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The map label for this gene is phoA [H]

Identifier: 22126018

GI number: 22126018

Start: 2352615

End: 2354090

Strand: Reverse

Name: phoA [H]

Synonym: y2130

Alternate gene names: 22126018

Gene position: 2354090-2352615 (Counterclockwise)

Preceding gene: 22126022

Following gene: 22126017

Centisome position: 51.17

GC content: 49.73

Gene sequence:

>1476_bases
ATGACTGTTTTACAACAAGGAATTATATCGATGAACCGTGTACCTACTTTATCCGGCATAGCATTATCATCCGGCATAGC
ATTATCGACTCTGTTACTGTCCAGTGCGCTATTAACGCCTAATGCGATTGCGGCTGCTACGGGCTTATATGACCGTGCTG
CGCATGGTGATGTGACTCAATTTGGCGGCGCACGTCGTTTGACTGAAGACCAAACCCAGGCTCTGCGTGACTCGTTATCC
AATAAAACAGTAAAAAATGTCATTTTGTTGATTGGAGATGGGATGGGCGATTCTGAAATCACGTCTGCCCGTAACTATGC
GATGGGGGCCGGTGGTTTCTTTAAAGGCATTGATGCGCTGCCGCTGACCGGGCAATACACGCACTATGCACTCGATAAAA
AAACACAAAAACCAAATTATGTGACTGATTCTGCTGCATCAGCGACGGCCTGGTCATCTGGCGTAAAAACGTATAATGGT
GCGTTGGGGGTTGATGTTTTTGGTAAAGACCATGTTACGTTGCTGGAACTGGCCAAAAAAGCAGGCAAAGCGACGGGTAA
TGTCTCTACCGCTGAACTACAAGATGCCACTCCTGCTGCGCAGTTTGCCCACGTGACCGGGCGTAAGTGCTATGGCCCGG
AAGAAACCAGTGAGAAATGCAGTACCAATGCATTGGAAAAAGGGGGCCGTGGCTCTATTACTGAGCAAATGATTGAAGGG
CGCGCTGATGTCACGCTTGGCGGTGGTGCAGCATCATTCAGCCAGGTCGCTAAAGCCGGTGAGTGGAAAGACCAGTCGTT
GCGTGATCAGGCGTTGGCCCGCGGCTACGTCATTGTTGAGAACCTTGATGACCTGAACGCAATAAAACACGCTAATCAAC
AGAAACCTCTATTGGGCTTATTCTCGCCAGGTAATATGCCAGTGCGTTGGCAGGGGCCAAAAGCCTCTTATCATGGCAAT
CTGGATAAACCGCCAGTGGTGTGTGAAAACAATGCTGAGCGGACCAGCGACATTCCAACGTTGGCTGTCATGACAGAAAA
AGCCATTGACCTGCTGAAAACCAATGAGAATGGCTTCTTCTTGCAAGTTGAAGGGGCGTCGATTGATAAACAGGATCACT
CCGCTAACCCATGTGGTCAGTTTGGTGAAACGGTTGATCTGGATGAAGCGGTACAAAAAGCGCTGGAATTCGCCCGTGCT
GATGGCAACACTTTGGTGATTGTGACGGCTGACCATGCTCACTCCAGCCAAATTATCGAGCCTGAAGCGAAGGCTCCGGG
CCTGACTCAGGCATTAACGACCAAAGATGGCGCGGTCATGGCGATCAGCTACGGTAACTCTGAAGACGATTCTCAAGGCC
ATACTGGGTCACAATTGCGGATAGCGGCTTATGGTCCTCACGCGGCTAACGTCGTAGGTTTAACTGACCAAACTGATTTG
TTCTTCACCATGCGGGATGCGATGGCACTTAAATAA

Upstream 100 bases:

>100_bases
AAATGAGAGGGCAAACGTTTGAATGATAATTAGTGGTATCTGTCATAAAAGCGTCACCCGGCTGTTCTAGTATCCGTTGT
AACCAATCAATACAAATTGT

Downstream 100 bases:

>100_bases
GTCGCCTGTTATCTCACTGGAACGTCCCGGCGCAGTAATGGCCGGGATTTTTGCTTATGTCCCGTCACGGTAATCCTGCT
TATATCTGGAAAATTCTGTT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MTVLQQGIISMNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQFGGARRLTEDQTQALRDSLS
NKTVKNVILLIGDGMGDSEITSARNYAMGAGGFFKGIDALPLTGQYTHYALDKKTQKPNYVTDSAASATAWSSGVKTYNG
ALGVDVFGKDHVTLLELAKKAGKATGNVSTAELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEG
RADVTLGGGAASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGLFSPGNMPVRWQGPKASYHGN
LDKPPVVCENNAERTSDIPTLAVMTEKAIDLLKTNENGFFLQVEGASIDKQDHSANPCGQFGETVDLDEAVQKALEFARA
DGNTLVIVTADHAHSSQIIEPEAKAPGLTQALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDL
FFTMRDAMALK

Sequences:

>Translated_491_residues
MTVLQQGIISMNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQFGGARRLTEDQTQALRDSLS
NKTVKNVILLIGDGMGDSEITSARNYAMGAGGFFKGIDALPLTGQYTHYALDKKTQKPNYVTDSAASATAWSSGVKTYNG
ALGVDVFGKDHVTLLELAKKAGKATGNVSTAELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEG
RADVTLGGGAASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGLFSPGNMPVRWQGPKASYHGN
LDKPPVVCENNAERTSDIPTLAVMTEKAIDLLKTNENGFFLQVEGASIDKQDHSANPCGQFGETVDLDEAVQKALEFARA
DGNTLVIVTADHAHSSQIIEPEAKAPGLTQALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDL
FFTMRDAMALK
>Mature_490_residues
TVLQQGIISMNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQFGGARRLTEDQTQALRDSLSN
KTVKNVILLIGDGMGDSEITSARNYAMGAGGFFKGIDALPLTGQYTHYALDKKTQKPNYVTDSAASATAWSSGVKTYNGA
LGVDVFGKDHVTLLELAKKAGKATGNVSTAELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEGR
ADVTLGGGAASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGLFSPGNMPVRWQGPKASYHGNL
DKPPVVCENNAERTSDIPTLAVMTEKAIDLLKTNENGFFLQVEGASIDKQDHSANPCGQFGETVDLDEAVQKALEFARAD
GNTLVIVTADHAHSSQIIEPEAKAPGLTQALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDLF
FTMRDAMALK

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=496, Percent_Identity=28.0241935483871, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI94721246, Length=497, Percent_Identity=27.9678068410463, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI116734717, Length=407, Percent_Identity=29.97542997543, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI157266292, Length=473, Percent_Identity=26.8498942917548, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI294660770, Length=397, Percent_Identity=29.4710327455919, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI294660772, Length=345, Percent_Identity=29.8550724637681, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI48994877, Length=468, Percent_Identity=74.1452991452991, Blast_Score=716, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320689, Length=361, Percent_Identity=32.409972299169, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355151, Length=422, Percent_Identity=30.8056872037915, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921912, Length=380, Percent_Identity=30.2631578947368, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI18859923, Length=361, Percent_Identity=31.0249307479224, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24651554, Length=405, Percent_Identity=29.3827160493827, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355981, Length=363, Percent_Identity=30.5785123966942, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355149, Length=435, Percent_Identity=27.5862068965517, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585213, Length=385, Percent_Identity=29.6103896103896, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI85725200, Length=381, Percent_Identity=28.8713910761155, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI85815833, Length=381, Percent_Identity=28.8713910761155, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21358067, Length=393, Percent_Identity=28.2442748091603, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24657827, Length=393, Percent_Identity=29.7709923664122, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24651556, Length=371, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24657835, Length=397, Percent_Identity=27.2040302267003, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24657842, Length=382, Percent_Identity=26.7015706806283, Blast_Score=79, Evalue=9e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 51815; Mature: 51683

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLQQGIISMNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQ
CCHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHH
FGGARRLTEDQTQALRDSLSNKTVKNVILLIGDGMGDSEITSARNYAMGAGGFFKGIDAL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCC
PLTGQYTHYALDKKTQKPNYVTDSAASATAWSSGVKTYNGALGVDVFGKDHVTLLELAKK
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
AGKATGNVSTAELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEG
HCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCC
RADVTLGGGAASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGL
CCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCEEEE
FSPGNMPVRWQGPKASYHGNLDKPPVVCENNAERTSDIPTLAVMTEKAIDLLKTNENGFF
ECCCCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEHHHHHHHHHHEECCCCEE
LQVEGASIDKQDHSANPCGQFGETVDLDEAVQKALEFARADGNTLVIVTADHAHSSQIIE
EEEECCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PEAKAPGLTQALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDL
CCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCE
FFTMRDAMALK
EEEECHHHCCC
>Mature Secondary Structure 
TVLQQGIISMNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQ
CHHHHHHHHHCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHH
FGGARRLTEDQTQALRDSLSNKTVKNVILLIGDGMGDSEITSARNYAMGAGGFFKGIDAL
CCCCCCCCHHHHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCC
PLTGQYTHYALDKKTQKPNYVTDSAASATAWSSGVKTYNGALGVDVFGKDHVTLLELAKK
CCCCCCEEEEECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHH
AGKATGNVSTAELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEG
HCCCCCCCCHHHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCC
RADVTLGGGAASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGL
CCCEEECCCHHHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCEEEE
FSPGNMPVRWQGPKASYHGNLDKPPVVCENNAERTSDIPTLAVMTEKAIDLLKTNENGFF
ECCCCCCEEECCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEHHHHHHHHHHEECCCCEE
LQVEGASIDKQDHSANPCGQFGETVDLDEAVQKALEFARADGNTLVIVTADHAHSSQIIE
EEEECCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCC
PEAKAPGLTQALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDL
CCCCCCCCHHHHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCE
FFTMRDAMALK
EEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]