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The map label for this gene is nadB [H]

Identifier: 221632360

GI number: 221632360

Start: 333866

End: 335596

Strand: Reverse

Name: nadB [H]

Synonym: trd_0331

Alternate gene names: 221632360

Gene position: 335596-333866 (Counterclockwise)

Preceding gene: 221632361

Following gene: 221632359

Centisome position: 16.75

GC content: 62.1

Gene sequence:

>1731_bases
ATGGTGTTCCATCGCTTCGATGCGGTCATCGTCGGCGCGGGTGGAGCCGGCCTGATGGCCGCCGCTCAGCTGGCAGGCAA
ATGCAATGTAGCCGTCGTCAGCAAACTGTATCCGGTTCGCTCGCACACCGGTGCTGCTCAGGGTGGGATCGCCGCTGCCC
TCGGGAACGTCGAGGAGGACCACTGGATCTGGCACATGTTCGACACCGTCAAGGGCGGCGACTATCTCGTCGATCAGGAT
GCGGCCGAAGTCCTCGCCCGCGAGGCGATCGACGCCATCATCGAACTGGAGCATTGGGGATTTCCCTTCAACCGGACACC
GGATGGTCGGATCGATCAGCGGCGCTTCGGTGGTCACACCCGTAACTTCGGTGAGGGTCCAGTGCGCCGGGCGTGCTATG
CGGCTGATCGTACCGGCCACATGATCCTTCAAACGCTCTACCAGACTGCGCTCAAGCGGCAGGTCACCTTCTTCGACGAG
TTCATGATGCTCGATCTCTTGCTGACCGACGACGGTGTCTGCACCGGCATCGTCGCTATGGATATCGAGACCGGCGACAT
CCATGTCTTCCACGCCAAAGCCGTGCTCCTGGCGACCGGTGGCTTCGGGCGTGTCTTCAAGGTCACGAGCAATGCCCTCG
CGGCAACGGGTGACGGCGTGGCCATTGCCTTTCGGCGCGGTATTCCCCTGGAAGACATGGAGTTCTACCAGTTCCATCCG
ACGGGAATCTACAAGATGGGGATCCTCCTCTCGGAAGCCTGCCGCGGGGAGGGCGGAATCTTGCGCAACGGACTGGGCGA
GCGCTTCATGGAGCGCTACGCCCCGACGCTCCTCGACTTGGCACCACGCGACATGGTTTCCCGTGCCATCTATCAGGAGA
TTCGCGCCGGTCGTGGTGTGGACGGCAAGGACTACGTCTGGCTCGATCTGACTCATCTCCCGGCTGAGGTCATCGAGACG
AAACTCCCTGACATCACCGACTTCGTGCGGACGTACCTTGGCCTCGATCCCGTCAAGGATCTTATCCCCATCCAGCCGAC
AGCCCACTACGCGATGGGGGGGATCCCCACCGATGTCGACGGCCGAGTGACGATCGATGCGCAGAACACGCCCATACCCG
GTCTCTACGCGGCGGGCGAGTGTGCCTGTGTGAGCGTCCACGGTGCGAACCGGCTCGGCACCAACTCGCTCGTCGATCTC
GTCGTCTTCGGTCGACGCGCTGGTCGCCACATGGCACGCTTCGTCCAGGAGAACGACTTCCATCCACTGCCGAAAGACCC
GGCCTACCGCGCTCGTGCCGAAATCGAGTGGATCCGGGAGAACGACGGGACCGAGCGTGTCGCCCAAATCCGCGCCGAGT
TGCAGGAAGCGATGATGACCGACGCAGGTGTCTTTCGCACCGGCGACGGTCTGAAGCGCTTGCAGCAGAAGCTCGGTGAG
CTACGCAATCGCTACACGCAGATTCGGATCGACGACAAGAGTCGCACCTTCAACATGGAACTGATGGAAGCGATCGAGCT
CGGCTTCCTGCTCGACTGTGCCGAAGCCATCGCTGCTTCGGCGCTGGCTCGCGAGGAAAGCCGGGGTGCTCACTATCGCG
AAGACTTCCCAGCACGGGACGATGCCAACTGGCTCAAGCACACCCTGATCGCTCGGACATCAGGTGGACTCCAGCTCTTC
TACAAGCCAGTCGTCATCACGCGGTTCGAGCCGAAGGAACGGGTGTACTGA

Upstream 100 bases:

>100_bases
CTGGACCTTGGCCCTTCTCTTTCTGGTCATCGGCTCGATCGCGATCGTGACTTTCGACCCATCCAAGTTCGAAGTCGTCG
CACAGGCAGGAGGCTGAGCC

Downstream 100 bases:

>100_bases
GATGCAGGTGACGTTGCGTATCCAACGGTTCAATCCCGAAACCGATACCAAACCGTACTACCGCGAGTACACGGTCGAGG
TCGAACCGACTGATCGGGTC

Product: succinate dehydrogenase, flavoprotein subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MVFHRFDAVIVGAGGAGLMAAAQLAGKCNVAVVSKLYPVRSHTGAAQGGIAAALGNVEEDHWIWHMFDTVKGGDYLVDQD
AAEVLAREAIDAIIELEHWGFPFNRTPDGRIDQRRFGGHTRNFGEGPVRRACYAADRTGHMILQTLYQTALKRQVTFFDE
FMMLDLLLTDDGVCTGIVAMDIETGDIHVFHAKAVLLATGGFGRVFKVTSNALAATGDGVAIAFRRGIPLEDMEFYQFHP
TGIYKMGILLSEACRGEGGILRNGLGERFMERYAPTLLDLAPRDMVSRAIYQEIRAGRGVDGKDYVWLDLTHLPAEVIET
KLPDITDFVRTYLGLDPVKDLIPIQPTAHYAMGGIPTDVDGRVTIDAQNTPIPGLYAAGECACVSVHGANRLGTNSLVDL
VVFGRRAGRHMARFVQENDFHPLPKDPAYRARAEIEWIRENDGTERVAQIRAELQEAMMTDAGVFRTGDGLKRLQQKLGE
LRNRYTQIRIDDKSRTFNMELMEAIELGFLLDCAEAIAASALAREESRGAHYREDFPARDDANWLKHTLIARTSGGLQLF
YKPVVITRFEPKERVY

Sequences:

>Translated_576_residues
MVFHRFDAVIVGAGGAGLMAAAQLAGKCNVAVVSKLYPVRSHTGAAQGGIAAALGNVEEDHWIWHMFDTVKGGDYLVDQD
AAEVLAREAIDAIIELEHWGFPFNRTPDGRIDQRRFGGHTRNFGEGPVRRACYAADRTGHMILQTLYQTALKRQVTFFDE
FMMLDLLLTDDGVCTGIVAMDIETGDIHVFHAKAVLLATGGFGRVFKVTSNALAATGDGVAIAFRRGIPLEDMEFYQFHP
TGIYKMGILLSEACRGEGGILRNGLGERFMERYAPTLLDLAPRDMVSRAIYQEIRAGRGVDGKDYVWLDLTHLPAEVIET
KLPDITDFVRTYLGLDPVKDLIPIQPTAHYAMGGIPTDVDGRVTIDAQNTPIPGLYAAGECACVSVHGANRLGTNSLVDL
VVFGRRAGRHMARFVQENDFHPLPKDPAYRARAEIEWIRENDGTERVAQIRAELQEAMMTDAGVFRTGDGLKRLQQKLGE
LRNRYTQIRIDDKSRTFNMELMEAIELGFLLDCAEAIAASALAREESRGAHYREDFPARDDANWLKHTLIARTSGGLQLF
YKPVVITRFEPKERVY
>Mature_576_residues
MVFHRFDAVIVGAGGAGLMAAAQLAGKCNVAVVSKLYPVRSHTGAAQGGIAAALGNVEEDHWIWHMFDTVKGGDYLVDQD
AAEVLAREAIDAIIELEHWGFPFNRTPDGRIDQRRFGGHTRNFGEGPVRRACYAADRTGHMILQTLYQTALKRQVTFFDE
FMMLDLLLTDDGVCTGIVAMDIETGDIHVFHAKAVLLATGGFGRVFKVTSNALAATGDGVAIAFRRGIPLEDMEFYQFHP
TGIYKMGILLSEACRGEGGILRNGLGERFMERYAPTLLDLAPRDMVSRAIYQEIRAGRGVDGKDYVWLDLTHLPAEVIET
KLPDITDFVRTYLGLDPVKDLIPIQPTAHYAMGGIPTDVDGRVTIDAQNTPIPGLYAAGECACVSVHGANRLGTNSLVDL
VVFGRRAGRHMARFVQENDFHPLPKDPAYRARAEIEWIRENDGTERVAQIRAELQEAMMTDAGVFRTGDGLKRLQQKLGE
LRNRYTQIRIDDKSRTFNMELMEAIELGFLLDCAEAIAASALAREESRGAHYREDFPARDDANWLKHTLIARTSGGLQLF
YKPVVITRFEPKERVY

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=561, Percent_Identity=46.3458110516934, Blast_Score=500, Evalue=1e-141,
Organism=Escherichia coli, GI1786942, Length=582, Percent_Identity=50, Blast_Score=511, Evalue=1e-146,
Organism=Escherichia coli, GI1790597, Length=569, Percent_Identity=43.0579964850615, Blast_Score=428, Evalue=1e-121,
Organism=Escherichia coli, GI1788928, Length=551, Percent_Identity=34.4827586206897, Blast_Score=238, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI17550100, Length=573, Percent_Identity=46.9458987783595, Blast_Score=504, Evalue=1e-143,
Organism=Caenorhabditis elegans, GI17505833, Length=600, Percent_Identity=47, Blast_Score=491, Evalue=1e-139,
Organism=Saccharomyces cerevisiae, GI6322701, Length=583, Percent_Identity=49.2281303602058, Blast_Score=531, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6322416, Length=583, Percent_Identity=49.9142367066895, Blast_Score=530, Evalue=1e-151,
Organism=Saccharomyces cerevisiae, GI6320788, Length=488, Percent_Identity=28.0737704918033, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI17137288, Length=596, Percent_Identity=46.9798657718121, Blast_Score=504, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24655642, Length=596, Percent_Identity=46.9798657718121, Blast_Score=504, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24655647, Length=596, Percent_Identity=46.9798657718121, Blast_Score=504, Evalue=1e-143,
Organism=Drosophila melanogaster, GI24663005, Length=611, Percent_Identity=43.8625204582651, Blast_Score=456, Evalue=1e-128,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR003952
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR011281
- InterPro:   IPR014006 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.3.99.1 [H]

Molecular weight: Translated: 63889; Mature: 63889

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00504 FRD_SDH_FAD_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFHRFDAVIVGAGGAGLMAAAQLAGKCNVAVVSKLYPVRSHTGAAQGGIAAALGNVEED
CCEEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCC
HWIWHMFDTVKGGDYLVDQDAAEVLAREAIDAIIELEHWGFPFNRTPDGRIDQRRFGGHT
CEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCC
RNFGEGPVRRACYAADRTGHMILQTLYQTALKRQVTFFDEFMMLDLLLTDDGVCTGIVAM
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
DIETGDIHVFHAKAVLLATGGFGRVFKVTSNALAATGDGVAIAFRRGIPLEDMEFYQFHP
EEECCCEEEEEEEEEEEEECCCCEEEEEECCCCEECCCCEEEEEECCCCCCCCCEEEECC
TGIYKMGILLSEACRGEGGILRNGLGERFMERYAPTLLDLAPRDMVSRAIYQEIRAGRGV
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCC
DGKDYVWLDLTHLPAEVIETKLPDITDFVRTYLGLDPVKDLIPIQPTAHYAMGGIPTDVD
CCCCEEEEEEHHCCHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHCCCCCCCCC
GRVTIDAQNTPIPGLYAAGECACVSVHGANRLGTNSLVDLVVFGRRAGRHMARFVQENDF
CEEEEECCCCCCCCEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
HPLPKDPAYRARAEIEWIRENDGTERVAQIRAELQEAMMTDAGVFRTGDGLKRLQQKLGE
CCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHH
LRNRYTQIRIDDKSRTFNMELMEAIELGFLLDCAEAIAASALAREESRGAHYREDFPARD
HHHHEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DANWLKHTLIARTSGGLQLFYKPVVITRFEPKERVY
CHHHHHHHHHHCCCCCEEEEEEEEEEEEECHHHCCC
>Mature Secondary Structure
MVFHRFDAVIVGAGGAGLMAAAQLAGKCNVAVVSKLYPVRSHTGAAQGGIAAALGNVEED
CCEEEEEEEEEECCCHHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCC
HWIWHMFDTVKGGDYLVDQDAAEVLAREAIDAIIELEHWGFPFNRTPDGRIDQRRFGGHT
CEEEEEEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCC
RNFGEGPVRRACYAADRTGHMILQTLYQTALKRQVTFFDEFMMLDLLLTDDGVCTGIVAM
CCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
DIETGDIHVFHAKAVLLATGGFGRVFKVTSNALAATGDGVAIAFRRGIPLEDMEFYQFHP
EEECCCEEEEEEEEEEEEECCCCEEEEEECCCCEECCCCEEEEEECCCCCCCCCEEEECC
TGIYKMGILLSEACRGEGGILRNGLGERFMERYAPTLLDLAPRDMVSRAIYQEIRAGRGV
CCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHCCCC
DGKDYVWLDLTHLPAEVIETKLPDITDFVRTYLGLDPVKDLIPIQPTAHYAMGGIPTDVD
CCCCEEEEEEHHCCHHHHHHCCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHCCCCCCCCC
GRVTIDAQNTPIPGLYAAGECACVSVHGANRLGTNSLVDLVVFGRRAGRHMARFVQENDF
CEEEEECCCCCCCCEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
HPLPKDPAYRARAEIEWIRENDGTERVAQIRAELQEAMMTDAGVFRTGDGLKRLQQKLGE
CCCCCCCCHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHCCCCEECCHHHHHHHHHHHH
LRNRYTQIRIDDKSRTFNMELMEAIELGFLLDCAEAIAASALAREESRGAHYREDFPARD
HHHHEEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
DANWLKHTLIARTSGGLQLFYKPVVITRFEPKERVY
CHHHHHHHHHHCCCCCEEEEEEEEEEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA