BacMap

Definition	Thermomicrobium roseum DSM 5159 chromosome, complete genome.
Accession	NC_011959
Length	2,003,006

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The map label for this gene is yngF [H]

Identifier: 221632319

GI number: 221632319

Start: 291565

End: 292383

Strand: Reverse

Name: yngF [H]

Synonym: trd_0285

Alternate gene names: 221632319

Gene position: 292383-291565 (Counterclockwise)

Preceding gene: 221632324

Following gene: 221632317

Centisome position: 14.6

GC content: 67.89

Gene sequence:

>819_bases
ATGAAGGGATCCTCGCTCGATCGACCGGAGAGGGTAGACGCGATGGAGCAACCGGTCCTGCTCGTCGCACATCATGCTGG
GATCGTGACGCTCACGCTCAATCGGCCGACTGTCCGCAACGCGCTGGATCTCGCTCTCGCGCGAGCGATCGCGACGACCG
CACAGGAGCTCGCCCAGGATGCGAGCCTGCGCCTCGTCGTCTTGCGCGGTACCCCGCCCGCCTTCTGTGCCGGAGCGGAC
CTGACCGAGCGTTCGAAGATGTCCCCACGCGAACGCACCGCTCACACCGATGCCATCGCCGCCGCGGTCGAGGCGATCGC
TGAGCTACCGGTTCCGACGATCGCCGCGATCAGCGGCGCCTGTCTGGCAGGCGGGGCTGAACTCGCCCTCGCCTGTGACC
TCCGCTTCGCGGATACCACCGCCCGCATCGGCTTCCCGGAAGTGCGCCGCGGCATCTTCCCCGGGGCAGGCGGTGTCCTG
CGCCTGCCCCAACTCGTCGGCCGCGGGCGAGCAGCTGATCTCCTCTTTTCCGGCCGAACACTCGATGCGCAGGAAGCGTT
CCGCATCGGGCTCATCGACCAGCTCTGCGAGCCGGGTCATCTCGACACCATGCTCGACCACTGGATCACCGAACTCCGGC
AAGCGGCTCCCGGCGCAGTGCGCGCGGTCAAAGCTGCTCTCCAGCGGGTACTCACCACCGACGCGGTCACGCTCGCCGAT
ATCCGGTCCCTCCGCCGCGCACTCGACGAAAGTCCGGAGTACGAGGAAGGACTCCGGGCCTTCGCCGAACGACGCCCACC
GAGTTGGGCGAACGGTTAA

Upstream 100 bases:

>100_bases
CGCGGCTCTGGTACGCAGAGACCAGCGGCCGACTCTGGTCAACACTCGCAGTGAGCAGCGTGTGCCATACTCGGTCGTGC
GGGGGAAGCTGCCACCGGTT

Downstream 100 bases:

>100_bases
TTCGTTTCGACCGTAAACGTCGTCTCGAGGTCATTGACGACCAGGGTGTAGGTTCCTGGTTCCAACTCGCCGAGATCGAC
AGAGGTCGTGAACGGTCGCA

Product: enoyl-CoA hydratase/isomerase family protein

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MKGSSLDRPERVDAMEQPVLLVAHHAGIVTLTLNRPTVRNALDLALARAIATTAQELAQDASLRLVVLRGTPPAFCAGAD
LTERSKMSPRERTAHTDAIAAAVEAIAELPVPTIAAISGACLAGGAELALACDLRFADTTARIGFPEVRRGIFPGAGGVL
RLPQLVGRGRAADLLFSGRTLDAQEAFRIGLIDQLCEPGHLDTMLDHWITELRQAAPGAVRAVKAALQRVLTTDAVTLAD
IRSLRRALDESPEYEEGLRAFAERRPPSWANG

Sequences:

>Translated_272_residues
MKGSSLDRPERVDAMEQPVLLVAHHAGIVTLTLNRPTVRNALDLALARAIATTAQELAQDASLRLVVLRGTPPAFCAGAD
LTERSKMSPRERTAHTDAIAAAVEAIAELPVPTIAAISGACLAGGAELALACDLRFADTTARIGFPEVRRGIFPGAGGVL
RLPQLVGRGRAADLLFSGRTLDAQEAFRIGLIDQLCEPGHLDTMLDHWITELRQAAPGAVRAVKAALQRVLTTDAVTLAD
IRSLRRALDESPEYEEGLRAFAERRPPSWANG
>Mature_272_residues
MKGSSLDRPERVDAMEQPVLLVAHHAGIVTLTLNRPTVRNALDLALARAIATTAQELAQDASLRLVVLRGTPPAFCAGAD
LTERSKMSPRERTAHTDAIAAAVEAIAELPVPTIAAISGACLAGGAELALACDLRFADTTARIGFPEVRRGIFPGAGGVL
RLPQLVGRGRAADLLFSGRTLDAQEAFRIGLIDQLCEPGHLDTMLDHWITELRQAAPGAVRAVKAALQRVLTTDAVTLAD
IRSLRRALDESPEYEEGLRAFAERRPPSWANG

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI4502327, Length=256, Percent_Identity=39.453125, Blast_Score=141, Evalue=5e-34,
Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=28.9795918367347, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI20127408, Length=176, Percent_Identity=35.2272727272727, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI68989263, Length=241, Percent_Identity=31.1203319502075, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI157694516, Length=211, Percent_Identity=27.4881516587678, Blast_Score=96, Evalue=2e-20,
Organism=Homo sapiens, GI213417737, Length=205, Percent_Identity=27.8048780487805, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI31542718, Length=254, Percent_Identity=27.1653543307087, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI70995211, Length=215, Percent_Identity=30.2325581395349, Blast_Score=90, Evalue=3e-18,
Organism=Homo sapiens, GI157694520, Length=166, Percent_Identity=27.710843373494, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI221136756, Length=214, Percent_Identity=28.5046728971963, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI221136753, Length=214, Percent_Identity=28.5046728971963, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI221307494, Length=214, Percent_Identity=28.5046728971963, Blast_Score=78, Evalue=7e-15,
Organism=Homo sapiens, GI37594471, Length=193, Percent_Identity=29.0155440414508, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI37594469, Length=197, Percent_Identity=28.9340101522843, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI25453481, Length=211, Percent_Identity=26.0663507109005, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI4757966, Length=211, Percent_Identity=26.0663507109005, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51538573, Length=211, Percent_Identity=26.0663507109005, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI51538581, Length=211, Percent_Identity=26.0663507109005, Blast_Score=75, Evalue=7e-14,
Organism=Homo sapiens, GI62530384, Length=244, Percent_Identity=26.2295081967213, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI4757968, Length=181, Percent_Identity=25.414364640884, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI49355787, Length=181, Percent_Identity=25.414364640884, Blast_Score=70, Evalue=2e-12,
Organism=Homo sapiens, GI261878539, Length=144, Percent_Identity=32.6388888888889, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI116235487, Length=248, Percent_Identity=25.8064516129032, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1787659, Length=255, Percent_Identity=34.1176470588235, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1790281, Length=174, Percent_Identity=36.7816091954023, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=28.1124497991968, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1788682, Length=177, Percent_Identity=36.1581920903955, Blast_Score=98, Evalue=5e-22,
Organism=Escherichia coli, GI221142681, Length=258, Percent_Identity=29.0697674418605, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1787660, Length=256, Percent_Identity=28.90625, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI87082183, Length=214, Percent_Identity=26.1682242990654, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17540714, Length=187, Percent_Identity=35.2941176470588, Blast_Score=127, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI25145438, Length=244, Percent_Identity=31.1475409836066, Blast_Score=119, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17554946, Length=250, Percent_Identity=31.2, Blast_Score=116, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17560910, Length=173, Percent_Identity=33.5260115606936, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17558304, Length=186, Percent_Identity=33.8709677419355, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI25144276, Length=186, Percent_Identity=34.4086021505376, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17508951, Length=186, Percent_Identity=34.4086021505376, Blast_Score=91, Evalue=5e-19,
Organism=Caenorhabditis elegans, GI17508953, Length=186, Percent_Identity=34.4086021505376, Blast_Score=91, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17534483, Length=183, Percent_Identity=30.0546448087432, Blast_Score=88, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17536985, Length=221, Percent_Identity=27.6018099547511, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25144160, Length=221, Percent_Identity=28.0542986425339, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25144157, Length=221, Percent_Identity=28.0542986425339, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI25141363, Length=201, Percent_Identity=26.3681592039801, Blast_Score=77, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6320241, Length=199, Percent_Identity=26.6331658291457, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=34.2105263157895, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI20129971, Length=245, Percent_Identity=31.8367346938775, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24653477, Length=245, Percent_Identity=31.8367346938775, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI21357171, Length=184, Percent_Identity=32.6086956521739, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24650670, Length=184, Percent_Identity=33.695652173913, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=24.3243243243243, Blast_Score=91, Evalue=8e-19,
Organism=Drosophila melanogaster, GI24583077, Length=177, Percent_Identity=32.7683615819209, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24583079, Length=177, Percent_Identity=32.7683615819209, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI19921000, Length=177, Percent_Identity=32.7683615819209, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI28571729, Length=176, Percent_Identity=31.25, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28571730, Length=176, Percent_Identity=31.25, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921018, Length=175, Percent_Identity=32, Blast_Score=75, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24583165, Length=177, Percent_Identity=32.7683615819209, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24654903, Length=256, Percent_Identity=21.875, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920382, Length=183, Percent_Identity=28.4153005464481, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 29000; Mature: 29000

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGSSLDRPERVDAMEQPVLLVAHHAGIVTLTLNRPTVRNALDLALARAIATTAQELAQD
CCCCCCCCCHHHHHHHCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
ASLRLVVLRGTPPAFCAGADLTERSKMSPRERTAHTDAIAAAVEAIAELPVPTIAAISGA
CCEEEEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
CLAGGAELALACDLRFADTTARIGFPEVRRGIFPGAGGVLRLPQLVGRGRAADLLFSGRT
HHCCCCCEEEEEECEECCCHHHCCCHHHHHCCCCCCCCHHHHHHHHCCCCHHHHEECCCC
LDAQEAFRIGLIDQLCEPGHLDTMLDHWITELRQAAPGAVRAVKAALQRVLTTDAVTLAD
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IRSLRRALDESPEYEEGLRAFAERRPPSWANG
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKGSSLDRPERVDAMEQPVLLVAHHAGIVTLTLNRPTVRNALDLALARAIATTAQELAQD
CCCCCCCCCHHHHHHHCCEEEEEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
ASLRLVVLRGTPPAFCAGADLTERSKMSPRERTAHTDAIAAAVEAIAELPVPTIAAISGA
CCEEEEEEECCCCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
CLAGGAELALACDLRFADTTARIGFPEVRRGIFPGAGGVLRLPQLVGRGRAADLLFSGRT
HHCCCCCEEEEEECEECCCHHHCCCHHHHHCCCCCCCCHHHHHHHHCCCCHHHHEECCCC
LDAQEAFRIGLIDQLCEPGHLDTMLDHWITELRQAAPGAVRAVKAALQRVLTTDAVTLAD
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
IRSLRRALDESPEYEEGLRAFAERRPPSWANG
HHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]