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The map label for this gene is pcaJ [H]

Identifier: 221632331

GI number: 221632331

Start: 305169

End: 305861

Strand: Reverse

Name: pcaJ [H]

Synonym: trd_0302

Alternate gene names: 221632331

Gene position: 305861-305169 (Counterclockwise)

Preceding gene: 221632332

Following gene: 221632330

Centisome position: 15.27

GC content: 60.75

Gene sequence:

>693_bases
ATGCCGCTGACACGCGAGGGAATTGCCTACCGTATCGCCCATGATCTCCCCGACGGCAGCTTCGTCAACCTCGGCTTCGG
TATGCCGTCACTCGTTACGCACTTTTTGCCACTCGGGAAAACCGTGCTCATTCATAGCGAGAACGGCATTCTCGGTGCCG
GCCCCAAGGCACCCCCGGGTCAAGAAGATCCTGATCTCGTCAACGCTAACGGCGACTTCGTCACGCTCGTTCCGGGTGCG
AGTCTTTTCGACCATGCACTGTCGTTCACGATTGTTCGAGGAGGTCACTTGACGCATGCCGTGCTCGGTGCTCTTCAAGT
CTCGCGCCGGGGCGATCTGGCGAACTGGCGCATCCCTGGTGCTCGTGTTCCAGGGGTCGGCGGCGCCATGGACATCGCCG
TCGGAGCACAACGTGTCTGGGTAGCGATGACACATACCACCGATCACGGCGAGCCGAAGATCGTTGAAGAGTGCACCTAT
CCGCTGACTGCGCCGCGTTGTGTCCAGCGAATCTACACGGATCTCGCTGTTCTTTCCGTCGTGGGCGGCGAACTCCTCCT
CGAGGAAGTCGCACCGGGCGTCACGGTCCAAGACGTTACCGCGCGCACCGGTGCATCGCTGACCATTCCGTCTCGCGTGC
CGACGATGGACATTCCAGCTGAATTGGCCGGTATCCAACTGGTGACGGTCTGA

Upstream 100 bases:

>100_bases
AAATCGAAACACCGTGTCTTTTTGTCGACCGAGTCGTTGAGGTGCCGCGTGACTGGTTCCGACACGCAACATCCGCTGAA
CGACGGTGAGGTGGTACTCG

Downstream 100 bases:

>100_bases
TCGCTCGTGCAGAACGACAGAGGTCGATAAGGCGAAAGGAGGCCGTGGAAGAACGATGAGCGCGTCACCAACGGTGCTTC
GAGCAATCGATCGCGCACTC

Product: 3-oxoadipate CoA-succinyl transferase subunit beta

Products: NA

Alternate protein names: Beta-ketoadipate:succinyl-CoA transferase subunit B [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MPLTREGIAYRIAHDLPDGSFVNLGFGMPSLVTHFLPLGKTVLIHSENGILGAGPKAPPGQEDPDLVNANGDFVTLVPGA
SLFDHALSFTIVRGGHLTHAVLGALQVSRRGDLANWRIPGARVPGVGGAMDIAVGAQRVWVAMTHTTDHGEPKIVEECTY
PLTAPRCVQRIYTDLAVLSVVGGELLLEEVAPGVTVQDVTARTGASLTIPSRVPTMDIPAELAGIQLVTV

Sequences:

>Translated_230_residues
MPLTREGIAYRIAHDLPDGSFVNLGFGMPSLVTHFLPLGKTVLIHSENGILGAGPKAPPGQEDPDLVNANGDFVTLVPGA
SLFDHALSFTIVRGGHLTHAVLGALQVSRRGDLANWRIPGARVPGVGGAMDIAVGAQRVWVAMTHTTDHGEPKIVEECTY
PLTAPRCVQRIYTDLAVLSVVGGELLLEEVAPGVTVQDVTARTGASLTIPSRVPTMDIPAELAGIQLVTV
>Mature_229_residues
PLTREGIAYRIAHDLPDGSFVNLGFGMPSLVTHFLPLGKTVLIHSENGILGAGPKAPPGQEDPDLVNANGDFVTLVPGAS
LFDHALSFTIVRGGHLTHAVLGALQVSRRGDLANWRIPGARVPGVGGAMDIAVGAQRVWVAMTHTTDHGEPKIVEECTYP
LTAPRCVQRIYTDLAVLSVVGGELLLEEVAPGVTVQDVTARTGASLTIPSRVPTMDIPAELAGIQLVTV

Specific function: SHORT-CHAIN FATTY ACIDS METABOLISM. [C]

COG id: COG2057

COG function: function code I; Acyl CoA:acetate/3-ketoacid CoA transferase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 3-oxoacid CoA-transferase subunit B family [H]

Homologues:

Organism=Homo sapiens, GI11545841, Length=207, Percent_Identity=46.8599033816425, Blast_Score=184, Evalue=4e-47,
Organism=Homo sapiens, GI4557817, Length=217, Percent_Identity=45.1612903225806, Blast_Score=180, Evalue=1e-45,
Organism=Escherichia coli, GI1788552, Length=211, Percent_Identity=44.0758293838863, Blast_Score=189, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17531517, Length=215, Percent_Identity=45.5813953488372, Blast_Score=179, Evalue=1e-45,
Organism=Drosophila melanogaster, GI21358325, Length=214, Percent_Identity=46.2616822429907, Blast_Score=187, Evalue=4e-48,
Organism=Drosophila melanogaster, GI24655794, Length=214, Percent_Identity=46.2616822429907, Blast_Score=187, Evalue=5e-48,

Paralogues:

None

Copy number: 369 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012791
- InterPro:   IPR004165
- InterPro:   IPR004164 [H]

Pfam domain/function: PF01144 CoA_trans [H]

EC number: =2.8.3.6 [H]

Molecular weight: Translated: 24156; Mature: 24025

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLTREGIAYRIAHDLPDGSFVNLGFGMPSLVTHFLPLGKTVLIHSENGILGAGPKAPPG
CCCCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHCCCCEEEEECCCCEEECCCCCCCC
QEDPDLVNANGDFVTLVPGASLFDHALSFTIVRGGHLTHAVLGALQVSRRGDLANWRIPG
CCCCCEEECCCCEEEEECCCHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCCCEECCC
ARVPGVGGAMDIAVGAQRVWVAMTHTTDHGEPKIVEECTYPLTAPRCVQRIYTDLAVLSV
CCCCCCCCCEEEHCCCEEEEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
VGGELLLEEVAPGVTVQDVTARTGASLTIPSRVPTMDIPAELAGIQLVTV
HHHHHHHHHHCCCCEEEEEHHCCCCEEEECCCCCCCCCCHHHCCEEEEEC
>Mature Secondary Structure 
PLTREGIAYRIAHDLPDGSFVNLGFGMPSLVTHFLPLGKTVLIHSENGILGAGPKAPPG
CCCCCCCEEEEEECCCCCCEEEECCCCHHHHHHHHCCCCEEEEECCCCEEECCCCCCCC
QEDPDLVNANGDFVTLVPGASLFDHALSFTIVRGGHLTHAVLGALQVSRRGDLANWRIPG
CCCCCEEECCCCEEEEECCCHHHHHHHEEEEEECCCHHHHHHHHHHHHCCCCCCCEECCC
ARVPGVGGAMDIAVGAQRVWVAMTHTTDHGEPKIVEECTYPLTAPRCVQRIYTDLAVLSV
CCCCCCCCCEEEHCCCEEEEEEEEECCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHH
VGGELLLEEVAPGVTVQDVTARTGASLTIPSRVPTMDIPAELAGIQLVTV
HHHHHHHHHHCCCCEEEEEHHCCCCEEEECCCCCCCCCCHHHCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8063101; 8125318 [H]