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The map label for this gene is gatA [H]

Identifier: 221632338

GI number: 221632338

Start: 314011

End: 315609

Strand: Reverse

Name: gatA [H]

Synonym: trd_0309

Alternate gene names: 221632338

Gene position: 315609-314011 (Counterclockwise)

Preceding gene: 221632340

Following gene: 221632337

Centisome position: 15.76

GC content: 67.1

Gene sequence:

>1599_bases
ATGGACGATACGGCATCGGTCGCACTCGCCAGGCAAAACCTCGCGGCGTTGGGCATTCCGGTGAGCGACGAGGATCTCGA
GAAGGCCCACGCAGCAGGTTTCTTCAGCACCGCGCAGGTATTCCAGGTAATGGTGCAGCGAGACAGCGAGGTCGATGACC
TACCAGATTTCCTGACCGATCGTCTACCAGCGCCGCGATCGTGGGCCGCGACGGGAGAGGAGTCCCCTTCGGCGGCACCG
GAGATCCTCGAACTCGCCCGCGCATTGCGCAACGGCGAGACGAGCAGTCGCGCGCTCGTCGAGCGAGCGCTCGAGCAGAT
CACTCGCCGCGATCAGGAACTCAACGCCTTTCAGTTGCTGCTCCCCGAGCAGGCGCTCGCCGATGCGGAGGCGCGCGATG
CTGAACTCGCTCGCGGTATCGACCGCGGACCGCTCCATGGCCTGCCCGTGGCCTTGAAGGACCTCATCGACCTCGCGGGA
ACACCGACGACCGCCGGATCAGCTATCCTCGCCAACGCGGTGGCATCACGCGATGCGACGGTTGTCCAGCGCCTCCGAGC
CGCCGGAGCCGTGATCGTCGGGAAAACACGACTACCAGAGTTCGCCTATTCGGGAGCCTCCAACAACCCGCACTACGGCC
CGGTCCCCAATCCGCGAGACCGTCGTCGCGACAGCGGGGGATCCTCGAGTGGTTCTGCCGCTGCAGTCGCCGCTGGCCTG
GTCGTCATGGCGGTCGGCTCGGATACCGGGGGATCGATCCGCATTCCGGCGGCCTATTGCGGAATCGTCGGTCTCAAGCC
GACCTTCGGACGGGTGAGCGCGCGTGGTGTCTATCCGCTCGCCTGGTCACTCGACCACATCGGACCACTCACCCGCACCG
TGACGGACGCCGCGGTTTCTCTCGAAGCGATGGCTGGTCCGGACGAGGACGACCCACGAACTCGCGCCGTTCCACTCCCG
GATCTGGTCAGCGCCACACAAGTCGAGCGTCTCGAGCTCCGAGTGGGTGTCGTCCGCTCCACGGGTGGAGACCAACCTTT
GGCCGCGCCCGACGCGGTCGCTGCGTGCGAACGGGCAGTGGCCGCGCTCGCGGACGCTGGGGCCGACCTGATCCCGCTGG
TCCTGCCGGAACTCGAGACATTGCGTATCGTGAGTGCGGCGATCGCGCAACTCGAGGCTGCTGCGCTGCACGCACCCACC
GCGCGCGAGCGCTGGACCCACTACGGTGAGTTCTTCCGTCTCCGCCTCCTCGGCTGCTTCGCCTACCCCTGCTGGGGTTA
CGTTGCCGCCCAGCGACTGGCGAAGAAGGCTCGTGCGCGGGTCACGAGCCTTCTCGAGGAGCACGATATCGCCTTGCTGG
CTCTCCCGACAACGCCGACCACAGCCCCGCCGCTCGGGCAGTGGACAGCGCGAGCGAGCTGGCTGACCGCCCCCTTCAAC
CTGCTGGGATGGCCAGCCCTCAGCGTTCCGGCCGGCCTCGGCGATGACGGCTTGCCGATCGGTCTCCAACTGGTGGCACG
TCCGTGGCGGGAAGATCTCGTCATCGCCGCAGGAGCATTGGTCGAACGCGCGGCTCGATCGAGCCGAGGCTCGAACTGA

Upstream 100 bases:

>100_bases
GCATCATACCTGACCCTTTCGCTCGACGCACGCTGGTCGATCCGCTCCTTTCACGTTACGATGACCGTTCGGGACACGCC
CTGCGGGAAGGGAGAGGCTC

Downstream 100 bases:

>100_bases
AAGAGGTGGACTCGCCAGATCGTCATGGCCGACCCACGACGCGCCGCTTGACGAACGCTCGATGGTTCAGCAAAGCGTCA
GACGGCTGCGCTCTGGATTC

Product: biuret hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MDDTASVALARQNLAALGIPVSDEDLEKAHAAGFFSTAQVFQVMVQRDSEVDDLPDFLTDRLPAPRSWAATGEESPSAAP
EILELARALRNGETSSRALVERALEQITRRDQELNAFQLLLPEQALADAEARDAELARGIDRGPLHGLPVALKDLIDLAG
TPTTAGSAILANAVASRDATVVQRLRAAGAVIVGKTRLPEFAYSGASNNPHYGPVPNPRDRRRDSGGSSSGSAAAVAAGL
VVMAVGSDTGGSIRIPAAYCGIVGLKPTFGRVSARGVYPLAWSLDHIGPLTRTVTDAAVSLEAMAGPDEDDPRTRAVPLP
DLVSATQVERLELRVGVVRSTGGDQPLAAPDAVAACERAVAALADAGADLIPLVLPELETLRIVSAAIAQLEAAALHAPT
ARERWTHYGEFFRLRLLGCFAYPCWGYVAAQRLAKKARARVTSLLEEHDIALLALPTTPTTAPPLGQWTARASWLTAPFN
LLGWPALSVPAGLGDDGLPIGLQLVARPWREDLVIAAGALVERAARSSRGSN

Sequences:

>Translated_532_residues
MDDTASVALARQNLAALGIPVSDEDLEKAHAAGFFSTAQVFQVMVQRDSEVDDLPDFLTDRLPAPRSWAATGEESPSAAP
EILELARALRNGETSSRALVERALEQITRRDQELNAFQLLLPEQALADAEARDAELARGIDRGPLHGLPVALKDLIDLAG
TPTTAGSAILANAVASRDATVVQRLRAAGAVIVGKTRLPEFAYSGASNNPHYGPVPNPRDRRRDSGGSSSGSAAAVAAGL
VVMAVGSDTGGSIRIPAAYCGIVGLKPTFGRVSARGVYPLAWSLDHIGPLTRTVTDAAVSLEAMAGPDEDDPRTRAVPLP
DLVSATQVERLELRVGVVRSTGGDQPLAAPDAVAACERAVAALADAGADLIPLVLPELETLRIVSAAIAQLEAAALHAPT
ARERWTHYGEFFRLRLLGCFAYPCWGYVAAQRLAKKARARVTSLLEEHDIALLALPTTPTTAPPLGQWTARASWLTAPFN
LLGWPALSVPAGLGDDGLPIGLQLVARPWREDLVIAAGALVERAARSSRGSN
>Mature_532_residues
MDDTASVALARQNLAALGIPVSDEDLEKAHAAGFFSTAQVFQVMVQRDSEVDDLPDFLTDRLPAPRSWAATGEESPSAAP
EILELARALRNGETSSRALVERALEQITRRDQELNAFQLLLPEQALADAEARDAELARGIDRGPLHGLPVALKDLIDLAG
TPTTAGSAILANAVASRDATVVQRLRAAGAVIVGKTRLPEFAYSGASNNPHYGPVPNPRDRRRDSGGSSSGSAAAVAAGL
VVMAVGSDTGGSIRIPAAYCGIVGLKPTFGRVSARGVYPLAWSLDHIGPLTRTVTDAAVSLEAMAGPDEDDPRTRAVPLP
DLVSATQVERLELRVGVVRSTGGDQPLAAPDAVAACERAVAALADAGADLIPLVLPELETLRIVSAAIAQLEAAALHAPT
ARERWTHYGEFFRLRLLGCFAYPCWGYVAAQRLAKKARARVTSLLEEHDIALLALPTTPTTAPPLGQWTARASWLTAPFN
LLGWPALSVPAGLGDDGLPIGLQLVARPWREDLVIAAGALVERAARSSRGSN

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=506, Percent_Identity=29.6442687747036, Blast_Score=184, Evalue=2e-46,
Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=27.8688524590164, Blast_Score=150, Evalue=3e-36,
Organism=Homo sapiens, GI166795287, Length=485, Percent_Identity=27.0103092783505, Blast_Score=99, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17543272, Length=404, Percent_Identity=31.4356435643564, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17537465, Length=485, Percent_Identity=28.0412371134021, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=308, Percent_Identity=30.5194805194805, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17538254, Length=390, Percent_Identity=28.7179487179487, Blast_Score=105, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI71990152, Length=314, Percent_Identity=28.6624203821656, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17538252, Length=245, Percent_Identity=30.6122448979592, Blast_Score=99, Evalue=7e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=326, Percent_Identity=30.6748466257669, Blast_Score=95, Evalue=7e-20,
Organism=Caenorhabditis elegans, GI17556278, Length=326, Percent_Identity=30.6748466257669, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6323950, Length=418, Percent_Identity=29.4258373205742, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6319685, Length=402, Percent_Identity=32.5870646766169, Blast_Score=122, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6320448, Length=238, Percent_Identity=29.4117647058824, Blast_Score=83, Evalue=9e-17,
Organism=Drosophila melanogaster, GI24648113, Length=488, Percent_Identity=33.8114754098361, Blast_Score=176, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24644968, Length=514, Percent_Identity=28.2101167315175, Blast_Score=153, Evalue=4e-37,
Organism=Drosophila melanogaster, GI161078093, Length=486, Percent_Identity=29.2181069958848, Blast_Score=141, Evalue=1e-33,
Organism=Drosophila melanogaster, GI21356731, Length=483, Percent_Identity=27.536231884058, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24648435, Length=479, Percent_Identity=26.3048016701461, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24648437, Length=479, Percent_Identity=26.3048016701461, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45550774, Length=479, Percent_Identity=26.3048016701461, Blast_Score=126, Evalue=4e-29,
Organism=Drosophila melanogaster, GI24652985, Length=505, Percent_Identity=28.9108910891089, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI19922090, Length=505, Percent_Identity=28.9108910891089, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24652981, Length=505, Percent_Identity=28.9108910891089, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24652983, Length=505, Percent_Identity=28.9108910891089, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24648441, Length=408, Percent_Identity=26.7156862745098, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648439, Length=408, Percent_Identity=26.7156862745098, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 55959; Mature: 55959

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDDTASVALARQNLAALGIPVSDEDLEKAHAAGFFSTAQVFQVMVQRDSEVDDLPDFLTD
CCCHHHHHHHHHCHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHH
RLPAPRSWAATGEESPSAAPEILELARALRNGETSSRALVERALEQITRRDQELNAFQLL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
LPEQALADAEARDAELARGIDRGPLHGLPVALKDLIDLAGTPTTAGSAILANAVASRDAT
CCHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHH
VVQRLRAAGAVIVGKTRLPEFAYSGASNNPHYGPVPNPRDRRRDSGGSSSGSAAAVAAGL
HHHHHHHCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHCE
VVMAVGSDTGGSIRIPAAYCGIVGLKPTFGRVSARGVYPLAWSLDHIGPLTRTVTDAAVS
EEEEECCCCCCCEECCHHHHCCCCCCCCCCCCCCCCCCEEEECHHHCCHHHHHHHHHHHH
LEAMAGPDEDDPRTRAVPLPDLVSATQVERLELRVGVVRSTGGDQPLAAPDAVAACERAV
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHH
AALADAGADLIPLVLPELETLRIVSAAIAQLEAAALHAPTARERWTHYGEFFRLRLLGCF
HHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AYPCWGYVAAQRLAKKARARVTSLLEEHDIALLALPTTPTTAPPLGQWTARASWLTAPFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHCCHHCCCCHH
LLGWPALSVPAGLGDDGLPIGLQLVARPWREDLVIAAGALVERAARSSRGSN
HHCCCHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDDTASVALARQNLAALGIPVSDEDLEKAHAAGFFSTAQVFQVMVQRDSEVDDLPDFLTD
CCCHHHHHHHHHCHHHCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHCCCCHHHHHHHHHH
RLPAPRSWAATGEESPSAAPEILELARALRNGETSSRALVERALEQITRRDQELNAFQLL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCHHHHH
LPEQALADAEARDAELARGIDRGPLHGLPVALKDLIDLAGTPTTAGSAILANAVASRDAT
CCHHHHHCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCHHH
VVQRLRAAGAVIVGKTRLPEFAYSGASNNPHYGPVPNPRDRRRDSGGSSSGSAAAVAAGL
HHHHHHHCCEEEEECCCCCHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCCCHHHHHHCE
VVMAVGSDTGGSIRIPAAYCGIVGLKPTFGRVSARGVYPLAWSLDHIGPLTRTVTDAAVS
EEEEECCCCCCCEECCHHHHCCCCCCCCCCCCCCCCCCEEEECHHHCCHHHHHHHHHHHH
LEAMAGPDEDDPRTRAVPLPDLVSATQVERLELRVGVVRSTGGDQPLAAPDAVAACERAV
HHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCHHHHHHHHHH
AALADAGADLIPLVLPELETLRIVSAAIAQLEAAALHAPTARERWTHYGEFFRLRLLGCF
HHHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AYPCWGYVAAQRLAKKARARVTSLLEEHDIALLALPTTPTTAPPLGQWTARASWLTAPFN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCHHHCCHHCCCCHH
LLGWPALSVPAGLGDDGLPIGLQLVARPWREDLVIAAGALVERAARSSRGSN
HHCCCHHCCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA