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The map label for this gene is proP [H]

Identifier: 22124137

GI number: 22124137

Start: 232190

End: 233554

Strand: Reverse

Name: proP [H]

Synonym: y0217

Alternate gene names: 22124137

Gene position: 233554-232190 (Counterclockwise)

Preceding gene: 22124138

Following gene: 22124136

Centisome position: 5.08

GC content: 49.01

Gene sequence:

>1365_bases
ATGGAAACCTTAAGTGCTGACTCTGCTATTACAGCGGCAGAAAATGACCCATTGAGAGACGTGAAAAGGAAAAATGCCAT
TAAAGGCGCGTTCTTTTCCGAGTTTATAGATATGTTCGATATCTATCTGCCGGTTATTGTGTTGCCCCCCGTTTTGTTCT
ATTTCCAGCCACCTAATTTATCGAGCAGCACCGCTAATATCTTGGCTTCACTGGTCTTTATCACAACCCTGTTAGGCCGG
CCAATTGGCGCTTTACTGTTTGGGATTATGGCTGATCGTATTGGTCGTCGTATGGCATCGATTTATTCAGTATCAGGATT
TGGTGTGGTGACATTTTTGATTGCGCTCATTCCTGGCTACGAAACACTGGGGATCGCCTCTTATCTGTTGTTGGTATTAC
TGCGTTTTATCGACGGCATTTTTCTCGGCGGGGGATATACCGGTGCGATACCGCTTGCGCTCGAATATTCTAAAAAAGAA
CAGCGGGGATTTGTCGGGGGGTTGATCCTCAGTGGTTTCCCTGCAGCCTATGTCGCGATCAATTTGGTGGCGATGCTGAT
GTTCGCTCTGATCCCACTGGATGGGCTGTATTCACCGTATGCGCAGTGGGGCTGGCGTATCCCCTTTGTGATTGGTGGGT
TACTGGCCGGGTTCCTCGCCCTGTATTACGTGTTTAATGTCACGGAATCTGAGGTGTGGCAACAAGGCAGCAGCAAGAAA
CGCGCACGTGAAAAACAGCCTTTATCAACCTTAGTCAGTGGACAAAGTGGGCGTAATTTATGGCAGGTATTACTGATGAT
GAGCGGTTTTTGGCTGACGCAAAATCTTATCACCCTTTTTCTCCCCACCGGGCTGCTAATCAATACCCTGAATATGAGGG
GGCTACAGGTGACATCGATCTTGTTAGTGACCTATTGCGTGCTTTTCTTCAGCTATATCGGCATGGGCATGTTGGGGCAA
AAAATCGGGCGGCGGCGTTTCTTCATGATTGCAGGCCCCTTGATTGCAACTATGGGGTCAGCGCTGCTCTACGTATTAAG
TCATGGCGATGGCTTATCATTTAGTACCGTTATGCTGTTGGTCTGTCTATTGGCGGTAGTGGTGACCTCTCCCTGGGGGG
TGATCATCACGTATATTAATGAGCATTTCGCCACTGGCGTACGGGCTACTGGGTTTGGTGTTGGGTTTAGCTTATCCGTC
ATCATTCCCTCTTTTTATGCTTTCTATATGGACTGGCTCAGTGTCGTGGTGCCCTTTGAACTGACTGCGGTGGTGTTACT
GGCGCTGGGGGGGATGATAGGTACGGTGGGGGCCATTATGGGGCCGGAAACTAAAGAGGTCGATTTTACCTCGTCAGCGG
GCTAA

Upstream 100 bases:

>100_bases
CGATCAATAACATGCCCTCTGCGGTAGATTTCAATCTTATATCTCTATCAAGGATGCAGAGGAAAGAAAAGGATGTATAG
GAAAGAAGTAGGAGCCTGAA

Downstream 100 bases:

>100_bases
CGGGGTGATTATCACCGGGTCTTTTCTTGTTTTGTATTACAGAGGCATGGTACAAAAAGCAACCATATTGTCGGACAGGA
ATACAATTAATGCTAGCGGG

Product: metabolite transport protein, permease

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 454; Mature: 454

Protein sequence:

>454_residues
METLSADSAITAAENDPLRDVKRKNAIKGAFFSEFIDMFDIYLPVIVLPPVLFYFQPPNLSSSTANILASLVFITTLLGR
PIGALLFGIMADRIGRRMASIYSVSGFGVVTFLIALIPGYETLGIASYLLLVLLRFIDGIFLGGGYTGAIPLALEYSKKE
QRGFVGGLILSGFPAAYVAINLVAMLMFALIPLDGLYSPYAQWGWRIPFVIGGLLAGFLALYYVFNVTESEVWQQGSSKK
RAREKQPLSTLVSGQSGRNLWQVLLMMSGFWLTQNLITLFLPTGLLINTLNMRGLQVTSILLVTYCVLFFSYIGMGMLGQ
KIGRRRFFMIAGPLIATMGSALLYVLSHGDGLSFSTVMLLVCLLAVVVTSPWGVIITYINEHFATGVRATGFGVGFSLSV
IIPSFYAFYMDWLSVVVPFELTAVVLLALGGMIGTVGAIMGPETKEVDFTSSAG

Sequences:

>Translated_454_residues
METLSADSAITAAENDPLRDVKRKNAIKGAFFSEFIDMFDIYLPVIVLPPVLFYFQPPNLSSSTANILASLVFITTLLGR
PIGALLFGIMADRIGRRMASIYSVSGFGVVTFLIALIPGYETLGIASYLLLVLLRFIDGIFLGGGYTGAIPLALEYSKKE
QRGFVGGLILSGFPAAYVAINLVAMLMFALIPLDGLYSPYAQWGWRIPFVIGGLLAGFLALYYVFNVTESEVWQQGSSKK
RAREKQPLSTLVSGQSGRNLWQVLLMMSGFWLTQNLITLFLPTGLLINTLNMRGLQVTSILLVTYCVLFFSYIGMGMLGQ
KIGRRRFFMIAGPLIATMGSALLYVLSHGDGLSFSTVMLLVCLLAVVVTSPWGVIITYINEHFATGVRATGFGVGFSLSV
IIPSFYAFYMDWLSVVVPFELTAVVLLALGGMIGTVGAIMGPETKEVDFTSSAG
>Mature_454_residues
METLSADSAITAAENDPLRDVKRKNAIKGAFFSEFIDMFDIYLPVIVLPPVLFYFQPPNLSSSTANILASLVFITTLLGR
PIGALLFGIMADRIGRRMASIYSVSGFGVVTFLIALIPGYETLGIASYLLLVLLRFIDGIFLGGGYTGAIPLALEYSKKE
QRGFVGGLILSGFPAAYVAINLVAMLMFALIPLDGLYSPYAQWGWRIPFVIGGLLAGFLALYYVFNVTESEVWQQGSSKK
RAREKQPLSTLVSGQSGRNLWQVLLMMSGFWLTQNLITLFLPTGLLINTLNMRGLQVTSILLVTYCVLFFSYIGMGMLGQ
KIGRRRFFMIAGPLIATMGSALLYVLSHGDGLSFSTVMLLVCLLAVVVTSPWGVIITYINEHFATGVRATGFGVGFSLSV
IIPSFYAFYMDWLSVVVPFELTAVVLLALGGMIGTVGAIMGPETKEVDFTSSAG

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=415, Percent_Identity=26.9879518072289, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI1788292, Length=412, Percent_Identity=26.9417475728155, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1788942, Length=415, Percent_Identity=27.4698795180723, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1789941, Length=225, Percent_Identity=31.5555555555556, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI87082231, Length=247, Percent_Identity=30.7692307692308, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49293; Mature: 49293

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METLSADSAITAAENDPLRDVKRKNAIKGAFFSEFIDMFDIYLPVIVLPPVLFYFQPPNL
CCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
SSSTANILASLVFITTLLGRPIGALLFGIMADRIGRRMASIYSVSGFGVVTFLIALIPGY
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
ETLGIASYLLLVLLRFIDGIFLGGGYTGAIPLALEYSKKEQRGFVGGLILSGFPAAYVAI
HHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHCCHHHHHHH
NLVAMLMFALIPLDGLYSPYAQWGWRIPFVIGGLLAGFLALYYVFNVTESEVWQQGSSKK
HHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHH
RAREKQPLSTLVSGQSGRNLWQVLLMMSGFWLTQNLITLFLPTGLLINTLNMRGLQVTSI
HHHHHCHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LLVTYCVLFFSYIGMGMLGQKIGRRRFFMIAGPLIATMGSALLYVLSHGDGLSFSTVMLL
HHHHHHHHHHHHHCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VCLLAVVVTSPWGVIITYINEHFATGVRATGFGVGFSLSVIIPSFYAFYMDWLSVVVPFE
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTAVVLLALGGMIGTVGAIMGPETKEVDFTSSAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
METLSADSAITAAENDPLRDVKRKNAIKGAFFSEFIDMFDIYLPVIVLPPVLFYFQPPNL
CCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCC
SSSTANILASLVFITTLLGRPIGALLFGIMADRIGRRMASIYSVSGFGVVTFLIALIPGY
CCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCC
ETLGIASYLLLVLLRFIDGIFLGGGYTGAIPLALEYSKKEQRGFVGGLILSGFPAAYVAI
HHHHHHHHHHHHHHHHHHHEEECCCCCCCEEEEEECCCHHHCCHHHHHHHHCCHHHHHHH
NLVAMLMFALIPLDGLYSPYAQWGWRIPFVIGGLLAGFLALYYVFNVTESEVWQQGSSKK
HHHHHHHHHHHCCCCCCCCHHHHCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHH
RAREKQPLSTLVSGQSGRNLWQVLLMMSGFWLTQNLITLFLPTGLLINTLNMRGLQVTSI
HHHHHCHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
LLVTYCVLFFSYIGMGMLGQKIGRRRFFMIAGPLIATMGSALLYVLSHGDGLSFSTVMLL
HHHHHHHHHHHHHCHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
VCLLAVVVTSPWGVIITYINEHFATGVRATGFGVGFSLSVIIPSFYAFYMDWLSVVVPFE
HHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTAVVLLALGGMIGTVGAIMGPETKEVDFTSSAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]