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The map label for this gene is vacJ [H]

Identifier: 22125471

GI number: 22125471

Start: 1742206

End: 1742982

Strand: Direct

Name: vacJ [H]

Synonym: y1576

Alternate gene names: 22125471

Gene position: 1742206-1742982 (Clockwise)

Preceding gene: 22125470

Following gene: 22125473

Centisome position: 37.87

GC content: 51.09

Gene sequence:

>777_bases
ATGGAGAAAAATATGAACTTCCGCCTTACTGGGTTGGCTTTCGCAAGCGTACTGTTAGTTGGGTGCGCCAGTAGTTCACC
GGATCACATGGAGCAGGGCCGCTCTGATCCGCTGGAAGGTTTCAACCGGGCAATGTTCAATTTTAACTATGAGGTCCTCG
ACCCTTATGTGGTGCGTCCGGTCGCCGTCGTCTGGCGTGATTATGTGCCGCAACCAGCACGTAACGGTATGAGCAACTTT
TTGGGTAACCTCGAAGAACCCGCCAGTATGGTGAACAGCTTCCTGGTCGGTGATCCATATAATGCGATGAAGCATTTTAA
CCGCTTCTTCCTGAATACCGTTTTGGGTATGGGCGGTTTGATCGATGTCGCCGGGATGGCTAATCCGAAACTGGCAAAAC
AAGTGCCTCAACGTTTTGGTAGTACATTAGGCCACTATGATGTGGGCTACGGCCCGTATGTGGTGCTACCAGGGTATGGC
AGTTTTACCCTCCGCGAAGATGGCGGCCAGTTGGCAGATACGGTGTATCCGGTACTGAGTTATCTGACGTTCTGGATGGC
CGCAGGGAAATGGGTGCTTGAAGGGGTTGAAACCCGCGCACAGCTATTGGACTCTGATGGTTTAGTGCGTAATTCTTCAG
ATCCTTATCTCATGATACGCGAGGCTTATTTCCAGCGTCATGATTTCCTGGCGAATGGTGGCCAACTGAAACCTGAAGTT
AACTCGAATGCTCAGGCTATTCAGGGCGATCTGGATAGCATAGATGCCGCCGATTGA

Upstream 100 bases:

>100_bases
CGGTGCAGGATTTCAAAGGCAATGGGCCGTCGAGTGAGCAAAAAATAGGCGTTCAAATAAATAAACAAATCCCTTAAGTG
AAAGGGATAGACAGACTTTC

Downstream 100 bases:

>100_bases
GACTCATTGATATTAACTGGTCTAGCGCAGTACTCACCGCCGTATTCAAAATAGAAAAGCGCCATCAGGCGCTTTTCTAT
TGGATAAGGTGATGAATGAC

Product: VacJ lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MEKNMNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRPVAVVWRDYVPQPARNGMSNF
LGNLEEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGLIDVAGMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYG
SFTLREDGGQLADTVYPVLSYLTFWMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEV
NSNAQAIQGDLDSIDAAD

Sequences:

>Translated_258_residues
MEKNMNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRPVAVVWRDYVPQPARNGMSNF
LGNLEEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGLIDVAGMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYG
SFTLREDGGQLADTVYPVLSYLTFWMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEV
NSNAQAIQGDLDSIDAAD
>Mature_258_residues
MEKNMNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRPVAVVWRDYVPQPARNGMSNF
LGNLEEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGLIDVAGMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYG
SFTLREDGGQLADTVYPVLSYLTFWMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEV
NSNAQAIQGDLDSIDAAD

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=252, Percent_Identity=75, Blast_Score=397, Evalue=1e-112,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 28500; Mature: 28500

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKNMNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRP
CCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHCCHHHHHH
VAVVWRDYVPQPARNGMSNFLGNLEEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGL
HHHHHHHHCCCHHHCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
IDVAGMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYGSFTLREDGGQLADTVYPVLS
HHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHH
YLTFWMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCC
NSNAQAIQGDLDSIDAAD
CCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MEKNMNFRLTGLAFASVLLVGCASSSPDHMEQGRSDPLEGFNRAMFNFNYEVLDPYVVRP
CCCCCCEEEHHHHHHHHHHHHCCCCCCHHHHCCCCCHHHHHHHHHHCCCHHHCCHHHHHH
VAVVWRDYVPQPARNGMSNFLGNLEEPASMVNSFLVGDPYNAMKHFNRFFLNTVLGMGGL
HHHHHHHHCCCHHHCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
IDVAGMANPKLAKQVPQRFGSTLGHYDVGYGPYVVLPGYGSFTLREDGGQLADTVYPVLS
HHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCEEEECCCCHHHHHHHHHHH
YLTFWMAAGKWVLEGVETRAQLLDSDGLVRNSSDPYLMIREAYFQRHDFLANGGQLKPEV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEEHHHHHHHHHHHHCCCCCCCCCC
NSNAQAIQGDLDSIDAAD
CCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]