTGVRDYVRKTGFRTAVIGLSGGIDSSLTACIAADALGPENVTGL
CCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCCCCCCC
SMPSRYSSRHSIEDAEELARNLGIRFLIVPIEPVHRVFREVLAPLGNQPEEPDVADENLQ
CCCCHHCCCCCHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCCCCCE
ARIRGTILMTYSNRFGPIVLTTGNKSEMACGYATLYGDMAGGFAVLKDVPKLLVYQLARY
EEEEEEEEEEECCCCCCEEEECCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
RNSISPVIPERVFVKPPSAELRPNQKDVDTLPPFEILDPLLELYVEQDLSREELIRHGFD
HCCCCCCCCCCEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHCCCC
PEIVDRVIMMVDRAEYKRRQAAPGVKLTPRAFGRDRRLPIANWFHG
HHHHHHHHHHHHHHHHHHHHCCCCCEECHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]