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The map label for this gene is torZ [C]

Identifier: 22127058

GI number: 22127058

Start: 3501793

End: 3503919

Strand: Reverse

Name: torZ [C]

Synonym: y3182

Alternate gene names: 22127058

Gene position: 3503919-3501793 (Counterclockwise)

Preceding gene: 22127060

Following gene: 161484766

Centisome position: 76.16

GC content: 51.81

Gene sequence:

>2127_bases
ATGGTTTACTCCGATAAACGTATCCGTAAACCCGCCATCCGCCGTTCTTGGTTAAAGCGGCGGGAGAAGAGTGACCGGAC
TCTACGCGGGCAGGAGGATTTTGTTGAAGTCGACTGGGATGTGGCGCTGGATTTAGTGGCCGAAGAGAATCGCCGGGTTA
GGGACCAATACGGGCCTGCTGGGTTATTTACCGGCTCTTATGGCTGGTCTTCTGCGGGGCGTTTACATCATGCCCGCTCA
TTAGTTCGGCGTTTCTATTTCAGTGGCGGTGGCGGTGTCGATCAATTAGGTAATTACAGTTGGGGGGCTGCACAATTTTT
CCTGCCTTATGTCATTGGGACCTTTAGCCCATTGACCGGGCGGGTGACGTCATGGCCCAGTGTGGTGGAACACTGTGAAT
TGTTCATTGCTTTTGGTGGGCTGGCACTGAAAAATGCGCAAGTCTCCTCCGGTGGGTCGGCTGAACATTCCCTTAAACTC
TGGCTAGAGAAACTGGCACAAAAAGGGACGCCAGTCATTAATATCAGCCCGATGCGTGATGATTGCCCCGCATTTGTTAA
CGCGGAATGGATACCCATTCGTCCGAATACAGACGTCGCGCTGATGTTGGCGTTGGCTTATGAAATTCAGCGCCTTGATG
GTCATGATGAGGCATTCCTTGCAACCCATTGTGTGGGTTATGGTCAATTGGCTGATTATATTCGTGGGGCCAATGACGGG
ATAGCGAAAACTCCAGACTGGGCGAGCAGCATTACCGGTATTCCTGCGGCCAGAATCGCACAGTTAGCGCAACAACTTAT
CGGTGTGCGCAGTTTTATGACCTGTTCGTATTCGGTGCAACGTGCGCATCGTGGTGAGCAACCGTATTGGATGGTGATTG
CATTATCAGCCATGTTGGGGCAAGTCGGGCTACCGGGTGGGGGTTTTTCATTCGGTCATGGTTCGATGAATGGTGTGGGT
AACCCGCGCATTGATACACCGGGGCCAGCCATGCCAGTGGGTAAGAACCCCGCCGGATTGGCTATTCCTGTGGCACGGAT
TTGCGACATGTTGTTACAACCGGGGCAACCATATCAATTTCAGGGTGAAACACATACCTACCCGGATATTCATTTGGTGC
ACTGGGCGGGTGGGAACCCGTTCCATCATCATCAGCAGTTGAATCGGTTGGTTGAGGGGTGGCAAAAACCGGATACCGTG
ATCGTACAGGACATTTGGTGGACGCCCGTAGCAAAAATGGCCGATATCGTCCTGCCAGTCACCAGTTCACTGGAGCGTAA
TGATATCGGTGGATCATCCAGGGATCGCTATGTCTTGGCGATGCATCAGGCGATTGCACCACAGCATCAGGCGCGCCACG
ATTTTGATATTTTCGCCGACTTGGCTGACCGATTGGGCTACCGTGAAACCTTTACCGAGGGTCGTGATGAAATGGCGTGG
ATCAGGCATATTTATCAGGAGTGTGGTGCGGCACAGCGCTCTCATGATGTTGAATGGCCAGATTTTGAGACTTTTTGGCA
ACAAGGGTATGTCGGTTTACCTGCTCCCTCCAAAGCATTTGTGTTCTTTGATTCTTTCCGTGAAGACCCACAGGCTAATC
CATTACAGACCCCGAGTGGCAAAATTGAATTATTTAGTGAAAAAATTGCCAGCTACCAATACAACGATTTTGCACCTCAT
GCAGAGTGGAAACCTCCGGTCGAATGGCTGGGCGCTCCGCTGGCTGAACAGTGGCCGCTACATTTAATCTCTATTCAACC
GGGAGATCGCTTACACAGTCAGCTCGATCCCGCCCCATTGGCTCAGAGCAATAAAACCGCAGGTCGTGAAACGCTGTATA
TGCATCCGCTGGATGCGGCCAGCCGTGATATTACCGAAGGTAGTCAGGTCCAGGTTAGCAACTTACGCGGTCACTGTCTG
GCGGGGGTCAGTATTACTGATGGGGTTACCCGTGGGGTGGTATTGATGGCAACCGGAGCCTGGTTTGATCCTGGTTTTGG
GGGCACGCAGCATAAAATTGAACAATCCGGTAACCCAAATGTATTGACGCTGGATATTGGGACATCTCGATTGACTCAAG
GCCCAAATGCGATGAGCTGTTTGGTCGAGGTGGCGCTTTATGAGTAG

Upstream 100 bases:

>100_bases
GGGCGCTTATACCGCAGTTGTTGAAGATGGCAAGCTGATCCGCTGCGAGCCATTTGCCGATGATCCCGATCCCTCGCCGC
TACTGAACTCAATTGTTCCG

Downstream 100 bases:

>100_bases
ATTATCTTTCGTTTGCCGCCTTCCTGTAGCTCGAATTATTTAGGGTCTATTCGCCAAACAGCCACTTGGGAATGTATTTC
TCTGGTGGCTGAAATGCGAT

Product: reductase

Products: Biotin [C]

Alternate protein names: BDS reductase; BSO reductase [H]

Number of amino acids: Translated: 708; Mature: 708

Protein sequence:

>708_residues
MVYSDKRIRKPAIRRSWLKRREKSDRTLRGQEDFVEVDWDVALDLVAEENRRVRDQYGPAGLFTGSYGWSSAGRLHHARS
LVRRFYFSGGGGVDQLGNYSWGAAQFFLPYVIGTFSPLTGRVTSWPSVVEHCELFIAFGGLALKNAQVSSGGSAEHSLKL
WLEKLAQKGTPVINISPMRDDCPAFVNAEWIPIRPNTDVALMLALAYEIQRLDGHDEAFLATHCVGYGQLADYIRGANDG
IAKTPDWASSITGIPAARIAQLAQQLIGVRSFMTCSYSVQRAHRGEQPYWMVIALSAMLGQVGLPGGGFSFGHGSMNGVG
NPRIDTPGPAMPVGKNPAGLAIPVARICDMLLQPGQPYQFQGETHTYPDIHLVHWAGGNPFHHHQQLNRLVEGWQKPDTV
IVQDIWWTPVAKMADIVLPVTSSLERNDIGGSSRDRYVLAMHQAIAPQHQARHDFDIFADLADRLGYRETFTEGRDEMAW
IRHIYQECGAAQRSHDVEWPDFETFWQQGYVGLPAPSKAFVFFDSFREDPQANPLQTPSGKIELFSEKIASYQYNDFAPH
AEWKPPVEWLGAPLAEQWPLHLISIQPGDRLHSQLDPAPLAQSNKTAGRETLYMHPLDAASRDITEGSQVQVSNLRGHCL
AGVSITDGVTRGVVLMATGAWFDPGFGGTQHKIEQSGNPNVLTLDIGTSRLTQGPNAMSCLVEVALYE

Sequences:

>Translated_708_residues
MVYSDKRIRKPAIRRSWLKRREKSDRTLRGQEDFVEVDWDVALDLVAEENRRVRDQYGPAGLFTGSYGWSSAGRLHHARS
LVRRFYFSGGGGVDQLGNYSWGAAQFFLPYVIGTFSPLTGRVTSWPSVVEHCELFIAFGGLALKNAQVSSGGSAEHSLKL
WLEKLAQKGTPVINISPMRDDCPAFVNAEWIPIRPNTDVALMLALAYEIQRLDGHDEAFLATHCVGYGQLADYIRGANDG
IAKTPDWASSITGIPAARIAQLAQQLIGVRSFMTCSYSVQRAHRGEQPYWMVIALSAMLGQVGLPGGGFSFGHGSMNGVG
NPRIDTPGPAMPVGKNPAGLAIPVARICDMLLQPGQPYQFQGETHTYPDIHLVHWAGGNPFHHHQQLNRLVEGWQKPDTV
IVQDIWWTPVAKMADIVLPVTSSLERNDIGGSSRDRYVLAMHQAIAPQHQARHDFDIFADLADRLGYRETFTEGRDEMAW
IRHIYQECGAAQRSHDVEWPDFETFWQQGYVGLPAPSKAFVFFDSFREDPQANPLQTPSGKIELFSEKIASYQYNDFAPH
AEWKPPVEWLGAPLAEQWPLHLISIQPGDRLHSQLDPAPLAQSNKTAGRETLYMHPLDAASRDITEGSQVQVSNLRGHCL
AGVSITDGVTRGVVLMATGAWFDPGFGGTQHKIEQSGNPNVLTLDIGTSRLTQGPNAMSCLVEVALYE
>Mature_708_residues
MVYSDKRIRKPAIRRSWLKRREKSDRTLRGQEDFVEVDWDVALDLVAEENRRVRDQYGPAGLFTGSYGWSSAGRLHHARS
LVRRFYFSGGGGVDQLGNYSWGAAQFFLPYVIGTFSPLTGRVTSWPSVVEHCELFIAFGGLALKNAQVSSGGSAEHSLKL
WLEKLAQKGTPVINISPMRDDCPAFVNAEWIPIRPNTDVALMLALAYEIQRLDGHDEAFLATHCVGYGQLADYIRGANDG
IAKTPDWASSITGIPAARIAQLAQQLIGVRSFMTCSYSVQRAHRGEQPYWMVIALSAMLGQVGLPGGGFSFGHGSMNGVG
NPRIDTPGPAMPVGKNPAGLAIPVARICDMLLQPGQPYQFQGETHTYPDIHLVHWAGGNPFHHHQQLNRLVEGWQKPDTV
IVQDIWWTPVAKMADIVLPVTSSLERNDIGGSSRDRYVLAMHQAIAPQHQARHDFDIFADLADRLGYRETFTEGRDEMAW
IRHIYQECGAAQRSHDVEWPDFETFWQQGYVGLPAPSKAFVFFDSFREDPQANPLQTPSGKIELFSEKIASYQYNDFAPH
AEWKPPVEWLGAPLAEQWPLHLISIQPGDRLHSQLDPAPLAQSNKTAGRETLYMHPLDAASRDITEGSQVQVSNLRGHCL
AGVSITDGVTRGVVLMATGAWFDPGFGGTQHKIEQSGNPNVLTLDIGTSRLTQGPNAMSCLVEVALYE

Specific function: This enzyme may serve as a scavenger, allowing the cell to utilize biotin sulfoxide as a biotin source. It reduces a spontaneous oxidation product of biotin, D-biotin D-sulfoxide (BSO or BDS), back to biotin [H]

COG id: COG0243

COG function: function code C; Anaerobic dehydrogenases, typically selenocysteine-containing

Gene ontology:

Cell location: Periplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic molybdopterin-containing oxidoreductase family [H]

Homologues:

Organism=Escherichia coli, GI87081994, Length=725, Percent_Identity=39.5862068965517, Blast_Score=534, Evalue=1e-153,
Organism=Escherichia coli, GI145693196, Length=719, Percent_Identity=40.7510431154381, Blast_Score=521, Evalue=1e-149,
Organism=Escherichia coli, GI1787231, Length=730, Percent_Identity=39.5890410958904, Blast_Score=489, Evalue=1e-139,
Organism=Escherichia coli, GI87081797, Length=713, Percent_Identity=30.8555399719495, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1787870, Length=748, Percent_Identity=28.2085561497326, Blast_Score=250, Evalue=3e-67,
Organism=Escherichia coli, GI171474008, Length=746, Percent_Identity=28.1501340482574, Blast_Score=246, Evalue=5e-66,
Organism=Escherichia coli, GI3868721, Length=718, Percent_Identity=20.4735376044568, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009010
- InterPro:   IPR006658
- InterPro:   IPR006657
- InterPro:   IPR006656
- InterPro:   IPR006655 [H]

Pfam domain/function: PF00384 Molybdopterin; PF01568 Molydop_binding [H]

EC number: 1.7.2.3 [C]

Molecular weight: Translated: 78371; Mature: 78371

Theoretical pI: Translated: 6.48; Mature: 6.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYSDKRIRKPAIRRSWLKRREKSDRTLRGQEDFVEVDWDVALDLVAEENRRVRDQYGPA
CCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHCCHHHHHHCCCC
GLFTGSYGWSSAGRLHHARSLVRRFYFSGGGGVDQLGNYSWGAAQFFLPYVIGTFSPLTG
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCC
RVTSWPSVVEHCELFIAFGGLALKNAQVSSGGSAEHSLKLWLEKLAQKGTPVINISPMRD
CCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCC
DCPAFVNAEWIPIRPNTDVALMLALAYEIQRLDGHDEAFLATHCVGYGQLADYIRGANDG
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCCCC
IAKTPDWASSITGIPAARIAQLAQQLIGVRSFMTCSYSVQRAHRGEQPYWMVIALSAMLG
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
QVGLPGGGFSFGHGSMNGVGNPRIDTPGPAMPVGKNPAGLAIPVARICDMLLQPGQPYQF
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCCCEEE
QGETHTYPDIHLVHWAGGNPFHHHQQLNRLVEGWQKPDTVIVQDIWWTPVAKMADIVLPV
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
TSSLERNDIGGSSRDRYVLAMHQAIAPQHQARHDFDIFADLADRLGYRETFTEGRDEMAW
HCCCCCCCCCCCCCCCEEEEHHHHHCCHHHCCCCHHHHHHHHHHCCCHHHHHCCHHHHHH
IRHIYQECGAAQRSHDVEWPDFETFWQQGYVGLPAPSKAFVFFDSFREDPQANPLQTPSG
HHHHHHHHCCHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
KIELFSEKIASYQYNDFAPHAEWKPPVEWLGAPLAEQWPLHLISIQPGDRLHSQLDPAPL
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCC
AQSNKTAGRETLYMHPLDAASRDITEGSQVQVSNLRGHCLAGVSITDGVTRGVVLMATGA
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCEEEECCEECCCCCCCEEEEEECC
WFDPGFGGTQHKIEQSGNPNVLTLDIGTSRLTQGPNAMSCLVEVALYE
CCCCCCCCCHHHHHCCCCCCEEEEECCHHHHCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MVYSDKRIRKPAIRRSWLKRREKSDRTLRGQEDFVEVDWDVALDLVAEENRRVRDQYGPA
CCCCCCHHCCHHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHCCHHHHHHCCCC
GLFTGSYGWSSAGRLHHARSLVRRFYFSGGGGVDQLGNYSWGAAQFFLPYVIGTFSPLTG
CEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHHHCCCCCCC
RVTSWPSVVEHCELFIAFGGLALKNAQVSSGGSAEHSLKLWLEKLAQKGTPVINISPMRD
CCCCCHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEECCCCC
DCPAFVNAEWIPIRPNTDVALMLALAYEIQRLDGHDEAFLATHCVGYGQLADYIRGANDG
CCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHCCCCCC
IAKTPDWASSITGIPAARIAQLAQQLIGVRSFMTCSYSVQRAHRGEQPYWMVIALSAMLG
CCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHH
QVGLPGGGFSFGHGSMNGVGNPRIDTPGPAMPVGKNPAGLAIPVARICDMLLQPGQPYQF
HCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECHHHHHHHHHCCCCCEEE
QGETHTYPDIHLVHWAGGNPFHHHQQLNRLVEGWQKPDTVIVQDIWWTPVAKMADIVLPV
CCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHH
TSSLERNDIGGSSRDRYVLAMHQAIAPQHQARHDFDIFADLADRLGYRETFTEGRDEMAW
HCCCCCCCCCCCCCCCEEEEHHHHHCCHHHCCCCHHHHHHHHHHCCCHHHHHCCHHHHHH
IRHIYQECGAAQRSHDVEWPDFETFWQQGYVGLPAPSKAFVFFDSFREDPQANPLQTPSG
HHHHHHHHCCHHHCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCC
KIELFSEKIASYQYNDFAPHAEWKPPVEWLGAPLAEQWPLHLISIQPGDRLHSQLDPAPL
CHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCCCCEEEEEECCCHHHHHHCCCCCC
AQSNKTAGRETLYMHPLDAASRDITEGSQVQVSNLRGHCLAGVSITDGVTRGVVLMATGA
CCCCCCCCCCEEEECCCCCCCCCCCCCCEEEEECCCCEEEECCEECCCCCCCEEEEEECC
WFDPGFGGTQHKIEQSGNPNVLTLDIGTSRLTQGPNAMSCLVEVALYE
CCCCCCCCCHHHHHCCCCCCEEEEECCHHHHCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Spontaneous Oxidation Product Of Biotin, D-Biotin D-Sulfoxide (Bso Or Bds) [C]

Specific reaction: Reduces A Spontaneous Oxidation Product Of Biotin, D-Biotin D-Sulfoxide (Bso Or Bds), Back To Biotin. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7733660 [H]