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The map label for this gene is otsA [H]

Identifier: 221632399

GI number: 221632399

Start: 375234

End: 376826

Strand: Reverse

Name: otsA [H]

Synonym: trd_0371

Alternate gene names: 221632399

Gene position: 376826-375234 (Counterclockwise)

Preceding gene: 221632401

Following gene: 221632398

Centisome position: 18.81

GC content: 62.34

Gene sequence:

>1593_bases
ATGGCACACCAGCGAGCGGGGCAGCACCGGGATGGTCAGCTGGACGTGCAGCTCCCCGACTACGCGCGCGAGCTCCTCGA
GCGCTCGACCTTGATCATCGTCTCCAACCGCGGACCGGTCGAGTTCCACCAGACCAACGACGGCGAGATCGCCACGAAGC
GGGGCACAGGCGGAGTTATTACTGCTATGAGCGCCGCAGCACGCTTCGCCGACGCCATTTGGGTGGCCTGCGCGATGACG
CCCACTGATCGCGTCATCGCGGAGGAAATGCGGCAACGGCGAGAAGCGCTCCTCACGATACCGGATCTGCCTGGTTTCCG
ACTTCGCTTCGTCGTTCCCGATACCGAAGCGTACCGTGGCTATTACAACCGGATTGCCAATCCATTGCTCTGGTTCCTGC
AGCATTATCTTTGGGATACTCCTCGCGCGCCCGATATCGACCACGAGACGTGGCAAGCCTGGCACGAGGGATACGTTGCG
GTCAACCGCCTCTTCGCTGAAGAGGTGGTGGCCGCCGCAGCGGCGGCCCCACGCGATCCCATTGTGCTCTTGCAGGACTA
TCACCTCTACCTCGCCCCGACCTTCATCCGCGAGTTGCGCCCTGACCTGCTCCTGCACCACTTCGTTCATATCCCGTGGC
CGGACCAGGACTATTGGCGATTGCTCCCACTTCCCATGCGCCGTGCGATCTGCGAGGGCCTGCTGGCCAACGATATCGTC
GGATTCCAAACGCCCCGCCACGCCCGGAGCTTCCTCAATACGGTCGATGCGAATTTGCCAGAAGCAGAAGTCGACTATCG
GCGGCGCGAAGTGACCTATCGCGGGCATACGACCCGCGTCCGCGTCTATCCGATCTCGATCGACCCCGAACTGGTCCGGG
CCGTAGCCGAAAGCGAAGATGCCCGTCGGCATCGTGAACACCTCGAGGCGTTCCGCAACGAATACACCATCATCCGGGTC
GATCGAGCCGAACCGAGCAAGAACGTGGTGCGCGGCTTCCTGGCGTACGACCGCTTGCTCGAAAAGCACCCCGAGTTGAT
CGGGCGCGTCAACTTTCTCGCTTTTCTCGTCCCGACACGGATGGAGGTTGCCGAATACATCAACTACCTCGACGAGATCA
ATGCAGTTGTCGGCCGCATCAACGCCAAGTACGCGAACGCTGCGGAGATCGAGGGCATCCACTGGGAGCCTGTGCACCTC
TTCGTCGGTGACGACTATCCCCGAGCACTCGCTGCGATGAAGTTCTACGACGTGCTCTTGGTCAATGCGATCTTCGACGG
CATGAATCTGGTAGCCAAGGAGGGAGCGCTGCTCAACGAGCGCAATGGAGTCCTCATCCTCTCGGAAGGGGCCGGAGCAT
ACCAGCAACTCGGGCAGTGGGCATTGACCGTCTCGCCCACGGATATCGAAGGCACAGCGGAGGCCCTCTGGCAGGGGCTG
ATAATGCCTGCCGCAGAACGGGCCTGGCGGGCCGAACAGTTGCGCCGTCACGTCGTCGAGCATGATCTCCGCCGTTGGCT
CGTCGATCAGCTGCACGACATCGCCGACCTCCGCGCCGAGCGCGCGCCGCACCGCCTCATCGCCCCTTCTTGA

Upstream 100 bases:

>100_bases
GCGTCGCCGTGGAGCGGTCGGAACGACGATCGGTCCAGGAACGACATCTCCATGACTTGGCCACGGACCACAGCTGGGAC
GACCAGAGGGGAGGACAACG

Downstream 100 bases:

>100_bases
CCGAGCTGCTTCCCCAGACGGGGCTTTGGTACAACTCATCCGGCGCGGGCTGCATCCGTCGCCAGGAGGGACAATGGTGT
TCGAGCGCATCGGTCGATTT

Product: trehalose-6-phosphate synthase

Products: UDP; alpha,alpha'-Trehalose 6-phosphate

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MAHQRAGQHRDGQLDVQLPDYARELLERSTLIIVSNRGPVEFHQTNDGEIATKRGTGGVITAMSAAARFADAIWVACAMT
PTDRVIAEEMRQRREALLTIPDLPGFRLRFVVPDTEAYRGYYNRIANPLLWFLQHYLWDTPRAPDIDHETWQAWHEGYVA
VNRLFAEEVVAAAAAAPRDPIVLLQDYHLYLAPTFIRELRPDLLLHHFVHIPWPDQDYWRLLPLPMRRAICEGLLANDIV
GFQTPRHARSFLNTVDANLPEAEVDYRRREVTYRGHTTRVRVYPISIDPELVRAVAESEDARRHREHLEAFRNEYTIIRV
DRAEPSKNVVRGFLAYDRLLEKHPELIGRVNFLAFLVPTRMEVAEYINYLDEINAVVGRINAKYANAAEIEGIHWEPVHL
FVGDDYPRALAAMKFYDVLLVNAIFDGMNLVAKEGALLNERNGVLILSEGAGAYQQLGQWALTVSPTDIEGTAEALWQGL
IMPAAERAWRAEQLRRHVVEHDLRRWLVDQLHDIADLRAERAPHRLIAPS

Sequences:

>Translated_530_residues
MAHQRAGQHRDGQLDVQLPDYARELLERSTLIIVSNRGPVEFHQTNDGEIATKRGTGGVITAMSAAARFADAIWVACAMT
PTDRVIAEEMRQRREALLTIPDLPGFRLRFVVPDTEAYRGYYNRIANPLLWFLQHYLWDTPRAPDIDHETWQAWHEGYVA
VNRLFAEEVVAAAAAAPRDPIVLLQDYHLYLAPTFIRELRPDLLLHHFVHIPWPDQDYWRLLPLPMRRAICEGLLANDIV
GFQTPRHARSFLNTVDANLPEAEVDYRRREVTYRGHTTRVRVYPISIDPELVRAVAESEDARRHREHLEAFRNEYTIIRV
DRAEPSKNVVRGFLAYDRLLEKHPELIGRVNFLAFLVPTRMEVAEYINYLDEINAVVGRINAKYANAAEIEGIHWEPVHL
FVGDDYPRALAAMKFYDVLLVNAIFDGMNLVAKEGALLNERNGVLILSEGAGAYQQLGQWALTVSPTDIEGTAEALWQGL
IMPAAERAWRAEQLRRHVVEHDLRRWLVDQLHDIADLRAERAPHRLIAPS
>Mature_529_residues
AHQRAGQHRDGQLDVQLPDYARELLERSTLIIVSNRGPVEFHQTNDGEIATKRGTGGVITAMSAAARFADAIWVACAMTP
TDRVIAEEMRQRREALLTIPDLPGFRLRFVVPDTEAYRGYYNRIANPLLWFLQHYLWDTPRAPDIDHETWQAWHEGYVAV
NRLFAEEVVAAAAAAPRDPIVLLQDYHLYLAPTFIRELRPDLLLHHFVHIPWPDQDYWRLLPLPMRRAICEGLLANDIVG
FQTPRHARSFLNTVDANLPEAEVDYRRREVTYRGHTTRVRVYPISIDPELVRAVAESEDARRHREHLEAFRNEYTIIRVD
RAEPSKNVVRGFLAYDRLLEKHPELIGRVNFLAFLVPTRMEVAEYINYLDEINAVVGRINAKYANAAEIEGIHWEPVHLF
VGDDYPRALAAMKFYDVLLVNAIFDGMNLVAKEGALLNERNGVLILSEGAGAYQQLGQWALTVSPTDIEGTAEALWQGLI
MPAAERAWRAEQLRRHVVEHDLRRWLVDQLHDIADLRAERAPHRLIAPS

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=495, Percent_Identity=28.0808080808081, Blast_Score=149, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI115533560, Length=500, Percent_Identity=27.4, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI115533558, Length=500, Percent_Identity=27.4, Blast_Score=136, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI71987012, Length=583, Percent_Identity=24.8713550600343, Blast_Score=129, Evalue=5e-30,
Organism=Saccharomyces cerevisiae, GI6319602, Length=508, Percent_Identity=28.9370078740157, Blast_Score=182, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6323917, Length=466, Percent_Identity=28.3261802575107, Blast_Score=152, Evalue=9e-38,
Organism=Saccharomyces cerevisiae, GI6323537, Length=481, Percent_Identity=25.3638253638254, Blast_Score=149, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320279, Length=391, Percent_Identity=24.0409207161125, Blast_Score=99, Evalue=1e-21,
Organism=Drosophila melanogaster, GI19920676, Length=528, Percent_Identity=29.7348484848485, Blast_Score=190, Evalue=2e-48,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15

Molecular weight: Translated: 60778; Mature: 60647

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHQRAGQHRDGQLDVQLPDYARELLERSTLIIVSNRGPVEFHQTNDGEIATKRGTGGVI
CCCCCCCCCCCCEEEEECCHHHHHHHCCCEEEEEECCCCEEEEECCCCCEEEECCCCCHH
TAMSAAARFADAIWVACAMTPTDRVIAEEMRQRREALLTIPDLPGFRLRFVVPDTEAYRG
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCHHHHHH
YYNRIANPLLWFLQHYLWDTPRAPDIDHETWQAWHEGYVAVNRLFAEEVVAAAAAAPRDP
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IVLLQDYHLYLAPTFIRELRPDLLLHHFVHIPWPDQDYWRLLPLPMRRAICEGLLANDIV
EEEEECCHHEEHHHHHHHHCHHHHHHHHEECCCCCCCCCEECCHHHHHHHHHHHHHHCCC
GFQTPRHARSFLNTVDANLPEAEVDYRRREVTYRGHTTRVRVYPISIDPELVRAVAESED
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCEEEEEEEEECCCHHHHHHHHHHHH
ARRHREHLEAFRNEYTIIRVDRAEPSKNVVRGFLAYDRLLEKHPELIGRVNFLAFLVPTR
HHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
MEVAEYINYLDEINAVVGRINAKYANAAEIEGIHWEPVHLFVGDDYPRALAAMKFYDVLL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
VNAIFDGMNLVAKEGALLNERNGVLILSEGAGAYQQLGQWALTVSPTDIEGTAEALWQGL
HHHHHHCHHHHHHCCCEEECCCCEEEEECCCCHHHHCCCEEEEECCCCCCHHHHHHHHHH
IMPAAERAWRAEQLRRHVVEHDLRRWLVDQLHDIADLRAERAPHRLIAPS
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
AHQRAGQHRDGQLDVQLPDYARELLERSTLIIVSNRGPVEFHQTNDGEIATKRGTGGVI
CCCCCCCCCCCEEEEECCHHHHHHHCCCEEEEEECCCCEEEEECCCCCEEEECCCCCHH
TAMSAAARFADAIWVACAMTPTDRVIAEEMRQRREALLTIPDLPGFRLRFVVPDTEAYRG
HHHHHHHHHHHHHHHEEECCCHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCHHHHHH
YYNRIANPLLWFLQHYLWDTPRAPDIDHETWQAWHEGYVAVNRLFAEEVVAAAAAAPRDP
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
IVLLQDYHLYLAPTFIRELRPDLLLHHFVHIPWPDQDYWRLLPLPMRRAICEGLLANDIV
EEEEECCHHEEHHHHHHHHCHHHHHHHHEECCCCCCCCCEECCHHHHHHHHHHHHHHCCC
GFQTPRHARSFLNTVDANLPEAEVDYRRREVTYRGHTTRVRVYPISIDPELVRAVAESED
CCCCCHHHHHHHHHHCCCCCCHHHHHHHHHEEECCCEEEEEEEEECCCHHHHHHHHHHHH
ARRHREHLEAFRNEYTIIRVDRAEPSKNVVRGFLAYDRLLEKHPELIGRVNFLAFLVPTR
HHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHH
MEVAEYINYLDEINAVVGRINAKYANAAEIEGIHWEPVHLFVGDDYPRALAAMKFYDVLL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHHH
VNAIFDGMNLVAKEGALLNERNGVLILSEGAGAYQQLGQWALTVSPTDIEGTAEALWQGL
HHHHHHCHHHHHHCCCEEECCCCEEEEECCCCHHHHCCCEEEEECCCCCCHHHHHHHHHH
IMPAAERAWRAEQLRRHVVEHDLRRWLVDQLHDIADLRAERAPHRLIAPS
HCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-glucose; D-glucose 6-phosphate

Specific reaction: UDP-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA