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The map label for this gene is lolB [H]

Identifier: 22126177

GI number: 22126177

Start: 2529154

End: 2529840

Strand: Direct

Name: lolB [H]

Synonym: y2292

Alternate gene names: 22126177

Gene position: 2529154-2529840 (Clockwise)

Preceding gene: 22126170

Following gene: 22126178

Centisome position: 54.97

GC content: 47.89

Gene sequence:

>687_bases
ATGTTGCCTCATCAGAAACCTGCCGCAGGTGAAAACCAGACGGCTGATAGGATTTGTACCCATATGCCCATGCGTAAAAG
ACATTTTTATCGCCTGCTGCCTTTAGCCTCTCTGCTGCTGGCTGCTTGTACAATCCCAGTATCAAAGGGACCAGCGACTA
GCCCTACGTCGCCACAGTGGCGTCAGCATGAACAACAGTTGCAGCAACTTGGCCAATTTGAAACTCGTGGCGCATTTGCT
TACCTGTCTGATAAACAGAAAGTTTATGCCAGATTCTTTTGGCAACAGACCTCTCCCGAGCGTTATCGTTTATTACTGAC
TAACCCGTTAGGCAGCACTGAGTTGGAGTTAGTTGTACAACCGGGTGTGACACAACTGACGGATAATCAAGGTAAGCGCT
ACGTCAGTGATGATCCGCAAGAGATGATTCAAAAGTTGACAGGCATGTCCATCCCACTGGAAAGCCTGCGCCAATGGATC
CTTGGCTTACCGGGCGATACCTCTGATTTTACCCTTGACGATAAATATCGCCTGAAGAAACTGACTTACCAACAAAATGG
GGTGACATGGGTTGTTGATTATCAAGAATACAACACGCAGGTCACGCCCTCCTTACCCAGCCGCCTGGAGCTGAATCAAG
ATGGGCAACGGATTAAACTGAAAATGGATAGCTGGACGATCAAATAA

Upstream 100 bases:

>100_bases
TCAATATAGAACAAGATATTGACGCTCTGCCTTCAGCCCGTTAGCGTGAGCACCTTAGATGTCTATTTGCAACGAATCAA
AACGTGTTGTTTTGCCTTAA

Downstream 100 bases:

>100_bases
CATGACTACCGCGAATCAACCTATATGTCCGTCACCAGCAAAATGGCCGTCACCGGCAAAATTGAATTTGTTTCTTTATA
TCACCGGGCAACGCGCCGAC

Product: outer membrane lipoprotein LolB

Products: 5-aminolevulinate; CoA; CO2 [C]

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MLPHQKPAAGENQTADRICTHMPMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQWRQHEQQLQQLGQFETRGAFA
YLSDKQKVYARFFWQQTSPERYRLLLTNPLGSTELELVVQPGVTQLTDNQGKRYVSDDPQEMIQKLTGMSIPLESLRQWI
LGLPGDTSDFTLDDKYRLKKLTYQQNGVTWVVDYQEYNTQVTPSLPSRLELNQDGQRIKLKMDSWTIK

Sequences:

>Translated_228_residues
MLPHQKPAAGENQTADRICTHMPMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQWRQHEQQLQQLGQFETRGAFA
YLSDKQKVYARFFWQQTSPERYRLLLTNPLGSTELELVVQPGVTQLTDNQGKRYVSDDPQEMIQKLTGMSIPLESLRQWI
LGLPGDTSDFTLDDKYRLKKLTYQQNGVTWVVDYQEYNTQVTPSLPSRLELNQDGQRIKLKMDSWTIK
>Mature_228_residues
MLPHQKPAAGENQTADRICTHMPMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQWRQHEQQLQQLGQFETRGAFA
YLSDKQKVYARFFWQQTSPERYRLLLTNPLGSTELELVVQPGVTQLTDNQGKRYVSDDPQEMIQKLTGMSIPLESLRQWI
LGLPGDTSDFTLDDKYRLKKLTYQQNGVTWVVDYQEYNTQVTPSLPSRLELNQDGQRIKLKMDSWTIK

Specific function: Plays a critical role in the incorporation of lipoproteins in the outer membrane after they are released by the lolA protein [H]

COG id: COG3017

COG function: function code M; Outer membrane lipoprotein involved in outer membrane biogenesis

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lolB family [H]

Homologues:

Organism=Escherichia coli, GI1787460, Length=207, Percent_Identity=63.768115942029, Blast_Score=287, Evalue=4e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004565 [H]

Pfam domain/function: PF03550 LolB [H]

EC number: NA

Molecular weight: Translated: 26282; Mature: 26282

Theoretical pI: Translated: 9.47; Mature: 9.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPHQKPAAGENQTADRICTHMPMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQW
CCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
RQHEQQLQQLGQFETRGAFAYLSDKQKVYARFFWQQTSPERYRLLLTNPLGSTELELVVQ
HHHHHHHHHHHCHHHCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEC
PGVTQLTDNQGKRYVSDDPQEMIQKLTGMSIPLESLRQWILGLPGDTSDFTLDDKYRLKK
CCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEECCHHHHHH
LTYQQNGVTWVVDYQEYNTQVTPSLPSRLELNQDGQRIKLKMDSWTIK
HEEECCCCEEEEEHHCCCCEECCCCCCCEEECCCCCEEEEEECCEECC
>Mature Secondary Structure
MLPHQKPAAGENQTADRICTHMPMRKRHFYRLLPLASLLLAACTIPVSKGPATSPTSPQW
CCCCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
RQHEQQLQQLGQFETRGAFAYLSDKQKVYARFFWQQTSPERYRLLLTNPLGSTELELVVQ
HHHHHHHHHHHCHHHCCEEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCEEEEEEC
PGVTQLTDNQGKRYVSDDPQEMIQKLTGMSIPLESLRQWILGLPGDTSDFTLDDKYRLKK
CCCCEECCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCEECCHHHHHH
LTYQQNGVTWVVDYQEYNTQVTPSLPSRLELNQDGQRIKLKMDSWTIK
HEEECCCCEEEEEHHCCCCEECCCCCCCEEECCCCCEEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Succinyl-CoA; glycine [C]

Specific reaction: Succinyl-CoA + glycine = 5-aminolevulinate + CoA + CO2 [C]

General reaction: Acyltransferases [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA