Click here to switch to the map view.

The map label for this gene is carA [H]

Identifier: 221632247

GI number: 221632247

Start: 222754

End: 223905

Strand: Reverse

Name: carA [H]

Synonym: trd_0213

Alternate gene names: 221632247

Gene position: 223905-222754 (Counterclockwise)

Preceding gene: 221632248

Following gene: 221632246

Centisome position: 11.18

GC content: 63.63

Gene sequence:

>1152_bases
ATGTCGATACAGCGTTCGCGGATCGGTGCACTCGTTCTGGAAGACGGGCGGATCTTTCCGGGTACTCCCTTCGGAGCACG
CCGCCCGGCTGAGGGCGAAGCGGTCTTCACCACTGTCATGACTGGTTATCAAGAGGTGGTCACTGACCCCTCTTTTTATG
GGCAACTCGTCTGCATGACATACCCACTCATCGGTAATTACGGTGTCTGTGACGAGGACGAACAATCCCGACGCCCGTGG
GCCGCCGGGCTGATCGTCCGCGAGTACTGCGACCGTCCGAGTCACTGGAAGGCGCGCGAGACGCTCGCCAGTTATCTGGA
GCGATGGGGCATACCAGGGCTGGCTCATGTCGATACGCGCGCTTTGACGCGCCACCTGCGCACGCACGGAACCATGCGTG
CGATCCTCGTTCCCGACCGCACGGGCCTGACCGACGCCGAACTCGTTGCCTGGGCGCAGCGAGCCTGGACGCCCGATCGA
GAAGATGTCGTCCCAGCCGTTCGCGGCGAGCCAGCGACCTTCGGGCCAGCGGATGCACCGCACATCGTCCTCATCGACTG
CGGCGTCAAGCGCAACATCATCGCCTCCCTCCTCCGCCGCGCTGTACGCCTCACCGTCCTTCCCTATGGGGTCGATGCAG
CGACCGTCCTCGCGCTGGAGCCGGACGGGATCGTCGTCTCACCTGGCCCCGGTGACCCGACCCGAGCCACCGATGCCATC
GAAACGGTCCGCGTTCTGGCCACCTCGGGGATACCGTTCTTCGGTATCTGCCTGGGTCATCAGCTCCTGGCCCTCGCAGC
CGGCGCTCGAACGATCAAGTTGACCTTCGGCCACCGTGGCGGCAACCACCCGGTCAAGGAGATCCCCACCGGCCGGGTGC
GTATAACGTCACAGAATCACGGCTACTGCGTGGCCCACGACAGCATACCCGAGCAGGATGGCTGGCAAGTGTGGATGATC
AATGTGAACGATGGAACAGTCGAAGGGCTCCGGCATCGCTCGCTTCCTGTCCTGACTGTCCAGTTTCATCCCGAGGGATC
ACCCGGACCACAAGATAGCCAAGATCTCTTCGACGCTTTCGTCGAACTCGTCCAGCAGCGGTGGCGAGCACGCACAGCGC
GGCCGATCGTGCTCGCTGGGGAAGGGGCATGA

Upstream 100 bases:

>100_bases
ACGCGCGCGACATTGCGCTCGCGCAGTGCCAACACACCGCTCCTCGGACTGACCCTGCGTGGGCGCGTCCGACTCACGAT
CGTTGGAGGGAAGGTAGCCT

Downstream 100 bases:

>100_bases
GGATGGCACGGCAGCCGTTGGTTTCCAGCGTCCTCGTCATCGGATCGGGTCCGATCGTCATCGGGCAGGCCGCGGAGTTC
GACTACTCGGGAAGTCAAGC

Product: carbamoyl-phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 383; Mature: 382

Protein sequence:

>383_residues
MSIQRSRIGALVLEDGRIFPGTPFGARRPAEGEAVFTTVMTGYQEVVTDPSFYGQLVCMTYPLIGNYGVCDEDEQSRRPW
AAGLIVREYCDRPSHWKARETLASYLERWGIPGLAHVDTRALTRHLRTHGTMRAILVPDRTGLTDAELVAWAQRAWTPDR
EDVVPAVRGEPATFGPADAPHIVLIDCGVKRNIIASLLRRAVRLTVLPYGVDAATVLALEPDGIVVSPGPGDPTRATDAI
ETVRVLATSGIPFFGICLGHQLLALAAGARTIKLTFGHRGGNHPVKEIPTGRVRITSQNHGYCVAHDSIPEQDGWQVWMI
NVNDGTVEGLRHRSLPVLTVQFHPEGSPGPQDSQDLFDAFVELVQQRWRARTARPIVLAGEGA

Sequences:

>Translated_383_residues
MSIQRSRIGALVLEDGRIFPGTPFGARRPAEGEAVFTTVMTGYQEVVTDPSFYGQLVCMTYPLIGNYGVCDEDEQSRRPW
AAGLIVREYCDRPSHWKARETLASYLERWGIPGLAHVDTRALTRHLRTHGTMRAILVPDRTGLTDAELVAWAQRAWTPDR
EDVVPAVRGEPATFGPADAPHIVLIDCGVKRNIIASLLRRAVRLTVLPYGVDAATVLALEPDGIVVSPGPGDPTRATDAI
ETVRVLATSGIPFFGICLGHQLLALAAGARTIKLTFGHRGGNHPVKEIPTGRVRITSQNHGYCVAHDSIPEQDGWQVWMI
NVNDGTVEGLRHRSLPVLTVQFHPEGSPGPQDSQDLFDAFVELVQQRWRARTARPIVLAGEGA
>Mature_382_residues
SIQRSRIGALVLEDGRIFPGTPFGARRPAEGEAVFTTVMTGYQEVVTDPSFYGQLVCMTYPLIGNYGVCDEDEQSRRPWA
AGLIVREYCDRPSHWKARETLASYLERWGIPGLAHVDTRALTRHLRTHGTMRAILVPDRTGLTDAELVAWAQRAWTPDRE
DVVPAVRGEPATFGPADAPHIVLIDCGVKRNIIASLLRRAVRLTVLPYGVDAATVLALEPDGIVVSPGPGDPTRATDAIE
TVRVLATSGIPFFGICLGHQLLALAAGARTIKLTFGHRGGNHPVKEIPTGRVRITSQNHGYCVAHDSIPEQDGWQVWMIN
VNDGTVEGLRHRSLPVLTVQFHPEGSPGPQDSQDLFDAFVELVQQRWRARTARPIVLAGEGA

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=371, Percent_Identity=44.4743935309973, Blast_Score=281, Evalue=5e-76,
Organism=Homo sapiens, GI21361331, Length=375, Percent_Identity=35.4666666666667, Blast_Score=235, Evalue=6e-62,
Organism=Homo sapiens, GI169790915, Length=375, Percent_Identity=35.4666666666667, Blast_Score=235, Evalue=6e-62,
Organism=Escherichia coli, GI1786215, Length=386, Percent_Identity=41.9689119170984, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1789760, Length=149, Percent_Identity=31.5436241610738, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI193204318, Length=372, Percent_Identity=41.3978494623656, Blast_Score=269, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6322331, Length=409, Percent_Identity=40.0977995110024, Blast_Score=284, Evalue=1e-77,
Organism=Saccharomyces cerevisiae, GI6324878, Length=385, Percent_Identity=39.7402597402597, Blast_Score=264, Evalue=2e-71,
Organism=Drosophila melanogaster, GI45555749, Length=380, Percent_Identity=41.3157894736842, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24642586, Length=385, Percent_Identity=40.2597402597403, Blast_Score=264, Evalue=8e-71,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41902; Mature: 41771

Theoretical pI: Translated: 6.95; Mature: 6.95

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIQRSRIGALVLEDGRIFPGTPFGARRPAEGEAVFTTVMTGYQEVVTDPSFYGQLVCMT
CCCCCCCCCEEEEECCEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCHHHCEEEEEE
YPLIGNYGVCDEDEQSRRPWAAGLIVREYCDRPSHWKARETLASYLERWGIPGLAHVDTR
ECCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
ALTRHLRTHGTMRAILVPDRTGLTDAELVAWAQRAWTPDREDVVPAVRGEPATFGPADAP
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCC
HIVLIDCGVKRNIIASLLRRAVRLTVLPYGVDAATVLALEPDGIVVSPGPGDPTRATDAI
EEEEEECCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCEEECCCCCCCCHHHHHH
ETVRVLATSGIPFFGICLGHQLLALAAGARTIKLTFGHRGGNHPVKEIPTGRVRITSQNH
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCEEEEEECCC
GYCVAHDSIPEQDGWQVWMINVNDGTVEGLRHRSLPVLTVQFHPEGSPGPQDSQDLFDAF
CEEEEECCCCCCCCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHH
VELVQQRWRARTARPIVLAGEGA
HHHHHHHHHHHCCCEEEEECCCC
>Mature Secondary Structure 
SIQRSRIGALVLEDGRIFPGTPFGARRPAEGEAVFTTVMTGYQEVVTDPSFYGQLVCMT
CCCCCCCCEEEEECCEECCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCHHHCEEEEEE
YPLIGNYGVCDEDEQSRRPWAAGLIVREYCDRPSHWKARETLASYLERWGIPGLAHVDTR
ECCCCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHH
ALTRHLRTHGTMRAILVPDRTGLTDAELVAWAQRAWTPDREDVVPAVRGEPATFGPADAP
HHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHCCCCCCCCCCCCCCCCCC
HIVLIDCGVKRNIIASLLRRAVRLTVLPYGVDAATVLALEPDGIVVSPGPGDPTRATDAI
EEEEEECCCCHHHHHHHHHHHHEEEEEECCCCEEEEEEECCCCEEECCCCCCCCHHHHHH
ETVRVLATSGIPFFGICLGHQLLALAAGARTIKLTFGHRGGNHPVKEIPTGRVRITSQNH
HHHHHHHHCCCCHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHCCCCEEEEEECCC
GYCVAHDSIPEQDGWQVWMINVNDGTVEGLRHRSLPVLTVQFHPEGSPGPQDSQDLFDAF
CEEEEECCCCCCCCCEEEEEECCCCCHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHH
VELVQQRWRARTARPIVLAGEGA
HHHHHHHHHHHCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA